Biochemical and biophysical research communications最新文献

{"title":"Comparison of the effects between catalase and superoxide dismutase on regulating macrophage inflammatory response and protecting osteogenic function of periodontal ligament cells","authors":"Bijun Zhu,&nbsp;Lutong Song,&nbsp;Mengchen Li,&nbsp;Mingyue Cheng,&nbsp;Wenyue Tang,&nbsp;Leiying Miao","doi":"10.1016/j.bbrc.2025.151523","DOIUrl":"10.1016/j.bbrc.2025.151523","url":null,"abstract":"<div><h3>Background</h3><div>Reactive oxygen species (ROS) have been confirmed closely associated with the pathological process of periodontitis, but the specific roles played by different ROS types are still to be investigated. Catalase (CAT) and Superoxide dismutase (SOD) specifically eliminate hydrogen peroxide (H₂O₂) and superoxide anion (O₂^•-), respectively. We for the first time compare the effects and mechanisms of CAT and SOD in protecting periodontal ligament cells (PDLCs) against oxidative damage, reducing the expression of macrophage inflammatory factors, and preserving the osteogenic differentiation function of PDLCs by modulating the inflammatory environment.</div></div><div><h3>Methods</h3><div>CAT or SOD in combination with lipopolysaccharide (LPS) were added to the culture medium of RAW 264.7 and PDLCs. The intracellular ROS level, lipid peroxidation and DNA damage were observed by confocal microscope. Inflammation levels were assessed by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. A co-culture system of macrophages and PDLCs was established, and the osteogenic differentiation of PDLCs was evaluated by alkaline phosphatase staining, alizarin red S staining, RT-qPCR and Western blot. Finally, differentially expressed genes (DEGs) in CAT and SOD were detected by RNA sequencing and the biological functions and signaling pathways involved were analyzed.</div></div><div><h3>Results</h3><div>CAT or SOD can effectively inhibit intracellular ROS levels, lipid peroxidation and DNA damage, as well as increase the levels of antioxidative molecules and decrease the levels of inflammatory factors. SOD increased the levels of antioxidative molecules more strongly, while CAT reduced inflammatory factors more effectively. The RNA sequencing results indicate that CAT exhibits stronger inhibitory effects on inflammation-related signaling pathways, which could account for the observed differences.</div></div><div><h3>Conclusions</h3><div>In this study, we observed differential antioxidant and anti-inflammatory effects between CAT and SOD, which may be associated with CAT's better inhibition of the activation of inflammatory pathways. Our study will provide scientific references for the future development of highly selective ROS- scavenging antioxidant drugs.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"756 ","pages":"Article 151523"},"PeriodicalIF":2.5,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-27 deletion has neuroprotective effects in the acute ischemic stage of cerebral infarction","authors":"Takashi Furukawa ,&nbsp;Yasunobu Miyake ,&nbsp;Hiroshi Ito ,&nbsp;Atsushi Ogata ,&nbsp;Hajime Maeyama ,&nbsp;Yukiko Nakahara ,&nbsp;Fumitaka Yoshioka ,&nbsp;Jun Masuoka ,&nbsp;Hiroki Yoshida ,&nbsp;Tatsuya Abe","doi":"10.1016/j.bbrc.2025.151581","DOIUrl":"10.1016/j.bbrc.2025.151581","url":null,"abstract":"<div><div>Post-ischemic inflammation is an essential step in the progression of brain ischemia-reperfusion injury. Cytokines such as interleukins 17 and 23 (IL-17, IL-23) have been involved in stroke. IL-27 is a member of the IL-12 family that consists of IL-27p28 and Epstein-Barr virus-induced gene 3 (EBI3), having anti-inflammatory properties and regulating T cell polarization and cytokine production. However, whether IL-27 plays an important role in the acute stage of brain ischemia remains unclear. In the acute stage, IL-27 was upregulated after intracerebral ischemia in wild-type mice while mice lacking IL-27 showed decreased infarction area and suppressed inflammatory cytokines. These findings suggest that IL-27 may be involved in cerebral ischemia and could be a potential therapeutic target for mitigating inflammation and avoiding increasing the initial damage in cerebral ischemia.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"755 ","pages":"Article 151581"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel subcellular regulatory mechanisms of protein homeostasis and its implications in amyotrophic lateral sclerosis","authors":"Aisheng Zhan ,&nbsp;Keke Zhong ,&nbsp;Kejing Zhang","doi":"10.1016/j.bbrc.2025.151582","DOIUrl":"10.1016/j.bbrc.2025.151582","url":null,"abstract":"<div><div>Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron degenerative disorder. Protein aggregates induce various forms of neuronal dysfunction and represent pathological hallmarks in ALS patients. Reducing protein aggregates could be a promising therapeutic strategy for ALS. While most studies have focused on cytoplasmic protein homeostasis, neurons adaptively reduce aggregates across subcellular compartments during stress through previously uncharacterized mechanisms. Here, we summarize novel compartment-specific proteostatic mechanisms: (1) the ERAD/RESET pathways, (2) HSPs-mediated nuclear sequestration, (3) mitochondrial aggregate import (MAGIC), (4) neurite-localized UPS/autophagosome and NMP, and (5) exopher-mediated extracellular disposal. These mechanisms collectively ensure cellular stress adaptation and provide novel therapeutic targets for ALS treatment.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"756 ","pages":"Article 151582"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor TaWHY2-6A acts as a positive regulator in response to drought tolerance in transgenic plants","authors":"Yang Yu ,&nbsp;Conglei Wang ,&nbsp;Xiao Zhang ,&nbsp;Jianhe Wang ,&nbsp;Mengting Li ,&nbsp;Tianqi Song ,&nbsp;Dan Liang ,&nbsp;Gang Feng","doi":"10.1016/j.bbrc.2025.151580","DOIUrl":"10.1016/j.bbrc.2025.151580","url":null,"abstract":"<div><div>Drought stress severely affects wheat yield, and Whirly (WHY) transcription factors (TFs) are essential in modulating plant tolerance to abiotic stresses. In this study, we identified six WHY members in the wheat whole-genome database, categorized into Group I and Group II, with three homologous <em>WHY</em> genes in each group. From the four selected drought-responsive candidate genes with upregulated expression, we focused on <em>TaWHY2-6A</em>, which was significantly upregulated under drought stress. Under drought conditions, <em>TaWHY2-6A</em> transgenic <em>Arabidopsis</em> exhibited significantly higher chlorophyll content and better growth status compared to wild-type (WT) plants, indicating that TaWHY2-6A enhances drought resistance in transgenic <em>Arabidopsis</em>. In contrast, wheat lines with silenced-<em>TaWHY2-6A</em> exhibited a more severe wilting phenotype following drought treatment, accompanied by elevated levels of H₂O₂ and O₂^.-, and reduced antioxidant enzyme activity. These findings suggest that the wheat <em>TaWHY2-6A</em> gene positively regulates drought resistance under drought stress. This research provides a theoretical basis and valuable genetic resources for drought-resistance breeding in wheat.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"755 ","pages":"Article 151580"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “ATG8 in single membranes: Fresh players of endocytosis and acidic organelle quality control in cancer, neurodegeneration, and inflammation” [Biochem. Biophys. Res. Commun. 749 (2025) 151384]","authors":"Ben B. Wang ,&nbsp;Pirjo M. Apaja","doi":"10.1016/j.bbrc.2025.151549","DOIUrl":"10.1016/j.bbrc.2025.151549","url":null,"abstract":"","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"755 ","pages":"Article 151549"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SP1 regulates porcine primary adipocyte differentiation by modulating BAMBI transcriptional activity","authors":"Xiaoyan Ren ,&nbsp;Xiaoyu Wang ,&nbsp;Yi Zeng ,&nbsp;Xiaochang Chen ,&nbsp;Yuting Cui ,&nbsp;Lingzhi Liu ,&nbsp;Xinru Chen ,&nbsp;Si Liu ,&nbsp;Hongxu Zhong ,&nbsp;Ping Liao ,&nbsp;Yan Shen ,&nbsp;Kuilong Huang","doi":"10.1016/j.bbrc.2025.151576","DOIUrl":"10.1016/j.bbrc.2025.151576","url":null,"abstract":"<div><div>Obesity is a critical metabolic disorder in modern society, necessitating urgent research and intervention. Compared to mice, pigs are increasingly been recognized as valuable model organisms for such studied due to their closer genetic similarity to humans. The BAMBI gene plays a pivotal role in adipocyte differentiation and adipose tissue development, rendering it a potent target for exploring the mechanisms of obesity and related metabolic disorders. This study conducted an in-depth exploration of the intricate regulatory mechanisms governing the BAMBI gene, with a particular emphasis on the transcription factor SP1. Our experiments strongly provided that SP1 modulates BAMBI transcription by directly binding to its promoter. Using primary porcine adipocytes, we further demonstrated that SP1 regulates adipocyte differentiation occurs via the BAMBI gene. These findings enhance understanding of the critical role played by the BAMBI gene in adipocyte differentiation and establish a theoretical foundation for novel therapeutic strategies targeting obesity and type 2 diabetes. Importantly, this study emphasizes the potential of <em>S</em>P-BAMBI pathways in interventions within the context of metabolic diseases, addressing the global challenges posed by obesity and its associated complications.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"756 ","pages":"Article 151576"},"PeriodicalIF":2.5,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of optimal culture conditions for Gedaniella panicellus GPYS21 (Fragilariaceae) as a high-yield bioresource for palmitoleic acid and fucoxanthin production","authors":"Kichul Cho ,&nbsp;Eun Song Kim ,&nbsp;Hyunji Ki ,&nbsp;Keun-Yong Kim ,&nbsp;Cheol-Ho Pan ,&nbsp;Hyun-Ju Hwang ,&nbsp;Sung Min An","doi":"10.1016/j.bbrc.2025.151579","DOIUrl":"10.1016/j.bbrc.2025.151579","url":null,"abstract":"<div><div><em>Gedaniella panicellus</em>, a marine diatom belonging to the family Fragilariaceae, has not yet been explored for its biotechnological potential. This study aimed to optimize the growth of <em>G. panicellus</em> using response surface methodology and assess its cellular biochemical composition to verify the production of value-added compounds. The strain was identified through morphological and phylogenetic analyses, with optimal conditions of 20.50 °C, pH 7.33, and 42.32 PSU salinity. Its biochemical profile revealed 24.38 % proteins, 33.05 % carbohydrates, and 37.28 % lipids. Fatty acid analysis showed that the <em>G. panicellus</em> produces a high level of palmitoleic acid (62.37 %), exceeding the yields from macadamia nut and other microalgae. Pigment analysis indicated significant fucoxanthin production (9.21 mg/g), along with diadinoxanthin (2.21 mg/g), and <em>β</em>-carotene (0.49 mg/g) contents. Additionally, the strain synthesises various essential and nonessential amino acids. These findings highlight <em>G. panicellus</em>—first recorded in South Korea—as a promising bioresource for palmitoleic acid and carotenoid production, with potential applications in cosmetics and nutraceuticals.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"756 ","pages":"Article 151579"},"PeriodicalIF":2.5,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant defense response mediated by DAF-16 attenuates toxicity of herbicides glyphosate and glufosinate ammonium on Caenorhabditis elegans","authors":"Lili Dai ,&nbsp;Hongkun Bao ,&nbsp;Lei Yu","doi":"10.1016/j.bbrc.2025.151577","DOIUrl":"10.1016/j.bbrc.2025.151577","url":null,"abstract":"<div><div>Glyphosate and glufosinate ammonium are widely used herbicides in agriculture for weed elimination. These unused glyphosate and glufosinate ammonium are discharged into environmental water, soil, and even in the food chain cycle, which greatly threatens ecosystems and human health. However, the toxicological mechanism of glyphosate and glufosinate ammonium on soil non-target organisms remains largely unknown. To investigate the toxic effects of glyphosate and glufosinate ammonium on soil non-target organisms, <em>Caenorhabditis elegans</em> which is directly exposed to various pesticides in the soil was selected as the object of study. In this study, our results showed that the locomotion, growth, reproduction, and lethality of <em>C. elegans</em> exposed to different concentrations of a mixture of glyphosate and glufosinate ammonium are suppressed. Exposure to a mixture of glyphosate and glufosinate ammonium increases the production of reactive oxygen species, accelerating senescence and shortening the lifespan of <em>C. elegans</em>. The exposure to a mixture of glyphosate and glufosinate ammonium activates antioxidant defense responses mediated by DAF-16 and autophagy mediated by SKN-1. Our research provides a novel perspective on the mechanism underlying antioxidant defense response-mediated detoxification of pesticide residues in soil non-target organisms.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"756 ","pages":"Article 151577"},"PeriodicalIF":2.5,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MPO and its role in cancer, cardiovascular and neurological disorders: An update","authors":"Kosar Jannesar,&nbsp;Hamid Soraya","doi":"10.1016/j.bbrc.2025.151578","DOIUrl":"10.1016/j.bbrc.2025.151578","url":null,"abstract":"<div><div>Myeloperoxidase (MPO) is an enzyme that contains a heme group, found mostly in neutrophils and in small amounts in monocytes and plays a major role in their anti-microbial activity. However, excessive levels of MPO have been linked to various disorders and identified as a major cause of tissue destruction. Inhibiting its activity can reduce the severity and extent of tissue damage. Over activity of MPO during chronic inflammation has been shown to be involved in tumorigenesis by inducing a hyper-mutagenic environment through oxidant interaction with DNA, causing DNA modification. Vascular endothelium is one of the most important targets of MPO and high levels have been associated with increased rates of cardiomyopathy, ischemic stroke, heart failure, myocardial infarction, and atrial fibrillation. Therefore, it may be considered a therapeutic target in the treatment of cardiovascular disorders. MPO also participates in the pathogenesis of neurodegenerative diseases. For example, an increase in MPO levels has been observed in the brain tissue of patients with Alzheimer's, Multiple sclerosis (MS), and Parkinson's diseases. In Alzheimer's disease, active MPO is mostly found in the location of beta amyloids and microglia. Therefore, targeting MPO may be a potential treatment and prevention strategy for neurological disorders. This review will discuss MPO's physiological and pathological role in cancer, cardiovascular, and neurological disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"755 ","pages":"Article 151578"},"PeriodicalIF":2.5,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposon mutagenesis identifies acid resistance and biofilm genes as Shigella sonnei virulence factors in Caenorhabditis elegans infection","authors":"Bao Chi Wong ,&nbsp;Fong Yoke Ling ,&nbsp;Qasim Ayub ,&nbsp;Hock Siew Tan","doi":"10.1016/j.bbrc.2025.151546","DOIUrl":"10.1016/j.bbrc.2025.151546","url":null,"abstract":"<div><div>Identifying essential genes in bacterial pathogens during infection can enhance knowledge and provide novel targets for antimicrobial agents’ development. Currently, only <em>Shigella flexneri</em> essential genes during <em>in vitro</em> growth have been experimentally identified<em>.</em> This study used transposon insertion sequencing (TIS) to identify <em>Shigella sonnei</em> essential genes during <em>Caenorhabditis elegans</em> infection. 498 genes were predicted to be essential in <em>S. sonnei</em> during growth on nutrient-rich media. Some genes previously predicted to be essential in <em>Shigella</em> were found non-essential in <em>S. sonnei,</em> such as acetyl metabolism genes (<em>aceEF, lpdA</em>) and sulphate transport genes (<em>cysA, cyst, cysW</em>). Finally, 217 genes were predicted as <em>S. sonnei</em> virulence genes during infection, including acid resistance and biofilm formation genes which was not linked to <em>S. sonnei</em> virulence previously.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"754 ","pages":"Article 151546"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}