DNA aptamers that recognize fluorophore using on-chip screening in combination with an in silico evolution.

Abstract

We successfully developed a novel screening method for the acquisition of DNA aptamers. The technique selectively recognizes resorufin using on-chip screening in combination with an in silico evolution method. This method proved efficient for screening for DNA aptamers of single-stranded oligo-DNAs. A genetic algorithm was applied to make oligonucleotide sequences for the combinatorial library. A fluorophore, resorufin was applied to the ligand screening as a target. The affinity of the library was analyzed by the DNA microarray. This method for screening DNA ligands includes on-chip selection and a computer-evolved sequence, where the highest affinity was chosen. The fluorescence intensity of the library on the DNA microarray increased after three repetitions of the selection round.