Biocontrol science最新文献_第4页

Study Elucidating Disinfection at Nursery Schools in Ibaraki, Japan. 日本茨城市幼儿园消毒情况研究

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.37

Michiko Nohara, Tamie Sugawara, Junko Kurita, Naomi Sakurai, Miho Umezawa, Noriko Nagata, Yasushi Ohkusa

{"title":"Study Elucidating Disinfection at Nursery Schools in Ibaraki, Japan.","authors":"Michiko Nohara, Tamie Sugawara, Junko Kurita, Naomi Sakurai, Miho Umezawa, Noriko Nagata, Yasushi Ohkusa","doi":"10.4265/bio.26.37","DOIUrl":"https://doi.org/10.4265/bio.26.37","url":null,"abstract":"Control of infectious diseases requires switching from usual hygiene such as water wiping and cleaning, to control measures including appropriate and aggressive disinfection using 70% alcohol and/or hypochlorous acid depending on the pathogen. Nevertheless, some nursery schools might not understand or select proper disinfectant methods. A survey was administered by the local government of Ibaraki prefecture, Japan in January 2018 to all 456 nursery schools in Ibaraki prefecture. The surveyed items were the number of nursery teachers, usual manual hygiene among children, usual disinfection for lavatories, diaper changing spaces, tables used by children for meals, and classroom floors when there was no vomiting in the facilities and no outbreak in surrounding area. Moreover, it asked about disinfection procedures if children vomited during a community outbreak of gastroenteritis infection. We defined proper use for usual disinfection of a lavatory or diaper changing space as chlorine-based disinfectant including hypochlorous acid or 70% alcohol according to guidelines. Overall, 403 nursery schools responded to the survey. All nursery schools implemented usual hand hygiene. Association between proper disinfection and the size of nursery schools was not significant. Moreover, association between proper disinfection and nursery schools with nurse presence was not found to be significant.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 1","pages":"37-41"},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25475368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Contamination of Rubber Ducks Floating in Bathtubs of Bathing Facilities, and an Evaluation of Their Washing Methods. 游泳设施浴缸中漂浮橡皮鸭的微生物污染及清洗方法评价。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.187

Akiko Edagawa, Norihiro Matsuda, Toru Ogura, Kenichi Uezono, Shinji Izumiyama, Akira Fujii

引用次数: 0

Two Potential Probiotic Bacillus with Proteolytic Activity to Dietary Protein from Adult Feces. 两种对成虫粪便中蛋白质具有潜在水解活性的益生菌。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.221

Haiqi Wang, Xichun Zhang, Zhaohua Chen, Gengxin Hao, Guiling Li

引用次数: 2

Inhibitory Effects of Escherichia coli on the Formation and Development of Staphylococcus epidermidis Biofilm. 大肠杆菌对表皮葡萄球菌生物膜形成和发育的抑制作用。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.113

Han-Min Ohn, Tamaki Mizuno, Shin-Ichi Miyoshi

引用次数: 1

Examination of conditions for regular internal quality control in identification of microorganisms using MALDI-TOF MS. MALDI-TOF质谱法鉴定微生物常规内部质量控制条件的探讨。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.99

Kazuyuki Sogawa, Makiko Kiyosuke, Kanako Hattori, Syota Murata, Tomohisa Watari, Koji Kusaba, Yuji Saeki, Kazuki Horiuchi, Kazunari Yasuda, Kohei Uechi, Hajime Okumura, Sachie Sekiguchi, Azumi Fujinaga, Masami Murakami, Tomohiro Nakayama

引用次数: 1

Enterococcus faecium CMUL1216 an Immunobiotic Strain with a Potential Application in Animal Sector. 粪肠球菌CMUL1216是一种具有潜在应用价值的免疫生物菌株。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.75

Imad Al Kassaa, Samah Mechmchani, Mazen Zaylaa, Mohamad Bachar Ismail, Khaled El Omari, Fouad Dabboussi, Monzer Hamze

{"title":"Enterococcus faecium CMUL1216 an Immunobiotic Strain with a Potential Application in Animal Sector.","authors":"Imad Al Kassaa, Samah Mechmchani, Mazen Zaylaa, Mohamad Bachar Ismail, Khaled El Omari, Fouad Dabboussi, Monzer Hamze","doi":"10.4265/bio.26.75","DOIUrl":"https://doi.org/10.4265/bio.26.75","url":null,"abstract":"Antibiotic misuse in the animal sector is the first cause of the emergence and spreading of MDR bacteria. Prevention of infectious diseases and enhancement of animal growth are the main effects of antibiotics that push farmers and veterinarians to use this molecule in animal farms. Thus, the use of alternative solutions such as natural antimicrobial substances as well as probiotic strains is a crucial need in this sector. Enterococcus faecium CMUL1216 was isolated from healthy human baby's feces. This strain was assessed in vitro for probiotic properties including activity against many pathogens isolated from animal, human, and soil samples. CMUL1216 strain exhibits good antimicrobial activity against indicator pathogens in both planktonic and biofilm forms. In addition, CMUL1216 strain showed a strong biofilm formation. Furthermore, CMUL1216 exhibits a good anti-inflammatory effect by inducing the secretion of IL-10 in vitro. Moreover, this strain did not show any pathogenic characteristics such as hemolytic effect, presence of virulence genes as well as susceptibility to the majority of antibiotic families. E. faecium CMUL1216 could be a good candidate to be used a probiotic strain in the animal sector in order to maintain animal health and therefore reduce antibiotic resistance caused by the excessive use in this sector.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 2","pages":"75-84"},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38997812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of Culturable Bacteria from Tomato Seeds on Media Semi-selective for the Canker Pathogen. 番茄种子可培养菌在半选择性培养基上对溃疡病病原的检测。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.61

Nobutaka Someya, Masaharu Kubota, Tomohiro Morohoshi, Yasuhiro Inoue

引用次数: 1

Environmental survey of Methicillin-Resistant Staphylococci in a Hospital in Japan. 日本某医院耐甲氧西林葡萄球菌环境调查。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.137

Akari Watanabe, Tokiko Watanabe, Susumu Kokeguchi, Yumiko Yamamoto, Osamu Matsushita, Kenji Yokota

{"title":"Environmental survey of Methicillin-Resistant Staphylococci in a Hospital in Japan.","authors":"Akari Watanabe, Tokiko Watanabe, Susumu Kokeguchi, Yumiko Yamamoto, Osamu Matsushita, Kenji Yokota","doi":"10.4265/bio.26.137","DOIUrl":"https://doi.org/10.4265/bio.26.137","url":null,"abstract":"We examined the hospital-wide incidence of methicillin-resistant Staphylococcus contamination in a hospital environment to predict the risk of the nosocomial spread of infection. Samples were also taken different surfaces and medical equipment in a general hospital ward and a staff station. The isolates were identified bacterial strains and analyzed by PCR for detection of the mecA gene and staphylococcal cassette chromosome mec (SCCmec) types (I-V). Overall, out of 146 isolates that were screened, 15.7% of the samples in the hospital wards were contaminated with Staphylococcus aureus and 74.7% were isolated with coagulase-negative Staphylococci (CNS). The methicillin-resistant mecA gene was detected in all oxacillin-resistant S. aureus, and 89% of oxacillin-resistant CNS was identified as methicillin-resistant S. aureus (MRSA) and MRCNS respectively. All S. aureus and CNS from the hospital wards with MRSA patients were detected as MRSA and MRCNS. A widespread distribution of MRSA and MRCNS was detected in the Cuff. The majority of the MRSA and MRCNS isolates in this study were SCCmec type V, which are a community-acquired infection type. The increased incidence and prevalence of community-acquired MRSA and MRCNS, as well as hospital-acquired MRSA, should be recognized as serious healthcare problems.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 3","pages":"137-145"},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39444887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spore Heat Resistance and Growth Ability at Refrigeration Temperatures of Bacillus spp. and Paenibacillus spp. 芽孢杆菌和拟芽孢杆菌的耐热性及其在冷藏温度下的生长能力。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.147

Tetsuya Kobayashi, Takanori Azuma, Daisuke Yasokawa, Shogo Yamaki, Koji Yamazaki

{"title":"Spore Heat Resistance and Growth Ability at Refrigeration Temperatures of Bacillus spp. and Paenibacillus spp.","authors":"Tetsuya Kobayashi, Takanori Azuma, Daisuke Yasokawa, Shogo Yamaki, Koji Yamazaki","doi":"10.4265/bio.26.147","DOIUrl":"https://doi.org/10.4265/bio.26.147","url":null,"abstract":"In this study, spore heat resistance and growth ability at refrigeration temperatures of Bacillus spp. and Paenibacillus spp. were determined. The spore D90°C of 67.6% (23 of 34 strains) of Bacillus and 73.9% (17 of 23 strains) of Paenibacillus was less than 15 min. The growth abilities of both genera were equivalent at 10°C. However, 71.1% (32 of 45 strains) of Paenibacillus and only 6.3% (3 of 48 strains) of Bacillus cereus group could grow at 4°C. Eight B. cereus strains formed spores with higher heat resistance compared to the other Bacillus strains assessed; however, they did not grow at tempreratures below 10°C. Conversely, four Paenibacillus strains formed spores with heat resistance equivalent to that of the eight B. cereus strains and grew at 6°C or lower. In particular, Paenibacillus sp. JCM13343 formed the highest heat-resistant spores (D90°C = 136.1 min) and grew well at 4°C. These results indicate that Paenibacillus can grow in processed foods during refrigerated storage and has the potential to cause spoilage as well as Bacillus. Therefore, Paenibacillus should be considered as one of the targets for microbiological control in refrigerated processed foods.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 3","pages":"147-155"},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39444888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes. 从埃及患病尼罗罗非鱼(Oreochromis niloticus)中分离出的嗜水气单胞菌及其毒力相关基因的测序

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2021-01-01 DOI: 10.4265/bio.26.167

Amany Saleh, Rasha Elkenany, Gamal Younis

{"title":"Virulent and Multiple Antimicrobial Resistance Aeromonas hydrophila Isolated from Diseased Nile Tilapia Fish (Oreochromis niloticus) in Egypt with Sequencing of Some Virulence-Associated Genes.","authors":"Amany Saleh, Rasha Elkenany, Gamal Younis","doi":"10.4265/bio.26.167","DOIUrl":"https://doi.org/10.4265/bio.26.167","url":null,"abstract":"Aeromonas hydrophila is a major waterborne pathogen, which induces various diseases in freshwater fish with the capability for zoonotic potential. This study was applied to investigate the prevalence of A. hydrophila in diseased Nile tilapia fish, genetic characterization of the virulence encoding genes (act, aerA, alt, and ast genes), and antibiotic susceptibility. Out of the 500 diseased Nile tilapia fish samples, 70% (350/500) Aeromonas species were isolated. From which 53.4% (187/350) of Aeromonas hydrophila strains were identified. A. hydrophila was detected in kidneys, followed by liver, spleen, intestine, and gills. The results of virulotyping displayed the presence of act, and aerA genes in a high percentage of 40%, followed by alt gene (30%), but ast gene was not detected (0%) in A. hydrophila strains. Based on DNA sequence analysis of three virulence associated-genes (act, aerA, and alt genes), the phylogenetic tree showed the genetic relationship with related species. Finally, the antibiotic susceptibility tests revealed high resistance toward chloramphenicol (67.4%), followed by amikacin (51.9%) and gentamicin (47.1%), whereas a high sensitivity was exhibited toward meropenem (90.9%), followed by ciprofloxacin (84.2%), amoxicillin-clavulanic acid (73.3%) and trimethoprim-sulfamethoxazole (64.2%). The multidrug-resistant A. hydrophila strains were observed in 69.0% of strains with six resistance patterns.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"26 3","pages":"167-176"},"PeriodicalIF":1.2,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39443302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10