Biochemical and biophysical research communications最新文献

Affibody-based HER2 prodrug shows conditional cytotoxic effect on HER2-positive cancer cells.

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-18 DOI: 10.1016/j.bbrc.2025.151660

Cornelia Westerberg, Anna Mestre Borras, Stefan Ståhl, John Löfblom

{"title":"Affibody-based HER2 prodrug shows conditional cytotoxic effect on HER2-positive cancer cells.","authors":"Cornelia Westerberg, Anna Mestre Borras, Stefan Ståhl, John Löfblom","doi":"10.1016/j.bbrc.2025.151660","DOIUrl":"https://doi.org/10.1016/j.bbrc.2025.151660","url":null,"abstract":"<p><p>Therapeutic affinity proteins offer a targeted mode of action due to their typically high affinity and specificity for disease-associated molecules. In cancer therapy, such target molecules are often overexpressed receptors on tumor cells. However, their presence in healthy tissues can lead to on-target, off-tumor toxicity, necessitating strategies to enhance tumor selectivity. Here, we present an affibody-based prodrug concept that exploits tumor-associated proteases for selective activation. As proof of concept, we designed, produced, and characterized HER2-specific prodrug candidates, each incorporating a distinct protease substrate for selective activation by tumor-associated proteases. Their activation by corresponding proteases and subsequent HER2 binding were assessed. The most promising prodrug candidate was conjugated to the cytotoxic agent DM1 and evaluated for cytotoxicity in HER2-positive cancer cells. The results demonstrated potent, HER2-dependent cell killing, with markedly reduced cytotoxicity in the absence of prodrug activation. These findings support the feasibility of affibody-based prodrugs as a strategy to enhance tumor selectivity and minimize off-tumor toxicity in targeted cancer therapy.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"151660"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel replicase-mediated self-amplifying RNA amplification mechanism of the SARS-CoV-2 replication-transcription system.

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-18 DOI: 10.1016/j.bbrc.2025.151654

Hsien-Lin Liu, Sam Lin, William Hung, Donald C Chang, Shi-Lung Lin

{"title":"A novel replicase-mediated self-amplifying RNA amplification mechanism of the SARS-CoV-2 replication-transcription system.","authors":"Hsien-Lin Liu, Sam Lin, William Hung, Donald C Chang, Shi-Lung Lin","doi":"10.1016/j.bbrc.2025.151654","DOIUrl":"https://doi.org/10.1016/j.bbrc.2025.151654","url":null,"abstract":"<p><p>A novel self-amplifying mRNA (samRNA) amplification mechanism was first discovered in the SARS-CoV-2 replication-transcription system and named replicase cycling reaction (RCR). In principle, RCR is a replicase-mediated transcription reaction driven by the SARS-CoV-2 RNA-dependent RNA polymerases (RdRPs), which amplify a specific samRNA construct consisting of an RNA/mRNA sequence flanked by a 5'-end RdRP-reverse-promoter (5'-RdRP-RP) and a 3'-end RdRP-forward-promoter (3'-RdRP-FP) on both sides. Based on this samRNA composition, we had not only successfully established the first in-vitro RCR reaction for directly amplifying the SARS-CoV-2 genomic and subgenomic RNAs but also further used it in a combined in-vitro-transcription and RCR (IVT-RCR) protocol to identify new functions of the SARS-CoV-2 NSP7, NSP8, and NSP12 proteins, leading to a fast diagnostic assay for measuring the SARS-CoV-2 RdRP activity. These findings may shed a new light on the molecular mechanisms of SARS-CoV-2 replication and transcription. As a result, in addition to the previously found primer-dependent RNA synthesis activity of the coronaviral RdRP complexes, we herein reported another new 5'/3'-promoter-dependent, primer-independent samRNA synthesis mechanism mediated by the SARS-CoV-2 RdRP complex. Based on this novel RCR mechanism, the associated samRNA composition is conceivably useful for facilitating the design and development of next-generation RNA/mRNA medicines and vaccines.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"151654"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin mitigates heatstroke-induced myocardial injury by modulating the Akt/Bad/Caspase-3 pathway

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-17 DOI: 10.1016/j.bbrc.2025.151653

Yizhan Wu , Fei Guo , Ya Liu , Jiajia Li , Wenhui Shi , Laiyang Song , Guangjun Wang , Jiangwei Liu

{"title":"Curcumin mitigates heatstroke-induced myocardial injury by modulating the Akt/Bad/Caspase-3 pathway","authors":"Yizhan Wu , Fei Guo , Ya Liu , Jiajia Li , Wenhui Shi , Laiyang Song , Guangjun Wang , Jiangwei Liu","doi":"10.1016/j.bbrc.2025.151653","DOIUrl":"10.1016/j.bbrc.2025.151653","url":null,"abstract":"<div><div>Heatstroke (HS) presents a major health threat, especially during summer, and is linked to myocardial injury and persistent cardiovascular complications.Curcumin has shown promise in treating myocardial damage, but its mechanisms in HS-induced myocardial damage remain unclear. We integrated curcumin targets from BATMAN-TCM, DGIdb, and PharmMapper, and identified HS-related targets from GeneCards and OMIM. The intersection of these targets was identified using Venn diagrams, and subsequently analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.Protein interactions were analyzed using STRING and visualized in Cytoscape to screen core proteins. Molecular docking was performed with these proteins and curcumin. HS mouse model was constructed for pathological assessments and WB validation of core protein expression. We identified 132 potential therapeutic targets and selected AKT1, Bad, and CASP3 as our targets for validation. Molecular docking indicated that these proteins all have good affinity with curcumin. In HS mouse model, we observed that HS led to significant myocardial cell edema, disordered arrangement, and pronounced mitochondrial swelling accompanied by the destruction of cristae. The application of curcumin effectively alleviated myocardial cell edema and the degree of mitochondrial swelling. WB revealed that HS decreased p-Akt and p-Bad while increasing cleaved-caspase-3. Curcumin treatment reversed these effects, inhibiting HS-induced myocardial cell apoptosis. Our research demonstrates that curcumin effectively safeguards against HS-induced myocardial injury in mice, potentially through the modulation of the Akt/Bad/caspase-3 pathway.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"Article 151653"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic social defeat stress alters DNA methylation profiles of male germ cells in mice.

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-17 DOI: 10.1016/j.bbrc.2025.151650

Hikari Ohno, Yutaka Yamamuro, Shu Aizawa

{"title":"Chronic social defeat stress alters DNA methylation profiles of male germ cells in mice.","authors":"Hikari Ohno, Yutaka Yamamuro, Shu Aizawa","doi":"10.1016/j.bbrc.2025.151650","DOIUrl":"https://doi.org/10.1016/j.bbrc.2025.151650","url":null,"abstract":"<p><p>Chronic exposure to stress disrupts various structural and functional features in living organisms. In addition to the adverse effects of chronic stress on the central nervous system, evidence suggests that chronic stress leads to dysfunction of the male reproductive system. In particular, previous reports have shown that exposure to chronic social defeat stress (cSDS), an animal model of psychosocial stress and depression, induces a reduction in sperm concentration and motility. However, the mechanism by which exposure to cSDS contributes to male reproductive abnormalities remains poorly understood. In the present study, we investigated the effects of cSDS on DNA methylation profiles in germ cells isolated from the testes of C57BL/6J mice. We found that exposing mice to cSDS significantly decreased the 5-methylcytosine levels but not 5-hydroxymethylcytosine levels in germ cells. Furthermore, reduced representation of bisulfite sequencing revealed that cSDS exposure specifically alters the DNA methylation status at gene regulatory regions of transcriptional regulation-related genes. These findings suggest that chronic exposure to psychosocial stress disrupts the male reproductive system through abnormal epigenetic modifications in male germ cell, providing novel insight into the detrimental effects of chronic stress on male germ cell development in the testis.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"151650"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delving into the crucial role of the initial structure in the dynamic and self-assembly of amyloid beta.

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-17 DOI: 10.1016/j.bbrc.2025.151652

Maryam Ghorbaninia, Shirin Doroudgar, Mohamad Reza Ganjalikhany

{"title":"Delving into the crucial role of the initial structure in the dynamic and self-assembly of amyloid beta.","authors":"Maryam Ghorbaninia, Shirin Doroudgar, Mohamad Reza Ganjalikhany","doi":"10.1016/j.bbrc.2025.151652","DOIUrl":"https://doi.org/10.1016/j.bbrc.2025.151652","url":null,"abstract":"<p><p>Alzheimer's disease involves the accumulation of amyloid beta (Aβ) monomers that form oligomers and fibrils in the brain. Studying the Aβ monomer is critical for understanding Aβ assembly and peptide behavior and has implications for drug design. Choosing a starting structure with a higher aggregation tendency for cost-effective MD studies and drug design is crucial. Previous studies have utilized distinct initial conformations, leading to varying results. Hence, this study was conducted to compare different initial conformations using the same MD simulation protocol to investigate the behavior and oligomerization propensity of different starting structures of Aβ during 1μs. The behavior of the monomers and their self-assembly systems were studied thoroughly, and the results revealed that highly helical Aβ monomers which used as starting structures retain high helix content during the simulation, and their tautomerization states did not cause significant changes in the structure. On the other hand, the Aβ extended and S-shaped monomers displayed the fingerprints of the fibril structure, which is believed to be more favorable for self-assembly. Self-assembly behaviors were seen for three S-shaped and three Aβ extended peptides. However, both conformations did not show stable β-sheet intermolecular interaction. For the Aβ16-22 monomer as a fragment of the Aβ that can assemble into fibrils, the impacts of capping and uncapping on the initial structure were also investigated. The results displayed that capped and uncapped structures can form oligomers with β-sheet at termini. However, in the capped state, β-sheet interactions were more stable and remained relatively longer than uncapped.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"151652"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining interactions of animal cells with chloroplasts and their light-induced responses in in vitro cell culture systems.

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-16 DOI: 10.1016/j.bbrc.2025.151622

Kyota Hamashima, Lilingman Fan, Reika Miyagawa, Natsuki Hara, Kazuki Nishida, Hisato Saitoh

{"title":"Examining interactions of animal cells with chloroplasts and their light-induced responses in in vitro cell culture systems.","authors":"Kyota Hamashima, Lilingman Fan, Reika Miyagawa, Natsuki Hara, Kazuki Nishida, Hisato Saitoh","doi":"10.1016/j.bbrc.2025.151622","DOIUrl":"https://doi.org/10.1016/j.bbrc.2025.151622","url":null,"abstract":"<p><p>Chloroplasts are organelles that convert light energy into chemical energy in plants. The potential to integrate chloroplasts into animal cells presents an exciting frontier in synthetic biology, allowing for photo-controllable biochemical processes within these cells. However, the lack of well-established in vitro experimental systems to study chloroplast-animal cell interactions remains a significant challenge. This study investigates the behavior of human cervical cancer HeLa cells and mouse macrophage-like J774.1 cells, along with the light-induced responses of these cells, when introduced into culture media containing spinach-derived chloroplasts. Additionally, we examine isolated cells from Elysia marginata, a sacoglossan sea slug known for its unique ability to acquire and retain functional chloroplasts through a process known as kleptoplasty. Our results show that HeLa cells primarily adhere to chloroplasts with minimal intracellular uptake, while J774.1 cells actively engulf them. Co-incubation with chloroplasts increases the rate of cell death upon light irradiation. In contrast, naturally chloroplast-containing cells from E. marginata exhibit minimal light-induced damage. Excessive reactive oxygen species (ROS) production is observed in HeLa and J774.1 cells co-incubated with chloroplasts under light exposure, suggesting that photoinduced ROS generation contributes to cytotoxicity. These findings highlight three different patterns of interactions between animal cells and chloroplasts and underscore the importance of considering ROS generation induced by light exposure when analyzing chloroplast-animal cell interactions in vitro experimental systems.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"758 ","pages":"151622"},"PeriodicalIF":2.5,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TT8 transcription factor alleviates nickel toxicity in Arabidopsis

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-15 DOI: 10.1016/j.bbrc.2025.151649

Yuhao Hu , Yuxing Peng , Xiaoting Qi

{"title":"The TT8 transcription factor alleviates nickel toxicity in Arabidopsis","authors":"Yuhao Hu , Yuxing Peng , Xiaoting Qi","doi":"10.1016/j.bbrc.2025.151649","DOIUrl":"10.1016/j.bbrc.2025.151649","url":null,"abstract":"<div><div>Nickel (Ni) is a necessary element for plants, but excessive accumulation of Ni in soil causes plant damage, one of which is oxidative stress. Plants utilize secondary metabolite polyphenols such as anthocyanins as antioxidant molecules and metal chelators to cope with heavy metal toxicity. However, the regulatory factors linking polyphenol accumulation to resistance to Ni stress in plants remain unidentified. <em>Arabidopsis</em> TRANSPARENT TESTA8 (TT8) is an essential transcription factor involved in anthocyanin biosynthesis, especially in seeds. Here, we reported that TT8 links polyphenol accumulation to <em>Arabidopsis</em> tolerance to Ni stress. <em>TT8</em> knockout reduced the anthocyanin content in the seed coat, leading to several aspects of Ni toxicity: low seedling survival rates, delayed seed germination, severe membrane lipid peroxidation damage, reduced antioxidant capacity, and low polyphenol (especially epicatechin) abundance. When <em>TT8</em> was overexpressed, these <em>Arabidopsis</em> plants tolerated extreme Ni stress with high survival rates and germination rates and accumulated more polyphenols. On the basis of these data, we concluded that TT8 maintains the antioxidant capacity of <em>Arabidopsis</em> to avoid Ni stress–induced oxidative damage by promoting polyphenol accumulation. Moreover, TT8 not only responds to Ni stress but also positively regulates six flavonoid biosynthesis enzyme-encoding genes (<em>CHS</em>, <em>CHI</em>, <em>F3H</em>, <em>F3ˊH</em>, <em>DFR</em>, and <em>FLS</em>). Therefore, TT8-mediated transcriptional regulation cascades of flavonoid biosynthesis may contribute to its effects on polyphenol accumulation. Collectively, our findings provide deep mechanical insights into how specific transcription factors alleviate excessive Ni toxicity in plants and offer new approaches for the breeding of Ni stress-tolerant crops.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151649"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the antimicrobial activity of carvacrol and its mechanism of action against drug-resistant bacteria

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-15 DOI: 10.1016/j.bbrc.2025.151643

Ziling Zhi , Peng Zhou , Tenghui He , Sisi Chen , Xiping Qian , Yanyan Ye , Wing-Leung Wong , Song Li , Ning Sun , Wenchang Yuan

{"title":"Study of the antimicrobial activity of carvacrol and its mechanism of action against drug-resistant bacteria","authors":"Ziling Zhi , Peng Zhou , Tenghui He , Sisi Chen , Xiping Qian , Yanyan Ye , Wing-Leung Wong , Song Li , Ning Sun , Wenchang Yuan","doi":"10.1016/j.bbrc.2025.151643","DOIUrl":"10.1016/j.bbrc.2025.151643","url":null,"abstract":"<div><div>Drug-resistant bacterial infections have been one of the critical health issues encountered worldwide currently because most conventional antibiotics are losing their effectiveness in clinical treatments. It is thus urgently to identify new antibiotics or alternatives against drug-resistant bacteria. For this purpose, we attempted to seek active compounds from commercially available natural products, which may be one of the fast-tracks to address the drug-resistant bacterial infections. In the present study, we investigated the antibacterial activity, antibacterial mechanism and synergistic effects of carvacrol against a panel of drug-resistant bacteria, including some clinical isolates. The results show that carvacrol (<em>cymophenol</em>), a monoterpenoid phenol, has excellent antibacterial activity. The MIC values against the bacteria examined are found to be 4–16 μg/mL. Our results also suggested that carvacrol might not likely to induce drug-resistance. More importantly, when carvacrol combined with first-line antibiotics, it exhibited good synergistic effects against drug-resistant bacteria. Moreover, in morphological studies, carvacrol could cause <em>B. subtilis</em> 168 elongation and <em>S. aureus</em> BAA-41 enlargement, which may suggest an antibacterial mechanism possibly correlated with the inhibition of bacterial cell division. We further demonstrated that carvacrol facilitated the polymerization of FtsZ that is a critically important protein for regulating bacterial cell division. Furthermore, molecular modeling predicted that carvacrol could interact with T7-loop of FtsZ. The findings of this study suggest that carvacrol may be a potential inhibitor of FtsZ and it could be utilized to combat drug-resistant bacteria in combination with existing antibiotics.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151643"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome of amino acids or IGF1-stimulated pacu muscle cells offers molecular insights and suggests FN1B and EIF3C as candidate markers of fish muscle growth

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-15 DOI: 10.1016/j.bbrc.2025.151648

Erika S. Perez , Rafaela A. Ribeiro , Bruna TT. Zanella , Fernanda LA. Almeida , Josefina Blasco , Daniel Garcia de la serrana , Maeli Dal-Pai-Silva , Bruno OS. Duran

{"title":"Proteome of amino acids or IGF1-stimulated pacu muscle cells offers molecular insights and suggests FN1B and EIF3C as candidate markers of fish muscle growth","authors":"Erika S. Perez , Rafaela A. Ribeiro , Bruna TT. Zanella , Fernanda LA. Almeida , Josefina Blasco , Daniel Garcia de la serrana , Maeli Dal-Pai-Silva , Bruno OS. Duran","doi":"10.1016/j.bbrc.2025.151648","DOIUrl":"10.1016/j.bbrc.2025.151648","url":null,"abstract":"<div><div>Study of fish skeletal muscle is essential to understand physiological or metabolic processes, and to develop programs searching for increased muscle mass and meat production. Amino acids (AA) and IGF1 stimulate processes that lead to muscle growth, but their signaling pathways and molecular regulation need further clarification in fish. We obtained the proteome of pacu (<em>Piaractus mesopotamicus</em>) cultured muscle cells treated with AA or IGF1, which induced the differential abundance of 67 and 53 proteins, respectively. Enrichment analyses showed that AA modulated histone methylation, cell differentiation, and metabolism, while IGF1 modulated ATP production and protein synthesis. In addition, we identified molecular networks with candidate markers that commonly regulate fish muscle cells: FN1B and EIF3C, respectively up- and down-regulated by both treatments. FN1B was related to cell proliferation, protein synthesis, and muscle repair, while EIF3C connected with negative regulators of muscle growth. Their gene expression was evaluated in pacu and Nile tilapia (<em>Oreochromis niloticus</em>) after nutrient manipulation, with <em>fn1b</em> increased during refeeding and <em>eif3c</em> increased during fasting in both species. Our work helps clarify the molecular regulation by AA or IGF1 and suggests that FN1B and EIF3C could be potential stimulatory and inhibitory biomarkers of fish muscle growth.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151648"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman spectroscopic modality to examine therapeutic efficacy of Galectin-3 inhibitor in prostate cancer

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-15 DOI: 10.1016/j.bbrc.2025.151646

Samaneh Ghazanfarpour , Alireza Sheikhsofla , Monireh Pourrahimi , Satish Sharma , Andrew Skomra , Anna Sharikova , Stanley A. Schwartz , Supriya D. Mahajan , Alexander Khmaladze , Ravikumar Aalinkeel

{"title":"Raman spectroscopic modality to examine therapeutic efficacy of Galectin-3 inhibitor in prostate cancer","authors":"Samaneh Ghazanfarpour , Alireza Sheikhsofla , Monireh Pourrahimi , Satish Sharma , Andrew Skomra , Anna Sharikova , Stanley A. Schwartz , Supriya D. Mahajan , Alexander Khmaladze , Ravikumar Aalinkeel","doi":"10.1016/j.bbrc.2025.151646","DOIUrl":"10.1016/j.bbrc.2025.151646","url":null,"abstract":"<div><div>Glycoproteins, such as Galectin-3 (Gal-3) and Prostate Specific Membrane Antigen (PSMA), are functional proteins involved in numerous biological activities that include cell apoptosis, angiogenesis, and inflammation. Downregulation of both in the highly metastatic human Prostate Cancer (CaP) cell line PC-3 reduces tumor growth. We used Raman Spectroscopy (RS) to examine the effect of a potent Gal-3 inhibitor (GB1107) in CaP cell lines of varying metastatic potential, namely PC-3, DU-145 and LNCaP. All three cancer lines had distinct Raman signatures. Raman spectra from PC-3, DU-145 and LNCaP cells treated with GB1107, compared to the untreated cells as controls, showed significant differences corresponding to changes in phosphatidylinositol (peak at 596 cm<sup>−1</sup>), O–P–O stretching DNA (786 cm<sup>−1</sup>), lipid/phospholipid DNA backbone (1090-1100 cm<sup>−1</sup>), nucleic acid, lipid, protein (amide III) (1296-1305 cm<sup>−1</sup>), fatty acid (1440 cm<sup>−1</sup>), and protein (amid I) (1655 cm<sup>−1</sup>), suggesting that DNA phosphate backbone may become unstable with cancer progression, facilitating cancer cell metastasis. Our data suggests that Gal-3 inhibitor induces significant alterations in major biochemical constituents, such as lipids, proteins, and nucleic acids, which may lead to structural and molecular changes in the cancerous prostate tissue. To further analyze these spectral differences, Singular Value Decomposition (SVD) and Linear Discriminant Analysis (LDA) were applied for classification, enabling effective differentiation between treated and untreated CaP cell lines. This highlights the therapeutic potential of Gal-3 inhibitor in prevention of CaP progression and metastases.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"757 ","pages":"Article 151646"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0