Asian Pacific Journal of Reproduction最新文献

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Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial 肌醇与二甲双胍对多囊卵巢综合征不孕妇女临床特征、内分泌和代谢特征的影响:随机对照试验
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390300
N.S.V. Le, Minh Tam Le, T. N. Cao
{"title":"Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial","authors":"N.S.V. Le, Minh Tam Le, T. N. Cao","doi":"10.4103/2305-0500.390300","DOIUrl":"https://doi.org/10.4103/2305-0500.390300","url":null,"abstract":"\u0000 \u0000 To compare the effectiveness of inositol and metformin on the clinical characteristics, and endocrine and metabolic profiles of infertile polycystic ovarian syndrome (PCOS) women from Vietnam.\u0000 \u0000 \u0000 \u0000 From June 2018 to August 2022, a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome. The clinical, endocrine, and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day. Natural pregnancy rates, adverse effects, and tolerance of inositol were recorded.\u0000 \u0000 \u0000 \u0000 The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment, of whom 132 women participated in data analysis after 3 months. After metformin treatment, 42.1% of women with oligomenorrhea experienced regular menstruation. Metformin significantly lowered body mass index (BMI), waist circumference and testosterone levels, but had no effect on other clinical characteristics, endocrine profiles, or metabolic profiles. 29.2% Of women reported experiencing side effects. 21% Of them attained pregnancy, which resulted in 17.1% of live births. In the inositol group, the rate of regular cycle increased by 18.2% and the total testosterone concentration significantly decreased. In overweight/obese women with PCOS, inositol significantly decreased weight, BMI, waist and hip circumferences (P<0.05). 100% Of women tolerated inositol and continued treatment. 18.9% Of them became pregnant, leading to 17% of live births.\u0000 \u0000 \u0000 \u0000 Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS. Metformin is associated with a higher rate of regular menstruation, whereas inositol is associated with a lower rate of adverse effects. The spontaneous conception, clinical pregnancy, and live birth rates between two groups are comparable.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"535 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138993025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study 新鲜卵母细胞与玻璃化卵母细胞的胚胎、遗传和临床结果:回顾性队列研究
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390301
Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh
{"title":"Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study","authors":"Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh","doi":"10.4103/2305-0500.390301","DOIUrl":"https://doi.org/10.4103/2305-0500.390301","url":null,"abstract":"\u0000 \u0000 To compare embryonic development, ploidy status and clinical outcomes between fresh and frozen-thawed oocytes.\u0000 \u0000 \u0000 \u0000 This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in Vietnam. The patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be vitrified. In the last oocyte retrieval, all patient's oocytes including both frozen and fresh would be fertilized. The outcomes included the rates of oocyte survival, cleavage embryo, blastocyst, ploidy status, pregnancy, biochemical pregnancy and clinical pregnancy.\u0000 \u0000 \u0000 \u0000 The oocyte survival rate after thawing was 96.5%. No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate (78.1% vs. 75.5%, P=0.461), usable cleavage embryo rate (86.9% vs. 87.2%, P=0.916) but usable blastocyst rate was found higher statistically in the frozen oocyte group (44.4% vs. 54.0%, P=0.049). The percentages of euploid, aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences (33.8% vs. 31.6%, P=0.682; 51.0% vs. 54.2%, P=0.569; 15.2% vs. 12.4%, P=0.787; respectively). The rates of pregnancy, biochemical pregnancy and clinical pregnancy had no statistical difference (68.8% vs. 64.8%, P=0.764; 12.5% vs. 3.6%, P=0.258; 37.5% vs. 46.4%, P=0.565). 17 Mature oocytes are the minimum to have at least one euploid embryo.\u0000 \u0000 \u0000 \u0000 Oocyte vitrification does not affect embryonic, genetic and clinical results. The number of mature oocytes should be considered for fertilization in some cases.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"345 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro 低温保存的绵羊精原干细胞在体外保持干性和集落形成能力
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390302
R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra
{"title":"Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro","authors":"R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra","doi":"10.4103/2305-0500.390302","DOIUrl":"https://doi.org/10.4103/2305-0500.390302","url":null,"abstract":"\u0000 \u0000 To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells (SSCs) in vitro.\u0000 \u0000 \u0000 \u0000 Sheep testicular cells were isolated and putative SSCs were enriched by the laminin-based differential plating method. Putative SSCs were co-cultured with the Sertoli cell feeder prepared by the Datura Stramonium Agglutinin (DSA-lectin)-based method. The cultured putative SSCs were cryopreserved in Dulbecco's Modified Eagle Medium-10% fetal bovine serum mixture (DMEM-10% FBS) media containing 10% dimethyl sulfoxide (DMSO) alone or 10% DMSO plus 200 mM trehalose. Cryopreserved putative SSCs were evaluated for their proliferation potential using in vitro culture and stemness by immunocytochemistry. Finally, the transfection ability of cryopreserved putative SSCs was analyzed.\u0000 \u0000 \u0000 \u0000 We isolated 91% viable testicular cells from sheep testes. The majority of the laminin enriched cells expressed the SSC related marker, ITGA6. Co-culture of sheep putative SSCs with Sertoli cell feeder resulted in the generation of stable colonies, and the expression of SSC marker was maintained after several passages. A significantly higher number of viable putative SSCs was recovered from SSCs cryopreserved in media containing 10% DMSO and 200 mM trehalose compared to 10% DMSO alone (P<0.01). Cryopreserved putative SSCs formed colonies and showed SSC marker expression similar to the non-cryopreserved putative SSCs. The appearance of green fluorescent colonies over the Sertoli cell feeder indicated that cryopreserved sheep SSCs were successfully transfected.\u0000 \u0000 \u0000 \u0000 Cryopreserved putative SSCs can retain their stemness, colony forming ability, and transfection efficiency in vitro. Our research may help in the effective preservation of germplasm and the generation of transgenic ovine species.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"572 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands 季节应激对安达曼和尼科巴群岛濒危泰瑞莎山羊精液质量、精液生化和氧化应激属性的影响
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390304
P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar
{"title":"Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands","authors":"P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar","doi":"10.4103/2305-0500.390304","DOIUrl":"https://doi.org/10.4103/2305-0500.390304","url":null,"abstract":"\u0000 \u0000 To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05).\u0000 \u0000 \u0000 \u0000 Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"199 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of VEGF165 and VEGF165b during ovarian follicular development 卵巢卵泡发育过程中 VEGF165 和 VEGF165b 的表达
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390303
Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong
{"title":"Expression of VEGF165 and VEGF165b during ovarian follicular development","authors":"Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong","doi":"10.4103/2305-0500.390303","DOIUrl":"https://doi.org/10.4103/2305-0500.390303","url":null,"abstract":"\u0000 \u0000 To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles.\u0000 \u0000 \u0000 \u0000 We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence.\u0000 \u0000 \u0000 \u0000 The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h.\u0000 \u0000 \u0000 \u0000 This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"113 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidative stress in male infertility and therapeutic approach: A mini-review 男性不育症中的氧化应激与治疗方法:微型综述
Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI: 10.4103/2305-0500.390299
Marwa Lahimer, H. Mustapha, Véronique Bach, H. Khorsi-Cauet, Moncef Benkhalifa, Mounir Ajina, Habib Ben Ali
{"title":"Oxidative stress in male infertility and therapeutic approach: A mini-review","authors":"Marwa Lahimer, H. Mustapha, Véronique Bach, H. Khorsi-Cauet, Moncef Benkhalifa, Mounir Ajina, Habib Ben Ali","doi":"10.4103/2305-0500.390299","DOIUrl":"https://doi.org/10.4103/2305-0500.390299","url":null,"abstract":"Growing evidence suggests that oxidative stress is a major cause of male infertility. Spermatozoa are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant defense. Reactive oxygen species (ROS) impact multiple signaling pathways involved in mitochondrial dysfunction, hormonal unbalance and semen quality decline. The origin of ROS is diverse, including generated normal cellular metabolism, environmental exposure, advanced paternal age and inflammation. Research has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic diseases. Moreover, it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility treatments. The current study highlights the deleterious effect of ROS and its impact on semen function. In addition, it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"279 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plantain-based diet modulates atrazine-induced testicular toxicities in rats 以大车前草为基础的饮食调节阿特拉津诱导的大鼠睾丸毒性
Asian Pacific Journal of Reproduction Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380984
D. Rotimi, O. Adeyemi
{"title":"Plantain-based diet modulates atrazine-induced testicular toxicities in rats","authors":"D. Rotimi, O. Adeyemi","doi":"10.4103/2305-0500.380984","DOIUrl":"https://doi.org/10.4103/2305-0500.380984","url":null,"abstract":"Objective: To assess the potential of plantain-based diet in modulating testicular toxicities in rats exposed to atrazine. Methods: The plantain-based diet at 50%, 25% and 12.5% were prepared from the basal diet by substituting the corn starch with plantain fruit pulp flour at different percentages. Wistar rats were fed plantain-based diet in varying concentrations ranging from 12.5% to 50% of the basal diet for 21 days before or after atrazine treatment in a two-phase experiment: preventive and therapeutic phases. The therapeutic model (n=35) had seven groups with 5 rats each, including the control, atrazine, atrazine recovery, atrazine plus plantain-based diet 50%, 25%, 12.5%, and atrazine plus quercetin groups. The preventive model (n=30) had six groups of 5 rats, consisting of the control, atrazine, 50%, 25%, 12.5% plantain-based diet plus atrazine, and quercetin plus atrazine groups. Gonadal hormones (testosterone, luteinizing hormone and follicle-stimulating hormone), sperm parameters (sperm motility, viability, morphology and concentration), and testicular function indices (protein, cholesterol, glycogen, acid phosphatase, alkaline phosphatase and lactate dehydrogenase) were measured. Results: The gonadal hormones, sperm characteristics, and testicular function indices of the rat testis decreased significantly in the atrazine group alongside degeneration of the histoarchitecture. However, plantain-based diet restored the gonadal hormone concentrations, semen parameters, and testicular function indices in both the preventive and therapeutic models. Conclusions: Treatment with plantain-based diet protects against rat testicular toxicity caused by atrazine via the modulation of gonadal hormones, sperm quality, testicular function index as well as histoarchitecture of rat testes.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"170 - 181"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47665593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles: A multicentric prospective cohort study 在拮抗剂新鲜胚胎移植周期中补充单剂量GnRH激动剂以维持黄体期后的妊娠结局:一项多中心前瞻性队列研究
Asian Pacific Journal of Reproduction Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380983
Tatapudi Parvathi Devi, A. Gahlot, Sangita Sharma, M. Choudhary, R. Soni, Meeta Sharma
{"title":"Pregnancy outcomes following supplementation of single dose GnRH agonist to sustain the luteal phase in antagonist fresh embryo transfer cycles: A multicentric prospective cohort study","authors":"Tatapudi Parvathi Devi, A. Gahlot, Sangita Sharma, M. Choudhary, R. Soni, Meeta Sharma","doi":"10.4103/2305-0500.380983","DOIUrl":"https://doi.org/10.4103/2305-0500.380983","url":null,"abstract":"Objective: To determine whether a single dose of gonadotropin-releasing hormone (GnRH) agonist administered subcutaneously in addition to the regular progesterone supplementation could provide a better luteal support in antagonist protocol fresh embryo transfer cycles. Methods: This prospective, multicentric, cohort study included total 140 women, 70 in each group. Controlled ovarian stimulation was carried out as per fixed GnRH antagonist protocol. The trigger was given with hCG. In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) was performed and day-3 embryos were transferred. Patients were divided into groups 1 and 2 based on computer generated randomization sheet. Six days following oocyte retrieval, group 1 received 0.2 mg decapeptyl subcutaneously in addition to regular progesterone support while group 2 received progesterone only. Luteal support was given for 14 days to both groups; if pregnancy was confirmed luteal support was continued till 12 weeks of gestation. The clinical pregnancy rate was the primary outcome. The implantation rate, miscarriage rate, live birth delivery rate, and multiple pregnancy rates were the secondary outcomes. Results: A total of 140 patients were analysed, 70 in each group. Clinical pregnancy rates (47.1% vs. 35.7%; P=0.17), implantation rates (23.4% vs. 18.1%, P=0.24), live birth delivery rates (41.4% vs. 27.1%, P=0.08), and multiple pregnancy rates (21.2% vs. 16.0%, P=0.74) were higher in group 1 than in group 2. Group 1 had a lower miscarriage rate than group 2 (5.7% vs. 8.6%; P=0.75). However, these differences were not statistically significant between the two groups. Conclusions: Administration of a single dose of GnRH agonist in addition to regular natural micronized vaginal progesterone as luteal support in GnRH antagonist protocol cycles marginally improves implantation rates, clinical pregnancy rates, and live birth delivery rates. However, more studies with higher sample sizes are needed before any conclusive statements about GnRH agonist as luteal phase support can be made.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"162 - 169"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48233969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of the ovine ovarian reserve during the prenatal and neonatal periods 产前和新生儿期绵羊卵巢储备的测定
Asian Pacific Journal of Reproduction Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380986
Isam B. Sharum
{"title":"Determination of the ovine ovarian reserve during the prenatal and neonatal periods","authors":"Isam B. Sharum","doi":"10.4103/2305-0500.380986","DOIUrl":"https://doi.org/10.4103/2305-0500.380986","url":null,"abstract":"Objective: To determine the ovine ovarian histomorphology and follicular staging at various age periods in Awassi breed. Methods: Ovaries were collected from prenatal fetuses [gestational age (95±5) days], neonatal (day 0), and prepubertal ewe lambs (two and four months of age); each age group included six animals. Ovaries (n=12, each group) were dissected and processed for hematoxylin and eosin staining. Stained sections (n=24, each group) were imaged and utilized for histomorphology assessment, follicle measurement, and classification. Results: Prenatal ovaries were mainly enriched with primordial follicles accompanied by a lower proportion of primary follicles. In addition to primordial and primary follicles, neonatal ovaries demonstrated a proportion of centrally located multilayered and antral follicles. In comparison with neonatal ovaries, the proportion of multilayered and antral follicles was significantly higher in the ovaries of two-month-old lambs; conversely, the proportion of peripherally situated primordial follicles dramatically declined compared to that of earlier age of lamb. Although there was no statistical variation in the sizes of primordial follicles across groups, the mean diameter of the primary follicle in the prenatal ovaries was substantially smaller than in postnatal ovaries. Compared to the neonatal ovaries, the size of the multilayered and antral follicles in the prepubertal ovaries was substantially larger. Conclusions: The earliest follicular developmental stages were established prenatally whereas the advanced growth stages started in the neonatal period and greatly increased in the prepubertal period.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"194 - 200"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48731045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The barriers of joining in vitro fertilization programs among infertile couples in developing countries: A scoping review 发展中国家不孕夫妇参与体外受精项目的障碍:范围界定综述
Asian Pacific Journal of Reproduction Pub Date : 2023-07-01 DOI: 10.4103/2305-0500.380981
A. Dewi, A. Wicaksana, Muhammad Lutfi, Agung Dewanto
{"title":"The barriers of joining in vitro fertilization programs among infertile couples in developing countries: A scoping review","authors":"A. Dewi, A. Wicaksana, Muhammad Lutfi, Agung Dewanto","doi":"10.4103/2305-0500.380981","DOIUrl":"https://doi.org/10.4103/2305-0500.380981","url":null,"abstract":"Objective: To determine the barriers of joining in-vitro fertilization (IVF) programs among infertile couples in developing countries. Methods: This study assessed infertile couples and the barriers or associated factors resulting in delayed decision-making of joining IVF program by searching databases PubMed, ScienceDirect, EBSCO, ClinicalKey, and Cochrane Library from inception until December 31, 2021. Additional search strategies were snowballing literature search and citation tracking. Results: Eleven articles were included in the scoping review. The cost was the greatest barrier of joining IVF program. Limited access and lack of assisted reproductive technology centers, few qualified infertility trained staff, insufficient government support, low priority in government policy, along with sociocultural factors, such as religion and false beliefs or myths were also majorly considered to be associated obstacles. Conclusions: The main barrier associated with IVF program among infertile couples in developing countries is the high cost of the IVF services.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"12 1","pages":"147 - 154"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43699593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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