Avian Pathology最新文献_第9页

Genetic characterization of avian polyomaviruses identified from psittacine birds in South Korea. 从韩国鹦鹉螺属鸟类中鉴定出的鸟类多瘤病毒的遗传特征。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-09-06 DOI: 10.1080/03079457.2023.2247347

Ye-Ji Yun, HyeSoon Song, Yong-Kuk Kwon, Choi-Kyu Park, Hye-Ryoung Kim

{"title":"Genetic characterization of avian polyomaviruses identified from psittacine birds in South Korea.","authors":"Ye-Ji Yun, HyeSoon Song, Yong-Kuk Kwon, Choi-Kyu Park, Hye-Ryoung Kim","doi":"10.1080/03079457.2023.2247347","DOIUrl":"10.1080/03079457.2023.2247347","url":null,"abstract":"Budgerigar fledgling disease (BFD) is a contagious disease caused by avian polyomavirus (APV) in psittacine birds and causes high mortality rates. Here, eight APV-positive cases were confirmed from dead parrots or parrot tissue samples by polymerase chain reaction (PCR). Full-length genome sequencing showed high nucleotide identity (98.84-100%) between the APV strains. Phylogenetic analysis revealed that two genogroups were cocirculating in South Korea. The nucleotide sequences of five strains, collected from different parrot species, were identical; however, pathological lesions were observed in only two parrots, both aged 2 months. Pathology included necrotic spots in the liver, subcutaneous haemorrhage, hepatomegaly, ascites, intranuclear inclusion bodies, hepatocyte karyomegaly, hepatic necrosis, and bile duct proliferation. This suggests that the pathogenicity of APV might be host age-dependent regardless of the host species. This study improves our understanding of APV pathogenicity and provides a more detailed genetic characterization of APV strains.RESEARCH HIGHLIGHTS Eight APV strains were identified in South Korea from 2019 to 2021.By phylogenetic analysis, South Korean APV strains were classified into two clades.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10513538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and phylogenetic analysis of Marek's disease virus in chickens from Ogun State, Nigeria. 尼日利亚奥贡州鸡马立克氏病病毒的分子特征和系统发育分析。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-08-22 DOI: 10.1080/03079457.2023.2243838

E B Oluwayinka, E B Otesile, O O Oni, O L Ajayi, J R Dunn

{"title":"Molecular characterization and phylogenetic analysis of Marek's disease virus in chickens from Ogun State, Nigeria.","authors":"E B Oluwayinka, E B Otesile, O O Oni, O L Ajayi, J R Dunn","doi":"10.1080/03079457.2023.2243838","DOIUrl":"10.1080/03079457.2023.2243838","url":null,"abstract":"Marek's disease (MD) is caused by oncogenic MD virus serotype 1 (MDV1) and is characterized by lymphoproliferative lesions resulting in high morbidity and mortality in chickens. Despite being ubiquitous on poultry farms, there is a dearth of information on its molecular characteristics in Nigeria. This study aimed at characterizing three virulence genes (Meq, pp38, and vIL-8) of MDV1 from chickens in Ogun state, Nigeria. Blood, feather quill, and tumour samples of chickens from different commercial poultry farms in Ogun State were pooled, spotted on 107 FTA cards, and screened for MDV1 by polymerase chain reaction (PCR). Phylogenetic analysis was carried out to compare Nigerian MDV1 Meq, pp38, and vIL-8 genes sequences with the published references. Thirteen samples were MDV1-positive and the Meq, as well as pp38, and vIL-8 genes from the different samples were 100% identical. The Meq genes contained 339 amino acids (aa) with three PPPP motifs in the transactivation domain and two interruptions of the PPPP motifs due to proline-to-arginine substitutions at positions 176 and 217 resulting in a 20.88% proline composition. Phylogenetic analysis revealed that the Meq gene clustered with strains from Egypt and very virulent ATE2539 strain from Hungary. Mutations were observed in the pp38 protein (at positions 107 and 109) and vIL-8 protein (at positions 4 and 31). Based on the molecular analysis of the three genes, the results indicate the presence of MDV1 with virulence signatures; therefore, further studies on in vivo pathotyping of Nigerian MDV1 from all states should be performed.RESEARCH HIGHLIGHTS Meq, pp38 and vIL-8 genes were 100% identical between Nigerian MDV strains.Proline content in Nigerian meq gene was 20.88% with two PPPP motifs interruptions.Meq, pp38 and vIL-8 genes of Nigerian MDV were similar to Egyptian and Indian strains.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10037249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay. 通过基于便携式CRISPR/Cas13a的侧流测定法目视检测家禽腺病毒血清型4。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-10-11 DOI: 10.1080/03079457.2023.2254253

Dongdong Yin, Lei Yin, Jieru Wang, Yin Dai, Xuehuai Shen, Ruihong Zhao, Kezong Qi, Xiaocheng Pan

{"title":"Visual detection of fowl adenovirus serotype 4 via a portable CRISPR/Cas13a-based lateral flow assay.","authors":"Dongdong Yin, Lei Yin, Jieru Wang, Yin Dai, Xuehuai Shen, Ruihong Zhao, Kezong Qi, Xiaocheng Pan","doi":"10.1080/03079457.2023.2254253","DOIUrl":"10.1080/03079457.2023.2254253","url":null,"abstract":"The widespread occurrence of fowl adenovirus serotype 4 (FAdV-4)-induced hepatitis-hydropericardium syndrome (HHS) has led to significant economic losses for the poultry industry. A sensitive, accurate, and practical FAdV-4 diagnostic approach is urgently required to limit the incidence of the disease. In the present study, a practical method for detecting FAdV-4 was developed using the CRISPR/Cas13a system and recombinase-aided amplification. The approach was based on 37°C isothermal detection with visible results being achieved. The detection limit of the target gene with this approach was only 101 copies/μl, making it very sensitive and specific. Clinical samples fared well when tested with the Cas13a detection method. For identifying FAdV-4, this novel detection approach was found to be sensitive, specific, and effective.RESEARCH HIGHLIGHTS First study using the CRISPR/Cas13a-based lateral flow detection assay for FAdV-4 detection.The results can be observed by the naked eye.The developed assay could provide an alternative tool for detection of FAdV-4 with minimal equipment.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41117077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis of Newcastle disease virus detected in Eritrea between 2017 and 2021. 2017年至2021年间在厄立特里亚检测到的新城疫病毒的系统发育分析。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-08-24 DOI: 10.1080/03079457.2023.2247370

Bereket Mihreteab, Tebogo Kgotlele, Fitsum Neguse, Yodahi Petros, Habtom Habtemariam, Yordanos Berhane, Munir Wehab, Giovanni Cattoli, Irene K Meki, William G Dundon

引用次数: 0

Morphological, molecular, and pathological studies on Prosthogonimus cuneatus in Indian peacocks (Pavo cristatus). 印度孔雀（Pavo critatus）楔状前胸的形态学、分子生物学和病理学研究。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-09-05 DOI: 10.1080/03079457.2023.2251917

Asok Kumar Mariappan, Megha Sharma, Karikalan Mathesh, Vivek Srinivas Mouttou, Hiraram, Abhijeet Pawde, Dhama Kuldeep, G Saikumar

{"title":"Morphological, molecular, and pathological studies on Prosthogonimus cuneatus in Indian peacocks (Pavo cristatus).","authors":"Asok Kumar Mariappan, Megha Sharma, Karikalan Mathesh, Vivek Srinivas Mouttou, Hiraram, Abhijeet Pawde, Dhama Kuldeep, G Saikumar","doi":"10.1080/03079457.2023.2251917","DOIUrl":"10.1080/03079457.2023.2251917","url":null,"abstract":"This report provides the first record of Prosthogonimus cuneatus infection in Indian peafowl. Chickens, turkeys, geese, ducks, and other pet birds are recognized as direct hosts of Prosthogonimus species; however, P. cuneatus has not been reported to infect peafowl globally. Here, we identify the trematode present in the bursa of the peafowl by both morphological and molecular methods, in addition to the changes in the bursal tissue induced by the parasite, using histopathology. After a necropsy examination, the trematodes were found in the bursa of Fabricius in three peafowl. Morphological and molecular approaches based on taxonomic characteristics and the sequencing of the trematode-specific internal transcribed spacer (ITS) gene, respectively, were employed for trematode identification. The consensus sequences were compared to P. cuneatus reference sequences from GenBank. In order to assess the pathology caused by the parasite, a histological study of the bursa was also performed. Trematodes were confirmed as P. cuneatus based on morphology and DNA sequencing. Further, histopathological evaluation revealed mild lymphoid depletion of the bursal follicles in both the cortex and medulla with associated thinning of the bursal follicular lining epithelium. Indian peafowl can act as a natural host of P. cuneatus. This study provides a detailed pathological and molecular analysis of P. cuneatus affecting Indian peafowl.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10522495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-circulation of multiple genotypes of ARV in poultry in Anhui, China. 安徽地区家禽抗逆转录病毒多基因型的共循环研究。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-08-29 DOI: 10.1080/03079457.2023.2226081

Xiaoning Jiang, Feng Wei, Dalin He, Xing Niu, Bingrong Wu, Qiong Wu, Yi Tang, Youxiang Diao

{"title":"Co-circulation of multiple genotypes of ARV in poultry in Anhui, China.","authors":"Xiaoning Jiang, Feng Wei, Dalin He, Xing Niu, Bingrong Wu, Qiong Wu, Yi Tang, Youxiang Diao","doi":"10.1080/03079457.2023.2226081","DOIUrl":"10.1080/03079457.2023.2226081","url":null,"abstract":"ABSTRACTPoultry production in China has been experiencing a high incidence of broiler arthritis /tenosynovitis caused by avian orthoreovirus (ARV) since 2013. In the spring of 2020 severe arthritis cases from broiler flocks were identified in a large-scale commercial poultry company in Anhui Province, China. Diseased organs from dead birds were sent for diagnosis to our laboratory. ARVs, including seven broiler-isolates and two breeder-isolates, were successfully harvested and sequenced. Interestingly, the genotypes of ARVs isolated from infected chickens were inconsistent between different flocks, or even between different houses on the same flocks. Pathogenicity testing in chicks confirmed that the seven broiler-isolates were pathogenic strains, which could cause arthritis in infected chickens. Subsequently, a total of 89.66% serum samples collected from apparently healthy adult broiler flocks not vaccinated against ARV tested positive for ARV antibodies, suggesting that low and high virulence reovirus strains may be co-circulating in the farm. To this end, we collected dead embryos of unhatched chicken eggs for pathogen tracing, and the two ARV breeder-isolates isolated indicated that vertical transmission from breeders to progeny should not be underestimated for the prevalence of ARV within broiler flocks. The findings have implications for the evidenced-based formulation of prevention and control strategies.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10154345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

siRNA targeting Atp5a1 gene encoding ATPase α, the ligand of Peg fimbriae, reduced Salmonella Enteritidis adhesion. siRNA靶向编码飞毛菌配体ATP酶α的Atp5a1基因，降低了肠炎沙门氏菌的粘附。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-09-04 DOI: 10.1080/03079457.2023.2243842

Di Zhang, Yi Jiang, Yongyi Dong, Lixia Fu, Linlin Zhuang, Kun Wu, Xinhong Dou, Bu Xu, Chengming Wang, Jiansen Gong

{"title":"siRNA targeting Atp5a1 gene encoding ATPase α, the ligand of Peg fimbriae, reduced Salmonella Enteritidis adhesion.","authors":"Di Zhang, Yi Jiang, Yongyi Dong, Lixia Fu, Linlin Zhuang, Kun Wu, Xinhong Dou, Bu Xu, Chengming Wang, Jiansen Gong","doi":"10.1080/03079457.2023.2243842","DOIUrl":"10.1080/03079457.2023.2243842","url":null,"abstract":"Salmonella enterica serovar Enteritidis (S. Enteritidis) is a zoonotic pathogen that can infect both humans and animals. Among the 13 types of fimbrial operons in S. Enteritidis, the highly conserved Peg fimbriae play a crucial role in the adhesion and invasion of S. Enteritidis into host cells but are not well studied. In this study, we identified the ATP synthase subunit alpha (ATPase α) as a ligand of Peg fimbriae using ligand blotting and mass spectrometry techniques. We confirmed the in vitro binding of ATPase α to the purified adhesion protein (PegD). Furthermore, we used siRNA to suppress the expression of ATPase α gene Atp5a1 in Leghorn male hepatoma (LMH) cells, which resulted in a significant reduction in the adhesion rate of S. Enteritidis to the cells (P < 0.05). The findings in this study provide insight into the mechanism of S. Enteritidis infection through Peg fimbriae and highlight the importance of ATPase α in the adhesion process.RESEARCH HIGHLIGHTS Ligand blotting was performed to screen the ligand of S. Enteritidis Peg fimbriae.Binding assay confirmed that ATPase α is the ligand of the Peg fimbriae.siRNA targeting ATPase α gene (Atp5a1) significantly reduced S. Enteritidis adhesion.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10141207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Avian Diseases 67.2 Table of Contents. 67.2禽疾病目录。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-12-01 Epub Date: 2023-10-11 DOI: 10.1080/03079457.2023.2267285

引用次数: 0

International Meetings, Wvpa Matters and Announcements 国际会议、Wvpa事宜及公告

2区农林科学

Avian Pathology Pub Date : 2023-10-11 DOI: 10.1080/03079457.2023.2267276

引用次数: 0

Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus. 禽流感病毒H9N2亚型神经氨酸酶蛋白催化活性结构域表位的鉴定。

IF 2.8 2区农林科学

Avian Pathology Pub Date : 2023-10-01 Epub Date: 2023-08-15 DOI: 10.1080/03079457.2023.2239191

Xiangyu Huang, Yiqin Cai, Guihu Yin, Zili Chen, Jianing Hu, Zichen Gao, Xinyu Guo, Fuqiang Xiong, Xiuli Feng

{"title":"Identification of catalytically active domain epitopes in neuraminidase protein of H9N2 subtype of avian influenza virus.","authors":"Xiangyu Huang, Yiqin Cai, Guihu Yin, Zili Chen, Jianing Hu, Zichen Gao, Xinyu Guo, Fuqiang Xiong, Xiuli Feng","doi":"10.1080/03079457.2023.2239191","DOIUrl":"10.1080/03079457.2023.2239191","url":null,"abstract":"H9N2 subtype of avian influenza virus (AIV) is primarily a bird virus, which is widespread in clinical avian disease, and reported in cases of human infection. As one of the surface proteins of AIV, the neuraminidase (NA) protein plays an important role mainly in viral budding. However, vaccine development and detection methods for NA of H9N2 AIVs are in urgent clinical need. In this study, a truncated NA gene (205-900 bp) was cloned from the NA sequence of H9N2 strain, and then expressed using pET-28a (+) vector. This purified recombinant NA protein was used to immunize BALB/c mice, and the monoclonal antibodies were screened through the indirect enzyme-linked immunosorbent assay (ELISA). Next, eight prokaryotic expression vectors were constructed for epitope identification. After cell fusion, three hybridoma cell lines producing the antibodies special to NA protein were screened by ELISA, western blotting, and indirect immunofluorescence; these were named 1B10, 2B6, and 5B2, respectively. Epitope scanning techniques were used to identify three B-cell epitopes recognized by these three monoclonal antibodies, 196KNATASIIYDGMLVD210, 210DSIGSWSKNIL220 and 221RTQESECVCI230. The subsequent homology analysis revealed the three epitopes were highly conserved in H9N2 AIV strains. The structural predictions of the antigenic epitopes indicated that all three epitopes were located in the catalytic region of NA. These results provide a basis for studying the function of the NA protein of H9N2 AIV and technical support for the development of a universal detection method based on anti-NA monoclonal antibodies.","PeriodicalId":8788,"journal":{"name":"Avian Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10103400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0