Biochemical and biophysical research communications最新文献

{"title":"Co-medication effects of Doxorubicin and Bendamustine on DNA molecules ananlyized with a variety of single-molecule spectroscopy","authors":"Qing He ,&nbsp;Remila Abulaiti ,&nbsp;Xiaoqiang Feng ,&nbsp;Lu Tian ,&nbsp;Kaige Wang","doi":"10.1016/j.bbrc.2025.152275","DOIUrl":"10.1016/j.bbrc.2025.152275","url":null,"abstract":"<div><div>Combination drug therapy is an important strategy to reduce the side effects and enhance the positive effects of chemotherapy drugs. Doxorubicin (DOX) and Bendamustine (BENDA) are two widely used and effective chemotherapeutic agents for treating tumors by targeting DNA molecules, and recent clinical data have displayed that their combination therapy is efficacious; However, no systematic study has clarified the synergistic or competitive binding mechanism of these two drugs to DNA at the molecular level. In this study, cooperated with the surface-enhanced Raman spectroscopy, UV–Vis spectroscopy and molecular docking simulation, we systematically investigated the combined effects of DOX and BENDA on ctDNA at the single-molecule level. The results show that DOX primarily binds to the N7 site of adenine via hydrogen bonding, while BENDA interacts with the N3 site of the same adenine molecule, demonstrating a synergistic effect. In contrast, both DOX and BENDA compete for binding to the N3 and NH₂ sites of guanine, showing a competitive relationship. Moreover, the binding behavior is significantly influenced by the sequence of drug administration: when DOX is added before BENDA, BENDA can partially replace the previously bound DOX at its binding sites, and vice versa. Gel electrophoresis experiments showed that BENDA can induce DNA strand breaks, and this effect can be partially inhibited by DOX pre-bound to DNA. This study, for the first time, reveals the synergistic and competitive interaction mechanisms of DOX and BENDA on DNA at the single-molecule level, providing theoretical support and experimental evidence for optimizing combination therapies and designing low-toxicity, high-efficacy anticancer drugs.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152275"},"PeriodicalIF":2.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microperforations promote Notch-2 expression to accelerate orthodontic tooth movement","authors":"Yao Jiao ,&nbsp;Xiaoyan Li ,&nbsp;Siyan Liu ,&nbsp;Sicong Mi ,&nbsp;Wenrui Han ,&nbsp;Juan Du ,&nbsp;Junji Xu ,&nbsp;Lijia Guo ,&nbsp;Song Li ,&nbsp;Yi Liu ,&nbsp;Yitong Liu","doi":"10.1016/j.bbrc.2025.152267","DOIUrl":"10.1016/j.bbrc.2025.152267","url":null,"abstract":"<div><div>Microperforations (MOPs), as one of the periodontal procedures, can accelerate the rate of orthodontic tooth movement. However, the molecular mechanism remains to be clarified. This study aims to investigate the effect of the surgical microenvironment on bone marrow-derived monocytes (BMMNCs) and provide a theoretical basis for exploring the mechanisms through which MOPs accelerate orthodontic tooth movement. An animal model of accelerated orthodontics induced by MOPs was established by drilling around the first molar of rats. Stereoscopic microscopy, micro-CT, H&amp;E staining, and TRAP staining were utilized to observe tooth movement, periodontal tissue, and the number of osteoclasts post-surgery. RT-PCR, Western blotting, and TRAP staining were conducted to examine the impact of the surgery-induced microenvironment on the osteoclast differentiation ability of monocytes. Compared to general orthodontics, a cytokine array was employed to detect expression changes of tissue proteins in MOPs-accelerated orthodontics. The cytokine array revealed that the expression of Notch-2 increased in alveolar bone tissue after MOPs. <em>In vitro</em> experiments confirmed the significant role of Notch-2 in the MOPs + TM group using small interfering RNA (siRNA) technology. This is also linked to the activation of the NF-κB pathway. These findings suggest that MOPs can enhance the expression of Notch-2 in the tissues, activate the NF-κB pathway of monocytes, promote their osteoclast differentiation, and accelerate tooth movement.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152267"},"PeriodicalIF":2.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural insights into monoterpene cyclisation of limonene synthase from Cannabis sativa","authors":"Danielle Wiles ,&nbsp;James Roest ,&nbsp;Julian P. Vivian ,&nbsp;Travis Beddoe","doi":"10.1016/j.bbrc.2025.152271","DOIUrl":"10.1016/j.bbrc.2025.152271","url":null,"abstract":"<div><div>Terpenes are the largest and most diverse class of natural products, essential for plant defence, ecological interactions, and environmental adaptation. <em>Cannabis sativa</em> is noted for its rich terpene profile, influencing aroma, flavour, and pharmacological properties. Limonene, a significant monoterpene, is commercially important in the fragrance and flavouring industries, making it a target for metabolic engineering. Terpene biosynthesis involves terpene synthase enzymes that convert isoprenoid diphosphates into diverse terpene scaffolds. Despite advances in terpene biochemistry, <em>C. sativa</em> TPSs lack structural characterisation. This study presents the first crystal structure of (−)-limonene synthase from <em>C. sativa</em>, offering insights into monoterpene biosynthesis. Solved at 3.2 Å resolution, the structure shows an “open” conformation with a solvent-accessible active site and disordered loops near the catalytic pocket, indicating a pre-catalytic state that aids substrate access. Biochemical characterisation confirmed limonene synthase as a highly specific monoterpene synthase, predominantly producing (−)-limonene from geranyl diphosphate with minor amounts of eight other monoterpenes. Kinetic analysis provided a K_m of 7.809 ± 0.678 μM and a k_cat of 0.0204 s⁻¹, indicating moderate catalytic efficiency compared to other plant monoterpene synthases. These findings improve understanding of TPS function and set the stage for enzyme engineering to optimise terpene biosynthesis for industrial and biotechnological applications.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152271"},"PeriodicalIF":2.5,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA HOTAIR regulates radio-resistance in squamous cell carcinoma of the tongue by Notch signaling","authors":"Yang Zhang ,&nbsp;Chen-xi Li ,&nbsp;Chong Guo ,&nbsp;Pan Liu ,&nbsp;Cheng Li ,&nbsp;Hua-rong Zhao ,&nbsp;Zhong-cheng Gong","doi":"10.1016/j.bbrc.2025.152258","DOIUrl":"10.1016/j.bbrc.2025.152258","url":null,"abstract":"<div><h3>Objective</h3><div>To minimize local recurrence of tongue cancer, it is necessary to tackle the issues of radiotherapy resistance and sensitivity in tongue squamous cell carcinoma (TSCCa). This study focuses on understanding how HOX transcript antisense intergenic RNA (HOTAIR) influences resistance to radiotherapy in TSCCa by modulating the Notch signaling pathway.</div></div><div><h3>Methods</h3><div>The TSCCa cells SCC9 and SCC25 were divided into six experimental groups: (1) 8 Gy irradiation group, (2) 8 Gy with negative control, (3) 8 Gy with silent HOTAIR, (4) 8 Gy with null control, (5) 8 Gy with overexpressing HOTAIR, and (6) 8 Gy with silent HOTAIR and Jagged1. After assessing changes in apoptosis, proliferation, and invasion abilities of cells in each group using CCK-8, flow cytometry, and Transwell assays, we also utilized qRT-PCR and WesternBlot to evaluate changes in genes and proteins associated with the Notch pathway. These alterations induced by HOTAIR were validated in vivo using nude mouse tumor-bearing model.</div></div><div><h3>Results</h3><div>In the silenced HOTAIR group, both ex vivo and in vivo studies revealed decreased cell survival and invasiveness, increased apoptosis, and significantly reduced expression of Notch1, Jagged1, and HES-1 at gene and protein levels (all <em>P</em> &lt; 0.05). In contrast, adding an agonist of the Notch signaling pathway produced opposite results (all <em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>The regulation of the Notch signaling pathway by HOTAIR is associated with resistance to radiotherapy in squamous cell carcinoma of the tongue.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152258"},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptochromes induce genome-wide changes in alternative transcription start site selection in arabidopsis","authors":"Yoshito Oka ,&nbsp;Kazumasa Shirai ,&nbsp;Izumi Kimura ,&nbsp;Tomoo Shimada ,&nbsp;Kousuke Hanada ,&nbsp;Tomonao Matsushita","doi":"10.1016/j.bbrc.2025.152264","DOIUrl":"10.1016/j.bbrc.2025.152264","url":null,"abstract":"<div><div>Cryptochromes (CRYs) are blue-light photoreceptors that regulate diverse physiological responses in plants. CRYs intervene the transcript level of numerous genes by modulating specific transcription factors, including ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOGUE (HYH). In this study, we conducted a genome-wide analysis to profile blue light-induced changes in alternative transcription start site (TSS) selection in Arabidopsis. We identified 3587 genes exhibiting CRY-dependent and blue light-responsive changes in alternative TSS usage, among which 2197 were predicted to produce multiple protein isoforms with different N-terminal ends through this mechanism. These findings suggest that CRYs contribute to proteome complexity through the control of alternative TSS selection. Furthermore, mutants lacking both <em>HY5</em> and <em>HYH</em> displayed abnormal blue light-induced changes in alternative TSS selection for a subset of CRY target genes, implicating these transcription factors as downstream components in this regulatory pathway. Our results uncover a previously unrecognized function of CRYs in controlling qualitative aspects of transcriptome via blue light-dependent alternative TSS selection.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152264"},"PeriodicalIF":2.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Mg-independent and Mg-dependent Ecto-ATPase activities in luminal a breast cancer MCF-7 cells","authors":"Marco Antonio Lacerda-Abreu ,&nbsp;Enderson Silva ,&nbsp;Bruna dos Santos Mendonça ,&nbsp;Gabriela Nestal de Moraes ,&nbsp;José Roberto Meyer-Fernandes","doi":"10.1016/j.bbrc.2025.152256","DOIUrl":"10.1016/j.bbrc.2025.152256","url":null,"abstract":"<div><div>Breast cancer remains the most prevalent malignancy among women, characterized by molecular heterogeneity and complex tumor microenvironment interactions. Purinergic signaling, particularly through extracellular adenosine triphosphate (ATP) hydrolysis, plays a significant role in regulating cancer progression. Ectonucleotidases and ectophosphatases modulate ATP and inorganic phosphate (Pi) metabolism, thereby influencing tumor-associated processes such as migration and adhesion. In this study, we biochemically characterized ecto-ATPase activity in luminal breast cancer cells (MCF-7) and compared it to non-tumorigenic breast epithelial cells (MCF10-A). We identified distinct Mg²⁺-dependent and independent ecto-ATPase activities, with MCF-7 cells exhibiting a significant Mg²⁺-dependent increase in ATP hydrolysis. Kinetic analyses revealed that Mg²⁺-independent activity presents higher substrate affinity, whereas the Mg²⁺-dependent enzyme displays greater catalytic capacity. Substrate specificity assays demonstrated preferential hydrolysis of ATP and ADP, highlighting the potential role of ecto-ATPase in extracellular nucleotide homeostasis. Pharmacological inhibition with ARL67156 reduced Mg²⁺-dependent ecto-ATPase activity, leading to a significant decrease in cell migration and adhesion, indicating a functional role in breast cancer progression. These findings underscore the relevance of ecto-ATPase in luminal breast cancer and its potential as a therapeutic target. Future investigations should explore its interplay with purinergic signaling and oncogenic pathways to develop novel strategies for targeted breast cancer treatment.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152256"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of benfotiamine supplementation and aerobic training against noise-induced cardiovascular damage: A focus on oxidative stress and inflammatory pathways","authors":"Hadiseh Abdi Ziari ,&nbsp;Fatemeh Shaki ,&nbsp;Mohammad Seyedabadi ,&nbsp;Alireza Safarzade ,&nbsp;Fereshteh Talebpour Amiri ,&nbsp;Elahe Talebi-Garakani ,&nbsp;Mohammad Shokati Sayyad","doi":"10.1016/j.bbrc.2025.152235","DOIUrl":"10.1016/j.bbrc.2025.152235","url":null,"abstract":"<div><h3>Background</h3><div>Environmental pollution, particularly noise exposure, may contribute to the development and progression of cardiovascular disorders by triggering oxidative stress and inflammatory pathways. This study evaluated the protective effects of benfotiamine (BFT) supplementation and moderate-intensity continuous training (MICT), alone or in combination, against noise-induced cardiac damage in male mice.</div></div><div><h3>Methods and results</h3><div>Eight-week-old mice (n = 8/group) were divided into six groups: control, noise-exposed (Noise) subjected to 100 dB (dB), noise + moderate-intensity continuous exercise training (MICT), noise + BFT group (200 mg/kg/day), noise + MICT + BFT, and noise + N‐acetylcysteine (NAC) groups. Noise exposure and other treatments were administered over four weeks. Histopathological changes, oxidative stress parameters, and the gene expression of inflammatory markers were evaluated.</div><div>Noise exposure markedly increased cardiac ROS, NO, MDA, and protein carbonyl content, while significantly decreasing GSH and FRAP levels (all p &lt; 0.001 vs. control). Treatment with BFT or MICT partially restored redox balance, whereas combined BFT + MICT treatment produced more pronounced improvements (e.g., MDA: 9.91 ± 4.45; GSH: 101.2 ± 20.1 μM). Inflammatory markers IL-6, TNF-α, IL-1β, and NF-κB were upregulated by noise and significantly attenuated by all interventions, with the greatest reduction observed in the combined group. Histological analysis confirmed that the combined therapy more effectively preserved myocardial architecture compared to monotherapies.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that BFT's antioxidant and anti-inflammatory properties, in combination with MICT as a non-pharmacological approach, may protect against noise-induced cardiovascular problems. BFT and MICT mitigate noise-induced cardiac injury via antioxidant and anti-inflammatory mechanisms, with additive benefits evident in the combined treatment group.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152235"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of neuroprotective effects of newly synthesized benzimidazolium salt against neurotoxicity in differentiated SH-SY5Y neuronal cells","authors":"Fatma Yıldız","doi":"10.1016/j.bbrc.2025.152255","DOIUrl":"10.1016/j.bbrc.2025.152255","url":null,"abstract":"<div><div>Neurodegenerative diseases (ND) are a group of neurological disorders characterized by various pathological features such as selective neuronal loss, chronic inflammation, and aggregation of specific proteins. This study aimed to investigate the possible protective effects of newly synthesized benzimidazolium salt (BS) against H₂O₂-induced oxidative stress in differentiated SH-SY5Y cells. Firstly, retinoic acid was applied to SH-SY5Y cells to obtain mature neurons. Then, a toxicity model was created by H₂O₂ treatment of neuron-like differentiated SH-SY5Y (d- SH-SY5Y) cells. Survival rates of d–SH–SY5Y cells were determined using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) method. In addition, pro-inflammatory cytokine (tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6) levels were determined by ELISA method, while gene expression levels of apoptotic markers (Bax, Bcl-2, caspase-3 and caspase-8) were determined by RT-QPCR method. In addition, the effect of BS on neurite length in these cell groups was evaluated through morphological observations. According to our results, BS showed neuroprotective effect by increasing cell viability against H₂O₂-induced neurotoxicity in d–SH–SY5Y cells (<em>p</em> &lt; 0.01). BS pretreatment provided H₂O₂-induced Bcl-2 up-regulation, Bax down-regulation, caspase-3 activation and caspase-8 inhibition. In addition, BS supported the decrease in TNF-α, IL-1β and IL-6 protein levels. This study demonstrated for the first time that BS exhibited potential neuroprotective effects on H₂O₂-induced neuronal damage by attenuating inflammation and apoptosis. These findings suggest that BS may be considered a promising candidate in preventing and treating oxidative stress-mediated neurodegenerative diseases.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152255"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PPARα regulates acyl-CoA oxidase 1 (ACOX1) but not catalase","authors":"Xue Chen ,&nbsp;Anna Mazur ,&nbsp;Wei Xu ,&nbsp;W. Christopher Risher ,&nbsp;Krista L. Denning ,&nbsp;Yongke Lu","doi":"10.1016/j.bbrc.2025.152247","DOIUrl":"10.1016/j.bbrc.2025.152247","url":null,"abstract":"<div><div>Peroxisomes are membranous organelles, and peroxisomal membrane protein 70 (PMP70), a peroxisome transporter, is used as a marker of peroxisomes. Like mitochondria, peroxisomes can also oxidize fatty acids and its rate limiting enzyme is acyl-CoA oxidase 1 (ACOX1). But unlike mitochondria, peroxisomes generate hydrogen peroxide (H₂O₂) by ACOX1, and the generated H₂O₂ is locally detoxified by catalase. PPARα agonist WY-14,643 induces peroxisome proliferation as indicated by the induction of PMP70, ACOX1 and catalase were also induced. However, ACOX1 was induced to a greater extent than catalase. After WY-14,643 withdrawal, the induced ACOX1 started to decrease after 2 days; but catalase remained at higher levels up to10 days. While mRNA levels of ACOX1 were also induced by WY-14,643, mRNA levels of catalase were not induced by WY-14,643. In the livers collected from liver-specific PEX16 knockout (<em>Pex16</em>^{<em>Alb-Cre</em>}) mice, peroxisomes are absent but catalase and ACOX1 were somehow upregulated. The mRNA of ACOX1 was also spontaneously elevated, but the catalase mRNA was not changed. When PPARα was abrogated through crossing the <em>Pex16</em>^{<em>Alb-Cre</em>} mice with <em>Pparα</em>^{<em>−/−</em>} mice to create the <em>Pparα</em>^{<em>−/−</em>}/<em>Pex16</em>^{<em>Alb-Cre</em>} mice, the upregulated ACOX1 protein was suppressed in the <em>Pparα</em>^{<em>−/−</em>}/<em>Pex16</em>^{<em>Alb-Cre</em>} mice, but catalase protein remained elevated. These results suggest that ACOX1 but not catalase is regulated by PPARα.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152247"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotonin regulates nutrient-dependent food intake in Drosophila","authors":"Tae Hoon Ryu ,&nbsp;Kweon Yu","doi":"10.1016/j.bbrc.2025.152250","DOIUrl":"10.1016/j.bbrc.2025.152250","url":null,"abstract":"<div><div>Serotonin is a key neurotransmitter in feeding behavior. However, its role in regulating food intake based on nutrient composition remains unclear. In this study, we utilized the Drosophila model to investigate the influence of serotonin on feeding behavior. By employing transient thermogenetic tools, we transiently manipulated the activity of serotonergic neurons while minimizing effects on other physiological processes, including sleep. By measuring the intake of sucrose, sucralose, yeast, and tryptone, we evaluated the role of serotonin signaling in nutrient-dependent food intake. Our findings demonstrate that distinct subsets of serotonergic neurons in the subesophageal zone (SEZ) independently regulate the intake of sucrose and protein. Additionally, we identified serotonin receptors <em>5-HT2B</em> and <em>5-HT1A</em> in the mushroom body γ lobes as key modulators of sucrose and protein consumption, respectively. Taken together, our results highlight the role of serotonin in nutrient-specific feeding regulation and provide insights into the neural mechanisms underlying dietary choices.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"777 ","pages":"Article 152250"},"PeriodicalIF":2.5,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}