Asian Pacific Journal of Reproduction最新文献

{"title":"Impact of chronological ageing on semen parameters in southern Indian men visiting infertility centre: A retrospective study","authors":"M. Asif, A. Vijay, Maheshwari, Syed Fyzullah, Usha Rani, R. Swathi, K. D. Damodara Gowda","doi":"10.4103/2305-0500.365227","DOIUrl":"https://doi.org/10.4103/2305-0500.365227","url":null,"abstract":"Objective: To investigate the association between age and semen parameters among male partners of subfertile couples. Methods: This retrospective study analyzed the semen of 1 523 infertile men aged 26 to 50 years. Data were extracted from GarbhaGudi IVF Centre database from January 2019 to September 2020. The basic semen parameters were interpreted according to the WHO manual 2021, 6th edition. Semen parameters in different age groups were compared. Results: Total and progressive motile sperms were significantly higher in the age group of 26-30 years compared to other age groups (P<0.05). Normal sperm count was significantly higher in the age group of 26-30 years compared to the age groups of 41-45 years and >46 years (P=0.001). However, sperm head defects, neck and midpiece defects, tail defects, and cytoplasmic droplets showed statistically insignificant difference in all the age groups (P>0.05). Semen viscosity showed no statistical difference in all the age groups compared to the reference age group of 26 to 30 years. Conclusions: Higher age can lead to a significant decrease in normal sperms and motility in subfertile men. Hence, male partner age should be considered as one of the major determining factors for reproductive outcomes.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"10 - 15"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43697025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of N-acetylcysteine on growth, viability and reactive oxygen species levels in small antral follicles cultured in vitro","authors":"Efigênia Cordeiro, B. Silva, L. Paulino, P. Barroso, L. Barrozo, Miguel F De Lima Neto, J. Silva","doi":"10.4103/2305-0500.365231","DOIUrl":"https://doi.org/10.4103/2305-0500.365231","url":null,"abstract":"Objective: To investigate the effects of different concentrations of N-acetylcysteine on follicular growth and morphology, as well as on viability, levels of reactive oxygen species (ROS) and meiotic progression of oocytes from in vitro cultured bovine early antral follicles. Methods: Isolated early antral follicles (about 500 μm) were cultured in TCM-199+ alone or supplemented with 1.0, 5.0 or 25.0 mM N-acetylcysteine at 38.5 °C with 5% CO2 for 8 days. Follicle diameters were evaluated at day 0, 4 and 8 of culture. At the end of culture, the levels of ROS, chromatin configuration and viability (calcein-AM and ethidium homodimer-1 staining) were investigated in the cumulus-oocyte complexes. Comparisons of follicle diameters between treatments were performed. Data on percentages of morphologically normal follicles, growth rates and chromatin configuration in different treatments were compared. Results: An increase in follicular diameters after culture in all treatments was observed, except for follicles cultured with 25.0 mM N-acetylcysteine. Fluorescence microscopy showed that oocytes cultured in all treatments were stained positively with calcein-AM, and that 5.0 mM N-acetylcysteine reduced fluorescence for ethidium homodimer-1. Intracellular levels of ROS in oocytes from follicles cultured with 1.0 mM N-acetylcysteine showed a significant reduction compared to other treatments. The presence of N-acetylcysteine in culture medium did not influence the rates of oocyte at the germinal vesicle stage. Conclusions: N-acetylcysteine at concentrations of 1.0 and 5.0 mM reduces ROS levels and staining for ethidium homodimer-1 in in vitro cultured follicles, respectively, while 25.0 mM N-acetylcysteine decreases follicular growth and the percentages of continuously growing follicles.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"42 - 48"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46622897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel herbal combination ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with letrozole-induced polycystic ovary syndrome","authors":"S. Shailajan, S. Menon, Swati Singh, Yugandhara M Patil","doi":"10.4103/2305-0500.365229","DOIUrl":"https://doi.org/10.4103/2305-0500.365229","url":null,"abstract":"Objective: To investigate the potential activity of novel herbal combination and novel herbal formulation (syrup) in female Sprague Dawley rats with letrozole-induced polycystic ovary syndrome (PCOS). Methods: Forty-two rats were randomly divided into seven groups with six rats in each group. Group 1 received 0.5% carboxy methylcellulose orally for 37 days and served as the normal control group. Group 2 was orally administered with letrozole of 1 mg/kg for 21 days and served as the PCOS induction group without treatment. Group 3 to 7 were administered with letrozole of 1 mg/kg for 21 days orally to induce PCOS, and then respectively received clomiphene citrate at 1 mg/kg, 100 and 200 mg/kg of novel herbal combination, 200 mg/kg of novel herbal formulation (syrup), and 400 mg/kg of marketed formulation of Pushyanuga churna, once daily for 15 days. Effects of the novel herbal combination and its syrup formulation were evaluated on the hormonal profile, the levels of antioxidants, the lipid profile and on the ovarian morphology, using letrozole-induced PCOS model in rats. Results: Letrozole caused alterations in hormonal levels and lipid levels similar to PCOS and ovarian histology showed presence of ovarian cysts confirming the induction of PCOS in rats. On treatment with the novel herbal combination and its syrup formulation in PCOS-induced rats, the altered hormonal and lipid profiles showed significant recovery to normal levels. Ovarian histology confirmed the restoration of folliculogenesis in the PCOS-induced rats. The treatment with the syrup formulation of novel herbal combination was found to be more effective than novel herbal combination and showed better recovery in various parameters evaluated. The results of the study, however, suggested that treatment with novel herbal combination and its syrup formulation provided minimal protection against oxidative stress caused due to the induction of PCOS. Conclusions: The integrated approach for management of PCOS is to counterbalance the limitations associated with modern therapy. Both the novel herbal combination and the syrup formulation of novel herbal combination show efficacy in the management of PCOS in rats and restore folliculogenesis in the ovary. The syrup formulation of novel herbal combination is most effective in the management of PCOS and shows potential to be developed as an adjuvant therapeutic agent.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"23 - 34"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41734110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The fate of surplus embryos in the setting of assisted reproductive technology: A scoping review","authors":"I. Agung Sentosa, S. Ayuandari, Rafhani Rosyidah, Agung Dewanto","doi":"10.4103/2305-0500.365226","DOIUrl":"https://doi.org/10.4103/2305-0500.365226","url":null,"abstract":"Objective: To identify the attitudes of infertile couples toward their surplus frozen embryos. Methods: This study was according to PRISMA-ScR as the guideline for scoping review. Studies that assessed the attitudes of patients or infertile couples who had surplus embryos were included. We conducted systematic searches in English studies from April 2011-April 2021 using 7 databases: PubMed, Science Direct, EBSCO, Scopus, the Cochrane Library, Sage Journals, and Google Scholar. Data were charted based on author, year of publication, country, purpose, data collection, key findings, and research focus/domain. Results: A total of 37 research articles were included in the analysis. Their attitudes encompassed: supporting the donation of the surplus embryos for both research and reproductive purposes, continuing to store the frozen embryos, and disposing of the surplus embryos. Conclusions: Most of the infertile patients support donating their surplus embryos for research and reproductive purposes.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"1 - 9"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43149951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum anti-leukemia inhibitory factor antibody and recurrent pregnancy loss in Iranian women","authors":"S. Seifati, Hossein Ansariniya, Fateme Rahmani, A. Saboor-Yaraghi, F. Fesahat, H. Hadinedoushan, F. Zare","doi":"10.4103/2305-0500.365232","DOIUrl":"https://doi.org/10.4103/2305-0500.365232","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"49 - 50"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44355345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriospermia among smallholder artificial insemination boars in the Philippines and potential associated factors","authors":"Santiago T. Peña Jr., M. Pagente, Bianca Ymas, Mark Janier","doi":"10.4103/2305-0500.365230","DOIUrl":"https://doi.org/10.4103/2305-0500.365230","url":null,"abstract":"Objective: To determine the prevalence of bacteriospermia, the bacterial load, and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination operators. Methods: Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte, Philippines and were subjected to standard bacteriological culture and identification, including a survey of potentially associated factors. Prevalence and bacterial count were determined accordingly, while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering technique. Results: One hundred percent contamination with a bacterial count of (2.01±0.38)×103 CFU/mL was observed. At least 73.33% of the samples were positive for Bacillus spp., while other identified isolates included Enterobacter spp., Staphylococcus spp., E. coli, Pseudomonas spp., Citrobacter spp., and Klebsiella spp. Conclusions: Despite the high prevalence of bacteriospermia, the bacterial count is low. Nevertheless, on-farm practices on boar health and management, semen collection, and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"35 - 41"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48159872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive outcomes of water pipe smoking: A scoping review","authors":"Fardin Amidi, Sahar Rostami, Farzane Fereidouni, Arezoo Maleki-Hajiagha, Mohadese Motaharinejad, Somayye Majidi","doi":"10.4103/2305-0500.386122","DOIUrl":"https://doi.org/10.4103/2305-0500.386122","url":null,"abstract":"Objective: To present an overview of the adverse effects of water pipe smoking on various aspects of reproduction and perinatal complications, covering all the most relevant studies, from descriptive findings to clinical trials. Methods: We reviewed the literature and included full publications in English provided by international biomedical databases, including PubMed, Science Direct, EBSCO, Scopus, and Google Scholar, and Cochrane Library over the last 38 years (May 1985 to June 2023). Search terms (Water pipe, Narghile, Shisha, Dokha, etc., and Reproduction, Fertility, etc.) were adopted from Medical Subheading (MeSH) and Boolean operators were utilized to improve sensitivity. Results: 133 Papers were identified in the initial search, of which 29 were excluded due to duplication. Eliminating search overlap and irrelevant records by reviewing titles and abstracts reduced 42 papers. 62 Full-publication articles met the inclusion criteria. We summarized the effects of waterpipe smoke exposure on reproductive health based on three main categories: male and female fertility, fetal and neonatal outcomes, and pregnancy complications. Conclusions: Water pipe smoking has potential adverse effects on different aspects of human reproductive health, including semen parameters, male and female fertility, and fetal and neonatal health, and contributes to many maternal complications and morbidities.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135009130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of small non-coding RNAs (sncRNAs) in male infertility: A scoping review","authors":"Hacer Kaya Cakir, Onur Eroglu","doi":"10.4103/2305-0500.386121","DOIUrl":"https://doi.org/10.4103/2305-0500.386121","url":null,"abstract":"Objective: To give a brief overview of the field of epigenetics and the potential predictive power that small non-coding RNA (sncRNA) may hold in relation to improving the treatment and diagnosis of male infertility. Methods: PRISMA-ScR was used as the scoping review guideline for this investigation. All article data here have been accessed from MEDLINE–PubMed, Science Direct, EBSCO, Scopus, Sage Journals, and Google Scholar. The terms \"small non coding RNA, male, infertility, miRNA, sperm\" were used in the search between 2015 and 2023. Results: The study comprised 35 publications in total. Several sncRNAs, miR-155, miR-16, miR-196, miR-525-3p, miR-891 were found to be effective in regulating the mechanism of spermatozoa processing in the infertility of men. sncRNA can be used as a biomarker of male infertility. Conclusions: sncRNAs can act as biomarkers for the diagnosis of reproductive diseases. Actually, by recognizing sncRNAs and their mechanisms, a new way to treat infertile men would be paved. The functional annotation of sncRNAs in spermatogenesis is still in its infancy but has enormous potential. This is despite the fact that many potential sncRNAs have been found to date with the use of cutting-edge technology and publicly accessible sncRNA annotation tools.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135010277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of taxifolin on cold-shock damages in spermatozoa in rabbits","authors":"İbrahimHalil Güngör, İrfan Yılmaz, Gaffari Türk, AslıhanÇakır Cihangiroğlu, TutkuCan Acısu, Gözde Arkalı, EdanurGüler Ekmen","doi":"10.4103/2305-0500.386125","DOIUrl":"https://doi.org/10.4103/2305-0500.386125","url":null,"abstract":"Objective: To investigate the effect of taxifolin added to rabbit semen on freezing-induced cold-shock damages in spermatozoa. Methods: Semen was collected from six adult New Zealand rabbits once a week by artificial vagina. The collected semen was pooled at 38 °C and divided into four equal volumes. They were diluted with 0, 50, 100 and 200 μM taxifolin-containing Tris + egg yolk extender at 38 °C and their temperatures were lowered to 4 °C. Following equilibration, semen drawn into 0.25 mL straws were frozen in an automatic semen freezing device and stored in liquid nitrogen container at -196 °C. Samples were thawed in 38 °C water for 25 s and the analyses of motility, kinematic parameters, morphological deformities, changes in membrane integrity, mitochondrial membrane potential, dead-live ratio, acrosomal damages and as well as oxidative stress analyses were performed in semen. Results: Addition of 50 μM taxifolin significantly improved motility (total, progressive, rapid and static), high mitochondrial membrane potential and the ratios of spermatozoa with acrosomal damage compared to the control group. Compared to the control group, malondialdehyde (MDA) levels in the 50 and 100 μM taxifolin groups were significantly lower, while the MDA level was high and viable spermatozoa ratio was low in the 200 μM taxifolin group. There were no significant differences between the groups in terms of kinematic parameters, morphological deformities, membrane integrity and antioxidant levels. Conclusions: The low dose of taxifolin (50 μM) has a positive effect and the high dose (200 μM) has a negative effect. Therefore, it is concluded that the addition of low-dose (50 μM) taxifolin to the extenders would be a useful additive in reducing cold-shock damage that occurs during freezing of rabbit semen.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135008590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanolic pomegranate dried peel extract improves cryopreserved semen quality and antioxidant capacity of rams","authors":"AmalM Aboelmaaty, MohamedS Kotp, AyaM Fadl, ElshymaaA Abdelnaby, IslamE El-Seadawy, HossamR El-Sherbiny","doi":"10.4103/2305-0500.386124","DOIUrl":"https://doi.org/10.4103/2305-0500.386124","url":null,"abstract":"Objective: To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams' semen extender for obtaining the best-cryopreserved semen quality. Methods: Tris-based semen extender was supplemented with 0.0, 0.40, 0.48, and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months (n=80). Pooled (n=16) post-thaw semen characteristics were determined. Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GPx), superoxide dismutase (SOD), ascorbic acid, zinc, copper, total cholesterol, low-density lipoproteins (LDL), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP). Results: The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility (P<0.001), sperm progressive motility (P<0.001), live sperm (P<0.001), sperm plasma membrane integrity (P<0.001), acrosome integrity (P<0.001), SOD (P<0.05), zinc (P<0.001), total cholesterol (P<0.001), and LDL (P<0.001) with the lowest percentage of abnormal sperm morphology (P<0.001), the lowest lipid peroxidation (MDA, P<0.01), ascorbic acid (P>0.05), and LDH (P>0.05). Conclusions: Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135009117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}