Asian Pacific Journal of Reproduction最新文献

{"title":"Etiopathogenesis of reproductive tract infections and the emerging role of bitter taste receptors: A scoping review","authors":"M. Welcome, Abraham Jeremiah, D. Allagoa, S. Dane, Vladimir A. Pereverzev","doi":"10.4103/2305-0500.321122","DOIUrl":"https://doi.org/10.4103/2305-0500.321122","url":null,"abstract":"Reproductive tract infections pose an immense public health concern worldwide as over 600 million new cases are recorded annually along with several complications, including infertility, ectopic pregnancy, preterm delivery, and neonatal death. Despite improved understanding of the mechanisms of pathogenic invasion, the etiopathogenesis of reproductive tract infections is yet to be completely understood. Recent data has suggested a critical role of bitter taste receptors of the reproductive tract in etiopathogenesis of reproductive tract infections. Here, we review the literature on current etiopathogenesis of reproductive tract infections and the role of bitter taste receptors of the reproductive tract in etiopathogenesis of reproductive tract infections. Emerging evidence suggests a critical role of microbiota disorder of the reproductive tract in reproductive tract infections. Several bacterial, protozoan parasitic, helminthic parasitic and viral pathogens have been identified as causative agents of reproductive tract infections. These pathogens subvert host defenses and activate specific architectural units of the uroepithelium such as Toll-like receptors, which recognize conserved motifs on the pathogens. The activated Toll-like receptors mediate downstream signaling, stimulating nuclear factor-κB, which in turn activates the production of proinflammatory cytokines. This pathway is also associated with recruitment of immunocytes to the site of aggression and release of proteinases, which drive tissue damage in the reproductive tract. Defects in detection of pathogenic components by the bitter taste receptors of the reproductive tract may play a critical role in the etiopathogenesis of reproductive tract infections. This review provides important information for identification of novel frontiers for the treatment of reproductive tract infections.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46377423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks","authors":"K. Lukusa, J. Kabuba","doi":"10.4103/2305-0500.321126","DOIUrl":"https://doi.org/10.4103/2305-0500.321126","url":null,"abstract":"Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41563857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Descriptive histomorphological evaluation of the testis and caudal epididymis following treatment with rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) and sutherlandia (Lessertia frutescens) in healthy and streptozotocin-induced diabetic rats","authors":"Temidayo S. Omolaoye, S. D. du Plessis","doi":"10.4103/2305-0500.321125","DOIUrl":"https://doi.org/10.4103/2305-0500.321125","url":null,"abstract":"Objective: To evaluate the testis and the cauda epididymis after treating both healthy and diabetic rats with rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia), and sutherlandia (Lessertia frutescens), respectively. Methods: Ninety male Wistar rats were randomly divided into nine groups (n=10 per group). Fifty rats were randomized to receive tap water, 0.1 M citrate buffer, 2.0% fermented rooibos, 4.0% fermented honeybush, or 0.2% unfermented sutherlandia. Diabetes was induced via a single intraperitoneal injection of streptozotocin (STZ) at 45 mg/kg in 40 rats. The diabetic rats were randomized to receive tap water, 2.0% fermented rooibos, 4.0% fermented honeybush, or 0.2% unfermented sutherlandia. Rats were sacrificed after seven weeks of diabetic induction. Blood was collected for hormonal assay, while the testes and caudal epididymis were retrieved for histomorphological evaluations. Results: Diabetic rats treated with 2.0% fermented rooibos exhibited a significant increase in fasting plasma glucose (FPG) versus the vehicle group (P=0.002), and a 3.6% decrease compared to the diabetic control group (P>0.05). Diabetic rats treated with honeybush or sutherlandia showed 23.6% and 15.4% decrease in FPG when compared to the diabetic control group, respectively (both P>0.05). The diabetic control rats had a significant decrease in the percentage of histologically normal seminiferous tubules compared to the control group (P=0.049). There was 21.0%, 14.0%, and 5.4% rise in the percentage of normal seminiferous tubules in diabetic rats receiving rooibos, honeybush or sutherlandia, respectively, compared to the diabetic control group. The infusion control groups (rooibos, honeybush and sutherlandia) showed normal seminiferous tubules, presence of spermatozoa in the epididymal lumen, and had normal overall architecture. Both testicular and epididymal morphology were altered in the diabetic control group, but these disruptions were mildly ameliorated by rooibos, honeybush, and sutherlandia. Conclusions: The detrimental effects of diabetes on the histomorphological architecture of the testis and epididymis are mildly ameliorated by the infusions (2.0% fermented rooibos, 4.0% fermented honeybush, and 0.2% unfermented sutherlandia).","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47223525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposed age-stratified reference intervals of FSH derived from normozoospermic men","authors":"E. Waller, J. Conceicao, P. Matson, J. Yovich","doi":"10.4103/2305-0500.321188","DOIUrl":"https://doi.org/10.4103/2305-0500.321188","url":null,"abstract":"Objective: To demonstrate that serum follicle-stimulating hormone (FSH) in men rises with age, and to explore FSH reference intervals of age-related partitioning. Methods: Men aged 20-50 years (n=1 190) underwent semen analysis according to World Health Organization (2010) methods. Serum was frozen prior to measurement of FSH by using the Siemens ADVIA Centaur® XP immunoassay system. FSH central 95% intervals after logarithmic transformation based on age were derived from 1 037 normozoospermic men. These were then applied to oligozoospermic and azoospermic men. Men producing azoospermic semen samples were further classified as having non-obstructive azoospermia by clinical diagnostic criteria, including genetic analysis and surgical exploration. Results: Serum FSH in normozoospermic men increased with age (P<0.05), and reference intervals were determined with 10-year brackets: 21-30 years [(1.0-8.2) IU/L], 31-40 years [(1.4-9.5) IU/L], 41-50 years [(1.9-12.0) IU/L]. The proportion of oligozoospermic men with normal FSH concentrations was less than the normozoospermic men, which in turn was lower among azoospermic men (both P<0.01). The azoospermic men were further broken down according to the nature of the azoospermia as either obstructive or non-obstructive azoospermia, and 86.4% (38/44) men with non-obstructive azoospermia had elevated serum FSH concentrations whereas only 6.7% (1/15) men with obstructive azoospermia had high FSH levels, and this was significantly different (P<0.01). Conclusions: FSH concentrations increase in men between 20-50 years, and clinical interpretation of serum FSH results in men must be made by using age-based reference intervals.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42860398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxifolin attenuates ischemia-reperfusion induced oxidative ovarian damage in rats","authors":"S. Kiremitli, T. Kiremitli, U. Nayki, N. Yilmaz, C. Turkler, K. Dinç, R. Mammadov, G. Yazici, M. Gulaboglu, N. Cetin","doi":"10.4103/2305-0500.321124","DOIUrl":"https://doi.org/10.4103/2305-0500.321124","url":null,"abstract":"Objective: To investigate preventive effects of taxifolin on ischemia-reperfusion induced oxidative ovarian damage in rats. Methods: A total of 18 female Wistar albino rats were randomly and equally divided into three groups: the sham group, the ovarian ischemia reperfusion group, and the 50 mg/kg taxifolin+ovarian ischemia reperfusion group. The ovarian ischemia reperfusion and taxifolin+ovarian ischemia reperfusion groups were exposed to ischemia for 2 h and then followed by two-hour reperfusion protocol. Biochemical and histopathologic examinations were performed on the extracted ovaries. Results: Levels of malondialdehyde and cyclooxygenase-2 were increased, while reduced-glutathione and cyclooxygenase-1 were decreased in the ovarian ischemia reperfusion group. However, these values were reversed in the taxifolin+ovarian ischemia reperfusion group. Similarly, the number of primordial and developing follicules decreased in the ovarian ischemia reperfusion group, while they were within normal range in the taxifolin+ovarian ischemia reperfusion group. Conclusions: Ischemia followed by reperfusion leads to oxidative stress-related ovarian injury, and taxifolin may be useful for protecting ovarian tissue from such injury.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47821550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic association of rs7754840 and rs7756992 polymorphisms in the CDKAL1 gene and gestational diabetes mellitus in selected Filipino pregnant women","authors":"M. Pineda-Cortel, Karlo Baybayan, Peter Bello, Latiffa Camenforte, S. Ching, Kathleen Conti, Jeremiah Ignacio, J. Diaz, Adrian Villavieja, J. Pandac, E. Alejandro","doi":"10.4103/2305-0500.321123","DOIUrl":"https://doi.org/10.4103/2305-0500.321123","url":null,"abstract":"Objective: To investigate the possible association between rs7754840 and rs7756992 polymorphisms of CDKAL1 gene and susceptibility to gestational diabetes mellitus (GDM) in a Filipino pregnant population. Methods: A total of 101 patients with GDM and 99 women without GDM were included. Two CDKAL1 gene single nucleotide polymorphisms (SNPs), namely rs7754840 and rs7756992, were genotyped by using TaqMan allelic discrimination assays. Mann-Whitney U test, median and interquartile range were used to describe physical and biochemical characteristics. The differences in the genotype and allele distribution of the target genetic variants among the two groups of participants were assessed by using Chi-square test. Conformity to Hardy-Weinberg equilibrium was tested prior to conducting further analysis. Multiple logistic regression model was used to investigate the effects of the genotype models on GDM development. Results: There was no observed correlation between the genotypes of the rs7754840 SNP and oral glucose tolerance test parameters. Consequently, there was no significant association between genetic models of the rs7754840 SNP and GDM risk (additive OR 1.43, 95% CI 0.82-2.50, P=0.21; dominant OR 1.21, 95% CI 0.57-2.59, P=0.62; recessive OR 1.63, 95% CI 0.86-3.09, P=0.13). Conclusions: The results of this study suggest no association between CDKAL1 gene variant rs7754840 and GDM development in Filipino pregnant women. Further studies with a larger population should be performed to validate our findings.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49085914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coronaviruses in pregnant women in Saudi Arabia: A systematic comparative review of MERS-CoV and SARS-CoV-2","authors":"M. Barry, Magdoleen Barry","doi":"10.4103/2305-0500.316621","DOIUrl":"https://doi.org/10.4103/2305-0500.316621","url":null,"abstract":"Objective: To compare the effects of coronaviruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV) or coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), on pregnant women in Saudi Arabia, and to understand the disease dynamics of these coronaviruses so as to konw how to provide care and management of infected pregnant women and infants. Methods: We conducted a systematic review of all published papers on MERS-CoV and SARS-CoV-2 infections in pregnant women in Saudi Arabia to identify knowledge gaps. Inclusion and exclusion criteria were based on the PRISMA system. The search included all papers which were published between September 1, 2012 and November 29, 2020 on pregnant women with MERS-CoV and/ or COVID-19 in Saudi Arabia. Of 26 papers screened, five were included in the analysis. Results: A total of 11 pregnant women with MERS-CoV and four with COVID-19 were reported in Saudi Arabia in the medical literature during the review period. The mean ages of the women were 33 and 31 years old for MERS-CoV and COVID-19, respectively. Maternal and fetal mortality in cases of MERS-CoV were 35% and 30%, respectively, while no maternal or fetal mortalities were reported in COVID-19 cases. Conclusions: Very limited data has emerged from Saudi Arabia on pregnant women MERS-CoV and COVID-19. With such high mortality observed with MERS-CoV compared to COVID-19, there is a need for greater reporting of cases to truly grasp the extent of these infections in pregnant women in a country where both coronaviruses are circulating.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44376813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of flaxseed supplementation on metabolic state, endocrine profiles, body composition and reproductive performance of sows","authors":"Sukhjinder Kaur, A. Singh, M. Honparkhe, Ajeet Kumar, Prahlad Singh, U. Singh","doi":"10.4103/2305-0500.316625","DOIUrl":"https://doi.org/10.4103/2305-0500.316625","url":null,"abstract":"Objective: To assess the effect of flaxseed supplementation on metabolic profile, endocrine concentrations, non-esterified fatty acids (NEFA), body composition variables, and reproductive performance of sows. Methods: All the 21 crossbred Large White Yorkshire sows were considered in the study period starting at day 1 of current farrowing when the feeding of specific supplemental ration was started until the day of subsequent farrowing (days 150-155) and were equally allocated into three groups. Group 1 served as the control group and followed their normal feeding schedule. Group 2 and group 3, in addition to their normal feeding schedule, were supplemented with flaxseed at a rate of 0.5% and 1.0% of the dry matter, respectively. Blood samples were collected 15 days prior to farrowing, on the day of farrowing (day 0), at weekly intervals until day 28 of lactation and at monthly intervals during gestation to harvest the plasma. Plasma was used to assess the metabolic and endocrine status of sows. Body weight of each sow and individual birth weight of all piglets born were measured. Results: Flaxseed supplementation led to decrease in plasma cholesterol and triglyceride levels in the supplemented groups than in the control group (P<0.05). Plasma estradiol-17β level was higher in group 2 than that in group 1 and 3 on day 90 of the gestation period (P<0.05). The mean plasma level of insulin-like growth factor 1 was higher in group 3 than that in group 1 and 2 both in late lactation (day 28) as well as in early gestation (day 30) (P<0.05). Plasma NEFA and weight gain were greater in sows of group 2 and 3 compared to those fed with the normal control diets (P<0.05). The proportion of pregnant sows relative to sows bred was 100.0% in group 2 and 3 and 85.7% in the control group. Piglet mortality was lower in group 2 and 3 compared to group 1 (P>0.05). Conclusions: Flaxseed improves endocrine profiles, NEFA concentrations and body weight, resulting in better pregnancy rate and litter size.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46193143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats","authors":"Joseph Dare, A. Ojewale, O. Olaniyan, John Adole, G. Okotie, C. Akintayo, A. Dare","doi":"10.4103/2305-0500.316623","DOIUrl":"https://doi.org/10.4103/2305-0500.316623","url":null,"abstract":"Objective: To evaluate the effects of aqueous leaf extract of Adansonia (A.) digitata L on dexamethasone-induced testicular damage in male Wistar rats. Methods: Twenty adult male Wistar rats weighing 170-190 g were divided into four groups. Group I received 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group; group II received 10 mg/kg of dexamethasone (a synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days, group III received 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A. digitata extract orally for 21 days; group IV received 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days. Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days. The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested. The testis was homogenized in 0.25 M sucrose at 4 °C for biochemical and histological analyses. Results: Administration of dexamethasone significantly decreased body weight, glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P<0.05), and significantly increased malondialdehyde (MDA) activities (P<0.05). The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone. However, administration of A. digitata significantly increased SOD, GPx, G6PDH, and LDH levels, significantly decreased MDA activities and improved the histoarchitecture of the testis (P<0.05). Conclusions: A. digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45828956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of royal jelly on in vitro fertilization and early embryo development following nicotine treatment in adult female rats","authors":"Z. Khodabandeh, V. Nejati, A. Shalizar-Jalali, G. Najafi, F. Rahmani","doi":"10.4103/2305-0500.316624","DOIUrl":"https://doi.org/10.4103/2305-0500.316624","url":null,"abstract":"Objective: To scrutinize the protective role of royal jelly as an antioxidant on nicotine-induced changes in malondialdehyde (MDA) level, p53 expression, in vitro fertilization (IVF) rate, and early embryo development in adult female rats. Methods: A total of 56 adult female Wistar rats were divided into 8 groups (n=7 in each group). Group 1 served as an untreated control group, group 2, 3 and 4 received nicotine at a dose of 0.50, 1.00 and 2.00 mg/kg respectively, group 5 received royal jelly at a dose of 100.00 mg/kg, and group 6, 7 and 8 received 0.50, 1.00 and 2.00 mg/kg nicotine, respectively, with 100.00 mg/kg body weight royal jelly. Nicotine and royal jelly were administered daily for 49 days in the experimental groups intra-peritoneally and orally, respectively. At the end of the experimental period, p53 expression, IVF rate and early embryo development as well as MDA concentration were measured. Results: The IVF rate, number of cumulus oocytes, two-cell embryos and blastocysts decreased in the nicotine-treated groups in a dose-dependent manner. In addition, p53 mRNA expression and MDA levels increased in the nicotine-treated groups. Royal jelly co-administration led to partial improvement in the aforementioned parameters. Conclusions: Royal jelly may have a repro-protective effect in nicotine-administered female rats in terms of its anti-oxidant and anti-apoptotic properties.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44902110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}