In praise of dyestuff histochemistry.

Basic and applied histochemistry Pub Date : 1989-01-01
J E Scott
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引用次数: 0

Abstract

The merits of low molecular weight reagents (mini-stains) for localising tissue components, are compared with those of high molecular weight (macro-stains), with respect to stoichiometry, ease of tissue penetration, and particularly specificity. Macro-stains, which are based mainly on antibodies, are likely to be more specific, but this will not always be the case. The concept of specificity in the design of mini-stains is examined, and its application to the delivery of e.g. Cupromeronic blue to particular substrates in the "critical electrolyte concentration" system, is discussed. The much greater resolving power of mini-stains in electron histochemistry has permitted the elucidation of four specific binding sites for proteoglycans along the collagen fibrils, validating the 'one proteoglycan: one binding site' hypothesis.

赞扬染料组织化学。
在化学计量学、组织渗透便捷性和特异性方面,比较了用于定位组织成分的低分子量试剂(小染色剂)与高分子量试剂(大染色剂)的优点。主要基于抗体的宏观染色可能更具有特异性,但情况并非总是如此。研究了微型污渍设计中的特异性概念,并讨论了其在“临界电解质浓度”系统中向特定底物递送例如铜丙二酸蓝的应用。在电子组织化学中,微型染色具有更大的分辨能力,可以阐明蛋白多糖沿着胶原原纤维的四个特定结合位点,从而验证了“一个蛋白多糖:一个结合位点”的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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