洋地黄水叶提取物对地塞米松致雄性Wistar大鼠睾丸损伤的改善作用

IF 0.5 Q4 REPRODUCTIVE BIOLOGY
Joseph Dare, A. Ojewale, O. Olaniyan, John Adole, G. Okotie, C. Akintayo, A. Dare
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引用次数: 0

摘要

目的:探讨花楸叶水提物对地塞米松致雄性Wistar大鼠睾丸损伤的影响。方法:体重170 ~ 190 g的成年雄性Wistar大鼠20只,随机分为4组。ⅰ组患者口服磷酸盐缓冲液0.5 mL,连续28 d,作为正常对照组;II组患者腹腔注射地塞米松(一种合成糖皮质激素)10 mg/kg,连续7天,口服磷酸盐缓冲液0.5 mL,连续21天;III组患者口服地塞米松10 mg/kg,连续7天,口服地塞米松提取物800 mg/kg,连续21天;IV组给予地塞米松10 mg/kg口服7 d,维生素e 300 mg/kg口服21 d。地塞米松腹腔给药7 d,全部给药28 d。用乙醚麻醉处死大鼠,取各组睾丸。睾丸用0.25 M蔗糖在4°C下匀浆,进行生化和组织学分析。结果:地塞米松显著降低体重、葡萄糖-6-磷酸脱氢酶(G6PDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性(P<0.05),显著提高丙二醛(MDA)活性(P<0.05)。地塞米松给药后大鼠精原细胞群变性、空泡形成、间质异常增宽。而给药后睾丸组织SOD、GPx、G6PDH、LDH水平显著升高(P<0.05), MDA活性显著降低(P<0.05),组织结构明显改善(P<0.05)。结论:地塞米松对Wistar大鼠睾丸损伤的影响可能与地塞米松抗炎、抗氧化作用有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats
Objective: To evaluate the effects of aqueous leaf extract of Adansonia (A.) digitata L on dexamethasone-induced testicular damage in male Wistar rats. Methods: Twenty adult male Wistar rats weighing 170-190 g were divided into four groups. Group I received 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group; group II received 10 mg/kg of dexamethasone (a synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days, group III received 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A. digitata extract orally for 21 days; group IV received 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days. Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days. The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested. The testis was homogenized in 0.25 M sucrose at 4 °C for biochemical and histological analyses. Results: Administration of dexamethasone significantly decreased body weight, glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P<0.05), and significantly increased malondialdehyde (MDA) activities (P<0.05). The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone. However, administration of A. digitata significantly increased SOD, GPx, G6PDH, and LDH levels, significantly decreased MDA activities and improved the histoarchitecture of the testis (P<0.05). Conclusions: A. digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.
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来源期刊
Asian Pacific Journal of Reproduction
Asian Pacific Journal of Reproduction Veterinary-Veterinary (all)
CiteScore
1.70
自引率
0.00%
发文量
588
审稿时长
9 weeks
期刊介绍: The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.
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