Nucleic acids research. Supplement (2001)最新文献

Molecular recognition in P2 nucleotide receptors. P2核苷酸受体的分子识别。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.3

Kenneth A Jacobson

引用次数: 0

Reaction of nucleosides with 2-acetoxyethyl acetoxymethyl ether. 核苷与2-乙酰氧基乙基乙酰氧基甲基醚的反应。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.11

G Framski, A Manikowski, T Zandecki, J Boryski

引用次数: 1

Synthetic study of cytosaminomycins using the intramolecular glycosylation as a key step. 以分子内糖基化为关键步骤合成胞胺霉素的研究。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.21

Hideyuki Sugimura

引用次数: 4

Development for an efficient synthesis method of 2'-deoxyguanosine-C8 adducts with several amino/nitro-arenes. 氨基/硝基芳烃合成2′-脱氧鸟苷- c8加合物的高效方法。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.23

Takeji Takamura-Enya, Satoko Ishikawa, Masataka Mochizuki, Keiji Wakabayashi

引用次数: 2

Biochemical detection of adenosine and cytidine ionization within RNA by interference analysis. 干扰法检测RNA中腺苷和胞苷的电离。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.229

Scott A Strobel, Fatima D Jones, Adegboyega K Oyelere, Sean P Ryder

{"title":"Biochemical detection of adenosine and cytidine ionization within RNA by interference analysis.","authors":"Scott A Strobel, Fatima D Jones, Adegboyega K Oyelere, Sean P Ryder","doi":"10.1093/nass/3.1.229","DOIUrl":"https://doi.org/10.1093/nass/3.1.229","url":null,"abstract":"Perturbation of active site functional group pKas is an important strategy employed by protein enzymes to achieve catalysis. There is increasing evidence to indicate that RNAs also utilize functional group pKa perturbation for folding and reactivity. The two best candidates for a functionally relevant pKa perturbation are the N3 of C (pKa 4.2) and the N1 of A (pKa 3.5), either of which could be sufficiently raised to allow protonation near physiological pH. Here we report the synthesis and use of a series of alpha-phosphorothioate tagged cytidine and adenosine analogs whose altered pKas make it possible to efficiently detect functionally relevant protonation events by Nucleotide Analog Interference Mapping. This approach has been used to detect ionization events in several catalytic RNAs, including the group I intron, the Hepatitis Delta Virus (HDV), the hairpin and the Varkud Satellite (VS) ribozymes. The active site residue of the VS ribozyme appears to be ionized in the course of the reaction pathway, which may be indicative of a general acid or base mechanism for catalysis by this RNA.","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"229-30"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40824276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Destruction of quadruplex by proteins, and its biological implications in replication and telomere maintenance. 蛋白质对四重体的破坏及其在复制和端粒维持中的生物学意义。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.231

Yoshiaki Enokizono, Akimasa Matsugami, Seiichi Uesugi, Hirokazu Fukuda, Naoto Tsuchiya, Takashi Sugimura, Minako Nagao, Hitoshi Nakagama, Masato Katahira

{"title":"Destruction of quadruplex by proteins, and its biological implications in replication and telomere maintenance.","authors":"Yoshiaki Enokizono, Akimasa Matsugami, Seiichi Uesugi, Hirokazu Fukuda, Naoto Tsuchiya, Takashi Sugimura, Minako Nagao, Hitoshi Nakagama, Masato Katahira","doi":"10.1093/nass/3.1.231","DOIUrl":"https://doi.org/10.1093/nass/3.1.231","url":null,"abstract":"The minisatellite DNA Pc-1 consists of tandem repeats of d(GGCAG). We previously reported that a d(GGCAG)n strand folds into an intramolecular quadruplex under physiological conditions and that during replication the progression of DNA polymerase is blocked by the quadruplex in vitro. Therefore, the formation of the quadruplex was supposed to be responsible for the hypermutable features of Pc-1. Then, we have identified proteins that bind to Pc-1, one of which is hnRNP A1. Here, we have demonstrated that hnRNP A1 destroys the quadruplex of Pc-1 on binding and abrogates the arrest of DNA polymerase at the repeat. Thus, hnRNP A1 functions as if it is a chaperon to assist Pc-1 DNA to form the proper folding suitable for replication. We have also found that hnRNP A1 and a related protein, hnRNP D, destroy the quadruplex of telomere DNA, which suggests the involvement of these proteins in telomere maintenance as DNA chaperons.","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"231-2"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40824277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Site-selective DNA hydrolysis by the combination of Ce(IV) and oligonucleotide bearing EDTA groups. Ce(IV)和EDTA寡核苷酸组合的位点选择性DNA水解。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.137

Hiroyuki Arishima, Masahito Yokoyama, Makoto Komiyama

引用次数: 1

2,6-Diaminonaphthyridine derivatives bind to a single nucleotide bulge in DNA. 2,6-二氨基萘啶衍生物与DNA中的单个核苷酸凸起结合。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.139

Hitoshi Suda, Shinya Hagihara, Akio Kobori, Kazuhiko Nakatani, Isao Saito

引用次数: 0

Study on interaction of a cationic porphyrin with DNA. 阳离子卟啉与DNA相互作用的研究。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.189

Takako Ohyama, Aya Sasagawa, Norifumi Terui, Hajime Mita, Yasuhiko Yamamoto

引用次数: 0

Development of a probe DNA which accompanies color change on hybridization. 杂交过程中伴随颜色变化的探针DNA的研制。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI: 10.1093/nass/3.1.143

Hiromu Kashida, Hiroyuki Asanuma, Makoto Komiyama

引用次数: 0