Detection of Culturable Bacteria from Tomato Seeds on Media Semi-selective for the Canker Pathogen.

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2021-01-01 DOI:10.4265/bio.26.61

Nobutaka Someya, Masaharu Kubota, Tomohiro Morohoshi, Yasuhiro Inoue

引用次数: 1

Abstract

Culturable bacteria were isolated from tomato seeds using media selective for the canker pathogen Clavibacter michiganensis subsp. michiganensis. Clustering analysis (>99% identity) revealed the presence of 16 operational taxonomic units (OTUs) among isolates detected on semi-selective media. Three OTUs belonged to the phylum Actinobacteria, including those of Micrococcus and Dermacoccus, and 13 OTUs belonged to the phylum Firmicutes, including Bacillus and related genera. These Gram-positive endophytic bacteria have the potential to provide false-positive results in seed health tests using media considered semi-selective for the cancer pathogen.

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番茄种子可培养菌在半选择性培养基上对溃疡病病原的检测。

采用选择性培养基从番茄种子中分离出可培养的溃疡病病原菌密歇根克拉维杆菌亚种。michiganensis。聚类分析显示，在半选择性培养基上检测到的分离株中存在16个可操作分类单位(otu)。3个otu属于放线菌门，包括微球菌和皮球菌，13个otu属于厚壁菌门，包括芽孢杆菌及其相关属。这些革兰氏阳性内生细菌有可能在使用被认为是癌症病原体半选择性培养基的种子健康测试中提供假阳性结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.