Biochemical and biophysical research communications最新文献_第10页

Exosomal 4EBP1 promotes head and neck cancer progression via regulating mitochondrial fission 外泌体4EBP1通过调节线粒体分裂促进头颈癌进展

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151735

Iyyannar Madhu , Anbarasu Kannan

{"title":"Exosomal 4EBP1 promotes head and neck cancer progression via regulating mitochondrial fission","authors":"Iyyannar Madhu , Anbarasu Kannan","doi":"10.1016/j.bbrc.2025.151735","DOIUrl":"10.1016/j.bbrc.2025.151735","url":null,"abstract":"<div><div>Head and neck cancer (HNC) is the sixth most common cancer around the globe with raised incidence and mortality. Despite the advancement in diagnostic and therapeutic approaches the burden of HNC has not reduced. Therefore, investigation on key molecular mechanisms that contributes to the progression of HNC is required to identify promising therapeutic targets. Exosomes are nanosized vesicles and recently emerged as a carrier of tumorigenic proteins essential for cancer progression. However, the role of exosomal proteins in HNC progression remains largely unclear. Eukaryotic Initiation Factor 4E-Binding protein 1 (4EBP1) regulates the protein synthesis and plays a crucial role in the progression of different forms of cancer. Our current study revealed that 4EBP1 is carried in human serum exosomes and upregulated in HNC serum exosomes than healthy controls (HC) and we observed that coculturing the 4EBP1 upregulated HNC serum exosomes (HNC Exo) promoted the growth and migration of HEp-2 cells. Further, we examined the underlying mechanism by knockdown of 4EBP1 in HEp-2 cells (4EBP1 KD). Our results showed that knockdown of 4EBP1 have suppressed the migration and progression of cancer cells. Mechanistically, knockdown of 4EBP1 downregulated mitochondrial fission modulators DRP1 and FIS1 and attenuated the migration of HNC cancer cells by suppressing TGFβ and upregulating PTEN. Together our findings suggest that 4EBP1 is upregulated in circulating exosomes and promotes HNC progression via modulating mitochondrial fission and could be a potential therapeutic target for HNC.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151735"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics analysis reveals the diagnostic and therapeutic value of the centrosome replication-related gene SPICE1 in keloid

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151743

Yin Wang , Zhengguo Xia , Wengting Wang , Jingsong Zhang , Chao Hu , Fan Wang , Jun Wang , Xiaojing Li

{"title":"Bioinformatics analysis reveals the diagnostic and therapeutic value of the centrosome replication-related gene SPICE1 in keloid","authors":"Yin Wang , Zhengguo Xia , Wengting Wang , Jingsong Zhang , Chao Hu , Fan Wang , Jun Wang , Xiaojing Li","doi":"10.1016/j.bbrc.2025.151743","DOIUrl":"10.1016/j.bbrc.2025.151743","url":null,"abstract":"<div><div>Keloids are the abnormal accumulation of collagen in the dermis, which leads to the formation of raised fibrous tissue at the site of injury and the development of a hard scar. The underlying pathological mechanisms associated with keloids have not been fully elucidated. In this study, two GEO datasets (GSE44270 and GSE7890) were employed alongside two machine learning algorithms, Support Vector Machine-Recursive Feature Elimination (SVM-RFE) and Random Forest, to identify diagnostic biomarkers associated with centrosome replication. Following identifying these biomarkers, researchers conducted a functional enrichment analysis to elucidate their biological significance and constructed a gene regulatory network to map their interactions. Furthermore, the study investigated the role of SPICE1 in keloid formation, utilizing a mouse model to explore its potential implications in this pathological process. Researchers identified ten diagnostic markers associated with centrosome replication, among which SPICE1 was significantly upregulated in keloid tissues and fibroblasts. In vivo experiments further demonstrated that the overexpression of SPICE1 promotes keloid formation. Additionally, functional enrichment analysis revealed a connection between these markers and immune cells, suggesting that the immune system may play a crucial role in the development of keloids. This study indicates that SPICE1 is a potential diagnostic marker and therapeutic target for keloids. It offers new insights into the pathological mechanisms of keloids and the development of novel treatments.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151743"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long noncoding RNA IDI2-AS1 modulates the expression of interleukin 5 in human cells

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151733

Ryuki Endo, Miyu Kurisu, Hidenori Tani

{"title":"Long noncoding RNA IDI2-AS1 modulates the expression of interleukin 5 in human cells","authors":"Ryuki Endo, Miyu Kurisu, Hidenori Tani","doi":"10.1016/j.bbrc.2025.151733","DOIUrl":"10.1016/j.bbrc.2025.151733","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) have emerged as critical regulators of gene expression, influencing a wide range of biological processes. In this study, we investigate the regulatory role of the lncRNA IDI2-AS1 in the immune response. Our previous observations demonstrated that IDI2-AS1 expression is downregulated in A549 cells upon exposure to lipopolysaccharide (LPS) and poly I:C, which mimic bacterial and viral infections, respectively. Here, we analyzed the expression changes of 13 immune response genes following siRNA-mediated knockdown of IDI2-AS1 in A549 cells. Notably, our results revealed a significant and selective upregulation of interleukin 5 (IL5) mRNA expression, which increased approximately 60-fold, along with a corresponding ∼70-fold increase in IL5 protein levels. These findings suggest a novel regulatory mechanism in which IDI2-AS1 functions as a suppressor of IL5 expression under normal conditions. During simulated bacterial or viral infections, the downregulation of IDI2-AS1 appears to initiate a rapid and robust increase in IL5 expression. Given the pivotal role of IL5 in allergic inflammation and eosinophil regulation, the IDI2-AS1-IL5 axis may represent an important pathway in the immune response to pathogenic challenges. This study provides new insights into the intricate interplay between lncRNAs and cytokine gene regulation in innate immunity, potentially offering novel therapeutic targets for immune-related disorders.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151733"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, molecular docking, and antiproliferative activity studies of bromine bearing Schiff bases

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151739

Halis Karatas , Burçin Turkmenoglu , Zülbiye Kokbudak , Senem Akkoc

{"title":"Synthesis, molecular docking, and antiproliferative activity studies of bromine bearing Schiff bases","authors":"Halis Karatas , Burçin Turkmenoglu , Zülbiye Kokbudak , Senem Akkoc","doi":"10.1016/j.bbrc.2025.151739","DOIUrl":"10.1016/j.bbrc.2025.151739","url":null,"abstract":"<div><div>Bromine-bearing compounds are found in a variety of natural products and synthetic drugs and exhibit a variety of biological activities. They often display particular properties such as enhanced stability, improved lipophilicity, and larger molecular interactions, making them valuable in drug design and development. In this study, three bromine-bearing Schiff bases were synthesized, characterized, and tested in lung (A549) and colon (DLD-1) cancer cell lines for 72 h incubation time. The results demonstrated that compounds had antiproliferative activity in screened cell lines. The compounds were also tested on a healthy embryonic kidney cell line (HEK-293T) to evaluate their toxic effects on non-cancerous cells. <em>In vitro</em> results of compounds interacting with the most suitable target were confirmed by <em>in silico</em> approaches. All bromine-containing compounds were interacted with the receptor tyrosine kinase FLT3 target via molecular docking. From the interaction results of the 6JQR crystal structure with 2-Br, the docking score value was calculated as −8.178 kcal/mol, and this value was determined to be better than the docking score (−7.269 kcal/mol) value of gilteritinib. Pharmacokinetics, toxicity properties and Lipinski violations of the compounds were estimated by ADMET and were calculated to be in the range of recommended values. Molecules demonstrated limited efficacy against A549 cells line, however; they exhibited relatively greater effectiveness against DLD-1 cell line. Notably, the IC<sub>50</sub> value of 2-Br was comparable to that of cisplatin. All molecules exhibited minimal toxicity.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151739"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell surface display of a protein based on a tail-anchored membrane protein 基于尾锚膜蛋白的细胞表面蛋白质展示

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151738

Shinji Sueda, Rima Tsuruga, Takumi Hirakawa, Satoshi Fujii

{"title":"Cell surface display of a protein based on a tail-anchored membrane protein","authors":"Shinji Sueda, Rima Tsuruga, Takumi Hirakawa, Satoshi Fujii","doi":"10.1016/j.bbrc.2025.151738","DOIUrl":"10.1016/j.bbrc.2025.151738","url":null,"abstract":"<div><div>Methods for displaying proteins on the cell surface are widely used in protein-based biotechnology and bioengineering, where target proteins are expressed as fusion constructs with membrane proteins through recombinant DNA technology. In this study, we developed a system for displaying a protein on the cell surface using the transmembrane domain (TMD) of a tail-anchored membrane protein (TA protein). TA proteins have an orientation in the cell membrane such that their C-termini are displayed on the cell surface, which contrasts with that of type I transmembrane proteins that are commonly used as anchoring units. Therefore, by utilizing the TMD of a TA protein as an anchoring unit, desired proteins can be attached to the TMD via their N-termini. This approach is advantageous for displaying proteins whose C-terminal regions play important roles in their activity. In this study, we chose the inner nuclear membrane protein emerin as a TA protein and constructed expression systems in mammalian cells for a series of fusion proteins based on deleted forms of emerin. We found that utilizing emerin that lacks 210 residues from the N-terminus as a TMD allowed efficient translocation of the fusion protein to the plasma membrane, successfully displaying its target protein portion on the cell surface. Thus, our system serves as an effective method for protein display, enhancing the applicability of cell surface display technology based on transmembrane proteins.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151738"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-frequency, mild-gradient chronic intermittent hypoxia still induces liver fibrogenesis in mice on a high-fat diet

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151744

Junpei Kudo, Haruka Hirono, Shogo Ohkoshi

{"title":"Low-frequency, mild-gradient chronic intermittent hypoxia still induces liver fibrogenesis in mice on a high-fat diet","authors":"Junpei Kudo, Haruka Hirono, Shogo Ohkoshi","doi":"10.1016/j.bbrc.2025.151744","DOIUrl":"10.1016/j.bbrc.2025.151744","url":null,"abstract":"<div><div>The fibrogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD) may progress when complicated by obstructive sleep apnea (OSA). Studies using animal models have shown that high-frequency intermittent hypoxia (IH) exposure, which resembles human OSA, accelerates liver fibrosis in fatty liver. This study highlights that low-frequency, mild-gradient intermittent hypoxia (IH) can exacerbate fibrogenesis in fatty liver disease, even without significantly raising markers of liver injury or insulin resistance. Using a mice model on a high-fat diet (HFD), we found that while routine liver tests (e.g., ALT, AST) and cholesterol levels remained comparable between HFD mice exposed to room air (RA) versus those exposed to chronic intermittent hypoxia (CIH), indicators of liver fibrosis and oxidative stress were elevated in the latter group. This suggests that even low-frequency, mild-gradient IH can increase oxidative stress and fibrotic activity within the liver, primarily through the upregulation of specific markers like ICAM-1 and osteopontin (OPN), which may play a role CIH-induced liver inflammation and fibrosis in fatty liver. In conclusion. Our study further notes that these hypoxia-related changes occurred without significantly worsening systemic insulin resistance, focusing attention on localized liver impacts rather than global metabolic disruptions. The findings underscore the potential role of oxidative stress and specific cytokines in the progression of liver fibrosis in MASLD, especially when complicated by conditions that introduce IH.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151744"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chain-length preference of trans-acting enoylreductases involved in the biosynthesis of fungal polyhydroxy polyketides 参与真菌多羟基聚酮生物合成的反式作用烯基还原酶的链长偏好

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151737

Yuichiro Takekawa , Junya Takino , Shusuke Sato , Hideaki Oikawa , Toyoyuki Ose , Atsushi Minami

{"title":"Chain-length preference of trans-acting enoylreductases involved in the biosynthesis of fungal polyhydroxy polyketides","authors":"Yuichiro Takekawa , Junya Takino , Shusuke Sato , Hideaki Oikawa , Toyoyuki Ose , Atsushi Minami","doi":"10.1016/j.bbrc.2025.151737","DOIUrl":"10.1016/j.bbrc.2025.151737","url":null,"abstract":"<div><div>Fungal polyketides are diverse natural products synthesized by iterative polyketide synthases (i-PKSs) and modified by enzymes such as <em>trans</em>-acting enoylreductases (<em>trans</em>-ERs). In this study, we investigated PhiaB and PhomB, <em>trans</em>-ERs involved in the biosynthesis of polyhydroxy polyketides, phialotides, and phomenoic acids. In vitro assays using substrate analogs revealed distinct chain-length preferences. X-ray structural analysis of PhiaB revealed distinct <em>N</em>-terminal, central, and <em>C</em>-terminal regions. The importance of the central region, which possesses a canonical Rossmann fold for cofactor recognition, was further supported by biosynthetic refactoring using a chimeric enzyme. Docking studies revealed key amino acid residues that may be involved in substrate/cofactor recognition. These findings advance our understanding of <em>trans</em>-ER function, providing opportunities for the synthesis of structurally different polyhydroxy polyketides by genetic engineering of polyhydroxy polyketide biosynthesis.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151737"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative readout of methionine residue solvent accessibility in E. coli cells using radiolytic hydroxyl radical labeling and mass spectrometry 利用放射性羟基自由基标记和质谱法定量测定大肠杆菌细胞中蛋氨酸残渣的溶剂可及性

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-04-01 DOI: 10.1016/j.bbrc.2025.151745

Ezaz Ahmed , Rohit Jain , Daniela Schlatzer , Filipa Blasco Tavares Pereira Lopes , Janna Kiselar , David T. Lodowski , Mark R. Chance , Erik R. Farquhar

{"title":"Quantitative readout of methionine residue solvent accessibility in E. coli cells using radiolytic hydroxyl radical labeling and mass spectrometry","authors":"Ezaz Ahmed , Rohit Jain , Daniela Schlatzer , Filipa Blasco Tavares Pereira Lopes , Janna Kiselar , David T. Lodowski , Mark R. Chance , Erik R. Farquhar","doi":"10.1016/j.bbrc.2025.151745","DOIUrl":"10.1016/j.bbrc.2025.151745","url":null,"abstract":"<div><div>Reactive oxygen species play a crucial role in cellular processes, but their effects on protein structure and function <em>in vivo</em> remain challenging to study. Here, we present an approach using synchrotron-based X-ray footprinting methods to probe protein structure, via quantitative LC-coupled mass spectrometry of methionine oxidation (MSOx) in live <em>E. coli</em>. A label-free proteomic analysis identified 2104 proteins from <em>E. coli</em>, with 465 proteins exhibiting MSOx modifications distributed across multiple cellular compartments. Changes in MSOx modification with increasing X-ray dose revealed a correlation between rates of modification and solvent-accessible surface area <em>in vivo</em> for selected proteins responsive to exposure, providing a direct probe of protein structure and its conformational plasticity in the cell. The approach developed here offers a unique in-cell quantitative readout of methionine oxidation and solvent accessibility through radiolytic hydroxyl radical labeling. With this method, the landscape of methionine oxidation in <em>E. coli</em> can be mapped, providing insights into protein behavior under oxidative stress. It represents a first step in developing radiolysis and <em>E. coli</em> as platforms for <em>in vivo</em> protein structure assessment. The potential applications in drug discovery, protein engineering, and systems biology of protein conformations are considerable.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"762 ","pages":"Article 151745"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chronic corticosterone administration on acute stress-mediated gene expression in the cortex and hippocampus of male mice 慢性皮质酮对雄性小鼠皮质和海马急性应激介导基因表达的影响

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-31 DOI: 10.1016/j.bbrc.2025.151729

Hirono Shiraki, Eri Segi-Nishida, Kanzo Suzuki

{"title":"Effect of chronic corticosterone administration on acute stress-mediated gene expression in the cortex and hippocampus of male mice","authors":"Hirono Shiraki, Eri Segi-Nishida, Kanzo Suzuki","doi":"10.1016/j.bbrc.2025.151729","DOIUrl":"10.1016/j.bbrc.2025.151729","url":null,"abstract":"<div><div>Corticosterone plays an important role in the stress response, physiological regulation, and development of stress-related psychiatric disorders. Although several studies have demonstrated that chronic corticosterone induces anxiety- or depressive-related behaviors in mice, it remains unclear whether chronic corticosterone administration affects gene expression in the brain during the stress response. This study investigated whether chronic corticosterone administration has a significant effect on stress-related gene expression in the brain. Therefore, mice were chronically treated with corticosterone in drinking water and gene expression was analyzed by quantitative PCR (qPCR). Moreover, restraint stress was acutely applied as a novel stressor in mice chronically treated with corticosterone in the cortex and hippocampus. We initially found that chronic corticosterone administration altered glucocorticoid signaling-mediated gene expression, such as FK506 binding protein 5 (<em>Fkbp5</em>) and glucocorticoid-inducible kinase 1 (<em>Sgk1</em>), in the cortex and hippocampus of mice. Next, we found that restraint stress exposure elevated <em>Fkbp5</em> expression in the vehicle group; however, chronic corticosterone administration occluded further induction of <em>Fkbp5</em> expression after restraint stress exposure. In addition, pro-inflammatory cytokines tumor necrosis factor α (<em>Tnfa</em>) and interleukin-1β (<em>Il1b</em>) mRNA expression in the cortex and hippocampus were remarkably enhanced by restraint stress in corticosterone-treated mice, but not in the vehicle group. Collectively, our results demonstrated that chronic corticosterone administration modulates glucocorticoid signaling and uncovered the robust induction of pro-inflammatory cytokines after restraint stress exposure in chronically corticosterone-treated mice. These mechanisms may be involved in the molecular basis for the onset of stress-related mental illnesses.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"762 ","pages":"Article 151729"},"PeriodicalIF":2.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium exposure induces Leydig cell injury via necroptosis caused by oxidative stress and TNF-α/TNFR1 signaling 镉暴露通过氧化应激和TNF-α/TNFR1信号通路引起的坏死坏死诱导间质细胞损伤

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2025-03-31 DOI: 10.1016/j.bbrc.2025.151717

Xiaoping Zheng , Yaohui Sun , Jinhua Wang , Yinghao Yin , Zitaiyu Li , Biao Liu , Hongji Hu , Jiarong Xu , Yingbo Dai , Yashpal S. Kanwar , Yuxin Tang

{"title":"Cadmium exposure induces Leydig cell injury via necroptosis caused by oxidative stress and TNF-α/TNFR1 signaling","authors":"Xiaoping Zheng , Yaohui Sun , Jinhua Wang , Yinghao Yin , Zitaiyu Li , Biao Liu , Hongji Hu , Jiarong Xu , Yingbo Dai , Yashpal S. Kanwar , Yuxin Tang","doi":"10.1016/j.bbrc.2025.151717","DOIUrl":"10.1016/j.bbrc.2025.151717","url":null,"abstract":"<div><div>Cadmium, a ubiquitous environmental pollutant, has been linked to testicular damage, primarily through mechanisms such as oxidative stress and various forms of programmed cell death. Despite extensive studies on its toxic effects, the specific role of necroptosis in cadmium-induced reproductive toxicity remains unclear. In this study, we provide critical insights into how cadmium triggers necroptosis in Leydig cells, leading to testicular dysfunction. Using both in vitro and in vivo models, we demonstrated that cadmium exposure induces necroptotic cell death in Leydig cells, with significant involvement of the TNF-α/TNFR1 signaling pathway and reactive oxygen species (ROS) generation. Co-treatment with Nec-1, a specific necroptosis inhibitor, significantly reduced elevated ROS levels and suppressed TNF-α/TNFR1-induced necroptotic cell death, suggesting that ROS and the TNF-α/TNFR1 signaling pathway contribute to necroptosis activation in cadmium-induced Leydig cell injury. In conclusion, we demonstrate that necroptosis is a key driver of cadmium-induced testicular damage, suggesting that targeting necroptosis could offer novel therapeutic strategies for mitigating reproductive toxicity caused by heavy metals.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"761 ","pages":"Article 151717"},"PeriodicalIF":2.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0