{"title":"2,6-二氨基萘啶衍生物与DNA中的单个核苷酸凸起结合。","authors":"Hitoshi Suda, Shinya Hagihara, Akio Kobori, Kazuhiko Nakatani, Isao Saito","doi":"10.1093/nass/3.1.139","DOIUrl":null,"url":null,"abstract":"<p><p>2,6-Diamino-1,8-naphthyridine derivative (daNpt), which possesses hydrogen bonding groups in an alignment of donor-acceptor-acceptor-donor binds to a single nucleotide bulge in the duplex. The melting temperatures (Tm) of all duplexes containing a bulge were increased, especially for the cytosine (C) and thymine (T) bulges, in the presence of daNpt, whereas only a small increase of Tm was observed for the fully matched duplexes. It was suggested by pH dependency of Tm and UV spectra, that a protonation of daNpt should play an important role for the recognition of C and T bulges.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"139-40"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.139","citationCount":"0","resultStr":"{\"title\":\"2,6-Diaminonaphthyridine derivatives bind to a single nucleotide bulge in DNA.\",\"authors\":\"Hitoshi Suda, Shinya Hagihara, Akio Kobori, Kazuhiko Nakatani, Isao Saito\",\"doi\":\"10.1093/nass/3.1.139\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>2,6-Diamino-1,8-naphthyridine derivative (daNpt), which possesses hydrogen bonding groups in an alignment of donor-acceptor-acceptor-donor binds to a single nucleotide bulge in the duplex. The melting temperatures (Tm) of all duplexes containing a bulge were increased, especially for the cytosine (C) and thymine (T) bulges, in the presence of daNpt, whereas only a small increase of Tm was observed for the fully matched duplexes. It was suggested by pH dependency of Tm and UV spectra, that a protonation of daNpt should play an important role for the recognition of C and T bulges.</p>\",\"PeriodicalId\":86149,\"journal\":{\"name\":\"Nucleic acids research. Supplement (2001)\",\"volume\":\" 3\",\"pages\":\"139-40\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/nass/3.1.139\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nucleic acids research. Supplement (2001)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/nass/3.1.139\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.139","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
2,6-Diaminonaphthyridine derivatives bind to a single nucleotide bulge in DNA.
2,6-Diamino-1,8-naphthyridine derivative (daNpt), which possesses hydrogen bonding groups in an alignment of donor-acceptor-acceptor-donor binds to a single nucleotide bulge in the duplex. The melting temperatures (Tm) of all duplexes containing a bulge were increased, especially for the cytosine (C) and thymine (T) bulges, in the presence of daNpt, whereas only a small increase of Tm was observed for the fully matched duplexes. It was suggested by pH dependency of Tm and UV spectra, that a protonation of daNpt should play an important role for the recognition of C and T bulges.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
