Expression of type III collagen mRNA in renal biopsy specimens of patients with idiopathic membranous glomerulonephritis.

Clinical molecular pathology Pub Date : 1996-02-01 DOI:10.1136/mp.49.1.m40

M S Razzaque, T Taguchi

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引用次数: 11

Abstract

Aim-To investigate the distribution of type III collagen in membranous glomerulonephritis (MGN); to identify the cells responsible for the synthesis of alpha1 (III) mRNA.method-The distribution of type III collagen was studied by immunohistochemistry in 10 renal biopsy specimens, histologically diagnosed as MGN, and five control renal tissue samples obtained at surgery. Synthesis of alpha1 (III) mRNA was detected by non-radioactive in situ hybridisation.Results-On immunohistochemistry, type III collagen was not observed in the control glomeruli, but was present focally in the glomeruli in samples from patients with MGN. No specific hybridisation signal for alpha1 (III) mRNA was found in the control glomeruli on non-radioactive in situ hybridisation. By contrast, positive signals for alpha1 (III) chain mRNA were detected in glomerular epithelial cells and mesangial cells in MGN tissue samples.Conclusion-These data suggest that additional synthesis of type III collagen by intraglomerular cells contributes to the changes in the glomerular basement membrane characteristic of MGN.

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特发性膜性肾小球肾炎患者肾活检标本中III型胶原mRNA的表达。

目的:探讨III型胶原蛋白在膜性肾小球肾炎(MGN)中的分布;鉴定负责合成alpha1 (III) mRNA的细胞。方法:采用免疫组化方法对10例经组织学诊断为MGN的肾活检标本和5例手术后的对照肾组织标本进行III型胶原的分布研究。用非放射性原位杂交法检测α 1 (III) mRNA的合成。结果免疫组化结果显示，在对照组肾小球中未观察到III型胶原蛋白，但在MGN患者的肾小球中存在局部胶原蛋白。对照肾小球非放射性原位杂交未发现α 1 (III) mRNA特异性杂交信号。相比之下，在MGN组织样本的肾小球上皮细胞和系膜细胞中检测到α 1 (III)链mRNA的阳性信号。结论:这些数据表明，肾小球内细胞额外合成III型胶原有助于肾小球基底膜特征的改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical molecular pathology

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