Biochemical and biophysical research communications最新文献

{"title":"Chronic social defeat stress alters DNA methylation profiles of male germ cells in mice","authors":"Hikari Ohno,&nbsp;Yutaka Yamamuro,&nbsp;Shu Aizawa","doi":"10.1016/j.bbrc.2025.151650","DOIUrl":"10.1016/j.bbrc.2025.151650","url":null,"abstract":"<div><div>Chronic exposure to stress disrupts various structural and functional features in living organisms. In addition to the adverse effects of chronic stress on the central nervous system, evidence suggests that chronic stress leads to dysfunction of the male reproductive system. In particular, previous reports have shown that exposure to chronic social defeat stress (cSDS), an animal model of psychosocial stress and depression, induces a reduction in sperm concentration and motility. However, the mechanism by which exposure to cSDS contributes to male reproductive abnormalities remains poorly understood. In the present study, we investigated the effects of cSDS on DNA methylation profiles in germ cells isolated from the testes of C57BL/6J mice. We found that exposing mice to cSDS significantly decreased the 5-methylcytosine levels but not 5-hydroxymethylcytosine levels in germ cells. Furthermore, reduced representation of bisulfite sequencing revealed that cSDS exposure specifically alters the DNA methylation status at gene regulatory regions of transcriptional regulation-related genes. These findings suggest that chronic exposure to psychosocial stress disrupts the male reproductive system through abnormal epigenetic modifications in male germ cell, providing novel insight into the detrimental effects of chronic stress on male germ cell development in the testis.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151650"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-216a-3p alleviates primary Sjögren's syndrome by regulating the STAT1/JAK signaling pathway","authors":"Yixuan Liu ,&nbsp;Liuxiong Guo ,&nbsp;Jingjing Cao ,&nbsp;Xiaoran Ning ,&nbsp;Xiao Zheng ,&nbsp;Fang Li ,&nbsp;Guangyao Song","doi":"10.1016/j.bbrc.2025.151647","DOIUrl":"10.1016/j.bbrc.2025.151647","url":null,"abstract":"<div><h3>Background</h3><div>Sjögren's syndrome (SS) is a chronic systemic autoimmune It chiefly impacts the exocrine glands, specifically the salivary and lacrimal ones, causing manifestations such as dry mouth and eye. Sjögren's syndrome often coexists with other autoimmune diseases, making it difficult to study its pathogenesis. Mounting evidence suggests that microRNAs (miRNAs) play a pivotal role in the development of autoimmune diseases, yet the precise mechanisms underlying their involvement in SS remain to be fully elucidated.</div></div><div><h3>Methods</h3><div>A cohort dataset pertaining to Sjögren's syndrome was procured from the Gene Expression Omnibus (GEO) database and subsequently analyzed using bioinformatics tools. The association between Signal Transducer and Activator of Transcription 1 (STAT1) and Sjögren's syndrome is well-established. To predict miRNAs targeting STAT1, we utilized the TargetScan database, focusing on miR-216a-3p. Furthermore, to model primary Sjögren's syndrome (pSS) in vivo, we employed a rat model established through submandibular gland protein immunization. These pSS model rats were then subjected to injections of either miR-216a-3p mimics or inhibitors. Subsequently, histological analysis was conducted to assess the resulting tissue morphology. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was employed to determine the expression levels of both STAT1 and miR-216a-3p. Cytokine levels were quantified using enzyme-linked immunosorbent assay (ELISA). To investigate the protein expression of key components of the STAT/JAK signaling pathway, Western blot analysis was performed, targeting Signal Transducer and Activator of Transcription 1 (STAT1), Janus kinase 1 (JAK1), and Janus kinase 2 (JAK2).</div></div><div><h3>Results</h3><div>Our findings indicate that miR-216a-3p exerts regulatory control over the JAK/STAT signaling pathway by modulating the phosphorylation of STAT1, thereby attenuating the severity of primary Sjögren's syndrome in our model. Moreover, miR-216a-3p was observed to inhibit the secretion of pro-inflammatory cytokines and mitigate B cell hyperactivation. These results collectively suggest a potentially significant role for miR-216a-3p in the pathogenesis of autoimmune diseases, warranting further investigation.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151647"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Edible ginseng-derived exosomes as drug delivery vehicles reduce the dose and improve the anti-cancer effect of CDDP","authors":"Shuiyue Yang ,&nbsp;Jia Guo ,&nbsp;Shan Huang ,&nbsp;Zepeng Sun ,&nbsp;Min Yang ,&nbsp;Yinghua Peng","doi":"10.1016/j.bbrc.2025.151658","DOIUrl":"10.1016/j.bbrc.2025.151658","url":null,"abstract":"<div><div>Nanotechnology and nanomaterials have emerged as promising tools for the delivery of anti-tumor drug cisplatin (CDDP). However, concerns exist regarding potential toxicity and cost-effectiveness, limiting their clinical applications. In contrast, plant-derived exosomes (PDEs), as natural nanovesicles, offer significant advantages as drug delivery carriers due to their large-scale production, biocompatibility, and ability to efficiently transport therapeutic drug across cellular barriers. In this work, we established a ginseng-derived exosome (G-Exo)-based CDDP delivery system (G-CDDP) and evaluated its anti-tumor efficacy both <em>in vitro</em> and <em>in vivo</em>. The results demonstrated that G-CDDP effectively targeted tumor site, inhibiting the proliferation and migration and promoting apoptosis in U-87MG tumor cells. Notably, the amount of CDDP in G-CDDP required for achieving the same cytotoxic effect on tumor cells was 12.66 times lower than that of free CDDP. In U-87MG tumor-bearing mice, G-CDDP effectively targeted tumor sites and exhibited significant therapeutic effect. Collectively, these findings highlight the potent anti-tumor activity of G-CDDP at reduced CDDP dosage, positioning it as a promising and efficient alternative to conventional drug treatments in clinical settings.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151658"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the binding site and immunoreactivity of anti-Aβ antibody 11A1: Comparison with the toxic conformation-specific TxCo-1 antibody","authors":"Rara Fukui ,&nbsp;Uswah Hafizal ,&nbsp;Yusuke Kageyama ,&nbsp;Yumi Irie ,&nbsp;Yuka Matsushima ,&nbsp;Katsuma Hosoi ,&nbsp;Takahisa Nakayama ,&nbsp;Daita Kaneda ,&nbsp;Yoshio Hashizume ,&nbsp;Kunio Miki ,&nbsp;Akiko Kita ,&nbsp;Ken-ichi Mukaisho ,&nbsp;Ryoji Kushima ,&nbsp;Ikuo Tooyama ,&nbsp;Kazuhiro Irie","doi":"10.1016/j.bbrc.2025.151655","DOIUrl":"10.1016/j.bbrc.2025.151655","url":null,"abstract":"<div><div>Since the advent of anti-amyloid β (Aβ) immunotherapy, exemplified by lecanemab, the development of effective therapeutic agents with minimal side effects has become an urgent priority. Over the past two decades, a number of antibodies have been developed that target toxic Aβ species. The 11A1 antibody is one such example, and is made from E22P-Aβ9–35, which is prone to adopt a toxic conformation with a turn at positions 22/23, as an antigen. This antibody is unique in that it stains not only extracellular but also intracellular Aβ in human AD brains. To identify its recognition domain, we performed X-ray crystallography of 11A1 in complex with E22P-Aβ10–34. We found that 11A1 is a novel N-terminal antibody that recognizes Tyr10–His14 of Aβ. Immunohistochemical studies showed that 11A1 stains senile plaques and vascular Aβ aggregates in brain samples of AD patients. On the other hand, 11A1 recognized Aβ aggregates in neurons, astrocytes, perivascular tissue, and microvesicles of non-AD patients, suggesting that 11A1 can detect a wide range of Aβ types regardless of AD pathology. In contrast, the recently developed TxCo-1 antibody, which specifically recognizes the toxic turn at positions 22/23 of Aβ42, stained only senile plaques and vascular Aβ aggregates from AD patients, but not Aβ species from non-AD patients. These results suggest that the toxic turn structure may be one of the key epitopes for achieving high affinity for pathological Aβ aggregates while minimizing nonspecific binding to aggregates unrelated to pathology.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151655"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delving into the crucial role of the initial structure in the dynamic and self-assembly of amyloid beta","authors":"Maryam Ghorbaninia ,&nbsp;Shirin Doroudgar ,&nbsp;Mohamad Reza Ganjalikhany","doi":"10.1016/j.bbrc.2025.151652","DOIUrl":"10.1016/j.bbrc.2025.151652","url":null,"abstract":"<div><div>Alzheimer's disease involves the accumulation of amyloid beta (Aβ) monomers that form oligomers and fibrils in the brain. Studying the Aβ monomer is critical for understanding Aβ assembly and peptide behavior and has implications for drug design. Choosing a starting structure with a higher aggregation tendency for cost-effective MD studies and drug design is crucial. Previous studies have utilized distinct initial conformations, leading to varying results. Hence, this study was conducted to compare different initial conformations using the same MD simulation protocol to investigate the behavior and oligomerization propensity of different starting structures of Aβ during 1μs. The behavior of the monomers and their self-assembly systems were studied thoroughly, and the results revealed that highly helical Aβ monomers which used as starting structures retain high helix content during the simulation, and their tautomerization states did not cause significant changes in the structure. On the other hand, the Aβ extended and S-shaped monomers displayed the fingerprints of the fibril structure, which is believed to be more favorable for self-assembly. Self-assembly behaviors were seen for three S-shaped and three Aβ extended peptides. However, both conformations did not show stable β-sheet intermolecular interaction. For the Aβ16-22 monomer as a fragment of the Aβ that can assemble into fibrils, the impacts of capping and uncapping on the initial structure were also investigated. The results displayed that capped and uncapped structures can form oligomers with β-sheet at termini. However, in the capped state, β-sheet interactions were more stable and remained relatively longer than uncapped.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151652"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining interactions of animal cells with chloroplasts and their light-induced responses in in vitro cell culture systems","authors":"Kyota Hamashima ,&nbsp;Lilingman Fan ,&nbsp;Reika Miyagawa ,&nbsp;Natsuki Hara ,&nbsp;Kazuki Nishida ,&nbsp;Hisato Saitoh","doi":"10.1016/j.bbrc.2025.151622","DOIUrl":"10.1016/j.bbrc.2025.151622","url":null,"abstract":"<div><div>Chloroplasts are organelles that convert light energy into chemical energy in plants. The potential to integrate chloroplasts into animal cells presents an exciting frontier in synthetic biology, allowing for photo-controllable biochemical processes within these cells. However, the lack of well-established <em>in vitro</em> experimental systems to study chloroplast-animal cell interactions remains a significant challenge. This study investigates the behavior of human cervical cancer HeLa cells and mouse macrophage-like J774.1 cells, along with the light-induced responses of these cells, when introduced into culture media containing spinach-derived chloroplasts. Additionally, we examine isolated cells from <em>Elysia marginata</em>, a sacoglossan sea slug known for its unique ability to acquire and retain functional chloroplasts through a process known as kleptoplasty. Our results show that HeLa cells primarily adhere to chloroplasts with minimal intracellular uptake, while J774.1 cells actively engulf them. Co-incubation with chloroplasts increases the rate of cell death upon light irradiation. In contrast, naturally chloroplast-containing cells from <em>E. marginata</em> exhibit minimal light-induced damage. Excessive reactive oxygen species (ROS) production is observed in HeLa and J774.1 cells co-incubated with chloroplasts under light exposure, suggesting that photoinduced ROS generation contributes to cytotoxicity. These findings highlight three different patterns of interactions between animal cells and chloroplasts and underscore the importance of considering ROS generation induced by light exposure when analyzing chloroplast-animal cell interactions in <em>vitro</em> experimental systems.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151622"},"PeriodicalIF":2.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The TT8 transcription factor alleviates nickel toxicity in Arabidopsis","authors":"Yuhao Hu ,&nbsp;Yuxing Peng ,&nbsp;Xiaoting Qi","doi":"10.1016/j.bbrc.2025.151649","DOIUrl":"10.1016/j.bbrc.2025.151649","url":null,"abstract":"<div><div>Nickel (Ni) is a necessary element for plants, but excessive accumulation of Ni in soil causes plant damage, one of which is oxidative stress. Plants utilize secondary metabolite polyphenols such as anthocyanins as antioxidant molecules and metal chelators to cope with heavy metal toxicity. However, the regulatory factors linking polyphenol accumulation to resistance to Ni stress in plants remain unidentified. <em>Arabidopsis</em> TRANSPARENT TESTA8 (TT8) is an essential transcription factor involved in anthocyanin biosynthesis, especially in seeds. Here, we reported that TT8 links polyphenol accumulation to <em>Arabidopsis</em> tolerance to Ni stress. <em>TT8</em> knockout reduced the anthocyanin content in the seed coat, leading to several aspects of Ni toxicity: low seedling survival rates, delayed seed germination, severe membrane lipid peroxidation damage, reduced antioxidant capacity, and low polyphenol (especially epicatechin) abundance. When <em>TT8</em> was overexpressed, these <em>Arabidopsis</em> plants tolerated extreme Ni stress with high survival rates and germination rates and accumulated more polyphenols. On the basis of these data, we concluded that TT8 maintains the antioxidant capacity of <em>Arabidopsis</em> to avoid Ni stress–induced oxidative damage by promoting polyphenol accumulation. Moreover, TT8 not only responds to Ni stress but also positively regulates six flavonoid biosynthesis enzyme-encoding genes (<em>CHS</em>, <em>CHI</em>, <em>F3H</em>, <em>F3ˊH</em>, <em>DFR</em>, and <em>FLS</em>). Therefore, TT8-mediated transcriptional regulation cascades of flavonoid biosynthesis may contribute to its effects on polyphenol accumulation. Collectively, our findings provide deep mechanical insights into how specific transcription factors alleviate excessive Ni toxicity in plants and offer new approaches for the breeding of Ni stress-tolerant crops.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151649"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the antimicrobial activity of carvacrol and its mechanism of action against drug-resistant bacteria","authors":"Ziling Zhi ,&nbsp;Peng Zhou ,&nbsp;Tenghui He ,&nbsp;Sisi Chen ,&nbsp;Xiping Qian ,&nbsp;Yanyan Ye ,&nbsp;Wing-Leung Wong ,&nbsp;Song Li ,&nbsp;Ning Sun ,&nbsp;Wenchang Yuan","doi":"10.1016/j.bbrc.2025.151643","DOIUrl":"10.1016/j.bbrc.2025.151643","url":null,"abstract":"<div><div>Drug-resistant bacterial infections have been one of the critical health issues encountered worldwide currently because most conventional antibiotics are losing their effectiveness in clinical treatments. It is thus urgently to identify new antibiotics or alternatives against drug-resistant bacteria. For this purpose, we attempted to seek active compounds from commercially available natural products, which may be one of the fast-tracks to address the drug-resistant bacterial infections. In the present study, we investigated the antibacterial activity, antibacterial mechanism and synergistic effects of carvacrol against a panel of drug-resistant bacteria, including some clinical isolates. The results show that carvacrol (<em>cymophenol</em>), a monoterpenoid phenol, has excellent antibacterial activity. The MIC values against the bacteria examined are found to be 4–16 μg/mL. Our results also suggested that carvacrol might not likely to induce drug-resistance. More importantly, when carvacrol combined with first-line antibiotics, it exhibited good synergistic effects against drug-resistant bacteria. Moreover, in morphological studies, carvacrol could cause <em>B. subtilis</em> 168 elongation and <em>S. aureus</em> BAA-41 enlargement, which may suggest an antibacterial mechanism possibly correlated with the inhibition of bacterial cell division. We further demonstrated that carvacrol facilitated the polymerization of FtsZ that is a critically important protein for regulating bacterial cell division. Furthermore, molecular modeling predicted that carvacrol could interact with T7-loop of FtsZ. The findings of this study suggest that carvacrol may be a potential inhibitor of FtsZ and it could be utilized to combat drug-resistant bacteria in combination with existing antibiotics.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151643"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-targeting antimicrobial compounds have differential effects on living and artificial yeast membrane models","authors":"Jennifer I. Villacres ,&nbsp;Olivia Luong ,&nbsp;Michael Shaikhet ,&nbsp;Jonathan Ononiwu ,&nbsp;Tyler J. Avis","doi":"10.1016/j.bbrc.2025.151651","DOIUrl":"10.1016/j.bbrc.2025.151651","url":null,"abstract":"<div><div>The stability of the plasma membrane is crucial for cell viability and disruptions in membrane stability can significantly impact cell function. Antimicrobial compounds targeting fungal membranes are required as novel alternatives to current resistance-prone fungicides. Six antimicrobials were assessed using the yeast <em>Saccharomyces cerevisiae</em> in living and artificial membrane models to gain insight into their efficacy and mechanistic activity. Antimicrobial-treated yeast cultures were monitored for growth inhibition and cell membrane permeability. Liposomes prepared from yeast polar lipids were used to examine the impact of the antimicrobials on size, polydispersity, and ζ-potential. Iturin and nystatin were the most effective compounds in reducing growth and increasing membrane permeability. ζ-Potential measurements indicated that iturin caused reduced stability, whereas there were no changes in stability with nystatin. Daptomycin and fengycin did not affect growth or permeability, but reduced stability. Nisin inhibited growth but did not affect stability. Surfactin was the only tested compound to increase stability. Results indicate that antimicrobials known to target biomembranes had variable effects, with lipid membrane components playing a role in antifungal outcome and mechanistic activity.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151651"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Main methods and tools for peptide development based on protein-protein interactions (PPIs)","authors":"Javiera Baeza ,&nbsp;Mauricio Bedoya ,&nbsp;Pablo Cruz ,&nbsp;Paola Ojeda ,&nbsp;Francisco Adasme-Carreño ,&nbsp;Oscar Cerda ,&nbsp;Wendy González","doi":"10.1016/j.bbrc.2025.151623","DOIUrl":"10.1016/j.bbrc.2025.151623","url":null,"abstract":"<div><div>Protein-protein interactions (PPIs) regulate essential physiological and pathological processes. Due to their large and shallow binding surfaces, PPIs are often considered challenging drug targets for small molecules. Peptides offer a viable alternative, as they can bind these targets, acting as regulators or mimicking interaction partners. This review focuses on competitive peptides, a class of orthosteric modulators that disrupt PPI formation. We provide a concise yet comprehensive overview of recent advancements in <em>in-silico</em> peptide design, highlighting computational strategies that have improved the efficiency and accuracy of PPI-targeting peptides. Additionally, we examine cutting-edge experimental methods for evaluating PPI-based peptides. By exploring the interplay between computational design and experimental validation, this review presents a structured framework for developing effective peptide therapeutics targeting PPIs in various diseases.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151623"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}