Tsuneo A. Takahashi, M. Hosoda, Y. Mogi, Hitoshi Kuroki, Shinichiro Kuroda, Mineo Sado, Sadayoshi, Sekiguchi
{"title":"A NEW POROUS POLYURETHANE FILTER TO REMOVE LEUKOCYTES FROM PLATELET CONCENTRATES","authors":"Tsuneo A. Takahashi, M. Hosoda, Y. Mogi, Hitoshi Kuroki, Shinichiro Kuroda, Mineo Sado, Sadayoshi, Sekiguchi","doi":"10.3925/jjtc1958.37.24","DOIUrl":"https://doi.org/10.3925/jjtc1958.37.24","url":null,"abstract":"A NEW POROUS POLYURETHANE FILTER TO REMOVE LEUKOCYTES FROM PLATELET CONCENTRATES Tsuneo A. Takahashi, Mani Hosoda, Yuko Mogi, Hitoshi Kuroki, Shinichiro Kuroda, Mineo Sado and Sadayoshi Sekiguchi Recent studies have shown that the transfusion of leukocyte-depleted platelet concentrates (PCs) could reduce HLA alloimmunization and platelet refractoriness. The authors developed a new porous polyurethane filter for the depletion of leukocytes from PCs and evaluated its efficiency in removing leukocytes. The new filter, the Imugard PL, is designed to be used without priming at the patient's bedside. The leukocyte removal rate, platelet recovery and platelet functions were studied. The leukocyte removal rate was higher than 99. 9% when a pool of 10 units of PCs stored for 1-3 days or apheresis PCs similar in number to 15 units of PCs stored for 1 day was filtered. The platelet recovery was higher than 92. 5% for the apheresis PCs and 96% for the pools of 10 PCs. The morphology and functions of platelets were not affected by the filtration except for a slight increase of the response to aggregation and a decrease of mean platelet volume after filtration. The Imugard PL is effective in removing leukocytes without losing platelets and should help to improve platelet transfusion therapy.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70311436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"FOUR EPITOPES ON THE RH ANTIGEN E RECOGNIZED BY HUMAN MONOCLONAL ANTIBODIES","authors":"Toru, Miyazaki, Ku-Won, Kwon, K. Yamamoto, Yuichi, Toner, Hiromi, Ihara, Toshiaki, Katol, Hisami, Ikeda, Sadayoshi, Sekiguchi","doi":"10.3925/jjtc1958.39.840","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.840","url":null,"abstract":"E antigen of the human Rh blood group system was serologically analyzed with five human monoclonal anti-E antibodies including a newly developed HMR37 antibody. Four different reaction patterns of these monoclonal antibodies were observed by agglutination test and fluorescence flowcytometry with six Evariant cells showing incompatible reaction between monoclonal anti-E and conventional polyclonal anti-E. The mutual inhibition study using fluorescence-labelled antibodies also supported that four epitopes recognized by the monoclonal antibodies are on the normal EE cells. These results indicate that at least four distinct epitopes are present on the E antigen, and suggest that anti-E antibody production may occur in E-positive individuals partially lacking the E epitope(s).","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70319157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INVESTIGATION OF ENGRAFTMENT AND LEUKEMIA RELAPSE AFTER BONE MARROW TRANSPLANTATION, USING A VARIABLE NUMBER OF TANDEM REPEAT (VNTR) AND 6 RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs)","authors":"Yokiko, Ohel, Shunro, Kail, Hiroshi, Haral, Yoshiaki, Tsujino, Takashi, Kamiya, Masuji, Yamamoto, Hiroyoshi, Wada, Atsushi, Nishinakagawa, Yoshihiro, Jun-ichi, Furuyama","doi":"10.3925/jjtc1958.41.198","DOIUrl":"https://doi.org/10.3925/jjtc1958.41.198","url":null,"abstract":"Tetsuo Kooritanil), Akira Kokubunjil), Kiyosuke Taniwakil), Masao Yamaguchil), Maki Itol), Kazuaki Ananl), Mahito Misawa1), Yokiko Ohel), Shunro Kail), Hiroshi Haral), Yoshiaki Tsujino2), Takashi Kamiya2), Masuji Yamamoto2) Hiroyoshi Wada2), Atsushi Nishinakagawa3), Yoshihiro Yamamoto3) and Jun-ichi Furuyama3) Departments of Transfusion Medicine', Pediatrics2) and Genetics3), Hyogo College of Medicine","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70322865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sachiyo, Nishida, Y. Fujimura, H. Fukui, M. Nakajima, Shigeru, Kuwahara, Akira, Yoshioka, Yasuhiro, Matsuzuka, Masahito, Sugimura
{"title":"A DENTAL SURGERY OF 2 RADICULAR CYST IN A PATIENT WITH BERNARD-SOULIER SYNDROME USING PLATELET CONCENTRATES PREPARED BY A CELL SEPARATOR SPECTRA (COBE)","authors":"Sachiyo, Nishida, Y. Fujimura, H. Fukui, M. Nakajima, Shigeru, Kuwahara, Akira, Yoshioka, Yasuhiro, Matsuzuka, Masahito, Sugimura","doi":"10.3925/jjtc1958.37.424","DOIUrl":"https://doi.org/10.3925/jjtc1958.37.424","url":null,"abstract":"Bernard-Soulier syndrome is a congential bleeding diathesis characterized by an autosomal recessive inheritance and appearance of dysfunctional giant platelet in circulation. We describe here a successful treatment of 2 radicular cyst and dental extraction in that patient under hemostatic control by the infusion of HLA-matched platelet concentrates (PCs), which were prepared by a newly introduced cell separator named SPECTRA (COBS). The PCs prepared by SPECTRA had a great advantage in terms of reduction of contaminated white blood cell count, as compared with those prepared by other cell separators. Infusion of the SPECTRA-PCs to the patient showed dramatic effect on the shortening of bleeding time, and no excess bleeding was observed during operation. Despite these clinical improvement, ristocetin-induced platelet aggregation (RIPA) defective in the patient remained almost unchanged. To explore this conflicting result, studies on, RIPA were performed in vitro by mixing platelet and plasmas from the patient or normal individual. From this experiment, it became clear that defective RIPA found in the patient even after infusion of normal PC is mostly attributable to the presence of dysfunctional giant platelet, and not to the presence of plasma factor including inhibitor to platelet membrance components.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70312497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Kosugi, Y. Tomiyama, H. Kashiwagi, Y. Kanayama, Y. Kurata, Y. Matsuzawa
{"title":"COMPARISON OF THE SENSITIVITY OF ABC-MACE, MACE, MAIPA AND FCM FOR THE DETECTION OF ANTI-PLATELET ALLOANTIBODIES","authors":"S. Kosugi, Y. Tomiyama, H. Kashiwagi, Y. Kanayama, Y. Kurata, Y. Matsuzawa","doi":"10.3925/jjtc1958.39.720","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.720","url":null,"abstract":"COMPARISON OF THE SENSITIVITY OF ABC-MACE, MACE, MAIPA AND FCM FOR THE DETECTION OF ANTI-PLATELET ALLOANTIBODIES Satoru Kosugi, Yoshiaki Tomiyama, Hirokazu Kashiwagi, Yoshio Kanayama, Yoshiyuki Kurata1) and Yuji Matsuzawa The Second Department of Internal Medicine, Osaka University Medical School 1) Department of Blood Transfusion, Osaka University Hospital Recently, antigen-specific assays for detecting anti-platelet alloantibodies have been available: immunobead assay, modified antigen capture ELISA (MACE) and monoclonal antibody-specific immobilization of platelet antigens (MAIPA). These assays are based on essentially the same principle. In this study, using anti-HPA-la and anti-HPA-4a antibody, we have compared the sensitivity of MACE using avidin-biotin-complex method (ABC-MACE), MACE, MAIPA, and flowcytometry (FCM) as a conventional method. The sensitivity of MACE for detecting anti-HPA-la antibody was twofold better than that of MAIPA, while the sensitivity of MAIPA for detecting anti-HPA-4a antibody was twofold better than that of MACE. Using ABC-method, the sensitivity of MACE for detecting antiHPA-la and anti-HPA-4a increased 8-fold and 64-fold, respectively. In comparison with FCM, ABCMACE showed almost the same sensitivity for detecting anti HPA-4a antibody, and showed better sensitivity for detecting anti HPA-la antibody. These data demonstrate that ABC-MACE is much more sensitive than MACE or MAIPA for detecting anti-platelet alloantibodies, and that the sensitivity of ABC-MACE is comparable to that of FCM.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70318380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hino, A. Kobayashi, Michiko Imamoto, M. Endo, Hitomi Tanaka, Nobuyuki Yokokawa, I. Nakao, M. Shimizu
{"title":"SCREENIGN OF VOLUNTARY BLOOD DONORS BY HCV RELATED ANTIBODY TEST KIT (IMUCHECK)","authors":"S. Hino, A. Kobayashi, Michiko Imamoto, M. Endo, Hitomi Tanaka, Nobuyuki Yokokawa, I. Nakao, M. Shimizu","doi":"10.3925/jjtc1958.39.726","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.726","url":null,"abstract":"SCREENIGN OF VOLUNTARY BLOOD DONORS BY HCV RELATED ANTIBODY TEST KIT (IMUCHECK) Satoru Hino1, Akira Kobayashi1), Michiko Imamoto1), Masahiro Endo1, Hitomi Tanaka1), Nobuyuki Yokokawa1, Isao Nakao1 and Masaru Shimizu2) 1) Tokyo Komagome Red Cross Blood Center 2) Department of Transfusion Medicine, Tokyo Women's Medical College HCV related antibody was screened among 3291 voluntary blood donors by two test kits (Imucheck for screening and Imunojudge for immunobloting) using two recombinant antigens (C7 in NS3 and C 11 in core region) cloned from the plasma of Japanese non-A, non-B hepatitis patient. The positive rates of Imucheck and PHA (DAINABOT) were 3.04% and 0.55%, respectively. 88 out of 100 Imucheck positive samples were tested by Imunojudge, which resulted in 53 (60.2%) samples positive to C7 and/or C 11 antigen; 12 samples were positive to C7, 23 to C 11 and 18 to both. HCV-RNA was detected in 11 samples positive with Imunojudge by PCR using 5' non-coding primer, all of which showed high C.LO. above 8.00 by Imucheck and had both C7 and C11 antibodies by Imunojudge. These samples with HCV-RNA showed more than 212 in PHA titer and RIBA-II positive. Imucheck detected 5.5 times more positive samples than PHA did, but one third of them was considered non-specific reaction by Imunojudge. The samples which had both C 7 and C 11 antibodies were mostly PHA positive, and covered the all samples with HCV-RNA. Although Imucheck and Imunojudge would be useful in screening blood donors for HCV related antibodies, it remains to be clarified how to consider only either C7 or C 11 positive samples.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70318446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFICACY OF TWO ROUTES OF r-HuEPO ADMINISTRATION IN PREDEPOSIT AUTOLOGOUS BLOOD DONATION","authors":"Yuko, Hitomi, Hiroo, Maeda, Ranko, Hirata, Hiroshi, Tohyama, Nobuyuki, Tsuzuki","doi":"10.3925/jjtc1958.41.605","DOIUrl":"https://doi.org/10.3925/jjtc1958.41.605","url":null,"abstract":"Predeposit autologous blood donation (PABD) with recombinant human erythropoietin (r-HuEPO) has recently come into widespread use. To determine the optimal route of r-HuEPO administration for PABD, we investigated the effects of r-HuEPO by different routes on predeposit autologous 1,200ml blood donation courses in 71 patients undergoing elective orthopedic surgery. The patients were divided into three groups as follows: 36 patients were given r-HuEPO two or three times a week intravenously, 21 were given r-HuEPO once a week subcutaneously, and 14 without r-HuEPO served as controls. The increase in hemoglobin concentration in both r-HuEPO administration groups (3.46+1.16g/dl intravenous; 2.40+0.76g/dl subcutaneous) was significantly greater than that in the controls (1.47+1.04g/dl). To further compare the effects of r-HuEPO by both routes, regression analysis between increase in hemoglobin concentration per week period and total dose of r-HuEPO divided by body weight was done. The hemoglobin increase (y) demonstrated a significant positive linear correlation with r-HuEPO dose (x) in both groups (y=5.0X10-4+0.28, r=0.86 for the intravenous group, y=4.7X10-4+ 0.37, r=0.55 for the subcutaneous group), with the two lines revealing no significant difference from each other. In conclusion, both routes of r-HuEPO administration given under the same total dose are equally efficacious in raising hemoglobin concentration level.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70324067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SAFE (The Saline versus Albumin Fluid Evaluation) 試験","authors":"S. Finfer","doi":"10.3925/JJTC1958.52.19","DOIUrl":"https://doi.org/10.3925/JJTC1958.52.19","url":null,"abstract":"","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"52 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"2006-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70337335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Chiyoda, Hidetaka Oda, Tamaka Kotani, E. Kusaba, T. Kusano
{"title":"THE EFFECT OF A NATIONWIDE PREVENTION PROGRAM FOR MOTHER-TO-INFANT INFECTION BY HBV EVALUATED BY VIRAL MARKERS IN BLOOD DONORS IN NAGASAKI PREFECTURE","authors":"S. Chiyoda, Hidetaka Oda, Tamaka Kotani, E. Kusaba, T. Kusano","doi":"10.3925/jjtc1958.52.52","DOIUrl":"https://doi.org/10.3925/jjtc1958.52.52","url":null,"abstract":"Nagasaki Prefecture is a highly endemic area for hepatitis B virus (HBV). In recent years, we have identified a decrease in the incidence of positivity for hepatitis B surface antigen (HBsAg). In 1985, the Government of Japan started a nationwide prevention program for mother-to-infant infection by HBV. As blood donor is acceptable from age 16, a portion of the people who owed this program became to age with approval of blood donation. We screened 291, 587 serum samples from voluntary blood donors (170, 366 males and 121, 221 females) between 16 and 69 years of age who first visited Nagasaki and Sasebo Red Cross Blood Center from June 1990 to December 2004. The positive rate for HBsAg among blood donors by year of birth decreased from 1.67 to 0% over the years 1929 to 1988 (test of trend; p < 0. 001). No donor born from 1986 to 1988 was positive, although a decrease in the positive rate was recognized before 1985. This may be because in 1985, the Government of Japan started a prevention program for mother-to-infant infection by HBV. Shiraki has reported that this program was very effective. The present study involving the analysis of data among blood donors provides further evidence for the high efficacy of this program.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"52 1","pages":"52-56"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70337460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}