{"title":"Workshop on future directions for plant research at NASA: a report.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>NASA's Fundamental Space Biology Division and the Advanced Human Support Technology Program convened a workshop in December 2003 [correction of 2004] at Kennedy Space Center, Florida, to chart future directions for plant research at NASA. The purpose of the workshop was to bring together key managers and principal investigators in NASA's plant research community as well as non-NASA funded researchers to formulate a strategy to guide future plant research for the Office of Biological and Physical Research (OBPR). Subsequent to the workshop, on Wednesday, January 14, 2004, President George W. Bush announced a proposal for NASA to go back to the moon and later send a manned mission to Mars. The following is a summary of workshop recommendations provided by the plant research community within the framework of the new exploration vision as set for by NASA.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25271287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gautam Biswas, Eric-Jan Manders, John Ramirez, Nagabhusan Mahadevan, Sherif Abdelwahed
{"title":"Online model-based diagnosis to support autonomous operation of an advanced life support system.","authors":"Gautam Biswas, Eric-Jan Manders, John Ramirez, Nagabhusan Mahadevan, Sherif Abdelwahed","doi":"10.3727/154296604774808892","DOIUrl":"https://doi.org/10.3727/154296604774808892","url":null,"abstract":"<p><p>This article describes methods for online model-based diagnosis of subsystems of the advanced life support system (ALS). The diagnosis methodology is tailored to detect, isolate, and identify faults in components of the system quickly so that fault-adaptive control techniques can be applied to maintain system operation without interruption. We describe the components of our hybrid modeling scheme and the diagnosis methodology, and then demonstrate the effectiveness of this methodology by building a detailed model of the reverse osmosis (RO) system of the water recovery system (WRS) of the ALS. This model is validated with real data collected from an experimental testbed at NASA JSC. A number of diagnosis experiments run on simulated faulty data are presented and the results are discussed.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"21-37"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/154296604774808892","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25099142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myron T La Duc, Randall Sumner, Duane Pierson, Parth Venkat, Kasthuri Venkateswaran
{"title":"Evidence of pathogenic microbes in the International Space Station drinking water: reason for concern?","authors":"Myron T La Duc, Randall Sumner, Duane Pierson, Parth Venkat, Kasthuri Venkateswaran","doi":"10.3727/154296604774808883","DOIUrl":"https://doi.org/10.3727/154296604774808883","url":null,"abstract":"<p><p>Molecular analyses were carried out on four preflight and six postflight International Space Station (ISS)-associated potable water samples at various stages of purification, storage, and transport, to ascertain their associated microbial diversities and overall microbial burdens. Following DNA extraction, PCR amplification, and molecular cloning procedures, rDNA sequences closely related to pathogenic species of Acidovorax, Afipia, Brevundimonas, Propionibacterium, Serratia, and others were recovered in varying abundance. Retrieval of sequences arising from the iodine (biocide)-reducing Delftia acidovorans in postflight waters is also of concern. Total microbial burdens of ISS potable waters were derived from data generated by an ATP-based enumeration procedure, with results ranging from 0 to 4.9 x 10(4) cells/ml. Regardless of innate biases in sample collection and analysis, such circumstantial evidence for the presence of viable, intact pathogenic cells should not be taken lightly. Implementation of new cultivation approaches and/or viability-based assays are requisite to confirm such an occurrence.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/154296604774808883","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25099143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corinne J Rutzke, Raymond P Glahn, Michael A Rutzke, Ross M Welch, Robert W Langhans, Louis D Albright, Gerald F Combs, Raymond M Wheeler
{"title":"Bioavailability of iron from spinach using an in vitro/human Caco-2 cell bioassay model.","authors":"Corinne J Rutzke, Raymond P Glahn, Michael A Rutzke, Ross M Welch, Robert W Langhans, Louis D Albright, Gerald F Combs, Raymond M Wheeler","doi":"10.3727/154296604774808900","DOIUrl":"https://doi.org/10.3727/154296604774808900","url":null,"abstract":"<p><p>Spinach (Spinacia oleracea) cv Whitney was tested for iron bioavailabilty using an in vitro human intestinal cell culture ferritin bioassay technique previously developed. Spinach was cultured in a growth chamber for 33 days, harvested, and freeze-dried. Total iron in the samples was an average of 71 micrograms/g dry weight. Spinach was digested in vitro (pepsin and 0.1 M HCl followed by pancreatin and 0.1 M NaHCO3) with and without the addition of supplemental ascorbic acid. Caco-2 cell cultures were used to determine iron bioavailability from the spinach mixtures. Production of the iron-binding protein ferritin in the Caco-2 cells showed the supplemental ascorbic acid doubled bioavailability of iron from spinach. The data show fresh spinach is a poor source of iron, and emphasize the importance of evaluation of whole meals rather than single food items. The data support the usefulness of the in vitro/Caco-2 cell ferritin bioassay model for prescreening of space flight diets for bioavailable iron.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"7-14"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/154296604774808900","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25271288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vadim Y Rygalov, Philip A Fowler, Raymond M Wheeler, Ray A Bucklin
{"title":"Water cycle and its management for plant habitats at reduced pressures.","authors":"Vadim Y Rygalov, Philip A Fowler, Raymond M Wheeler, Ray A Bucklin","doi":"10.3727/154296604774808865","DOIUrl":"https://doi.org/10.3727/154296604774808865","url":null,"abstract":"<p><p>Experimental and mathematical models were developed for describing and testing temperature and humidity parameters for plant production in bioregenerative life support systems. A factor was included for analyzing systems operating at low (10-101.3 kPa) pressure to reduce gas leakage and structural mass (e.g., inflatable greenhouses for space application). The expected close relationship between temperature and relative humidity was observed, along with the importance of heat exchanger coil temperature and air circulation rate. The presence of plants in closed habitats results in increased water flux through the system. Changes in pressure affect gas diffusion rates and surface boundary layers, and change convective transfer capabilities and water evaporation rates. A consistent observation from studies with plants at reduced pressures is increased evapotranspiration rates, even at constant vapor pressure deficits. This suggests that plant water status is a critical factor for managing low-pressure production systems. The approach suggested should help space mission planners design artificial environments in closed habitats.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"49-59"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/154296604774808865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25099144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of drying on nitrification activity in zeoponic medium used for long-term space missions.","authors":"R L McGilloway, R W Weaver","doi":"10.3727/154296604774808874","DOIUrl":"https://doi.org/10.3727/154296604774808874","url":null,"abstract":"<p><p>One component of a proposed life support system is the use of zeoponic substrates, which slowly release NH4+ into \"soil\" solution, for the production of plants. Nitrifying bacteria that convert NH4+ to NO3- are among the important microbial components of these systems. Survival of nitrifying bacteria in dry zeoponic substrates is needed, because the substrate would likely be stored in an air-dry state between croppings. Substrate was enriched for nitrifying bacteria and allowed to air-dry in a laminar flow hood. Stored substrate was analyzed for nitrifier survivability by measuring nitrifier activity at the beginning, 3 days, 1, 2, and 3 weeks. After rewetting, activity was approximately 9 micrograms N g-1 h-1 regardless of storage time. Nitrification rates did not decrease during storage. It seems unlikely that drying between plantings would result in practical reductions in nitrification, and reinoculation with nitrifying bacteria would not be necessary.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"10 1","pages":"15-9"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/154296604774808874","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25099141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu A Berkovich, N M Krivobok, S M Krivobok, V V Matusevich, V S Soldatov
{"title":"Development of a root feeding system based on a fiber ion-exchange substrate for space plant growth chamber \"Vitacycle\".","authors":"Yu A Berkovich, N M Krivobok, S M Krivobok, V V Matusevich, V S Soldatov","doi":"10.3727/1542966034605261","DOIUrl":"https://doi.org/10.3727/1542966034605261","url":null,"abstract":"<p><p>Selecting a plant root nutrient delivery system is one of the key aspects of designing root modules for space plant growth chambers. This article examines a number of the nutrient delivery systems and shows the most suitable technique for providing nutrients to roots in microgravity, which to date are ion-exchange artificial soils. In addition, this article characterizes the ion composition and hydrophysical parameters of a new Russian artificial ion charged fiber substrate, BIONA-V3. The BIONA-V3 substrate is comprised of ion-exchange resin fibers. The experimental data concerning the effects of anionic and cationic components on plant biomass is presented. Preliminary experiments with BIONA-V3 showed that 1 kg of dry BIONA-V3 produces up to 2.4 kg (fresh mass) of cabbage leaf or 180 g of dry plant mass per 1 dm3 of the substrate. Therefore, the root zone volume can be as small as 120 cm3 per plant. Further optimizing the nutrient composition of the resin fibers can increase space plant growth chamber productivity.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"9 1-2","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/1542966034605261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24088043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Removal of sodium chloride from human urine via batch recirculation electrodialysis at constant applied voltage.","authors":"Nilda E Gordils-Striker, Guillermo Colon","doi":"10.3727/1542966034605298","DOIUrl":"https://doi.org/10.3727/1542966034605298","url":null,"abstract":"<p><p>The removal of sodium chloride (NaCl) from human urine using a six-compartment electrodialysis cell with batch recirculation mode of operation for use in advanced life support systems (ALSS) was studied. From the results obtained, batch recirculation at constant applied voltage yields high values (approximately 94% of NaCl removal. Based on the results, the initial rate of NaCl removal was correlated to a power function of the applied voltage: -r=2.0 x 10(-4)E(3.8). With impedance spectroscopy methods, it was also found that the anion membranes were more affected by fouling with an increase of the ohmic resistance of almost 11% compared with 7.4% for the cationic ones.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"9 1-2","pages":"47-57"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/1542966034605298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24088041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differential expression of hsp60 and hsp70 associated with cytotoxicity under microgravity conditions.","authors":"Tie Hong, Guang-Bi Jin, Shigefumi Cho, Takao Kobayashi, Fumiko Yasuno, Yoshihiko Aikawa, Jong-Chol Cyong","doi":"10.3727/1542966034605315","DOIUrl":"https://doi.org/10.3727/1542966034605315","url":null,"abstract":"<p><p>It is known that space flight affects T lymphocyte function in both humans and animals, but there have been no papers dealing with the effect of microgravity conditions for a very short time (i.e., only 10 s). In the present study, the effect of very short time microgravity on the cytotoxicity and surface markers of human activated T lymphocytes, in vitro, was investigated using the drop-shaft type of microgravity experiment system. The levels of heat shock protein 60 and 70 (hsp60 and hsp70) were also quantified in cells exposed to these microgravity conditions. The results showed that not only the cytotoxicity but also the hsp60 levels were remarkably reduced under these conditions.</p>","PeriodicalId":86963,"journal":{"name":"Habitation (Elmsford, N.Y.)","volume":"9 1-2","pages":"41-6"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3727/1542966034605315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24088042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}