Nucleic acids research. Supplement (2001)最新文献_第4页

A novel nucleic acid analogue, 3'-amino-2'-deoxy-3',4'-BNA, with an N3'-->P5' phosphoramidate linkage and an S-type conformation. 一种新型核酸类似物，3'-氨基-2'-脱氧-3'，4'-BNA，具有N3'- >P5'磷酸化连锁和s型构象。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.87

Osamu Nakagawa, Akiko Hiroto, Satoshi Obika, Takeshi Imanishi

引用次数: 0

The effect of acridine moiety for binding and reaction of Cu(II)[1 ,4,7-triazacyclononane] with DNA. 吖啶部分对Cu(II)[1,4,7 -三氮杂环壬烷]与DNA结合及反应的影响。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.91

Tomoya Hirohama, Makoto Chikira

引用次数: 0

Deoxyribozymes that catalyze photochemistry: cofactor-dependent and -independent photorepair of thymine dimers. 催化光化学的脱氧核酶:胸腺嘧啶二聚体的辅助因子依赖性和非依赖性光修复。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.217

Dipankar Sen, Daniel J F Chinnapen

{"title":"Deoxyribozymes that catalyze photochemistry: cofactor-dependent and -independent photorepair of thymine dimers.","authors":"Dipankar Sen, Daniel J F Chinnapen","doi":"10.1093/nass/3.1.217","DOIUrl":"https://doi.org/10.1093/nass/3.1.217","url":null,"abstract":"<p><p>Experimental strategies involving in vitro selection, designed to test the validity of the \"RNA World Hypothesis\", have demonstrated a significantly broader catalytic range for RNA (and, nucleic acids in general) than found in naturally occurring ribozymes. We wished to explore whether photochemical reactions could be catalyzed by nucleic acid enzymes. In vitro selection experiments were carried out to obtain \"photolyase\" deoxyribozymes, capable of photoreversing thymine cyclobutane dimers in the presence of a cofactor, serotonin. During in vitro selection from a thymine-dimer containing random DNA library, irradiated with light >300 nm, two pools of catalytic nucleic molecules emerged--one that required serotonin for activity, and another pool that, surprisingly, did not. Characterization of the serotonin-independent clones indicated the optimal wavelength for its repair activity (approximately 1,400-fold) to be approximately 300 nm, notably red-shifted from the absorption maximum of the DNA itself. The folded enzyme may contain a G-quadruplex (whose spectra have red-shifted tails relative to duplex absorbance), and our hypothesis has the folded enzyme as an antenna for the efficient channelling of light or electrons to the thymine dimer, much in the manner of protein photolyases.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"217-8"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40824938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Transition state analogues for enzymes of nucleic acid metabolism. 核酸代谢酶的过渡态类似物。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.107

Vern L Schramm

引用次数: 9

DNA aptamers that recognize fluorophore using on-chip screening in combination with an in silico evolution. DNA适体识别荧光团使用片上筛选与硅进化相结合。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.321

Ryoichi Asai, Shin I Nishimura, Katsutoshi Takahashi

引用次数: 5

Two components model for the biological clock and its evaluation by computer. 生物钟双组分模型及其计算机评价。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.329

Kazuo Yoshida

引用次数: 0

Identification of functional genes by libraries of ribozymes and siRNAs. 利用核酶和sirna文库鉴定功能基因。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.331

Hiroaki Kawasaki, Tomoko Kuwabara, Makoto Miyagishi, Kazunari Taira

引用次数: 4

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab initio calculations. 通过从头计算揭示了稳定3-硝基吡咯与天然核碱基堆积相互作用的重要因素。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.49

Kohji Seio, Hisashi Ukawa, Koh-ichiro Shohda, Mitsuo Sekine

引用次数: 0

Thymidine-based gelators: design, synthesis, and evaluation. 胸腺嘧啶基凝胶:设计、合成和评价。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.27

Sun Min Park, Young Ji Yun, Byeang Hyean Kim

引用次数: 0

Syntheses and structural studies of calix[4]arene-nucleoside and calix[4]arene-oligonucleotide hybrids. 杯[4]芳烃-核苷和杯[4]芳烃-寡核苷酸杂合体的合成与结构研究。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01

Su Jeong Kim, Eun-Kyoung Bang, Byeang Hyean Kim

引用次数: 0