{"title":"杂交过程中伴随颜色变化的探针DNA的研制。","authors":"Hiromu Kashida, Hiroyuki Asanuma, Makoto Komiyama","doi":"10.1093/nass/3.1.143","DOIUrl":null,"url":null,"abstract":"<p><p>Naphthyl Red moiety, conjugated to DNA, shows distinct chromism by hybridization with its complementary DNA. Single-stranded DNA involving Naphthyl Red moiety exhibits orange color and has lambda max at 466 nm at pH 7.0. Absorption maximum shifts towards 545 nm by the presence of its complementary DNA, and accordingly color of the solution changes from orange to magenta.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"143-4"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.143","citationCount":"0","resultStr":"{\"title\":\"Development of a probe DNA which accompanies color change on hybridization.\",\"authors\":\"Hiromu Kashida, Hiroyuki Asanuma, Makoto Komiyama\",\"doi\":\"10.1093/nass/3.1.143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Naphthyl Red moiety, conjugated to DNA, shows distinct chromism by hybridization with its complementary DNA. Single-stranded DNA involving Naphthyl Red moiety exhibits orange color and has lambda max at 466 nm at pH 7.0. Absorption maximum shifts towards 545 nm by the presence of its complementary DNA, and accordingly color of the solution changes from orange to magenta.</p>\",\"PeriodicalId\":86149,\"journal\":{\"name\":\"Nucleic acids research. Supplement (2001)\",\"volume\":\" 3\",\"pages\":\"143-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/nass/3.1.143\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nucleic acids research. Supplement (2001)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/nass/3.1.143\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.143","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development of a probe DNA which accompanies color change on hybridization.
Naphthyl Red moiety, conjugated to DNA, shows distinct chromism by hybridization with its complementary DNA. Single-stranded DNA involving Naphthyl Red moiety exhibits orange color and has lambda max at 466 nm at pH 7.0. Absorption maximum shifts towards 545 nm by the presence of its complementary DNA, and accordingly color of the solution changes from orange to magenta.
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