Early transcriptional divergence in mRNA vaccines reveals molecular correlates of humoral efficacy

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-06-14 DOI:10.1016/j.bbrc.2025.152199

Qian Wang , Ziyang Song , Jialu Zhang , Lifang Song , Dong Liu , Xing Wu , Fan Gao , Mingchen Liu , Lu Li , Ying Wang , Qian He , Xuanxuan Zhang , Qunying Mao , Zhenglun Liang

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引用次数: 0

Abstract

Although immune responses had been characterized for individual mRNA vaccine, comprehensive analyses of shared features remain limited. To address this, we employed transcriptomic analysis to investigate immune response patterns of three mRNA vaccines in mice, revealing shared responses to mRNA vaccine. Our analysis indicated that vaccines inducing higher antibody titers significantly upregulated pathways including complement cascades, endosomal/vacuolar trafficking, neutrophil degranulation, molecular chaperones, complement pathways, MHC class II antigen presentation, and cell cycle pathways. Unique differently expressed genes (DEGs) in high-response vaccines were identified as potential correlates of humoral efficacy. Totally, our study provides insights into the development and evaluation of mRNA vaccines.

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mRNA疫苗的早期转录分化揭示了体液疗效的分子相关性

尽管已对单个mRNA疫苗的免疫反应进行了表征，但对共同特征的综合分析仍然有限。为了解决这个问题，我们采用转录组学分析研究了三种mRNA疫苗在小鼠中的免疫反应模式，揭示了对mRNA疫苗的共同反应。我们的分析表明，诱导更高抗体滴度的疫苗显著上调了补体级联、内体/空泡运输、中性粒细胞脱颗粒、分子伴侣、补体途径、MHC II类抗原呈递和细胞周期途径。高反应疫苗中独特的不同表达基因（DEGs）被确定为体液疗效的潜在相关因素。总之，我们的研究为mRNA疫苗的开发和评价提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics