Clinical molecular pathology最新文献_第8页

Tumour heterogeneity: a problem in biopsy assessment of the proliferation index of oesophageal adenocarcinomas. 肿瘤异质性:食管癌增殖指数活检评估中的一个问题。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m61

K Jenner, S J Darnton, L Billingham, A T Warfield, H R Matthews

引用次数: 4

Expression of type III collagen mRNA in renal biopsy specimens of patients with idiopathic membranous glomerulonephritis. 特发性膜性肾小球肾炎患者肾活检标本中III型胶原mRNA的表达。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m40

M S Razzaque, T Taguchi

{"title":"Expression of type III collagen mRNA in renal biopsy specimens of patients with idiopathic membranous glomerulonephritis.","authors":"M S Razzaque, T Taguchi","doi":"10.1136/mp.49.1.m40","DOIUrl":"https://doi.org/10.1136/mp.49.1.m40","url":null,"abstract":"Aim-To investigate the distribution of type III collagen in membranous glomerulonephritis (MGN); to identify the cells responsible for the synthesis of alpha1 (III) mRNA.method-The distribution of type III collagen was studied by immunohistochemistry in 10 renal biopsy specimens, histologically diagnosed as MGN, and five control renal tissue samples obtained at surgery. Synthesis of alpha1 (III) mRNA was detected by non-radioactive in situ hybridisation.Results-On immunohistochemistry, type III collagen was not observed in the control glomeruli, but was present focally in the glomeruli in samples from patients with MGN. No specific hybridisation signal for alpha1 (III) mRNA was found in the control glomeruli on non-radioactive in situ hybridisation. By contrast, positive signals for alpha1 (III) chain mRNA were detected in glomerular epithelial cells and mesangial cells in MGN tissue samples.Conclusion-These data suggest that additional synthesis of type III collagen by intraglomerular cells contributes to the changes in the glomerular basement membrane characteristic of MGN.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m40","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26021479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Diagnosis, epidemiology and pathogenesis of bacterial infections in the molecular era. 分子时代细菌感染的诊断、流行病学和发病机制。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m1

S Sethi, T F Murphy, K L Klingman

引用次数: 4

Complete structural characterisation of the human aryl hydrocarbon receptor gene. 人类芳烃受体基因的完整结构表征。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m12

P Bennett, D B Ramsden, A C Williams

{"title":"Complete structural characterisation of the human aryl hydrocarbon receptor gene.","authors":"P Bennett, D B Ramsden, A C Williams","doi":"10.1136/mp.49.1.m12","DOIUrl":"https://doi.org/10.1136/mp.49.1.m12","url":null,"abstract":"Aims-To clone and characterise the complete structural gene for the human aryl hydrocarbon receptor (AhR). This gene, located on chromosome 7, encodes a cytosolic receptor protein which, upon activation by various xenobiotic ligands, translocates to the nucleus, where it acts as a specific transcription factor.Methods-Primers, based on the AhR cDNA sequence, were used in conjunction with recently developed long range PCR techniques to amplify contiguous sections of the cognate gene. The amplicons produced were then cloned and characterised. A cDNA probe was also used to screen a human P1 library.Results-Using the cDNA primers, DNA fragments which mapped the entire coding region of the gene were amplified and cloned. All but one of these fragments were amplified directly from human genomic DNA. The remaining fragment was amplified using DNA prepared from a P1 clone as the PCR template. This P1 clone, obtained by screening a human P1 library, also contained the entire Ah locus. Characterisation of amplified and cloned DNA fragments provided sufficient information for the construction of a complete structural map of the gene. This also included 150 base pairs of nucleotide sequence data at all intronic termini.Conclusions-These data indicate that the human AhR gene is about 50 kilobases long and contains 11 exons. The overall intron/exon structure of the human gene is homologous to that of the previously characterised mouse gene; however, it is probably some 20 kilobases larger. These results demonstrate the need for further characterisation and provide the data to facilitate this.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Adhesion of platelets to chemotactically responsive and non-responsive neutrophils. 血小板与化学反应性和非反应性中性粒细胞的粘附。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m17

K B Pastakia, N E Brownson, D A Terle, L Harvath

{"title":"Adhesion of platelets to chemotactically responsive and non-responsive neutrophils.","authors":"K B Pastakia, N E Brownson, D A Terle, L Harvath","doi":"10.1136/mp.49.1.m17","DOIUrl":"https://doi.org/10.1136/mp.49.1.m17","url":null,"abstract":"Aims-To investigate the heterotypic adhesion of unactivated platelets to chemotactically responsive (migrated) and non-responsive (non-migrated) polymorphonuclear neutrophils (PMN).Methods-Platelets and PMN were isolated from autologous, normal blood. Migrated and non-migrated PMN were separated after N-formylmethionyl-leucylphenylalanine (FMLP) stimulation. Platelets were labelled with a fluorescent monoclonal antibody directed against CD41 (GPIIb-IIIa). Platelets (3 x 10(8)/ml) and PMN (3 x 10(6)/ml) were incubated together. Heterotypic cell adhesion was measured in isolated PMN and PMN co-incubated with platelets by flow cytometric analysis of platelet marker fluorescence in PMN gated events. Platelet-PMN adhesion was also visualised by fluorescence microscopy.Results-In studies of isolated PMN, contaminating platelets were bound to 16-34% of unstimulated PMN, 7-22% of stimulated PMN, 2-4% of migrated PMN, and 17-24% of non-migrated PMN. When platelets were co-incubated with migrated or non-migrated PMN, 15-78% of PMN bound one or two platelets.Conclusions-Unactivated platelets adhere to isolated PMN in vitro. Fewer unactivated platelets were adhered to migrated PMN than to non-migrated PMN in isolated PMN preparations. These results indicate that platelets adhering to PMN are removed during PMN migration.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification of Candida species in formalin fixed, paraffin wax embedded oral mucosa by sequencing of ribosomal DNA. 用核糖体DNA测序法鉴定福尔马林固定、石蜡包埋口腔黏膜念珠菌种类。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m23

D W Williams, M J Wilson, M A Lewis, A J Potts

引用次数: 17

Combined non-isotopic in situ hybridisation and indirect immunohistochemical analysis of hormone production in the rat pituitary gland. 结合非同位素原位杂交和间接免疫组织化学分析大鼠垂体激素的产生。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m57

N Sanno, A Matsuno, J Itoh, K Kakimoto, A Teramoto, R Y Osamura

{"title":"Combined non-isotopic in situ hybridisation and indirect immunohistochemical analysis of hormone production in the rat pituitary gland.","authors":"N Sanno, A Matsuno, J Itoh, K Kakimoto, A Teramoto, R Y Osamura","doi":"10.1136/mp.49.1.m57","DOIUrl":"https://doi.org/10.1136/mp.49.1.m57","url":null,"abstract":"An understanding of the intracellular relation between hormonal expression (storage) and gene expression (production) is essential for elucidating the functional status of the individual cells in endocrine tissue such as the pituitary gland. To this end, mRNA expression was visualised by using a combined in situ hybridisation and immunohistochemistry method in routinely processed, formalin fixed, paraffin wax embedded rat pituitaries. mRNA was detected by non-isotopic in situ hybridisation (alkaline phosphatase antialkaline phosphatase method, with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate as substrates). Sections were then stained by using the immunoperoxidase method to demonstrate pituitary hormone expression. The specificity of the combined staining method was confirmed by staining adjacent sections separately. The antigenicity of rat growth hormone and prolactin was adequately preserved following hybridisation. In conclusion, this method is specific, easy to use and permits the determination of the functional status of individual cells.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m57","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26021483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Quantitative Clinical Pathology 定量临床病理

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/MP.49.1.M64-B

J. Crocker

引用次数: 0

Sequence analysis of immunoglobulin variable region genes that encode autoantibodies expressed by lymphomas of mucosa associated lymphoid tissue. 编码粘膜相关淋巴组织淋巴瘤自身抗体的免疫球蛋白可变区基因序列分析。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m29

C J Chapman, D K Dunn-Walters, F K Stevenson, T Hussell, P G Isaacson, J Spencer

{"title":"Sequence analysis of immunoglobulin variable region genes that encode autoantibodies expressed by lymphomas of mucosa associated lymphoid tissue.","authors":"C J Chapman, D K Dunn-Walters, F K Stevenson, T Hussell, P G Isaacson, J Spencer","doi":"10.1136/mp.49.1.m29","DOIUrl":"https://doi.org/10.1136/mp.49.1.m29","url":null,"abstract":"Aim-To determine whether the immunoglobulin genes used by three gastric mucosa associated lymphoid tissue type lymphomas with known autoreactivity are mutated from germline as mutation from germline is an indicator of exposure to a mutational mechanism which characteristically acts on B cells as they undergo a follicle centre response.Methods-Cell lines established from two cases of MALT type lymphoma secrete autoantibodies recognising follicular dendritic cells (one case) and basement membrane (one case). The immunoglobulin heavy chain variable region genes (IgV(H)) and light chain variable region genes (IgV(L)) used by these cell lines, and the IgV(H) genes from a third case recognising human IgG were sequenced.Results-All three cases studied had mutated IgV(H) genes, while the IgV(L) genes were unmutated.Conclusion-The presence of mutations in IgV(H) genes is consistent with the origin of gastric MALT type lymphomas from B cells which have traversed the lymphoid follicle.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Cell Adhesion and Cancer 细胞粘附与癌症

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.M64-a

J. Rockett

引用次数: 0