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Basic and applied histochemistry最新文献

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Ultrahistochemical demonstration of guanylate cyclase activity in the rat hippocampus. 大鼠海马鸟苷酸环化酶活性的超微组织化学证明。
Basic and applied histochemistry Pub Date : 1989-01-01
G Poeggel, H Luppa
{"title":"Ultrahistochemical demonstration of guanylate cyclase activity in the rat hippocampus.","authors":"G Poeggel,&nbsp;H Luppa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Guanylate cyclase activity was demonstrated in the rat hippocampus at the electron microscopy level by a cerium precipitation technique. Enzymatic activity is localized in the granular cells of the dentate gyrus and in other, medium-sized neurons. The reaction product was found on the cytoplasmic membrane, in the cytoplasm, on the surface of endoplasmic reticulum and in postsynaptic densities. Glial cell processes and small dendritic processes were also stained. Besides the neurons, glial cells contained strong enzymatic activity, especially the perivascular glia and protoplasmatic glial cells neighbouring to neurons. Only little activity was detected in pyramidal cells and no activity was found in the endothelium cells of capillaries.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 2","pages":"121-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13711666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In praise of dyestuff histochemistry. 赞扬染料组织化学。
Basic and applied histochemistry Pub Date : 1989-01-01
J E Scott
{"title":"In praise of dyestuff histochemistry.","authors":"J E Scott","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The merits of low molecular weight reagents (mini-stains) for localising tissue components, are compared with those of high molecular weight (macro-stains), with respect to stoichiometry, ease of tissue penetration, and particularly specificity. Macro-stains, which are based mainly on antibodies, are likely to be more specific, but this will not always be the case. The concept of specificity in the design of mini-stains is examined, and its application to the delivery of e.g. Cupromeronic blue to particular substrates in the \"critical electrolyte concentration\" system, is discussed. The much greater resolving power of mini-stains in electron histochemistry has permitted the elucidation of four specific binding sites for proteoglycans along the collagen fibrils, validating the 'one proteoglycan: one binding site' hypothesis.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13871370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunohistological detection of H-2 antigens in vivo induced by IFN-beta in mouse brain tissues. ifn - β诱导小鼠脑组织H-2抗原的免疫组织学检测。
Basic and applied histochemistry Pub Date : 1989-01-01
Y Fueta, T Kita, T Mita
{"title":"Immunohistological detection of H-2 antigens in vivo induced by IFN-beta in mouse brain tissues.","authors":"Y Fueta,&nbsp;T Kita,&nbsp;T Mita","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We describe the immunohistological detection of in vivo interferon-beta (IFN-beta)-induced H-2 antigens in mouse brain tissues. Two injections of recombinant mouse interferon-beta (rIFN-beta) were given into the lateral ventricles. Following perfusion with periodate-lysine-paraformaldehyde (PLP) solution, microsliced brain sections were incubated with anti H-2 monoclonal antibody and these subjected to a streptavidin biotin immunohistochemistry technique. Expression of H-2 antigens was demonstrated on neurons, neuroglias and vascular endothelial cells in the hippocampus, cerebral cortex, corpus callosum and other regions of the brain as well as on ependymocytes in the choroid plexus. H-2 antigens were also diffusely distributed in the cytoplasm and axons.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 2","pages":"131-7"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13898316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Changes in the organization of the extracellular matrix in ovarian follicles during the preovulatory phase and atresia. An immunofluorescence study. 卵巢卵泡细胞外基质组织在排卵期和闭锁期间的变化。免疫荧光研究。
Basic and applied histochemistry Pub Date : 1989-01-01
M Bortolussi, R Zanchetta, R Doliana, I Castellani, G M Bressan, A Lauria
{"title":"Changes in the organization of the extracellular matrix in ovarian follicles during the preovulatory phase and atresia. An immunofluorescence study.","authors":"M Bortolussi,&nbsp;R Zanchetta,&nbsp;R Doliana,&nbsp;I Castellani,&nbsp;G M Bressan,&nbsp;A Lauria","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The distribution of laminin, type IV collagen and fibronectin was studied by immunofluorescence in rat, pig and cow ovarian follicles. The results obtained in the three species investigated were similar. In all the follicles, laminin and type IV collagen were identically localized in the basal lamina (BL) separating the granulosa and the theca layers. In addition, these two proteins were also distributed in the wall of blood vessels of the thecae and ovarian stroma. The staining showed that the BL of primordial and growing follicles was regular and continuous, but underwent striking modifications during ovulation and atresia. In fact, in preovulatory follicles the BL appeared thinner and discontinuous, whereas it was much thickened and ruptured in atretic follicles. Fibronectin was localized mainly in inner granulosa cells of small and medium-sized growing follicles, and as a broad and irregular layer around the cavity of the degenerated follicles. The results show that each stage of follicular growth and involution is associated with a precise and peculiar pattern of distribution of laminin, type IV collagen and fibronectin. The possibility that these proteins play a role in the local control of ovarian follicular dynamics is advanced.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 1","pages":"31-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13799467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The harderian gland of the frog, Rana esculenta, during the annual cycle: histology, histochemistry and ultrastructure. 蛙蛙(Rana esculenta)年循环中的硬腺:组织学、组织化学和超微结构。
Basic and applied histochemistry Pub Date : 1989-01-01
L Di Matteo, S Minucci, G Chieffi Baccari, C Pellicciari, M d'Istria, G Chieffi
{"title":"The harderian gland of the frog, Rana esculenta, during the annual cycle: histology, histochemistry and ultrastructure.","authors":"L Di Matteo,&nbsp;S Minucci,&nbsp;G Chieffi Baccari,&nbsp;C Pellicciari,&nbsp;M d'Istria,&nbsp;G Chieffi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Harderian gland in Rana esculenta has been studied during the annual cycle at the histological, histochemical and ultrastructural levels. The Harderian gland has an acinar structure and is the only orbital gland in anuran amphibia. It develops at the medial corner of the orbit from the conjunctival epithelium at the premetamorphic stage. In the adult the glandular secretion reaches a maximum during the months of July and August, drops in September and resumes slowly from October onwards. The secretion is seromucoid and the secretory granules are released into the acinar lumen, mainly by exocytosis. Porphyrins were not detected. No sexual dimorphism was observed in the glandular cells. The resumption of secretory activity in October and the enhancement of secretion in May are marked by the appearance of \"blue nuclei\" (Mallory stain) in a relatively high percentage of glandular cells. This unusual blue colour, using the Mallory stain (by which nuclei stain red), disappears after digestion of paraffin sections with RNAase, but not with DNAase and trypsin. The blue staining may, therefore, indicate an increased amount of nuclear RNA. The Harderian gland in the frog most probably serves to lubricate and moisten the eye in the absence of the lacrimal gland. However, the gland may also represent an immunoactive organ owing to the presence of numerous mast cells and plasma cells in the interacinar spaces.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 2","pages":"93-112"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13927041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of SV40-transformed human cells by immunofluorescence and fluorescent in situ hybridization techniques. 免疫荧光和荧光原位杂交技术表征sv40转化的人细胞。
Basic and applied histochemistry Pub Date : 1989-01-01
A Prelle, M Moggio, G Fagiolari, G Scarlato, A Miranda, E Bonilla
{"title":"Characterization of SV40-transformed human cells by immunofluorescence and fluorescent in situ hybridization techniques.","authors":"A Prelle,&nbsp;M Moggio,&nbsp;G Fagiolari,&nbsp;G Scarlato,&nbsp;A Miranda,&nbsp;E Bonilla","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have performed immunofluorescent and fluorescent in situ hybridization studies in order to better clarify the integration of SV40 DNA in human fibroblast cell lines. Most of the cells were T-antigen positive by immunocytochemical studies, while in all the cells we detected the integrated viral DNA by in situ hybridization. Both techniques are easy and useful to perform but the molecular genetic method gives a more specific signal with the possibility of localizing molecular hybrids in the nucleus and in the cytoplasm of the transformed cells.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 3","pages":"179-83"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13698351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lectin histochemistry of secretory cell glycoconjugates in the nasal mucosa of Podarcis sicula campestris De Betta (Reptilia, Lacertidae). 斑点足鼠鼻黏膜分泌细胞糖结合物的凝集素组织化学研究。
Basic and applied histochemistry Pub Date : 1989-01-01
D Ferri, G E Liquori
{"title":"Lectin histochemistry of secretory cell glycoconjugates in the nasal mucosa of Podarcis sicula campestris De Betta (Reptilia, Lacertidae).","authors":"D Ferri,&nbsp;G E Liquori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Histochemical study by traditional staining methods (AB, PAS, HID) and by the use of five peroxidase-labelled lectins (ConA, WGL, WPL, SBL, PNL) were carried out to characterize glycoconjugates in the secretory cells of the nasal mucosa of the Lacertid lizard Podarcis sicula campestris De Betta. The mucus covering the nasal epithelium is produced by the supporting cells and the Bowman glands in the olfactory area, and by typical goblet cells and, probably, a second type of secretory cell, in the non-sensory area. Neutral glycoconjugates containing N-acetyl-D-glucosamine and terminal N-acetyl-D-galactosamine, D-mannose and D-glucose residues were present in the secretory product of the Bowman glands. L-fucose and D-galactose were absent. In the supporting cells the secretory product consisted mainly of sulfated glycoproteins containing D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-mannose, D-glucose, but not L-fucose. Glycoconjugates containing terminal sialic acid and penultimate D-galactose were present in typical goblet cells as was N-acetyl-D-glucosamine.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 3","pages":"197-207"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13956808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochemical, histochemical and immunohistochemical study of glycosaminoglycans in human meningiomas. 人脑膜瘤中糖胺聚糖的生化、组织化学和免疫组织化学研究。
Basic and applied histochemistry Pub Date : 1989-01-01
A Bertolotto, M T Giordana, L Orsi, R Orsi, D Schiffer
{"title":"Biochemical, histochemical and immunohistochemical study of glycosaminoglycans in human meningiomas.","authors":"A Bertolotto,&nbsp;M T Giordana,&nbsp;L Orsi,&nbsp;R Orsi,&nbsp;D Schiffer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The localization and quantitation of glycosaminoglycans classes (GAGs) were studied in human meningiomas. Meningiomas presented high amounts of these compounds and electrophoretic separation revealed that they were 90% sulphated. The Alcian method and a polyclonal antiserum against chondroitin sulphate were used to localize the different GAGs in tissue sections. Quantitative and qualitative differences and different tissue distributions of GAGs were observed among transitional, syncytial and fibroblastic meningiomas. Syncytial meningiomas presented the lowest amount of GAGs and the immuno- and histochemical studies showed that they were located only in vessels and connectival trabeculae. Transitional meningiomas contained the highest concentration of GAGs; the percentage of the different GAG classes was similar to that observed in the syncytial oncotype indicating a quantitative but not qualitative difference between the two oncotypes. The high amount of GAGs in transitional meningiomas was attribute to the whorls, the structures stained by the histochemical and immunohistochemical techniques. The tumoral parenchyma of these two oncotypes was negative. On the contrary, fibroblastic meningiomas showed a fine meshwork among tumoral cells containing chondroitin sulphate and heparan sulphate. Biochemical data were consistent with the histochemical and immunohistochemical findings revealing a high percentage of chondroitin sulphate and heparan sulphate in fibroblastic meningiomas. This study suggests that the three meningioma types have different abilities to produce extracellular matrix components.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 3","pages":"239-49"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13956810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proceedings of the XXIII National Congress of the Italian Society of Histochemistry. Pavia, 18-20 September 1989. Abstracts. 意大利组织化学学会第二十三届全国大会论文集。帕维亚，1989年9月18日至20日。摘要。
Basic and applied histochemistry Pub Date : 1989-01-01
{"title":"Proceedings of the XXIII National Congress of the Italian Society of Histochemistry. Pavia, 18-20 September 1989. Abstracts.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 Suppl ","pages":"5-119"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13761690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Basal cells of the frog's taste organ: fluorescence histochemistry with the serotonin analogue 5,7-dihydroxytryptamine in supravital conditions. 青蛙味觉器官的基底细胞:在生存条件下与血清素类似物5,7-二羟色胺的荧光组织化学。
Basic and applied histochemistry Pub Date : 1989-01-01
A Sbarbati, C Zancanaro, F Franceschini, F Osculati
{"title":"Basal cells of the frog's taste organ: fluorescence histochemistry with the serotonin analogue 5,7-dihydroxytryptamine in supravital conditions.","authors":"A Sbarbati,&nbsp;C Zancanaro,&nbsp;F Franceschini,&nbsp;F Osculati","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We utilized the fluorescent serotonin analogue 5,7-dihydroxytryptamine (5,7DHT) to visualize basal cells in the frog's taste organ in supravital conditions. In whole mounts of lingual mucosa, specifical and detailed morphological visualization of fluorescent basal cells was obtained in the peripheral and central region of the intact taste organ; similar results were obtained after mechanical dissociation. Preincubation with serotonin prevented any fluorescence in basal cells. Electron microscopy showed good preservation of the ultrastructural morphology of the taste disk after exposure to 5,7DHT. The advantages of the current method as compared with conventional ones are discussed. This simple, reliable procedure will be useful to further define the biology of neuroendocrine cells in taste as well as in other organs.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":"33 4","pages":"289-97"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13770853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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