Biochemical and biophysical research communications最新文献_第2页

Estrogen receptors regulate sex disparity in the immune responses during zebrafish liver regeneration following partial hepatectomy. 雌激素受体调节斑马鱼肝部分切除术后肝再生过程中免疫反应的性别差异。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-11-01 DOI: 10.1016/j.bbrc.2024.150937

Mingkai Zhu, Yan Li, Dong Liu, Zhiyuan Gong

引用次数: 0

Expanding the occurrence of antimalarial metabolites in dorid nudibranch Hypselodoris tryoni 扩大多鳃裸鳃亚纲 Hypselodoris tryoni 中抗疟代谢物的出现范围。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-30 DOI: 10.1016/j.bbrc.2024.150921

I Wayan Mudianta , Josephine Elizabeth Siregar , Andita Fitri Mutiara Rizki , Wihda Aisarul Azmi , Normalita Eka Pravitasari , Gusnia Meilin Gholam , Fadillaisyia Riandani Putri , Rhesi Kristiana , Ni Kadek Dita Cahyani , I Made Artika

{"title":"Expanding the occurrence of antimalarial metabolites in dorid nudibranch Hypselodoris tryoni","authors":"I Wayan Mudianta , Josephine Elizabeth Siregar , Andita Fitri Mutiara Rizki , Wihda Aisarul Azmi , Normalita Eka Pravitasari , Gusnia Meilin Gholam , Fadillaisyia Riandani Putri , Rhesi Kristiana , Ni Kadek Dita Cahyani , I Made Artika","doi":"10.1016/j.bbrc.2024.150921","DOIUrl":"10.1016/j.bbrc.2024.150921","url":null,"abstract":"<div><div>This study examined the antimalarial activity of a furanosesquiterpene, furodysinin, one of the major metabolites of the dorid nudibranch <em>Hypselodoris tryoni</em>. The nudibranchs were collected from Balinese waters and the metabolites were purified by chromatography. <em>Ex vivo</em> rodent malaria <em>Plasmodium berghei</em> assays were conducted to determine the metabolite antimalarial activity. <em>In silico</em> molecular docking was employed to investigate the interaction between furodysinin against wild-type <em>P. berghei</em> and atovaquone-resistant <em>P. berghei</em> (Y268C). This study reported for the first time that the furodysinin displayed a promising antimalarial activity based on the <em>ex vivo</em> tests against wild-type <em>P. berghei</em> and atovaquone-resistant <em>P. berghei</em>. <em>In silico</em> molecular docking study showed that furodysinin inhibits the parasite mitochondrial cytochrome <em>b</em> (cyt <em>b</em>) by binding to the protein Qo pocket (ef-helix) where it interacts with residue 268, the mutation of which is known to confer resistance to atovaquone. Furodysinin binds to the mutated tyrosine at residue 268, which has changed to cysteine, forming an alkyl bond with C268 at a distance of 4.6 Å. Therefore, we predict that furodysinin has a target in <em>Plasmodium</em> mitochondria.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The structural Basis of NMN synthesis catalyzed by NadV from Haemophilus ducreyi 杜克雷嗜血杆菌 NadV 催化 NMN 合成的结构基础

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150889

Zheng-Juan Wang , Jia Yuan , Lin Tang

{"title":"The structural Basis of NMN synthesis catalyzed by NadV from Haemophilus ducreyi","authors":"Zheng-Juan Wang , Jia Yuan , Lin Tang","doi":"10.1016/j.bbrc.2024.150889","DOIUrl":"10.1016/j.bbrc.2024.150889","url":null,"abstract":"<div><div>NMN is a precursor in the biosynthesis of NAD<sup>+</sup>, a molecule that plays a crucial role within cells. Supplementation with NMN can elevate NAD<sup>+</sup> levels in the blood, improving symptoms of diabetes, neurodegenerative diseases, and cancer, as well as providing anti-aging benefits. <em>Escherichia coli</em> was engineered to heterologously express nicotinamide phosphoribosyltransferase (Nampt), enabling the recombinant <em>E. coli</em> to synthesize NAD derivatives from nicotinamide. The 3D structure of Nadv complexed with NAM and NMN was determined to explore the molecular mechanism by which Nadv catalyzes NMN synthesis. NAM binds at two sites: one at the catalytic site and one at the allosteric binding site, while NMN binds exclusively at the catalytic site. In both structural models, a loop between β15 and β16 is missing, likely due to its high flexibility, leading to diffuse electron density. Compared with other resolved Nampt structures, an additional 12-amino-acid loop was identified after α-helix 12 near the catalytic site. This study lays the groundwork for the engineering of Nadv, facilitating its efficient application in biological synthesis of NMN.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical characterization of microRNA mixtures based on Molecular Beacons 基于分子信标的 microRNA 混合物的生物物理表征。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150913

Micaela Riscado , Leonor Mariano , Carla Cruz , Chantal Pichon , Fani Sousa

{"title":"Biophysical characterization of microRNA mixtures based on Molecular Beacons","authors":"Micaela Riscado , Leonor Mariano , Carla Cruz , Chantal Pichon , Fani Sousa","doi":"10.1016/j.bbrc.2024.150913","DOIUrl":"10.1016/j.bbrc.2024.150913","url":null,"abstract":"<div><div>Diverse studies have shown a relationship between dysregulated microRNAs (miRNAs), including miRNA-29b and miRNA-9, and several diseases. So, it is hypothesized that miRNAs can be studied as potential agents to be exploited in biomedical applications, due to their ability to take part in gene expression regulation at a post-transcriptional level. Considering the possibility of using miRNAs, it is important to characterize and validate this bioproduct, structurally and functionally. The goal of this work is to optimize an assay that can detect and biophysically characterize a miRNA sample without interference from the respective precursor form, by using molecular beacons (MB). MBs are hairpin-shaped probes composed of nucleic acid labeled with a quencher at the 3′ end and a fluorophore (reporter) at the 5’ end. Here, MB loops were designed so MB-9-1 and MB-29-1 would be complementary to the miRNA-9-1-5p and the miRNA-29b-1-3p, respectively. The MBs designed in this work specifically identified each target miRNA, even in artificial mixtures or complex samples, and the obtained fluorescence was directly proportional to miRNA concentration. Even if the precursor forms (pre-miRNAs) were present in the samples, no significant signal was shown, allowing the distinction between both forms. The outcomes of this work confirm the MBs potential to assess and characterize miRNA samples to be exploited in biochemical, biophysical, or biomedical fields.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced expressions of TCA cycle genes during aging in humans and mice 人类和小鼠衰老过程中 TCA 循环基因的表达减少

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150917

Chao Zhang , Zhiyao Fu , Ren Zhang

{"title":"Reduced expressions of TCA cycle genes during aging in humans and mice","authors":"Chao Zhang , Zhiyao Fu , Ren Zhang","doi":"10.1016/j.bbrc.2024.150917","DOIUrl":"10.1016/j.bbrc.2024.150917","url":null,"abstract":"<div><div>Aging is associated with a decline in physiological functions and an increased risk of metabolic disorders. The liver, a key organ in metabolism, undergoes significant changes during aging that can contribute to systemic metabolic dysfunction. This study investigates the expression of genes involved in the tricarboxylic acid (TCA) cycle, a critical pathway for energy production, in the aging liver. We analyzed RNA sequencing data from the Genotype-Tissue Expression (GTEx) project to assess age-related changes in gene expression in the human liver. To validate our findings, we conducted complementary studies in young and old mice, examining the expression of key TCA cycle genes using quantitative real-time PCR. Our analysis of the GTEx dataset revealed a significant reduction in the expression of many genes that are critical for metabolism, including fat mass and obesity associated (FTO) and adiponectin receptor 1 (ADIPOR1). The most overrepresented pathway among the statistically enriched ones was the TCA cycle, with multiple genes exhibiting downregulation in older humans. This reduction was consistent with findings in aging mice, which also showed decreased expression of several TCA cycle genes. These results suggest a conserved pattern of age-related downregulation of TCA cycle, potentially leading to diminished mitochondrial function and energy production in the liver. The reduced expression of TCA cycle genes in the aging liver may contribute to metabolic dysfunction and increased susceptibility to age-related diseases. Understanding the molecular basis of these changes provides new insights into the aging process and highlights potential targets for interventions aimed at promoting healthy aging and preventing metabolic disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory mechanisms of macrophage–myofibroblast transdifferentiation: A potential therapeutic strategy for fibrosis 巨噬细胞-肌成纤维细胞转分化的调控机制：纤维化的潜在治疗策略

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150915

Junchao Zhang, Jinfa Huang, Qian Yang, Lingling Zeng, Kaixian Deng

引用次数: 0

Ethyl gallate ameliorates diabetes-induced Alzheimer's disease-like phenotype in rats via activation of α7 nicotinic receptors and mitigation of oxidative stress 没食子酸乙酯通过激活α7烟碱受体和减轻氧化应激改善糖尿病诱导的大鼠阿尔茨海默病样表型

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150925

Kushagra Nagori , Madhulika Pradhan , Kartik T. Nakhate

{"title":"Ethyl gallate ameliorates diabetes-induced Alzheimer's disease-like phenotype in rats via activation of α7 nicotinic receptors and mitigation of oxidative stress","authors":"Kushagra Nagori , Madhulika Pradhan , Kartik T. Nakhate","doi":"10.1016/j.bbrc.2024.150925","DOIUrl":"10.1016/j.bbrc.2024.150925","url":null,"abstract":"<div><div>Cognitive decline, an important comorbidity of type 2 diabetes (T2D), is attributed to oxidative stress and impaired cholinergic signaling in the brain. The α7 nicotinic acetylcholine receptor (α7nAChR) is densely distributed in the hippocampus and cortex, and exerts neuroprotective and procognitive actions. Ethyl gallate (EG), a natural phenolic antioxidant compound, showed high <em>in-silico</em> binding affinity towards α7nAChR and brain penetrability. Therefore, the present study aimed to evaluate the involvement of α7nAChR in the potential of EG to ameliorate T2D-induced Alzheimer's disease-like condition. T2D was induced by intraperitoneal (i.p.) injection of streptozotocin (35 mg/kg) in rats on high-fat diet. Diabetic animals were treated with EG (10 and 20 mg/kg, i.p.) for four weeks, and their learning and memory performance was evaluated by the Morris water maze (MWM). Further, the brains were subjected to biochemical analysis of antioxidants like glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), and oxidative stress marker malonaldehyde (MDA). While diabetic rats showed a significant decline in cognitive performance in the MWM, a substantial improvement was noticed following EG treatment. Further, the diabetes-associated reductions in GSH, SOD, and CAT levels, along with increased MDA contents in the brain, were effectively restored by EG. Interestingly, pre-treatment with α7nAChR antagonist methyllycaconitine (1 mg/kg, i.p.) attenuated the effects of EG on behavioral and biochemical parameters. The results suggest that EG may augment cholinergic signaling in the brain via α7nAChR to mitigate oxidative stress, consequently alleviating T2D-associated dementia. Therefore, EG could be a potential candidate for addressing cognitive impairment comorbid with T2D.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting threonine deaminase with chiral Au NPs: A novel strategy for E. coli inhibition 用手性金纳米粒子靶向苏氨酸脱氨酶：抑制大肠杆菌的新策略

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150924

He Wang , Haidu Yuan , Jianhao Zhang , Wenjing Yan

{"title":"Targeting threonine deaminase with chiral Au NPs: A novel strategy for E. coli inhibition","authors":"He Wang , Haidu Yuan , Jianhao Zhang , Wenjing Yan","doi":"10.1016/j.bbrc.2024.150924","DOIUrl":"10.1016/j.bbrc.2024.150924","url":null,"abstract":"<div><div>Bacterial infections are becoming a significant threat to global human health due to the growing prevalence of biofilm-related infections and the rise in antibiotic resistance. D/<span>l</span>-cysteine functionalized chiral gold nanoparticles (D/P-Au NPs or L/P-Au NPs) have demonstrated a potent antibacterial effect against <em>E. coli</em>, while the mechanism remains to be elucidated through additional research. Threonine deaminase (TD) is a crucial enzyme involved in branched-chain amino acid (BCAA) biosynthesis in <em>E. coli</em> and is involved in cysteine's antimicrobial effects. This study investigated the interaction between chiral Au NPs (D/P-Au NPs or L/P-Au NPs) and TD as well as its effect on enzyme activity. It demonstrates that chiral Au NPs interact with TD through hydrophobic forces, forming a ground state complex that induces changes in the secondary structure of TD and reduces enzyme activity in a concentration-dependent manner. We found that the exogenous supplementation of isoleucine and valine (2 mg/mL) significantly reduced the antibacterial activity of chiral Au NPs, especially for L/P-Au NPs. The proteomics results indicate that the expression of <em>ilvA</em> and <em>ilvB</em> was down-regulated after L/P-Au NPs treatment, which would interfere with the synthesis of BCAAs. These results demonstrate that chiral Au NPs cause cell death of <em>E. coli</em> partly due to inhibition of TD enzyme activity and the synthesis of branched-chain amino acids.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of MCL1 attenuates irritable bowel syndrome by regulating cuproptosis: Screening and validation 过表达 MCL1 可通过调节杯突减少肠易激综合征：筛选与验证

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-29 DOI: 10.1016/j.bbrc.2024.150926

Taohong He , Jian Kang , Xiao Tang , Yuqi Wu , Liangliang Hao

{"title":"Overexpression of MCL1 attenuates irritable bowel syndrome by regulating cuproptosis: Screening and validation","authors":"Taohong He , Jian Kang , Xiao Tang , Yuqi Wu , Liangliang Hao","doi":"10.1016/j.bbrc.2024.150926","DOIUrl":"10.1016/j.bbrc.2024.150926","url":null,"abstract":"<div><div>Irritable bowel syndrome (IBS) is a type of chronic bowel disorder with a poorly understood pathophysiology. Recently, the imbalance of copper has been reported to influence the progression of IBS, suggesting cuproptosis, a new type of copper-induced cell death, may play a role in IBS. This study found 17 cuproptosis-related differentially expressed genes in IBS through bioinformatic analysis. Six hub genes were identified after the protein-protein interaction network analysis, namely myeloid cell leukemia 1 (<em>MCL1</em>), epidermal growth factor receptor 2, cadherin-associated protein beta 1, solute carrier family 25 members 37, solute carrier family 39 members 14, and six transmembrane epithelial antigens of the prostate 3. We selected <em>MCL1</em> for further verification. Human normal colon epithelial cell line (NCM460) was used to construct models of IBS or cuproptosis <em>in vitro</em> by lipopolysaccharide (LPS) or LPS combined with copper (II) chloride (CuCl<sub>2</sub>)<em>.</em> We observed that overexpression of <em>MCL1</em> promoted cell viability and proliferation ability, and inhibited the secretion of inflammatory factors and expression of Bax and caspase-3 of NCM460 cells treated with LPS or LPS combined with CuCl<sub>2</sub>. In addition, up-regulated <em>MCL1</em> significantly suppressed the protein levels of ferredoxin 1 and lipoyl synthase, two key regulators of cuproptosis. In conclusion, our study demonstrates that cuproptosis is involved in IBS and identifies a cuproptosis-related gene, <em>MCL1</em>, that helps alleviate IBS by promoting cell growth, reducing inflammation, and suppressing cuproptosis, making it a promising therapeutic target in IBS.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mating modifies oxidative stress in the brain and confers protection against Parkinson's Disease in a Drosophila model 在果蝇模型中，交配可改变大脑中的氧化应激并使其免受帕金森病的侵袭

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-28 DOI: 10.1016/j.bbrc.2024.150911

Zhi-Hua Liu , Yuyin Zhai , Yanzhou Xia , Qiaoming Liao

{"title":"Mating modifies oxidative stress in the brain and confers protection against Parkinson's Disease in a Drosophila model","authors":"Zhi-Hua Liu , Yuyin Zhai , Yanzhou Xia , Qiaoming Liao","doi":"10.1016/j.bbrc.2024.150911","DOIUrl":"10.1016/j.bbrc.2024.150911","url":null,"abstract":"<div><div>Mating exerts profound and multifaceted effects on the physiology of female insects, particularly influencing metabolic alterations and bolstering stress resilience. <em>Drosophila melanogaster</em> has emerged as an excellent model to investigate the mechanism of neurodegenerative diseases. However, interplay between mating and its impact on the <em>Drosophila</em> brain remains a tantalizing enigma, awaiting elucidation. Herein, we reported that mating significantly improved the climbing and jumping activity in mated females compared to the virgins in <em>Drosophila</em>. Mating also reduced oxidative stress in the brain. Based on the results, we found that, mated females exhibited better behavioral performance and fewer loss of dopaminergic (DA) neurons than unmated females in <em>PINK1</em> RNAi flies, a well-established Parkinson's disease (PD) model. Further study demonstrated that mating led to decreased iron content in the brain, a process associated with decreased Transferrin 1 (Tsf1) and Malvolio (Mvl) and increased ferritin. Additionally, mating inhibited expression of Duox and Nox, two NADPH oxidases in <em>Drosophila</em>. Furthermore, Kr-h1, a transcription factor of JH, acted downstream of mating to regulate genes involved in iron metabolism and NADPH oxidases. Collectively, the findings suggested a pivotal role of mating in regulating iron metabolism and NADPH oxidases in the brain of <em>Drosophila</em>. Consequently, considering mating status is imperative in scientific research, particularly in the context of neurological disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0