Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion最新文献_第3页

EVALUATION OF AN APPROPRIATE SIZE OF UNRELATED DONOR POOL FOR HLA-MATCHED PLATELET TRANSFUSION AND BONE MARROW TRANSPLANTATION 评价hla匹配血小板输注和骨髓移植的非亲属供体池的适当大小

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.38.475

K. Takahashi, K. Tokunaga, C. Uchikawal, T. Juji, T. Akaza, K. Umezawa, N. Endoh, H. Kamata, H. Tsuchida, K. Fujino, I. Watanabe

引用次数: 0

HEPATITIS B VIRUS (HBV) CARRIER CLINIC -10 YEAR'S EXPERIENCE IN A BLOOD CENTER- 乙型肝炎病毒(hbv)携带者门诊-在血液中心工作10年的经验-

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.41.364

Tomoko, Kimoto, Hiromi, Ihara, Toshiaki, Kato, Toshie, Nakasel, Hisami, Ikeda, Shinichi, Sakamoto

{"title":"HEPATITIS B VIRUS (HBV) CARRIER CLINIC -10 YEAR'S EXPERIENCE IN A BLOOD CENTER-","authors":"Tomoko, Kimoto, Hiromi, Ihara, Toshiaki, Kato, Toshie, Nakasel, Hisami, Ikeda, Shinichi, Sakamoto","doi":"10.3925/jjtc1958.41.364","DOIUrl":"https://doi.org/10.3925/jjtc1958.41.364","url":null,"abstract":"We established a special clinic (in 1985) for asymptomatic HBV carriers detected through blood donation. The main purpose of this clinic was the early detection and treatment of chronic hepatitis. In 10 years 3, 367 notified carriers detected from over two million blood donors visited our clinic after attending an orientation seminar. The HBsAg-positive rate among first-time donors decreased from 3.0% to 1.2% during these 10 years. The overall HBeAg-positive rate in clinic visitors was 13.2%, and decreased with age. Elevated GPT levels were seen in 6.0% of clinic visitors, with a higher rate in HBeAgpositive carriers. HBsAg as tested with EIA disappeared in 40 cases (1.2%). HBeAg disappeared in 23 cases with elevated GPT. HCV antibody was detected in 16 cases. Carriers with elevated GPT or space-occupying lesions in the liver as detected by echography were referred to specialized hospitals. Of these, 166 (4.9%) cases were diagnosed as HBV-related liver disease, including 5 cases of hepatoma. Thus, regular follow-up in our HBV carrier clinic strongly contributed to the medical care of asymptomatic HBV carriers.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70323708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HEMATOLOGICAL, METABOLIC AND RHEOLOGICAL CHARACTERISITICS OF ERYTHROCYTES IN RECOMBINANT HUMAN ERYTHROPOIETIN-ADMINISTERED RAT I. Hematological and Metabolic Properties 重组人促红细胞生成素大鼠红细胞的血液学、代谢和流变学特征1 .血液学和代谢特性

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.35.627

N. Maeda

{"title":"HEMATOLOGICAL, METABOLIC AND RHEOLOGICAL CHARACTERISITICS OF ERYTHROCYTES IN RECOMBINANT HUMAN ERYTHROPOIETIN-ADMINISTERED RAT I. Hematological and Metabolic Properties","authors":"N. Maeda","doi":"10.3925/jjtc1958.35.627","DOIUrl":"https://doi.org/10.3925/jjtc1958.35.627","url":null,"abstract":"HEMATOLOGICAL, METABOLIC AND RHEOLOGICAL CHARACTERISITICS OF ERYTHROCYTES IN RECOMBINANT HUMAN ERYTHROPOIETIN-ADMINISTERED RAT I. Hematological and Metabolic Properties Nobuji Maeda Department of Physiology, School of Medicine, Ehime University Recombinant human erythropoietin (rhEPO; 180 or 1800 IU/kg) was intravenously administered daily to 6 weeks-old rats for 1 or 2 weeks, and hematological and metabolic properties of the erythrocytes were examined. (1) A considerable polycythemia with reticulocytosis was induced in a dose-dependent manner accompanying increase in MCV and decrease in MCHC. The erythrocytes were inhomogeneous in their density, and the density distribution was shifted towards low specific gravity. Probably, young erythrocytes were increased in the circulation by rhEPO administration. (2) Slight augmentation of intracellular ATP and GTP contents was detected, but 2, 3-DPG content was not altered. (3) Upon the long-term administration of rhEPO, intracellular hemoglobin content was decreased, and the reticulocytosis was reduced.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70307119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CLINICAL SIGNIFICANCE OF SERIAL EXAMINATION OF ANTI HUMAN IMMUNODEFICIENCY VIRUS ( HIV ) ANTIBODY AND HIV ANTIGEN IN HEMOPHILIACS WITH HIV INFECTION 血友病合并HIV感染患者抗人类免疫缺陷病毒(HIV)抗体和HIV抗原系列检测的临床意义

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.34.332

Sayuri Odajima, J. Matsuda, T. Hamauzu, N. Saito, T. Kinoshita, T. Abe

{"title":"CLINICAL SIGNIFICANCE OF SERIAL EXAMINATION OF ANTI HUMAN IMMUNODEFICIENCY VIRUS ( HIV ) ANTIBODY AND HIV ANTIGEN IN HEMOPHILIACS WITH HIV INFECTION","authors":"Sayuri Odajima, J. Matsuda, T. Hamauzu, N. Saito, T. Kinoshita, T. Abe","doi":"10.3925/jjtc1958.34.332","DOIUrl":"https://doi.org/10.3925/jjtc1958.34.332","url":null,"abstract":"CLINICAL SIGNIFICANCE OF SERIAL EXAMINATION OF ANTI HUMAN IMMUNODEFICIENCY VIRUS (HIV) ANTIBODY AND HIV ANTIGEN IN HEMOPHILIACS WITH HIV INFECTION Sayuri Odajima, Juzo Matsuda, Toshikazu Hamauzu, Noriko Saito, Tadatoshi Kinoshita and Takeshi Abe Department of Medicine, Teikyo University School of Medicine We tested for the anti HIV antibody and the HIV antigen in the sera of 81 hemophiliacs (A 68 cases, B 13 cases), who have been under treatment in our department. The anti HIV antibody was found to be present in 44.3% of the cases, including 7 cases who later changed to positive, and 6 with HIV antigen. In the sera of those 7 seroconverted cases, although the HN antigen was negative, the p24 antibody was detected by the Western blotting method and the gp41 antibody was detected by the Confirmatory EIA (ENVACORE, Abbott Lab., USA) earlier than by the immunofluorescence method and conventional ELISA. Moreover, in 4 cases who later developed AIDS, HIV turned out positive concurrently with or shortly after the enfeeblement and/or disappearance of the p24 antibody which was associated with a progressive decrease in the CD4/8 ratio of lymphocytes and the development of recurrent oppotunistic infections. Based on these results, it is suggested that the periodical examination for anti p24 and gp41 antibodies and HIV itself might be clinically useful for predicting the development of AIDS and thustreating them at an early stage.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70303933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ENRICHMENT OF CD34 POSITIVE CELLS IN HUMAN UMBILICAL CORD BLOOD AND IN VITRO EXPANSION OF HEMATOPOIETIC PROGENITOR CELLS 人脐血中cd34阳性细胞的富集及造血祖细胞的体外扩增

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.40.694

Emi Kadowaki, Tsuneo A. Takahashi, Y. Mogi, A. Sohma, Y. Yorozu, A. Kohgo, M. Yamaguchi, Toshinobu Tanaka, S. Fujimoto, S. Sekiguchi

引用次数: 0

APPLICATION OF A LOW SERUM CULTURE MEDIUM FOR CELL DENSE CULTURE OF LYMPHOKINE ACTIVATED KILLER (LAK) CELLS 低血清培养基在淋巴因子活化杀伤细胞(lak)细胞密集培养中的应用

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.36.387

Y. Nakamura, Satomi Wakamatsu, N. Kobayashi, T. Noto, Y. Tokuda, T. Tajima

{"title":"APPLICATION OF A LOW SERUM CULTURE MEDIUM FOR CELL DENSE CULTURE OF LYMPHOKINE ACTIVATED KILLER (LAK) CELLS","authors":"Y. Nakamura, Satomi Wakamatsu, N. Kobayashi, T. Noto, Y. Tokuda, T. Tajima","doi":"10.3925/jjtc1958.36.387","DOIUrl":"https://doi.org/10.3925/jjtc1958.36.387","url":null,"abstract":"APPLICATION OF A LOW SERUM CULTURE MEDIUM FOR CELL DENSE CULTURE OF LYMPHOKINE ACTIVATED KILLER (LAK) CELLS Yoshihiko Nakamura, Satomi Wakamatsu, Nobumasa Kobayashi and Katsuto Watanabe Blood Transfusion Service Center Takashi Noto, Yutaka Tokuda, Tomoo Tajima and Toshio Mitomi The Second Department of Surgery, School of Medicine, Tokai University Lymphokine activated killer (LAK) cells were cultured in low serum culture medium that was newly developed. The medium was composed of three basal media, i. e. Dulbecco's modified eagle (DME), F-12 (Ham) and RPMI-1640, and it was enriched with other supplements. The medium was applied with 1% gelatin and 3% AB serum for CRTC (concentrate rotary tissue culture) system, or with 1% AB serum for hollow-fiber system in LAK cells culture. It was demonstrated that the medium can be used to proliferate LAK cells to about thirty-fold the initial cell number with CRTC system, or to twenty one-fold with hollow-fiber system. Therefore, this medium can be applied for these culture systems to obtain a large number of LAK cells in low serum concentration.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70307896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CORRELATION BETWEEN HUMAN T CELL LEUKEMIA VIRUS TYPE 1 INFECTION AND HEPATITIS VIRUS INFECTION 人1型t细胞白血病病毒感染与肝炎病毒感染的相关性

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.39.911

S. Yakabi, H. Zukeran, Y. Miyagi, R. Taira, H. Sakugawa, Keishun Koja

{"title":"CORRELATION BETWEEN HUMAN T CELL LEUKEMIA VIRUS TYPE 1 INFECTION AND HEPATITIS VIRUS INFECTION","authors":"S. Yakabi, H. Zukeran, Y. Miyagi, R. Taira, H. Sakugawa, Keishun Koja","doi":"10.3925/jjtc1958.39.911","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.911","url":null,"abstract":"CORRELATION BETWEEN HUMAN T CELL LEUKEMIA VIRUS TYPE 1 INFECTION AND HEPATITIS VIRUS INFECTION Shizuko Yakabi, Hiroki Zukeran, Yasuhiro Miyagi, Reiko Taira, Hiroshi Sakugawa and Keishun Koja Department of Blood Transfusion, Ryukyu University Hospital Kazuhiko Akamine, Tomio Uezu, Mono Kina and Keishou Ohmine Okinawa Red Cross Blood Center It is well known that human T-cell leukemia virus type 1 (HTLV-1) infection is contributed to deficient immunity. Some association between HTLV-1 infection and hepatitis virus infection were investigated, particularly the effect of HTLV-1 infection on the acquisition of hepatitis B and C virus carrier state, and on liver function in hepatitis virus carriers. The subjects consisted of 945 HTLV-1 positive donors aged 16-64 (mean+S. D.=37.4+11) and 2,000 negative controls aged 16-64 (mean+S. D.=30.4+10). The prevarence of hepatitis B surface antigen in HTLV-1 positive donors and negative controls were 1.9% and 1.6%, respectively. However, there was no statistically significant difference. On the other hand, the prevalence of antibody to hepatitis C virus in HTLV-1 positive groups was 2.5% and significantly higher than in negative controls being 0.4%. Frequency of donors with high value of GPT was not different between HTLV-1 positive group and negative. In conclusion, HTLV-1 infection may not affect on the acquisition of HBV carrier state and liver function. However, an association between HTLV-1 and HCV infection was suggested.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70319348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF PLATELET SHAPE BY A STOP AND FLOW METHOD USING AGGREGOMETER : Application to Quality Control of Stored Platelet Concentrates 聚合计停流法评价血小板形态:在贮存血小板浓缩物质量控制中的应用

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.43.350

Shiro, Ohnoki, Hirotoshi, Shibata

{"title":"EVALUATION OF PLATELET SHAPE BY A STOP AND FLOW METHOD USING AGGREGOMETER : Application to Quality Control of Stored Platelet Concentrates","authors":"Shiro, Ohnoki, Hirotoshi, Shibata","doi":"10.3925/jjtc1958.43.350","DOIUrl":"https://doi.org/10.3925/jjtc1958.43.350","url":null,"abstract":"EVALUATION OF PLATELET SHAPE BY A STOP AND FLOW METHOD USING AGGREGOMETER: Application to Quality Control of Stored Platelet Concentrates Shiro Ohnoki and Hirotoshi Shibata Department of Research, Osaka Red Cross Blood Center Platelets change from a discoid to a spherical shape during storage or on activation. Discoid platelets show higher transmittance in the flow state than at rest, but spherical platelets do not. We developed a method for evaluation of platelet shape from the transmittance of PRP using this property. A switch for disconnecting the electrical signal to the recorder and speed controller was attached to the platelet aggregometer, such that it was able to determine the transmittance of PRP at desired stirring rates. Transmittance showed maximum increase at the speed of 800 rpm. The content of spherical platelets was expressed by the extinction ratio (E8/E0) of PRP based on light-scattering theory. Extinction was linearly proportional to the concentration of platelets below 3 X 108, /ml. E8/E0 values correlated with the percentage of spherical platelets but were independent of platelet count and extinction of PRP. Fresh PC had an E8/E0 value of 0. 81 ± 0. 02, with this value of increasing gradually with storage time at 22°C with agitation. These findings demonstrated that it is possible to estimate the shape of platelets with a standard aggregometer by determining the transmittance of PRP at rest and flow.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70325655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ANAPHYLACTIC REACTIONS FOLLOWING TRANSFUSION OF RC-MAP TO A SELECTIVE IGA DEFICIENT PATIENT WITH ANTI-IGA -EFFECTS OF WASHING 选择性iga缺乏患者输血rc-map后的过敏反应与洗涤的抗iga作用

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.46.317

M. Kurosawa, K. Shimano, K. Ikeda, H. Nagahashi, H. Takano, N. Kojima, N. Ishii, H. Gomi, Y. Miwa, M. Negishi, H. Ide, S. Mitsunaga, K. Tadokoro, T. Juji

引用次数: 0

EVALUATION OF HN-2 ANTIBODY INFECTION IN JAPANESE HEMOPHILIACS USING A SYNTHETIC PEPTIDE IMMUNOASSAY AND POLYMERASE CHAIN REATION 应用合成肽免疫分析法和聚合酶链反应评价日本血友病患者hn-2抗体感染

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.36.574

H. Fukui, S. Nishida, Y. Fujimura, H. Shima, A. Yoshioka

{"title":"EVALUATION OF HN-2 ANTIBODY INFECTION IN JAPANESE HEMOPHILIACS USING A SYNTHETIC PEPTIDE IMMUNOASSAY AND POLYMERASE CHAIN REATION","authors":"H. Fukui, S. Nishida, Y. Fujimura, H. Shima, A. Yoshioka","doi":"10.3925/jjtc1958.36.574","DOIUrl":"https://doi.org/10.3925/jjtc1958.36.574","url":null,"abstract":"EVALUATION OF HN-2 ANTIBODY INFECTION IN JAPANESE HEMOPHILIACS USING A SYNTHETIC PEPTIDE IMMUNOASSAY AND POLYMERASE CHAIN REATION Hiromu Fukui, Sachiyo Nishida and Yoshihiro Fujimura Department of Blood Transfusion, Nara Medical College Mikio Kaneda, Hiroko Shima and Akira Yoshioka Department of Pediatrics, Nara Medical College Namiko Yoshihara and Kazue Nakanaga National Institute of Health The antibodies against HIV-2, the alternative etiologic agent for acquired immunodeficiency syndrome (AIDS), were evaluated on a total of 121 Japanese hemophiliacs (106 hemophilia A, and 15 hemophilia B), using a combination of enzyme-immunoassay (ELAVIA-2), western blotting (LABBLOT 2), and peptide immunoassay (PEPTILABI-2) provided from Pasteur-Sanofi, France. The twenty eight patients of hemophilia A (26%) and the three of hemophilia B (20%) showed positive results by ELAVIA-2. PEPTILAB 1-2 test specified that only one patient (case X) with hemophilia A has a double reaction to HIV-1 and HIV-2 specific peptides. LABBLOT 2 also demonstrated the immunoreactivity of case X to HIV-2 proteins; p26, gp36, gp105, and gp140. Case X was further examined using polymerase chain reaction (PCR) method to detect proviral DNA sequences of HIV-1 and HIV-2, and finally the possibility of HIV-2 infection was ruled out confirming true HIV-1 infection.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70309616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0