Clinical molecular pathology最新文献_第7页

The genetics of inherited colon cancer. 遗传性结肠癌的遗传学。

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m65

Y Wallis, F Macdonald

引用次数: 3

p53 gene alterations and protein accumulation in colorectal cancer. 结直肠癌中P53基因改变与蛋白积累。

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m85

R Bertorelle, G Esposito, C Belluco, L Bonaldi, A Del Mistro, D Nitti, M Lise, L Chieco-Bianchi

{"title":"p53 gene alterations and protein accumulation in colorectal cancer.","authors":"R Bertorelle, G Esposito, C Belluco, L Bonaldi, A Del Mistro, D Nitti, M Lise, L Chieco-Bianchi","doi":"10.1136/mp.49.2.m85","DOIUrl":"https://doi.org/10.1136/mp.49.2.m85","url":null,"abstract":"Aim-To correlate immunohistochemical staining with single strand conformation polymorphism (SSCP) analysis of the p53 gene in colorectal cancer in order to understand how the findings provided by the two techniques complement each other in defining p53 functional status.Methods-Frozen tumour tissue from 94 patients with colorectal cancer was studied for p53 protein accumulation and gene mutations. Accumulation of p53 protein was detected by immunohistochemistry using PAb1801 and BP53-12-1 monoclonal antibodies. The findings were then compared with SSCP analysis of exons 5 to 8 of the p53 gene. All cases with a positive result by SSCP analysis were confirmed by sequencing.Results-Nuclear staining was observed in 51 (54.2%) cases. SSCP analysis of the DNA amplified by PCR revealed that the electrophoretic pattern had shifted in 30 cases; sequence analysis confirmed the occurrence of a mutation in 29 cases and of a polymorphism in one. In 27 cases both assays gave a positive result, and in 40 both were negative; therefore, concordance between PCR-SSCP and immunohistochemistry was seen in 72% of cases.Conclusion-The data indicate that positive immunostaining corresponds with the presence of a mutation in most, but not all, cases studied; other mechanisms could be responsible for stabilisation and accumulation of p53 protein in the nucleus. Nonsense mutations which do not confer stability on the protein will not be detected by immunohistochemistry and false negative results can also occur with SSCP analysis.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":"49 2","pages":"M85-90"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.2.m85","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26017606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Detection of clonal immunoglobulin gene rearrangements in the peripheral blood progenitor cells of patients with multiple myeloma: the potential role of purging with CD34 positive selection. 多发性骨髓瘤患者外周血祖细胞中克隆性免疫球蛋白基因重排的检测:CD34阳性选择清除的潜在作用

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m112

R G Owen, A P Haynes, P A Evans, R J Johnson, A C Rawstron, G McQuaker, G M Smith, M C Galvin, D L Barnard, N H Russell, J A Child, G J Morgan

{"title":"Detection of clonal immunoglobulin gene rearrangements in the peripheral blood progenitor cells of patients with multiple myeloma: the potential role of purging with CD34 positive selection.","authors":"R G Owen, A P Haynes, P A Evans, R J Johnson, A C Rawstron, G McQuaker, G M Smith, M C Galvin, D L Barnard, N H Russell, J A Child, G J Morgan","doi":"10.1136/mp.49.2.m112","DOIUrl":"https://doi.org/10.1136/mp.49.2.m112","url":null,"abstract":"Aims-To determine the extent of clonal cell contamination of peripheral blood progenitor cell (PBPC) collections in patients with multiple myeloma (MM) and to assess the purging efficacy of CD34 positive selection.Methods-PBPC collections from 29 patients with MM were analysed for the presence of clonal immunoglobulin heavy chain (IgH) gene rearrangements with a fluorescence based PCR technique. In addition, the PBPC from eight of the 29 patients were \"purged\" by selection of CD34 positive haematopoietic progenitors with an avidin-biotin immunoabsorption column (Ceprate). In each case the unmanipulated PBPC, CD34 positive and waste fractions were all assessed for the presence of clonal IgH rearrangements.Results-Clonal IgH rearrangements (identical with those demonstrated in diagnostic bone marrow samples) were demonstrated in 10 (35%) of 29 cases and seemed to be confined to those with significant residual bone marrow disease. Clonal rearrangements were evident in the PBPC of two of the eight patients who underwent CD34 selection; in both instances a \"clonal purge\" was seen as it was not possible to demonstrate the clonal rearrangement in the CD34 positive fraction. In four of the six remaining cases the normal polyclonal fingerprint could not be demonstrated in the CD34 positive fraction, which is consistent with a significant reduction in contaminating B cells.Conclusions-Clonal cells contaminate PBPC collections in a significant proportion of patients with MM and may be eliminated by CD34 positive selection.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":"49 2","pages":"M112-7"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.2.m112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26017601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Improved serodiagnosis of Lyme disease. 改善莱姆病的血清诊断。

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m80

M M Davidson, S M Chisholm, A D Wiseman, A W Joss, D O Ho-Yen

{"title":"Improved serodiagnosis of Lyme disease.","authors":"M M Davidson, S M Chisholm, A D Wiseman, A W Joss, D O Ho-Yen","doi":"10.1136/mp.49.2.m80","DOIUrl":"https://doi.org/10.1136/mp.49.2.m80","url":null,"abstract":"Aims-To determine whether enzyme linked immunosorbent assay (ELISA) results for Borrelia burgdorferi require confirmation by immunoblotting and how immunoblotting may best be used in the diagnosis of Lyme disease.Methods-Over one year, all referrals for Lyme disease to a district general hospital with a large tick population in its catchment area were tested by ELISA. Positive, low positive and negative serum samples were subjected to immunoblotting and the reactive bands analysed.Results-In total, 633 samples were received; 38 were ELISA positive and 97 low positive. More serum samples were from rural (n = 356) than from urban (n = 277) areas but a higher percentage of serum samples from urban areas were ELISA positive. The ELISA results were confirmed by immunoblotting in 15/38 positive samples but in only four of 37 with a low positive titre. An IgM positive blot required a 41 kDa band plus >/=1 specific band; for IgG a 41 kDa band plus >/=2 specific bands were necessary. Five serum samples were IgM positive with a 41 kDa plus one or more other specific bands. For IgG blots, the best discrimination was seen with the 21, 31, 46, and 92 kDa bands. Nonspecific, weakly reacting bands at 55, 60 and 67 kDa were frequently seen. Infection was confirmed in four of six patients with arthritis, but in only one of 10 patients with erythema chronicum migrans.Conclusions-ELISA alone is insufficient for diagnosis. All positive and low positive or negative serum samples with a good clinical history should be examined by immunoblotting. A higher percentage of modified ELISA positive than low positive results were confirmed. There are significant differences between European and American immunoblotting patterns. Local results show similarity to American results, highlighting the need for a local Borrelia isolate.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":"49 2","pages":"M80-4"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.2.m80","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26017605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Nomenclature for proteins: is calprotectin a proper name for the elusive myelomonocytic protein? 蛋白质的命名法:钙保护蛋白是难以捉摸的髓单核细胞蛋白的恰当名称吗?

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m74

M K Fagerhol

引用次数: 39

Relation between deletion of chromosome 1p36 and DNA ploidy in breast carcinoma: an interphase cytogenetic study. 乳腺癌1p36染色体缺失与DNA倍体的关系:间期细胞遗传学研究。

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.m98

F Farabegoli, N Baldini, D Santini, C Ceccarelli, M Taffurelli, D Treré, M Derenzini

{"title":"Relation between deletion of chromosome 1p36 and DNA ploidy in breast carcinoma: an interphase cytogenetic study.","authors":"F Farabegoli, N Baldini, D Santini, C Ceccarelli, M Taffurelli, D Treré, M Derenzini","doi":"10.1136/mp.49.2.m98","DOIUrl":"https://doi.org/10.1136/mp.49.2.m98","url":null,"abstract":"Aims-To investigate whether deletion of the 1p36 region of chromosome 1 is independent of DNA ploidy in breast cancer cells.Methods-Preparations of nuclei from 64 fresh primary breast tumours were studied using dual target fluorescence in situ hybridisation (FISH) combining probes specific for the 1q12 (pUC 1.77) and 1p36 (1p-79) regions of chromosome 1. Signals were counted in 100-300 nuclei and the percentage of cells showing fewer p1-79 than pUC 1.77 signals was measured in each sample. DNA ploidy was investigated by cytofluorimetry in 55 tumour samples.Results-Chromosome 1 aberrations were detected in 56 samples. There were fewer p1-79 than pUC 1.77 signals in 53 samples. The 1p36 region was deleted in 11 samples in which a single p1-79 signal was detected; seven of these samples were diploid. Abnormalities were found in 17/24 diploid and 30/31 aneuploid tumours.Conclusions-Chromosome 1 aberrations, including deletion of the 1p36 region, were observed in diploid breast tumours. Deletion of the 1p36 region may be an early event in tumorigenesis. Given the frequency and importance of chromosome 1 aberrations in the biological behaviour of breast tumours, FISH, used in conjunction with cytofluorimetry, may be helpful for determining prognosis in patients with diploid tumours.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":"49 2","pages":"M98-M103"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.2.m98","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26017609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Immune Reactions 免疫反应

Clinical molecular pathology Pub Date : 1996-04-01 DOI: 10.1136/mp.49.2.M124-b

I. Todd, C. Davenport

引用次数: 0

Accumulation of p53 protein in inverted transitional cell papilloma of the urinary bladder. 膀胱内翻性移行细胞乳头状瘤中p53蛋白的积累。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m43

K W Chan, K Y Lam, G Srivastava

{"title":"Accumulation of p53 protein in inverted transitional cell papilloma of the urinary bladder.","authors":"K W Chan, K Y Lam, G Srivastava","doi":"10.1136/mp.49.1.m43","DOIUrl":"https://doi.org/10.1136/mp.49.1.m43","url":null,"abstract":"Aims-To study the possible accumulation of p53 protein in inverted papilloma of the urinary bladder.Methods-Formalin fixed, paraffin wax embedded sections from 14 cases of inverted papilloma of the urinary bladder were studied retrospectively. Accumulation of p53 was detected by immunohistochemistry using a mouse monoclonal antibody directed against p53. p53 protein reactivity was scored as follows: 0 = 10%; 1 = 10% to <30%; 2 = 30% to <50%; and 3 = >50% of cells p53 positive.Results-The 14 sections were scored as follows: 3 in four cases; 2 in four cases; 1 in one case; and 0 in five cases. Overall, nine (64%) of the 14 cases were positive for p53 protein.Conclusions-The accumulation of p53 protein in inverted papilloma of the urinary bladder suggests that p53 may have has an important role in the neoplastic process of this tumour. However, the benign nature of inverted papillomas suggests that p53 protein accumulation is not related to tumour invasiveness and metastasis. p53 reactivity cannot be used as a marker of malignancy for urothelial neoplasia. Further studies are required to determine the role of p53 protein in the oncogenesis of urothelial neoplasms.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":"49 1","pages":"M43-5"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.1.m43","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26021480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Molecular and biochemical aspects of interphase nucleolar organiser regions. 间期核仁组织者区的分子和生化方面。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m8

J Crocker

引用次数: 6

Establishment and characterisation of two cell lines derived from a primary adenocarcinoma of the duodenum. 原发性十二指肠腺癌两株细胞系的建立和特性分析。

Clinical molecular pathology Pub Date : 1996-02-01 DOI: 10.1136/mp.49.1.m33

M Golding, G W Stamp, T Oates, E N Lalani

引用次数: 2