卵巢卵泡发育过程中 VEGF165 和 VEGF165b 的表达

IF 0.5 Q4 REPRODUCTIVE BIOLOGY

Asian Pacific Journal of Reproduction Pub Date : 2023-12-01 DOI:10.4103/2305-0500.390303

Chinnarat Changsangfa, Bongkoch Turathum, Morakot Sroyraya, Khwanthana Grataitong

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引用次数: 0

摘要

研究血管内皮生长因子（VEGF）165a、VEGF165b 和血管内皮生长因子受体（VEGFR）在牛卵泡发育过程中的作用。我们用雌激素培养了从小号、中号和大号牛卵泡中收集的卵泡细胞，并通过 Western 印迹分析和免疫荧光法测定了 VEGF、VEGFR2 和 VEGF165b 的表达。在含有雌激素的培养基中培养后，所有大小的卵泡中 VEGF165 的表达量都有所增加，小卵泡和大卵泡中 VEGF165b 的表达量也有所增加。这些证据表明，在牛卵泡发育过程中，VEGF165 和 VEGFR 的表达与雌激素刺激有关，并且是以自分泌或旁分泌的方式表达的。这揭示了体外卵母细胞成熟过程中的优势。

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Expression of VEGF165 and VEGF165b during ovarian follicular development

To investigate the role of vascular endothelial growth factor (VEGF)165a, VEGF165b, and VEGF receptor (VEGFR) in the development of bovine follicles. We cultured follicular cells that were collected from small, medium, and large sized bovine follicles with estrogen and measured the expression of VEGF, VEGFR2 and VEGF165b by Western blot analysis and immunofluorescence. The expression of VEGF165 increased in all follicle sizes and the expression of VEGF165b was increased in the small and large follicles after culturing in an estrogen containing medium. The expression of VEGFR2 was increased in the medium and large follicles after culturing with estrogen for 96 h. VEGF165 was activated at 100 ng/mL estrogen in the large follicles for 96 h. In addition, VEGFR2 was upregulated in the medium and large follicles after treated with 100 ng/mL estrogen for 96 h. This evidence suggests that the expression of VEGF165 and VEGFR is associated with estrogen stimulation during the development of bovine follicles and in an autocrine or paracrine manner. This reveals an advantage during oocyte maturation in vitro.

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来源期刊

Asian Pacific Journal of Reproduction Veterinary-Veterinary (all)

CiteScore

1.70

自引率

0.00%

发文量

588

审稿时长

9 weeks

期刊介绍： The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.