Yasmin Sabina Sa’diah, Agung Dewanto, L. A. Chandra
{"title":"Efficacy of intraovarian autologous platelet-rich plasma administration in women with low ovarian reserve: A systematic review and meta-analysis","authors":"Yasmin Sabina Sa’diah, Agung Dewanto, L. A. Chandra","doi":"10.4103/apjr.apjr_141_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_141_23","url":null,"abstract":"\u0000 \u0000 To conduct a systematic examination and meta-analysis of the most reliable data from experimental studies evaluating the efficacy of autologous platelet-rich plasma (PRP) on low ovarian reserve.\u0000 \u0000 \u0000 \u0000 A comprehensive search was performed utilizing pertinent search terms across electronic databases, including PubMed, Cochrane, and Google Scholar. We included studies that assigned infertile women with low ovarian reserve in experimental studies. Ovarian reserve parameters were measured before and after PRP injection into ovaries. The data of each study was retrieved and subsequently compiled.\u0000 \u0000 \u0000 \u0000 Of 301 articles collected and reviewed, six studies were finally included in the meta-analysis. Following PRP injection, infertile women showed a non-significant increase in anti-Mullerian hormone (AMH) level (MD=0.10; 95% CI -0.04, 0.23), a significant increase in antral follicular count (AFC) (MD=1.88; 95% CI 0.47, 3.29), and a non-significant reduction in follicle-stimulating hormone (FSH) level (MD=-0.22; 95% CI -8.32, 7.87).\u0000 \u0000 \u0000 \u0000 Autologous PRP may increase AFC, but not AMH. Although it is found beneficial in enhancing ovarian reserve (AFC), further research with strong evidence is still required.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140402409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Semen features for conventional IVF: Need for a simple universal index","authors":"M. Işıkoğlu","doi":"10.4103/apjr.apjr_152_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_152_23","url":null,"abstract":"","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140406944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Adiponectin orchestrates testosterone suppression in biological pathways","authors":"Ekhoye Ehitare Ikekhuamen, Imonna Kingsle","doi":"10.4103/apjr.apjr_162_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_162_23","url":null,"abstract":"This current review highlights adiponectin engagement with AdipoRl and AdipoR2 which subsequently triggers pathways such as AMPK, PPARα, and MAPK, thereby modulating testicular steroidogenesis. Adiponectin's actions on Leydig and adrenal cells inhibit androgen secretion by suppressing the steroidogenic acute regulatory protein (StAR). Given that StAR facilitates cholesterol to testosterone conversion, AMPK inhibits this process by modulating cholesterol transport and suppressing StAR expression through multiple avenues. Furthermore, adiponectin-induced PPARα activation impedes mitochondrial cholesterol influx, further modulating androgen biosynthesis. The suppressive influence of PPARα on steroidogenic genes, notably StAR, is evident. Collectively, adiponectin signalling predominantly attenuates androgen production, ensuring metabolic and reproductive equilibrium. Imbalances, as seen in conditions like hypogonadism and obesity-related infertility, highlight their crucial roles and potential clinical interventions for reproductive disorders.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140407810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Perumal, J. Sunder, A. De, A. Nahak, D. Chaurasia, T. C. Balamurugan, D. Bhattacharya
{"title":"Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem","authors":"P. Perumal, J. Sunder, A. De, A. Nahak, D. Chaurasia, T. C. Balamurugan, D. Bhattacharya","doi":"10.4103/apjr.apjr_159_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_159_23","url":null,"abstract":"\u0000 \u0000 To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.\u0000 \u0000 \u0000 \u0000 A total of 25 semen samples from six bucks were selected for the study. Spermatozoa of 150× 106 were incubated in 0, 300, 500 and 700 μg of leaf extract as group I, II, III and IV, respectively. Liquid stored semen samples were analysed for motility, viability, total sperm abnormalities, plasma membrane, acrosomal and nuclear integrities, seminal plasma intracellular enzymes [aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH)] and total antioxidant capacity (TAC) and spermatozoa malondialdehyde (MDA) and cholesterol efflux in comparison with the control group (group I) for up to 72 h.\u0000 \u0000 \u0000 \u0000 \u0000 Moringa oleifera leaf extract (500 μg/150× 106 spermatozoa)-treated semen had significantly higher motility, viability, plasma membrane, acrosomal and nuclear integrities, and TAC (P<0.05), and had significantly lower total sperm abnormalities, AST, ALT, LDH, MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated (300 or 700 μg/150×106 spermatozoa) and control groups at different hours of liquid storage (P<0.05). Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities, MDA production, leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group I to group III and then an opposite trend from group II to group IV at different hours of liquid storage.\u0000 \u0000 \u0000 \u0000 \u0000 Moringa oleifera leaf extract 500 μg/150×106 spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140407291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Covid-19 vaccination in pregnancy and placental pathology","authors":"Leo Simanjuntak","doi":"10.4103/apjr.apjr_94_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_94_23","url":null,"abstract":"\u0000 \u0000 To describe and determine the association between Covid-19 vaccination in pregnancy and placental pathology.\u0000 \u0000 \u0000 \u0000 Conducted in a tertiary hospital in Medan, Indonesia, from April 30th 2022 to June 30th 2022, this single-center cross-sectional study involved Covid-19 vaccinated and unvaccinated women with singleton full-term pregnancies delivering live fetuses via cesarean section. Maternal characteristics, placental pathologies, and the placental index were documented at enrollment. The association between Covid-19 vaccination status and placental pathology was assessed.\u0000 \u0000 \u0000 \u0000 The study enrolled 200 pregnant women, including 110 vaccinated women and 90 unvaccinated women. No significant differences were observed in birthweight (P=0.48), placental index (P=0.48), and placental pathology findings [intervillous bleeding (P=0.20), increased syncytial knots (P=0.83), chorangiosis (P=0.13), villous stromal edema (P=0.13), vascular dilation and congestion (P=0.13), and vascular wall thrombus (P=0.71)] between the vaccinated and unvaccinated groups.\u0000 \u0000 \u0000 \u0000 This study revealed no statistically significant association between Covid-19 vaccination and placental pathology. The findings support the safety of Covid-19 vaccination during pregnancy, in regards to changes of the placental pathology.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140399024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rohollah Nazari, Somayeh Akbari, Maryam Naseh, Shayan Yousufzai, S. F. Hosseini, Fatemeh Karimi
{"title":"Protective effect of co-enzyme Q10 on testicular tissue and sperm parameters in adult male rats treated with Sunset Yellow FCF","authors":"Rohollah Nazari, Somayeh Akbari, Maryam Naseh, Shayan Yousufzai, S. F. Hosseini, Fatemeh Karimi","doi":"10.4103/apjr.apjr_55_23","DOIUrl":"https://doi.org/10.4103/apjr.apjr_55_23","url":null,"abstract":"\u0000 \u0000 To determine the protective effect of co-enzyme Q10 (CoQ10) on testicular tissue and sperm parameters in male rats treated with Sunset Yellow FCF.\u0000 \u0000 \u0000 \u0000 Sixty male Sprague-Dawley rats were randomly divided into 6 groups of the control, CoQ10 (10 mg/kg/day), low dose of Sunset Yellow (2.5 mg/kg), high dose of Sunset Yellow (70 mg/kg), low dose of Sunset Yellow (2.5 mg/kg) plus CoQ10, and high dose of Sunset Yellow (70 mg/kg) plus CoQ10. The drugs were administered via daily oral gavages for 6 weeks. At the end of the experiment, sperm analysis, stereological and histological assessments of the testis were carried out.\u0000 \u0000 \u0000 \u0000 The normal morphology (by 41.1%) and progressive spermatozoa (by 74.8%), testicle volume (by 33.4%), lumen volume (by 38.3%), interstitial tissue volume (by 44.7%), seminiferous tubule volume (by 40.7%), and number of spermatogonia (by 53.9%) and Leydig cells (by 70.7%) reduced in the rats that received high doses of Sunset Yellow in comparison to the control group. Nonetheless, all these alterations were recovered by CoQ10 treatment in the CoQ10 plus high dose of Sunset Yellow group. Furthermore, low doses of Sunset Yellow did not affect different parameters of the testis and sperm.\u0000 \u0000 \u0000 \u0000 CoQ10 could, to some extent, prevent structural changes of the testis induced by the high dose of Sunset Yellow.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140400059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial","authors":"N.S.V. Le, Minh Tam Le, T. N. Cao","doi":"10.4103/2305-0500.390300","DOIUrl":"https://doi.org/10.4103/2305-0500.390300","url":null,"abstract":"\u0000 \u0000 To compare the effectiveness of inositol and metformin on the clinical characteristics, and endocrine and metabolic profiles of infertile polycystic ovarian syndrome (PCOS) women from Vietnam.\u0000 \u0000 \u0000 \u0000 From June 2018 to August 2022, a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome. The clinical, endocrine, and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day. Natural pregnancy rates, adverse effects, and tolerance of inositol were recorded.\u0000 \u0000 \u0000 \u0000 The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment, of whom 132 women participated in data analysis after 3 months. After metformin treatment, 42.1% of women with oligomenorrhea experienced regular menstruation. Metformin significantly lowered body mass index (BMI), waist circumference and testosterone levels, but had no effect on other clinical characteristics, endocrine profiles, or metabolic profiles. 29.2% Of women reported experiencing side effects. 21% Of them attained pregnancy, which resulted in 17.1% of live births. In the inositol group, the rate of regular cycle increased by 18.2% and the total testosterone concentration significantly decreased. In overweight/obese women with PCOS, inositol significantly decreased weight, BMI, waist and hip circumferences (P<0.05). 100% Of women tolerated inositol and continued treatment. 18.9% Of them became pregnant, leading to 17% of live births.\u0000 \u0000 \u0000 \u0000 Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS. Metformin is associated with a higher rate of regular menstruation, whereas inositol is associated with a lower rate of adverse effects. The spontaneous conception, clinical pregnancy, and live birth rates between two groups are comparable.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138993025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh
{"title":"Embryonic, genetic and clinical outcomes of fresh versus vitrified oocyte: A retrospective cohort study","authors":"Phuong Thi Dao, Son Truong Dang, Thuan Duc Nguyen, Anh Van Pham, Anh Tuan Do, Nguyen Van Hanh","doi":"10.4103/2305-0500.390301","DOIUrl":"https://doi.org/10.4103/2305-0500.390301","url":null,"abstract":"\u0000 \u0000 To compare embryonic development, ploidy status and clinical outcomes between fresh and frozen-thawed oocytes.\u0000 \u0000 \u0000 \u0000 This retrospective cohort study evaluated 83 fertilization cycles including both fresh and frozen oocytes from 79 patients at the HP Fertility Center of Hai Phong International Hospital of Obstetrics and Pediatrics in Vietnam. The patient underwent several ovarian stimulation cycles to accumulate a certain number of oocytes that would be vitrified. In the last oocyte retrieval, all patient's oocytes including both frozen and fresh would be fertilized. The outcomes included the rates of oocyte survival, cleavage embryo, blastocyst, ploidy status, pregnancy, biochemical pregnancy and clinical pregnancy.\u0000 \u0000 \u0000 \u0000 The oocyte survival rate after thawing was 96.5%. No statistically significant difference was found when comparing fresh and frozen oocytes regarding fertilization rate (78.1% vs. 75.5%, P=0.461), usable cleavage embryo rate (86.9% vs. 87.2%, P=0.916) but usable blastocyst rate was found higher statistically in the frozen oocyte group (44.4% vs. 54.0%, P=0.049). The percentages of euploid, aneuploid and mosaic embryos between the fresh group and the vitrified group had no significant differences (33.8% vs. 31.6%, P=0.682; 51.0% vs. 54.2%, P=0.569; 15.2% vs. 12.4%, P=0.787; respectively). The rates of pregnancy, biochemical pregnancy and clinical pregnancy had no statistical difference (68.8% vs. 64.8%, P=0.764; 12.5% vs. 3.6%, P=0.258; 37.5% vs. 46.4%, P=0.565). 17 Mature oocytes are the minimum to have at least one euploid embryo.\u0000 \u0000 \u0000 \u0000 Oocyte vitrification does not affect embryonic, genetic and clinical results. The number of mature oocytes should be considered for fertilization in some cases.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra
{"title":"Cryopreserved ovine spermatogonial stem cells maintain stemness and colony forming ability in vitro","authors":"R. K. Pramod, Deepthi Varughese, A. J. Jameel, Bhisma Narayan Panda, Soma Goswami, Abhijit Mitra","doi":"10.4103/2305-0500.390302","DOIUrl":"https://doi.org/10.4103/2305-0500.390302","url":null,"abstract":"\u0000 \u0000 To assess the effect of cryopreservation on stemness and proliferation potential of sheep spermatogonial stem cells (SSCs) in vitro.\u0000 \u0000 \u0000 \u0000 Sheep testicular cells were isolated and putative SSCs were enriched by the laminin-based differential plating method. Putative SSCs were co-cultured with the Sertoli cell feeder prepared by the Datura Stramonium Agglutinin (DSA-lectin)-based method. The cultured putative SSCs were cryopreserved in Dulbecco's Modified Eagle Medium-10% fetal bovine serum mixture (DMEM-10% FBS) media containing 10% dimethyl sulfoxide (DMSO) alone or 10% DMSO plus 200 mM trehalose. Cryopreserved putative SSCs were evaluated for their proliferation potential using in vitro culture and stemness by immunocytochemistry. Finally, the transfection ability of cryopreserved putative SSCs was analyzed.\u0000 \u0000 \u0000 \u0000 We isolated 91% viable testicular cells from sheep testes. The majority of the laminin enriched cells expressed the SSC related marker, ITGA6. Co-culture of sheep putative SSCs with Sertoli cell feeder resulted in the generation of stable colonies, and the expression of SSC marker was maintained after several passages. A significantly higher number of viable putative SSCs was recovered from SSCs cryopreserved in media containing 10% DMSO and 200 mM trehalose compared to 10% DMSO alone (P<0.01). Cryopreserved putative SSCs formed colonies and showed SSC marker expression similar to the non-cryopreserved putative SSCs. The appearance of green fluorescent colonies over the Sertoli cell feeder indicated that cryopreserved sheep SSCs were successfully transfected.\u0000 \u0000 \u0000 \u0000 Cryopreserved putative SSCs can retain their stemness, colony forming ability, and transfection efficiency in vitro. Our research may help in the effective preservation of germplasm and the generation of transgenic ovine species.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar
{"title":"Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands","authors":"P. Perumal, J. Sunder, A. De, D. Bhattacharya, A. Nahak, R. Vikram, E. B. Chakurkar","doi":"10.4103/2305-0500.390304","DOIUrl":"https://doi.org/10.4103/2305-0500.390304","url":null,"abstract":"\u0000 \u0000 To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons.\u0000 \u0000 \u0000 \u0000 Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05).\u0000 \u0000 \u0000 \u0000 Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.\u0000","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}