{"title":"Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments","authors":"Lovely Aktar, M. Saha","doi":"10.3329/bjb.v52i4.70580","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70580","url":null,"abstract":"The present study aims to identify prospective lipase-producing bacteria and optimize different culture parameters to produce maximum lipase. A total of 37 lipase-positive bacteria were isolated from lipid-rich environments. Among them, strains S1N-2 and S10P-1 were found to have potential and were molecularly identified as Stenotrophomonas species ICMM10 and Pseudomonas aeruginosa BA7823, respectively. For maximum lipase production, the optimum pH was 7 and the temperature was 37ºC. Using these conditions, the isolates were grown on media with additional carbon and nitrogen sources along with different inoculum concentrations. The maximum amount of lipase produced by Stenotrophomonas sp. ICMM10 was 136.47 ± 1.63 U/ml, which is 5-fold higher than before. Pseudomonas aeruginosa BA7823 produced a maximum lipase of 96.33 ± 0.21 U/ml, which was about 9-fold higher than before. These results would provide useful information about lipase production by indigenous isolates, which may be employed as a potential bacterial lipase source of biotechnological interest. Bangladesh J. Bot. 52(4): 979-988, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"73 10","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139132296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Axillary shoot proliferation and regeneration of red rose [pisiformis (christ.) D. Sosn. An under thread extinction species endemic to Turkey","authors":"Onur Özel, Hatice Demiray, Volkan Eroğlu, Cihan Ecer","doi":"10.3329/bjb.v52i4.70576","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70576","url":null,"abstract":"In vitro propagation of red rose [Rosa pisiformis (Christ.) D. Sosn.] has great importance in rapid proliferation of species with medicinal features and in culture of healthy plants free from deseases. After a succesfully pre-sterilization procedure, experiments were maintained by two-phase: multiplication phase and rooting of microshoots phase. Micropropagation of Rose was improved, using its nodal segments under different combination of BAP (0, 1.0, 2.0, 0.6 mg/l), NAA (0, 0.1, 0.2, 0.01 mg/l) on Driver and Kuniyuki (DKW) and MS medium in multiplication phase and using distinct compositions of IBA (0, 1.0, 3.0, 5.0 mg/l) and DKW medium in rooting phase. The maximum number of shoots/per plant (22.6) with the highest number of leaves (222.6) were obtained in DKW medium supplemented with 0.6 mgl-1 BAP and 0.01 mg/l NAA. The leaf explants were also used to occur callus in MS with different combinations of BAP and NAA. Good callus formation was obtained with 1:2 ratio of BAP:NAA combination in MS medium. Furthermore, the highest root induction (100%) was achieved in DKW medium consisting of 1 mg/l IBA. The rooted plantlets were transferred to the field after acclimatized in greenfield conditions. The present investigation presents an in vitro protocol for R. pisiformis. Bangladesh J. Bot. 52(4): 949-958, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"111 36","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139133334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Dash, Nowshin Anjum, Prasenjit Roy, Syeda Sharmeen Sultana
{"title":"Karyotype profile of holarrhena antidysenterica (Roxb. Ex fleming) wall","authors":"C. Dash, Nowshin Anjum, Prasenjit Roy, Syeda Sharmeen Sultana","doi":"10.3329/bjb.v52i4.70609","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70609","url":null,"abstract":"The karyomorphological features of Holarrhena antidysenterica (Roxb. ex Fleming) Wall. through the application of differential staining techniques (using orcein, CMA, and DAPI) were studied. After applying orcein staining, the interphase nuclei of this species exhibited a ‘simple chromocenter type’ with numerous small heterochromatin blocks and the prophase chromosomes were ‘interstitial type’. The karyotype analysis revealed that this species possesses diploid chromosome number of 2n = 22, with all metacentric chromosomes. The total length of the chromosome complement in the diploid set was 29.91 μm. A total of four CMA-positive bands were observed in this species whereas seven bands were found after DAPI-staining. Heteromorphicity was observed in the DAPI stained metaphase stage indicating the probable occurrences of chromosomal aberrations such as deletion and inversion. The karyotype profile of H. antidysenterica obtained from orcein and fluorescent banding was determined for the first time in Bangladesh. Bangladesh J. Bot. 52(4): 1085-1089, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"101 19","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Sangwan, Rakesh Sangwan, Vinod Malik, Manjeet Singh
{"title":"Evaluation of antibiotics and bioagents for the management of black rot disease of cabbage and their effect on extracellular polysaccharide secretion","authors":"P. Sangwan, Rakesh Sangwan, Vinod Malik, Manjeet Singh","doi":"10.3329/bjb.v52i4.70578","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70578","url":null,"abstract":"Black rot disease caused by bacterium Xanthomonas campestris pv. campestris has been identified as major cause for low cabbage yields. The present study was conducted to evaluate the efficacy of various antibiotics and bioagents for controlling black rot disease and also their effect on extracellular polysaccharide secretion (EPS) of Xanthomonas campestris pv. campestris. Under in vitro conditions, bacterial proliferation and EPS production were examined on yeast peptone agar medium containing sucrose or trehalose with or without antibiotics. Number of Xanthomonas campestris pv. campestris on yeast peptone agar medium with Validamycin-A was less as compared to control. Extracellular polysaccharide secretion (EPS) was also inhibited on yeast peptone agar medium with antibiotic Validamycin-A. In vivo, disease intensity was recorded minimum in seed treatment + root dipping + foliar spray with Validamycin-A @ 500 µg/ml followed by seed treatment with Validamycin-A @ 500 µg/ml in comparison to control. Similarly, 45.61% higher cabbage yield was recorded in seed treatment + root dipping + foliar spray with Validamycin-A @ 500 as compared to untreated control. However, the antibiotic Validamycin-A which was found effective can be recommended to manage the disease. Bangladesh J. Bot. 52(4): 965-970, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"71 5","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Fu, Hong Zhang, Tieqi Xia, Xue Yang, Dingwang, Wu-ling Chen
{"title":"Potential of compound bacterial agent in the biocontrol of muskmelon pathogens","authors":"R. Fu, Hong Zhang, Tieqi Xia, Xue Yang, Dingwang, Wu-ling Chen","doi":"10.3329/bjb.v52i4.70603","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70603","url":null,"abstract":"The bacterial strain TG6 was screened from the soil of a muskmelon greenhouse, which could effectively inhibit the growth of Sphaerotheca fuliginea, Fusarium oxysporum, Phytophthora melonis and Pseudoperonospora cubensis of muskmelons. TG6 was identified as Bacillus subtilis through morphological, physiological, biochemical and molecular studies. Taking TG6 as the original strain, TG67 and TG69 were obtained by He-Ne laser mutagenesis. The microbial compound antagonist strain TG67 and TG69 was prepared in the ratio of 1:1 and applied to the biological control of greenhouse melon. Muskmelon field experiments showed that the bactericide could effectively control the occurrence of various diseases of muskmelon in greenhouse. Bangladesh J. Bot. 52(4): 1047-1053, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"101 11","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139133853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of seedling dipping and foliar application of nano dap on growth, yield and economics of fine rice","authors":"Meenakshi Attri, Neetu Sharma, Swati Mehta, Joysamuel Mecarty","doi":"10.3329/bjb.v52i4.70589","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70589","url":null,"abstract":"A field experiment was conducted to assess the effect of seedling dipping and foliar application of phosphorus on growth, yield and economics of fine rice. The experimental results revealed that 75% recommended nitrogen, phosphorus and 100% recommended potassium + seedling dipping with nano di-ammonium phosphate @ 5 ml/litre + foliar spray with nano di-ammonium phosphate @ 4 ml/litre application recorded significantly higher growth, yield and yield attributes, which was statistically at par with, 50% recommended nitrogen, phosphorus +100% recommended potassium + seedling treatment with nano di-ammonium phosphate @ 5 ml/litre+ 2 foliar spray with nano di-ammonium phosphate each @ 4 ml/litre and @ 2 ml/litre and 100% recommended nitrogen, phosphorus and potassium. However, 50% recommended nitrogen, phosphorus +100% recommended potassium + seedling treatment with nano di-ammonium phosphate @ 5 ml/litre+ 2 foliar spray with nano nano di-ammonium phosphate each @ 4 ml/litre recorded significantly highest agro physiological efficiency. However, in terms of economics 75% recommended nitrogen, phosphorus and 100% recommended potassium + seedling dipping with nano di-ammonium phosphate @ 5 ml/litre + foliar spray with nano nano di-ammonium phosphate @ 4 ml/litre recorded highest net returns and benefit cost ratio. Bangladesh J. Bot. 52(4): 1025-1031, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"87 19","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139132217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Susmita Mondal, F. T. Johora, Goutom Roy, Jamilur Rahman
{"title":"Genotype and environment interactions of yield contributing characters of field mustard (Brassica rapa L.)","authors":"Susmita Mondal, F. T. Johora, Goutom Roy, Jamilur Rahman","doi":"10.3329/bjb.v52i4.70604","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70604","url":null,"abstract":"Genotype-environment interaction is the norm of reciprocal action that determines the relationship between gene and environmental factors. Gene and environment interaction takes place when different environments affect the genotypes and vice versa. The investigation portrays the interaction between genes and environments of yield contributing characteristics of eleven genotypes of field mustard. Three seeding-time environments, viz., early, late, and very late of eleven mustard genotypes were laid out with three replications in a randomized complete block design. Among the three environments, which one was favorable and which was not was determined by their significant differences through a combined analysis of variance. The IPCA 1 (first interaction principal component axis) score of genotypes in the AMMI (additive main effects and multiplicative interaction analysis) of G×E interactions was estimated as an indication of the ability to respond to the environments and the performance with changing environments of the genotypes. Considering all the scores, Sonali Sharisha (SS-75), and BINA Sharisha-10 were found to be highly stable genotypes, while among the three environments, the environment-1 (early sowing) was found the best sowing time for raising and harvesting a good mustard crop. Bangladesh J. Bot. 52(4): 1055-1065, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"69 8","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139132401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization and diversity analysis of promising tgms lines and high-yieding varieties In rice by using ssr markers","authors":"V. Karpagam, R. Kalaiyarasi","doi":"10.3329/bjb.v52i4.70610","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70610","url":null,"abstract":"The present investigation was intended to assess the extent of genetic diversity present among the selected genotypes of Oryza sativa (including TGMS lines) using SSR markers. The SSR markers used for the study gave average Polymorphism Information Content (PIC) value of 0.551. The primer RM 9 followed by RM 536 has given maximum PIC value and the primer RM 29 gave the minimum. It was inferred that the primers RM 9 and RM 536 had been the better choices in this study. The lines, TS09 24 and TS09 26 were in the same cluster as they derived from the same parents. The TGMS lines TS 29 and TNAU 27S are in separate cluster from other parents indicating that these lines are highly diversified and their crosses can give improved cultivars. Bangladesh J. Bot. 52(4): 1067-1070, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"4 5","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in internal structure and nutritional quality with different types of maize kernels during Grain filling period","authors":"Qiang Guo, Juan Li, Jingjing Cui, Huadi She","doi":"10.3329/bjb.v52i4.70608","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70608","url":null,"abstract":"To understand the changes in grain internal structure and nutrients during maize filling, Jingkenuo 2000 (waxy corn), Chaosweet 2000 (sweet corn), and Zhengdan 958 (common corn) were used as test materials. This study observed the internal structure of the corn grain and measured the change in nutritional quality during the filling period. Throughout the determination stage, the starch granules did not fill the whole endosperm cell in Chaosweet 2000, and the cell wall was clearly visible. By contrast, the starch granules filled the whole endosperm cell in Jingkenuo 2000 and Zhengdan 958. The protein content in Chaosweet 2000 was significantly (P<0.05) higher than those in Jingkenuo 2000 and Zhengdan 958. With the extension of post-pollination time, the content of soluble sugar decreased significantly (P<0.05) in Chaosweet 2000, Jingkenuo 2000, and Zhengdan 958. Bangladesh J. Bot. 52(4): 1077-1084, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"105 7","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139133776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects Of Organic Substance And Plant Growth Regulators On Yield Attributes And Quality Of Guava Cv. Lucknow 49","authors":"DM Dabhi, Mj Patel, SJ Macwan","doi":"10.3329/bjb.v52i4.70579","DOIUrl":"https://doi.org/10.3329/bjb.v52i4.70579","url":null,"abstract":"In the present study effects of organic substance and plant growth regulators on yield attributes and quality of guava cv. Lucknow 49 were conducted during 2021-2022. Results indicated that foliar application of novel organic liquid fertilizer (40 ml/l) gave higher yield parameters viz. number of flowers per branch, fruit set, weight per fruit, fruit volume, fruit diameter, fruit length, pulp weight per fruit, number of fruits per plant, fruit yield (kg/plant) during both the years and in pooled data. In quality parameters, higher chlorophyll content and pulp: seed ratio were recorded with novel organic liquid fertilizer (40 ml/l) during both the years and in pooled data. However, organic substance and plant growth regulators treatments showed non-significant effect on total seed weight per fruit in both the years as well as in pooled data. Bangladesh J. Bot. 52(4): 971-978, 2023 (December)","PeriodicalId":8703,"journal":{"name":"Bangladesh Journal of Botany","volume":"111 12","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139135288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}