Biochemical and biophysical research communications最新文献

{"title":"Testosterone suppression with or without testicular Preservation: Impact on mouse behavior, including emotional state, locomotor activity, social interaction, cognitive function, and sexual behavior","authors":"Yaxin Zang ,&nbsp;Liuxia Lin ,&nbsp;Yigeng Lu ,&nbsp;Mengzhen Luo ,&nbsp;Limin Wang ,&nbsp;Mengsi Xu ,&nbsp;Amei Huang ,&nbsp;Jian Ma ,&nbsp;Chunmei Du ,&nbsp;Chen Wei ,&nbsp;Wujun Liu ,&nbsp;Rui Gao ,&nbsp;Shangquan Gan","doi":"10.1016/j.bbrc.2025.152187","DOIUrl":"10.1016/j.bbrc.2025.152187","url":null,"abstract":"<div><div>The deposition of androgens significantly influences livestock meat quality, yet traditional surgical castration methods involving testicular removal raise concerns about animal welfare due to their association with stress and behavioral changes. To address these issues, this study compared two approaches—surgical castration (testicular removal) and immunocastration (retention of testicular tissue)—to evaluate their psychological and behavioral impacts in mice across domains such as sexual behavior, emotional regulation, locomotor activity, social interaction, and memory performance. Results demonstrated that surgical castration induced robust depressive-like symptoms, including reduced motivation and impaired social engagement, whereas immunocastrated mice exhibited normal emotional and social behaviors without signs of depression or distress. These findings highlight the psychological burden of surgical castration and underscore the ethical implications of its use. Immunocastration emerges as a more humane alternative, effectively controlling sexual behavior without inducing depressive-like symptoms, likely by preserving the animals' sense of normalcy and dignity. This study emphasizes the importance of balancing productivity with ethical considerations in livestock management and provides valuable insights for advancing welfare-friendly castration methods. By advocating for immunocastration, this research contributes to improving animal welfare standards while maintaining meat quality, offering a promising alternative to traditional practices.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152187"},"PeriodicalIF":2.5,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shp2 regulates the trophoblast cell cycle progression through p53-p21 pathway modulation","authors":"Ming-hui Meng ,&nbsp;Xin Wu ,&nbsp;Ting Qin ,&nbsp;Wei-ying Mo ,&nbsp;Xi-gui Long ,&nbsp;Ying Li ,&nbsp;Peng Zhang ,&nbsp;Jian-ning Lu ,&nbsp;Dongjin Pan ,&nbsp;Xiu-qun Zhang","doi":"10.1016/j.bbrc.2025.152209","DOIUrl":"10.1016/j.bbrc.2025.152209","url":null,"abstract":"<div><div>Normal trophoblast cells proliferation and migration are crucial for placental development. Dysfunction in these processes is closely linked to pregnancy-related diseases, such as preeclampsia (PE) and fetal growth restriction (FGR). This study aimed to explore the role of Src homology 2 domain-containing protein tyrosine phosphatase 2 (Shp2) in regulating trophoblast cell HTR-8/SVneo (HTR8) functions and its underlying mechanisms. By using a specific Shp2 inhibitor (SHP099) and lentivirus-mediated Shp2 knockdown combined with transcriptome sequencing, we found that Shp2 inactivation significantly inhibited HTR8 proliferation by inducing G₀/G₁ cell cycle arrest and reduced migratory/invasive capacities. The transcriptomic study revealed that Shp2 knockdown in HTR8 cells significantly upregulated p53 pathway downstream genes (<em>CDKN1A</em>, <em>MDM2, etc</em>.). Western blot results showed that Shp2 downregulation increased p21 protein levels, suggesting that Shp2 probably maintains cell cycle progression by suppressing the p53-p21 axis. Both pharmacological inhibition and genetic knockdown of Shp2 modulated Erk1/2 and Akt activities, indicating its roles in trophoblast functions through MAPK and PI3K-Akt signaling. This study provides new insights into the molecular mechanisms governing trophoblast biology and potential therapeutic targets for placental disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152209"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early transcriptional divergence in mRNA vaccines reveals molecular correlates of humoral efficacy","authors":"Qian Wang ,&nbsp;Ziyang Song ,&nbsp;Jialu Zhang ,&nbsp;Lifang Song ,&nbsp;Dong Liu ,&nbsp;Xing Wu ,&nbsp;Fan Gao ,&nbsp;Mingchen Liu ,&nbsp;Lu Li ,&nbsp;Ying Wang ,&nbsp;Qian He ,&nbsp;Xuanxuan Zhang ,&nbsp;Qunying Mao ,&nbsp;Zhenglun Liang","doi":"10.1016/j.bbrc.2025.152199","DOIUrl":"10.1016/j.bbrc.2025.152199","url":null,"abstract":"<div><div>Although immune responses had been characterized for individual mRNA vaccine, comprehensive analyses of shared features remain limited. To address this, we employed transcriptomic analysis to investigate immune response patterns of three mRNA vaccines in mice, revealing shared responses to mRNA vaccine. Our analysis indicated that vaccines inducing higher antibody titers significantly upregulated pathways including complement cascades, endosomal/vacuolar trafficking, neutrophil degranulation, molecular chaperones, complement pathways, MHC class II antigen presentation, and cell cycle pathways. Unique differently expressed genes (DEGs) in high-response vaccines were identified as potential correlates of humoral efficacy. Totally, our study provides insights into the development and evaluation of mRNA vaccines.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152199"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of ergothioneine distribution across skeletal muscles and adipose tissues","authors":"Kentaro Nishioka,&nbsp;Takahiro Ishimoto,&nbsp;Makoto Katsube,&nbsp;Shoma Yamada,&nbsp;Yudai Araragi,&nbsp;Reiya Yamashita,&nbsp;Yukio Kato","doi":"10.1016/j.bbrc.2025.152210","DOIUrl":"10.1016/j.bbrc.2025.152210","url":null,"abstract":"<div><div>Ergothioneine (EGT), a food-derived amino acid, is distributed to various organs via the carnitine/organic cation transporter, OCTN1. However, the distribution characteristics of EGT across different skeletal muscles and adipose tissues remains unexplored. This study evaluated the distribution of EGT and OCTN1 mRNA expression in the soleus, gastrocnemius, plantaris muscle, epididymal white adipose tissue (eWAT), and interscapular brown adipose tissue (iBAT) of middle-aged C57BL/6 J wild-type male mice, which were orally administered 20 mg/kg of EGT once daily for up to 56 days. EGT was distributed at significantly higher concentrations in the soleus than in the gastrocnemius and plantaris muscles. Similarly, the mRNA level of OCTN1 was significantly higher in the soleus muscle. Regarding adipose tissue, EGT was distributed at significantly higher concentrations in iBAT than in eWAT. The soleus and iBAT may have a higher risk of oxidative stress, which could be a probable reason for the higher distribution of the antioxidant EGT. This study revealed the unique characteristics of EGT distribution in skeletal muscles and adipose tissues, providing a solid foundation for future studies on EGT health strategies.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152210"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IRF3-p300 axis controls global acetylation and mitotic progression","authors":"Won-Joo Kim ,&nbsp;Abdul Basit ,&nbsp;Eun-Bi Ko ,&nbsp;Yungyeong Heo ,&nbsp;Jaehyun Shim ,&nbsp;Su-Bin Lim ,&nbsp;Jae-Ho Lee","doi":"10.1016/j.bbrc.2025.152211","DOIUrl":"10.1016/j.bbrc.2025.152211","url":null,"abstract":"<div><div>Accurate mitotic progression depends on precisely regulated post-translational modifications, including lysine acetylation, but the upstream mechanisms governing acetylation during mitosis remain unclear. Here, we identify the IRF3–p300 axis as a major regulator of global protein acetylation during mitosis. We show that p300 serves as the major lysine acetyltransferase active during mitosis, and its enzymatic activity is essential for proper cell division. Notably, p300 activation during mitosis requires phosphorylation and dimerization of IRF3, a transcription factor known for its role in innate immunity. IRF3 interacts with and activates p300, and depletion of either IRF3 or p300 reduces global mitotic protein acetylation and delays mitotic progression. These defects are rescued by wild-type IRF3 or p300, but not by phosphorylation- or dimerization-deficient IRF3 mutants or catalytically inactive p300. Mass spectrometry analysis reveals that the mitotic acetylome is substantially altered upon loss of IRF3 or p300, with widely overlapping subsets of non-histone proteins—many involved in RNA biogenesis and processing—being affected. These findings reveal a non-canonical role for IRF3 as an upstream activator of p300 and establish the IRF3–p300 axis as a key signaling pathway that ensures proper mitotic progression through global regulation of protein acetylation.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152211"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of high SAP2 expression on the invasion and adhesion abilities of Candida albicans in vaginal epithelial cells","authors":"Lan Xue,&nbsp;Lu Yang,&nbsp;Xize Fu,&nbsp;Wenli Feng,&nbsp;Jing Yang,&nbsp;Yan Ma,&nbsp;Zhiqin Xi","doi":"10.1016/j.bbrc.2025.152147","DOIUrl":"10.1016/j.bbrc.2025.152147","url":null,"abstract":"<div><h3>Background</h3><div>The link between <em>SAP2</em> and <em>Candida albicans</em>' capacity to adhere to and invade host cells is notable, although the precise molecular mechanisms remain unclear. The purpose of this study was to examine how elevated <em>SAP2</em> expression influences the invasion and adhesion capabilities of <em>C. albicans</em> in A431 cells using in vitro experiments and transcriptomic sequencing.</div></div><div><h3>Methods</h3><div>The effect of high <em>SAP2</em> expression on the invasion of A431 cells by <em>C. albicans</em> was evaluated using lactate dehydrogenase (LDH) release assay. Morphological changes in hyphae were observed under a microscope during co-culture with A431 cells. The ability of <em>C. albicans</em> to adhere to A431 cells was assessed using an adhesion assay. Transcriptomic sequencing and qRT-PCR were used to examine changes in gene expression and associated signaling pathways in <em>C. albicans</em> after co-culture with A431 cells.</div></div><div><h3>Results</h3><div>After 1.5–2 h of co-culture, the number of A431 cell colonies adhered by the SC5314 strain was significantly higher than adhered by the <em>SAP2</em>-overexpressing SC5314 strain (pRB895-<em>SAP2</em>-SC5314). After 8 h, the SC5314 strain induced the formation of dense, elongated hyphae, whereas the pRB895-<em>SAP2</em>-SC5314 strain formed clustered, snowflake-like yeast forms. After 24 h, the LDH release assay revealed a significantly higher death rate of A431 cells in the SC5314 group. Differential expression analysis identified 50 upregulated and 10 downregulated genes in the pRB895-SC5314 strain relative to the SC5314 strain. A total of 1142 genes were upregulated and 1219 genes were downregulated in the pRB895-<em>SAP2</em>-SC5314 strain relative to the SC5314 strain. In addition, 1159 genes were upregulated and 1228 genes were downregulated in the pRB895-<em>SAP2</em>-SC5314 strain relative to the pRB895-SC5314 strain. KEGG enrichment analysis indicated the MAPK signaling pathway associated with these genes, and qRT-PCR showed significant downregulation of genes such as <em>TEC1</em>.</div></div><div><h3>Conclusion</h3><div>Co-culture with A431 cells significantly inhibited hyphal growth and attenuated the invasion and adhesion abilities of the pRB895-<em>SAP2</em>-SC5314 strain. Several genes related to <em>SAP2</em> were upregulated or downregulated, suggesting that the role of <em>SAP2</em> in the pathogenic mechanisms of <em>C. albicans</em> is influenced by complex regulatory factors, with the MAPK signaling pathway serving as a key factor.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152147"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conditional knockout mouse model demonstrates that Copa expression is required for viability in development and adulthood","authors":"Timra Gilson ,&nbsp;Jacob W. Astroski ,&nbsp;Wang Qun ,&nbsp;Matthew J. Turner ,&nbsp;Elliot J. Androphy ,&nbsp;Sara K. Custer","doi":"10.1016/j.bbrc.2025.152201","DOIUrl":"10.1016/j.bbrc.2025.152201","url":null,"abstract":"<div><div>The COPI coatomer is a heptameric complex that regulates traffic between the Golgi apparatus and the Endoplasmic Reticulum (ER). Mutations in the <em>COPA</em> gene encoding the alpha subunit of the COPI complex result in an autoimmune disorder impacting multiple tissues including the lungs, joints, and kidneys. We report here the characterization of a conditional-ready <em>Copa</em> knockout mouse. Systemic homozygous deletion of <em>Copa</em> in mice resulted in embryonic lethality. Deletion of <em>Copa</em> in Chat-positive cells also resulted in late embryonic lethality. However, the deletion of <em>Copa</em> by Cre recombinase expression under the Mnx1 promoter was compatible with development and viability. In adulthood, these mice display a modest glucose intolerance, most likely due to Mnx1-driven pancreatic expression of Cre recombinase. Systemic deletion of <em>Copa</em> in adult mice results in rapid decline and death. Lung fibroblasts cultured from mice expressing a Tamoxifen-inducible Cre recombinase demonstrate robust <em>Copa</em> knockout, resulting in near complete loss of alpha-COP protein. These cultures recapitulate the previously described COPA syndrome phenotype, including hyperactivation of STING (Stimulator of Interferon Genes) signaling and induction of ER stress. Dorsal Root Ganglion neurons cultured from Tamoxifen-inducible <em>Copa</em> knockout mice show that axonal arbor maintenance and viability requires <em>Copa</em> expression.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152201"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen deficiency impairs double-negative T cell function and activates NF-κB in postmenopausal osteoporosis","authors":"Hua Jin ,&nbsp;Mengmeng Chen ,&nbsp;Xiyu Wang ,&nbsp;Guangyong Sun ,&nbsp;Hao Chen ,&nbsp;Dong Zhang","doi":"10.1016/j.bbrc.2025.152212","DOIUrl":"10.1016/j.bbrc.2025.152212","url":null,"abstract":"<div><div>Postmenopausal osteoporosis (PMOP), which is driven primarily by estrogen deficiency, is characterized by excessive bone resorption and disrupted immune homeostasis. Although immune system contributions to bone loss are well-documented, the roles of specific regulatory subsets, such as double-negative T (DNT) cells (CD3⁺TCRαβ⁺CD4⁻CD8⁻NK1.1⁻), remain unclear. Herein, by using an ovariectomized (OVX) mouse model of PMOP, we integrated transcriptomic profiling, <em>in vivo</em> functional assays, and <em>in vitro</em> studies to investigate DNT cell alterations. The OVX mice presented significant reductions in both DNT cell frequency and immunoregulatory capacity, which were accompanied by their increased apoptosis and suppressed proliferation. RNA sequencing revealed concurrent upregulation of NF-κB signaling and apoptotic pathways with downregulation of estrogen receptor (ER) signaling and cytotoxicity. <em>In vitro</em>, estrogen stimulation enhanced DNT cell immunoregulatory function and estrogen receptor <em>Esr1</em> expression, while inhibiting NF-κB activation. These results demonstrated that estrogen deficiency impaired DNT cell viability and function, potentially through NF-κB pathway activation, thereby exacerbating immune dysregulation in PMOP. Our study suggests that DNT cells may represent important immune players involved in osteoimmunology and potential targets for immunomodulatory therapies of osteoporosis.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152212"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical stimulation induces electrical coupling via TNTs through calcium-dependent mechanisms in C2C12 cells","authors":"Yanli Sun ,&nbsp;Hucheng Zhao","doi":"10.1016/j.bbrc.2025.152186","DOIUrl":"10.1016/j.bbrc.2025.152186","url":null,"abstract":"<div><div>Intercellular communication and cellular material exchange are essential for the development, tissue repair, and survival of multicellular organisms. Tunneling nanotubes (TNTs) serve as long-distance intercellular connections that facilitate the transmission of biochemical and electrical signals between cells. However, the mechanisms underlying this process remain largely unknown. This study uses mechanical stimulation to explore the transmission and mechanisms of mechano-electrical signals mediated by TNTs. We assessed both gap junction (GJ)-mediated and TNT-mediated intercellular electrical coupling in HEK cells, A549 cells, and C2C12 cells. Our results show that the coupling ratio was lower in HEK cells compared to A549 cells, with the highest ratio observed in C2C12 cells. Additionally, the strength of TNT-mediated electrical coupling decreased as TNT length increased. Using C2C12 cells as a model, we found that Ca²⁺ influx and the gap junction protein Connexin 43 (Cx43) play roles in TNT-mediated electrical coupling induced by mechanical stimulation. Mechanical stimulation triggers extracellular Ca²⁺ entry into C2C12 cells via mechanosensitive and T-type calcium channels. Our experiments also demonstrate that TNT-mediated electrical coupling between C2C12 cells is predominantly unidirectional. These findings provide new insights into the mechanisms of intercellular electrical signal transmission.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152186"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the transcriptomic characteristic of lactate metabolism and the immune infiltration landscape in abdominal aortic aneurysm","authors":"Min Zhang ,&nbsp;Ru Ying ,&nbsp;Song Lu","doi":"10.1016/j.bbrc.2025.152198","DOIUrl":"10.1016/j.bbrc.2025.152198","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Abdominal aortic aneurysm (AAA) is a common degenerative vascular disease characterized by progressive dilation of the abdominal aorta, which poses a life-threatening risk upon rupture. Lactate, a key metabolic byproduct and immunomodulatory molecule, plays a crucial role in regulating immune cell functions in various inflammatory diseases. However, the specific involvement of lactate metabolism in the pathogenesis of AAA remains poorly understood. This study aims to identify lactate metabolism-related gene signatures associated with AAA and to elucidate their potential roles and underlying mechanisms in disease progression.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Transcriptomic datasets GSE57691, GSE183464, and GSE237230 were obtained from the Gene Expression Omnibus (GEO) database. Lactate metabolism-related genes were retrieved from the Molecular Signatures Database (MSigDB). Weighted Gene Co-expression Network Analysis (WGCNA) and the Limma R package were employed to identify key gene modules associated with AAA and detect differentially expressed genes (DEGs) between AAA and control groups, respectively. Overlapping genes were subjected to functional enrichment analysis and protein–protein interaction (PPI) network construction. Three distinct machine learning algorithms were applied to screen for potential diagnostic biomarkers. Upon validation, a nomogram was constructed based on the selected biomarkers. Immune infiltration and single-cell RNA analysis were performed to characterize the immune microenvironment and investigate the association between immune cell subsets and AAA-related biomarkers. Finally, the expression patterns of the identified biomarkers were validated using a murine model of AAA.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;A total of 3336 AAA-related module genes, 2651 DEGs between AAA and controls, and 364 lactate metabolism-related genes were identified. Among these, 29 genes were recognized as lactate metabolism-related DEGs associated with AAA. Functional enrichment analysis revealed significant enrichment in pathways related to oxidative phosphorylation and energy metabolism. SLC25A4, HBB, and STAT4 were identified as candidate biomarkers for AAA. Immune infiltration analysis revealed distinct immune profiles between AAA and control groups. Single-cell mRNA analysis demonstrated that SLC25A4 is predominantly expressed in adventitial cells and fibroblasts in AAA, HBB is expressed across multiple immune cell subsets, and STAT4 is mainly expressed in T cells. Gene Set Enrichment Analysis indicated that these biomarkers are involved in biological processes related to T cell activation and T cell differentiation. These findings were further validated in a murine model of AAA.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;The identification of lactate metabolism-related biomarkers and the comprehensive characterization of the immune microenvironment in AAA offer novel insights that may contribute to the de","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"776 ","pages":"Article 152198"},"PeriodicalIF":2.5,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}