复合菌剂在麝香瓜病原体生物防治中的潜力

Pub Date : 2023-12-31 DOI:10.3329/bjb.v52i4.70603
R. Fu, Hong Zhang, Tieqi Xia, Xue Yang, Dingwang, Wu-ling Chen
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引用次数: 0

摘要

从麝香瓜温室的土壤中筛选出细菌菌株 TG6,该菌株能有效抑制麝香瓜的褐斑病菌(Sphaerotheca fuliginea)、氧孢镰刀菌(Fusarium oxysporum)、瓜疫霉(Phytophthora melonis)和立方假包囊病菌(Pseudoperonospora cubensis)的生长。通过形态学、生理学、生物化学和分子研究,确定 TG6 为枯草芽孢杆菌。以 TG6 为原始菌株,通过氦氖激光诱变获得了 TG67 和 TG69。按照 1:1 的比例制备了微生物复合拮抗剂菌株 TG67 和 TG69,并将其应用于温室甜瓜的生物防治。西瓜田间试验表明,该杀菌剂可有效控制温室西瓜各种病害的发生。孟加拉国植物学报52(4):1047-1053, 2023 (December)
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Potential of compound bacterial agent in the biocontrol of muskmelon pathogens
The bacterial strain TG6 was screened from the soil of a muskmelon greenhouse, which could effectively inhibit the growth of Sphaerotheca fuliginea, Fusarium oxysporum, Phytophthora melonis and Pseudoperonospora cubensis of muskmelons. TG6 was identified as Bacillus subtilis through morphological, physiological, biochemical and molecular studies. Taking TG6 as the original strain, TG67 and TG69 were obtained by He-Ne laser mutagenesis. The microbial compound antagonist strain TG67 and TG69 was prepared in the ratio of 1:1 and applied to the biological control of greenhouse melon. Muskmelon field experiments showed that the bactericide could effectively control the occurrence of various diseases of muskmelon in greenhouse. Bangladesh J. Bot. 52(4): 1047-1053, 2023 (December)
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