{"title":"Lectin histochemistry of secretory cell glycoconjugates in the nasal mucosa of Podarcis sicula campestris De Betta (Reptilia, Lacertidae).","authors":"D Ferri, G E Liquori","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Histochemical study by traditional staining methods (AB, PAS, HID) and by the use of five peroxidase-labelled lectins (ConA, WGL, WPL, SBL, PNL) were carried out to characterize glycoconjugates in the secretory cells of the nasal mucosa of the Lacertid lizard Podarcis sicula campestris De Betta. The mucus covering the nasal epithelium is produced by the supporting cells and the Bowman glands in the olfactory area, and by typical goblet cells and, probably, a second type of secretory cell, in the non-sensory area. Neutral glycoconjugates containing N-acetyl-D-glucosamine and terminal N-acetyl-D-galactosamine, D-mannose and D-glucose residues were present in the secretory product of the Bowman glands. L-fucose and D-galactose were absent. In the supporting cells the secretory product consisted mainly of sulfated glycoproteins containing D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-mannose, D-glucose, but not L-fucose. Glycoconjugates containing terminal sialic acid and penultimate D-galactose were present in typical goblet cells as was N-acetyl-D-glucosamine.</p>","PeriodicalId":8726,"journal":{"name":"Basic and applied histochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basic and applied histochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Histochemical study by traditional staining methods (AB, PAS, HID) and by the use of five peroxidase-labelled lectins (ConA, WGL, WPL, SBL, PNL) were carried out to characterize glycoconjugates in the secretory cells of the nasal mucosa of the Lacertid lizard Podarcis sicula campestris De Betta. The mucus covering the nasal epithelium is produced by the supporting cells and the Bowman glands in the olfactory area, and by typical goblet cells and, probably, a second type of secretory cell, in the non-sensory area. Neutral glycoconjugates containing N-acetyl-D-glucosamine and terminal N-acetyl-D-galactosamine, D-mannose and D-glucose residues were present in the secretory product of the Bowman glands. L-fucose and D-galactose were absent. In the supporting cells the secretory product consisted mainly of sulfated glycoproteins containing D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-mannose, D-glucose, but not L-fucose. Glycoconjugates containing terminal sialic acid and penultimate D-galactose were present in typical goblet cells as was N-acetyl-D-glucosamine.
采用传统染色法(AB、PAS、HID)和五种过氧化物酶标记凝集素(ConA、WGL、WPL、SBL、PNL)对斑点蜥鼻黏膜分泌细胞糖缀合物进行了组织化学研究。覆盖鼻上皮的黏液是由嗅觉区的支持细胞和鲍曼腺,以及典型的杯状细胞和非感觉区的第二种分泌细胞产生的。鲍曼腺分泌产物中含有n -乙酰- d -葡萄糖胺和末端n -乙酰- d -半乳糖胺、d -甘露糖和d -葡萄糖残基的中性糖缀合物。L-灶和d -半乳糖缺失。在支持细胞中,分泌产物主要是含有d -半乳糖、n -乙酰- d -半乳糖、n -乙酰- d -葡萄糖胺、d -甘露糖和d -葡萄糖的硫酸糖蛋白,但不含L-聚焦。典型杯状细胞中含有末端唾液酸和倒数第二的d -半乳糖的糖缀合物和n -乙酰- d -氨基葡萄糖。