ifn - β诱导小鼠脑组织H-2抗原的免疫组织学检测。

Basic and applied histochemistry Pub Date : 1989-01-01
Y Fueta, T Kita, T Mita
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引用次数: 0

摘要

我们描述了体内干扰素- β (ifn - β)诱导的小鼠脑组织中H-2抗原的免疫组织学检测。在侧脑室注射2次重组小鼠干扰素- β (rifn - β)。在高碘酸-赖氨酸-多聚甲醛(PLP)溶液灌注后,用抗H-2单克隆抗体孵育脑切片,并进行链霉亲和素生物素免疫组织化学技术。H-2抗原在海马、大脑皮层、胼胝体等脑区神经元、神经胶质细胞和血管内皮细胞以及脉络膜丛室管膜细胞上均有表达。H-2抗原也广泛分布于细胞质和轴突。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistological detection of H-2 antigens in vivo induced by IFN-beta in mouse brain tissues.

We describe the immunohistological detection of in vivo interferon-beta (IFN-beta)-induced H-2 antigens in mouse brain tissues. Two injections of recombinant mouse interferon-beta (rIFN-beta) were given into the lateral ventricles. Following perfusion with periodate-lysine-paraformaldehyde (PLP) solution, microsliced brain sections were incubated with anti H-2 monoclonal antibody and these subjected to a streptavidin biotin immunohistochemistry technique. Expression of H-2 antigens was demonstrated on neurons, neuroglias and vascular endothelial cells in the hippocampus, cerebral cortex, corpus callosum and other regions of the brain as well as on ependymocytes in the choroid plexus. H-2 antigens were also diffusely distributed in the cytoplasm and axons.

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