Longitudinal Study of Avian Pathogenic Escherichia coli (APEC) serogroups associated with disease in Georgia poultry using molecular serology and virulence gene analysis.

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES

Avian Pathology Pub Date : 2024-09-12 DOI:10.1080/03079457.2024.2403414

Klao Runcharoon,Bellanirys Garcia,Breck N Peterson,Meaghan M Young,Margaret E Favro,Nicolle L Barbieri,Doug Waltman,Bridgeth Flores,Emily Dinh,Catherine M Logue

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引用次数: 0

Abstract

Avian pathogenic Escherichia coli (APEC) is a significant cause of morbidity, mortality, and production loss to the poultry industry worldwide. Here, we characterized 569 E. coli isolates from avian-diagnosed colibacillosis cases from the state of Georgia, USA. A total of 339 isolates were assigned into 32 serogroups with the majority classifying as O78, O2, O25, O8, O1, O86, O18, and O15. Serogroup O25 was found to link with broilers, while broiler breeders were more often associated with serogroup O1 and pet/ hobby birds with serogroup O8. In addition, some serogroups (O1) were more prevalent in the Summer and Fall. Analysis for virulence-associated genes (VAGs) found 23.20% of isolates did not harbor any genes linked with the APEC pathotype, while ColV plasmid-associated genes (iroN, ompT, hlyF, iss, and aerJ,) were frequently detected among most isolates (with 80 to 96% prevalence) and some of these genes were linked with serogroup. Phylogenetic analysis, classified isolates into phylogenetic groups B2 (27%), G (21%), F (15%), and A (11%). The phylogenetic group B2 isolates also harbored the highest number of VAGs. This study highlights that the current APEC-causing disease in birds in the State of Georgia has identified several emerging serogroups possessing several VAGs that could potentially lead to challenges in colibacillosis control.

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利用分子血清学和毒力基因分析对与格鲁吉亚家禽疾病相关的禽致病性大肠杆菌 (APEC) 血清群进行纵向研究。

禽致病性大肠杆菌 (APEC) 是导致全球家禽业发病、死亡和生产损失的一个重要原因。在此，我们对来自美国佐治亚州禽类确诊大肠杆菌病病例中的 569 个大肠杆菌分离物进行了鉴定。共有 339 个分离株被归入 32 个血清群，其中大多数被归入 O78、O2、O25、O8、O1、O86、O18 和 O15 血清群。发现血清 O25 群与肉鸡有关，而肉鸡种鸡与血清 O1 群有关，宠物/业余鸟类与血清 O8 群有关。此外，某些血清群（O1）在夏季和秋季更为流行。毒力相关基因（VAGs）分析发现，23.20%的分离株不携带任何与 APEC 病型相关的基因，而 ColV 质粒相关基因（iroN、ompT、hlyF、iss 和 aerJ）在大多数分离株中被频繁检测到（流行率为 80% 至 96%），其中一些基因与血清群相关。系统发生分析将分离株分为系统发生组 B2（27%）、G（21%）、F（15%）和 A（11%）。系统发育组 B2 分离物中的 VAG 数量也最多。这项研究强调，目前在格鲁吉亚州发生的导致鸟类感染 APEC 的疾病已经发现了几个新出现的血清群，这些血清群拥有多种 VAG，可能会给大肠杆菌病的控制带来挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.