Clinical molecular pathology最新文献

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m321

A J Freemont, J A Hoyland

引用次数: 17

Inhibitors of collagenase but not of gelatinase reduce cartilage explant proteoglycan breakdown despite only low levels of matrix metalloproteinase activity. 尽管基质金属蛋白酶活性较低，但胶原酶抑制剂而非明胶酶抑制剂可减少软骨外植体蛋白多糖的分解。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m331

C J Brown, S Rahman, A C Morton, C L Beauchamp, H Bramwell, D J Buttle

{"title":"Inhibitors of collagenase but not of gelatinase reduce cartilage explant proteoglycan breakdown despite only low levels of matrix metalloproteinase activity.","authors":"C J Brown, S Rahman, A C Morton, C L Beauchamp, H Bramwell, D J Buttle","doi":"10.1136/mp.49.6.m331","DOIUrl":"https://doi.org/10.1136/mp.49.6.m331","url":null,"abstract":"Aims-To investigate the level of matrix metalloproteinase activity during the time-course of cartilage explant proteoglycan breakdown; to determine the effects of selective small-molecule inhibitors of matrix metalloproteinases on proteoglycan degradation.Methods-The levels of matrix metalloproteinase activity in cartilage explant cultures and conditioned media were monitored by use of a quenched fluorescent substrate. The constants for inhibition of certain matrix metalloproteinases by a series of synthetic inhibitors were determined. Bovine and human cartilage explant cultures were treated with interleukin-1, tumor necrosis factor or retinoic acid and the amount of proteoglycan released into the culture medium in the absence and presence of the inhibitors was quantified. Control experiments, examining the inhibition of other proteinases, and investigating possible toxic or non-specific effects of the inhibitors, were carried out.Results-The profile of inhibition of proteoglycan release suggested the involvement of interstitial collagenase-like, rather than gelatinase- or possibly stromelysin-like, proteinases. No evidence was found for toxic or non-specific mechanisms of inhibition. Very low levels of activity of the known matrix metalloproteinases were present during the time-course of aggrecan breakdown.Conclusions-A novel collagenase-like proteinase(s) may be involved in cartilage proteoglycan breakdown. Gelatinase-type matrix metalloproteinases do not seem to be involved in this process. Specific collagenase inhibitors may be therapeutically efficacious in the treatment of arthritis.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.6.m331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26020855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Scientific deception 科学欺骗

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m368-a

J. A. Harrison

引用次数: 2

Use of proteinase K for RT-PCR of cytokine mRNA in formalin fixed tissue. 用蛋白酶K进行福尔马林固定组织细胞因子mRNA的RT-PCR检测。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m364

G N Davies, I S Bevan, J B Lundemose, H Smith, C Sweet

引用次数: 5

Applications of polymerase chain reaction-single stranded conformational polymorphism to microbiology. 聚合酶链反应-单链构象多态性在微生物学中的应用。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m315

J R Kerr, M D Curran

引用次数: 17

Stress Proteins in Medicine 医学中的应激蛋白

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.M368-b

A. Macario

引用次数: 0

AgNOR clusters as a parameter of cell kinetics in chronic lymphocytic leukaemia. AgNOR簇作为慢性淋巴细胞白血病细胞动力学的参数。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m357

I Lorand-Metze, K Metze

{"title":"AgNOR clusters as a parameter of cell kinetics in chronic lymphocytic leukaemia.","authors":"I Lorand-Metze, K Metze","doi":"10.1136/mp.49.6.m357","DOIUrl":"https://doi.org/10.1136/mp.49.6.m357","url":null,"abstract":"Aims-To study correlations between the pattern of silver stained nucleolar organiser regions (AgNORs) in chronic lymphocytic leukaemia (CLL) and parameters of tumour kinetics. To investigate whether quantitation of the AgNOR pattern can be used to discriminate between patients with stable and progressive disease.Methods-Peripheral blood smears from 48 patients with CLL, classified as having either stable or progressive disease (Rai stage III or IV; bulky lymph nodes or massive splenomegaly; or peripheral lymphocytes >100 x 10(9)/1), were studied. For each patient, total tumour mass (TTM) and for patients undergoing a period of observation without treatment, the TTM duplication time (DT) and the lymphocyte doubling time (LDT) were calculated.Results-Four cell types could be distinguished according to their AgNOR pattern: (1) cells with a single cluster; (2) cells with a single compact nucleolus; (3) cells with two compact nucleoli; and (4) cells with several scattered dots. The percentage of cells with clusters was the AgNOR parameter which correlated best with TTM and LDT. Correlations were also seen between the proportion of cells with clusters and age and haemoglobin concentration. A significant correlation with DT could be detected only when age was kept constant. Linear discriminant analysis revealed that the percentage of cells with clusters was the most important prognostic factor. This alone classified 94% of the patients correctly (jackknive procedure) as either stable or progressive CLL.Conclusions-The percentage of circulating lymphocytes with clusters of AgNORs can be used as a parameter of tumour kinetics in CLL and helps to discriminate between patients with stable and progressive disease. For practical purposes, a value of more than 13% of cells with clusters is suggestive of progressive disease.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.6.m357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26020571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Significantly reduced expression of the proteoglycan decorin in Alzheimer's disease fibroblasts. 显著降低蛋白聚糖decorin在阿尔茨海默病成纤维细胞中的表达。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m351

E Brandan, F Melo, M García, M Contreras

{"title":"Significantly reduced expression of the proteoglycan decorin in Alzheimer's disease fibroblasts.","authors":"E Brandan, F Melo, M García, M Contreras","doi":"10.1136/mp.49.6.m351","DOIUrl":"https://doi.org/10.1136/mp.49.6.m351","url":null,"abstract":"Aims-To investigate whether proteoglycan synthesis is altered in skin fibroblasts in patients with Alzheimer's disease compared with normal subjects.Methods-Cell lines obtained from donors with Alzheimer's disease and healthy controls were incubated with radioactive sulphate. The proteoglycans synthesised were determined and analysed by chromatographic, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and glycosaminoglycans-lyase treatment. The amount of decorin synthesised by each cell line was quantified using western blot analysis. Transcripts for human decorin were determined using northern blot analysis.Results-No significant changes in total sulphate incorporation and glycos-aminoglycan (GAG) composition were detected in the incubation media of these cells. However, chromatographic and SDS-PAGE analysis of the proteoglycans secreted by the cell lines showed that a dermatan sulphate proteoglycan of 150-125 kilodaltons was substantially reduced in Alzheimer's disease fibroblasts. The molecular characteristics of this proteoglycan correspond to decorin. Western blot analysis indicated that decorin was reduced in Alzheimer's disease incubation medium compared with normal medium. Northern blotting indicated that in Alzheimer's disease fibroblasts decorin transcripts were significantly reduced compared with normal fibroblasts. Glypican concentrations, a cell surface heparan sulphate proteoglycan, remained the same.Conclusions-These results strongly suggest that the expression and synthesis of decorin is affected in Alzheimer's disease skin fibroblasts.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.6.m351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26020858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Development of a rapid DNA screening procedure for the Factor V Leiden mutation. 因子V Leiden突变的快速DNA筛选程序的发展。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m361

G A Scobie, S T Ho, G Dolan, N A Kalsheker

引用次数: 10

Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition. 基于生物素化酪胺沉积的原位杂交信号扩增检测人乳头瘤病毒。

Clinical molecular pathology Pub Date : 1996-12-01 DOI: 10.1136/mp.49.6.m340

P J Poddighe, J Bulten, H M Kerstens, J C Robben, W J Melchers, A G Hanselaar

{"title":"Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition.","authors":"P J Poddighe, J Bulten, H M Kerstens, J C Robben, W J Melchers, A G Hanselaar","doi":"10.1136/mp.49.6.m340","DOIUrl":"https://doi.org/10.1136/mp.49.6.m340","url":null,"abstract":"Aim-To describe a method for amplifying human papilloma virus (HPV) in situ hybridisation (ISH) signals.Methods-Three human cervical cell lines, namely CaSKi, HeLa and SiHa, containing different copy numbers of integrated HPV DNA were studied. Following ISH, catalysed reporter deposition (CARD), based on the deposition of biotinylated tyramine at the location of the DNA probe, was used to amplify the ISH signal.Results-Using CARD-ISH, one to three HPV type 16 copies were detected in situ both in cell suspensions and paraffin wax sections of SiHa cells. CARD-ISH can also be used to detect oncogenic HPV DNA sequences, such as HPV types 16 and 18, in routinely processed formalin fixed, paraffin wax embedded cervical specimens.Conclusions-CARD-ISH is a fast and highly sensitive ISH method for the routine detection of low copy number HPV DNA sequences in cervical cell lines and routinely processed tissue sections. Application of this technology also enables the routine detection and cellular localisation of other viral DNA sequences present at copy numbers below the detection limit of conventional ISH methods.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.6.m340","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26020856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14