holarrhena antidysenterica（Roxb.

Pub Date : 2023-12-31 DOI:10.3329/bjb.v52i4.70609

C. Dash, Nowshin Anjum, Prasenjit Roy, Syeda Sharmeen Sultana

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引用次数: 0

摘要

通过应用不同的染色技术（使用orcein、CMA和DAPI），研究了Holarrhena antidysenterica (Roxb. ex Fleming) Wall.的核形态学特征。在使用橙皮苷染色后，该物种的间期细胞核表现为 "简单染色中心型"，其中有许多小的异染色质块，而前期染色体则为 "间隙型"。核型分析表明，该物种的二倍体染色体数为 2n = 22，全部为偏心染色体。二倍体染色体互补体的总长度为 29.91 μm。该物种共观察到四条 CMA 阳性条带，而 DAPI 染色后则发现七条条带。在 DAPI 染色的移行期观察到异形，表明可能存在染色体畸变，如缺失和倒位。在孟加拉国，首次确定了通过橙皮苷和荧光带染色获得的 H. antidysenterica 的核型特征。孟加拉国 J. Bot.52(4):1085-1089, 2023 (December)

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Karyotype profile of holarrhena antidysenterica (Roxb. Ex fleming) wall

The karyomorphological features of Holarrhena antidysenterica (Roxb. ex Fleming) Wall. through the application of differential staining techniques (using orcein, CMA, and DAPI) were studied. After applying orcein staining, the interphase nuclei of this species exhibited a ‘simple chromocenter type’ with numerous small heterochromatin blocks and the prophase chromosomes were ‘interstitial type’. The karyotype analysis revealed that this species possesses diploid chromosome number of 2n = 22, with all metacentric chromosomes. The total length of the chromosome complement in the diploid set was 29.91 μm. A total of four CMA-positive bands were observed in this species whereas seven bands were found after DAPI-staining. Heteromorphicity was observed in the DAPI stained metaphase stage indicating the probable occurrences of chromosomal aberrations such as deletion and inversion. The karyotype profile of H. antidysenterica obtained from orcein and fluorescent banding was determined for the first time in Bangladesh. Bangladesh J. Bot. 52(4): 1085-1089, 2023 (December)

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