{"title":"Soil persistence of 4-HPPD-inhibitors in different soil types.","authors":"L Maeghe, H Eelen, R Bulcke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In field experiments carried out during the 1997-2001 period on four different soil types (sand, sandy loam, heavy sandy loam and clay) in Flanders (Belgium), the persistence of the three 4-HPPD inhibiting maize herbicides mesotrione (100 and 150 g ha-1), sulcotrione (300 and 450 g ha-1) and isoxaflutole (75 and 125 g ha-1) was studied. Therefore, soil samples were taken at regular intervals from application in spring and frozen. When all samples had been taken, greenhouse bioassays were set up to detect herbicide residues in the different soil types. Therefore, two extremely sensitive test plants, sugarbeet (Beta vulgaris L. spp. altissima Doell. var. saccharifera Deck.-Dill) and red clover (Trifolium pratense L.) were sown in the soil samples. Test plants were harvested after 2 (sugarbeet) and 3 (red clover) weeks and foliage fresh weight per plant was determined. This parameter was expressed relatively to the average fresh weight per plant of the plants sown in the control soil samples taken at each sampling date. The bioassays revealed several factors that influence the persistence of the herbicide tested. First, there is a remarkable influence of the experimental year due to variation in weather conditions (especially rainfall and temperature during the first weeks after spraying). Secondly, a different soil texture results in a highly different persistence: the shortest biological persistence was noticed each year in clay, followed by heavy sandy loam; the longest persistence was recorded in sandy and sandy loam soil types. Thirdly, there are important differences between the three herbicides tested: isoxaflutole (a member of the isoxazole chemical family) was shown to be less persistent than sulcotrione and mesotrione (both members of the triketone family). Remarkably, this was not the case in clay, where a longer persistence could be seen for isoxaflutole compared to sulcotrione and mesotrione. This study also revealed that applying a low rate results in a shorter persistence period compared to the higher rate. All these factors work together in a complex way which determines the persistence of the three herbicides tested.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 3","pages":"383-91"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22341646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Data on the biology of the crucifer flea beetle, Phyllotreta cruciferae (Goeze, 1777) (Coleoptera, Chrysomelidae, Alticinae).","authors":"Károly Vig","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Biology of Phyllotreta cruciferae (Goeze, 1777) known as serious pest of cruciferous crops in Europe and North America was investigated both in the laboratory and under field conditions in Southwestern Hungary. Adults overwinter under plant debris and appear in large number by the end of April-beginning of May. Eggs are laid from May through spring until mid-July. Larvae can be found from June to mid-October. The pupation lasts until October. The young adults of the new generation appear in August and retire to the overwintering sites by the end of October. There was one generation observed under Hungarian conditions. Considering results annual cycle of Phyllotreta cruciferae (Goeze, 1777) is presented. The average number of eggs deposited by females in laboratory was 200.25 +/- 67.2. Investigations concerning the effect of the temperature on individual's developing time were accomplished under three controlled temperatures. Length of the development under different temperature conditions, the biological zero point and DD value of different developmental stages of Phyllotreta cruciferae (Goeze, 1777) are presented.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 3","pages":"537-46"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22342077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Individual number change of Empoasca spp. cicadas species in potato.","authors":"A Polgár, G Kuroli, A Orosz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We investigated the individual number change of Empoasca spp. cicadas species that are living in potatoes for 3 years. We applied the \"100 plants\" method for the determination for the individual number once per week. Based on the data determined that the settling of adult begins on the last days of May, and valid presence will be followed till the end of the breeding-season. The individual number was gradually increased during the settling, and reached the peak in 1990 on 23 July, in 2000 on 6 August and in 2001 on 22 July. We determined the individuals gathered during the collection. In the population of Empoasca spp. The E. solani Curtis and the E. decipiens Paoli played the dominant role. The E. vitis Göthe was also present with insignificant individual number. Beside the adults many larvae and nymphs were living on the potato which means that the Empoasca spp. Can reproduce also on the potato. At the time of peak individual number in 1999 1876 larvae, 344 nymphs and 400 adults were present on 100 plants, in 2000 (according to the previous order) 3340, 580, 1280, and in 2001 there were 954, 786 and 285. The rainfall in Hungary was disadvantageous in the investigated period, especially the year 2000. During the rain-free period the temperature was high and the relative humidity of the air was low. However, we could state that the increase of the cicada individual number was not restrained by the heat (30-35 degrees C) days. The decrease was in connection with the ageing of the plant stock.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 3","pages":"547-56"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22342078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eric Haubruge, Flavie Vanlerberghe-Masutti, Pierre Collignon, Frédéric Francis
{"title":"The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of Coccinella septempunctata in Belgium.","authors":"Eric Haubruge, Flavie Vanlerberghe-Masutti, Pierre Collignon, Frédéric Francis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The movement and dispersion of Coccinella septempunctata and its efficacy as aphid control agent over large areas is not really understood because of the difficulty in identifying the origins of predators. To quantify the genetic diversity within the species and monitor the spatial foraging, populations were sampled from Belgium and analysed for RAPD DNA variation. Twenty decamer primers generated more than hundred polymorphic RAPD bands and pairwise distances were calculated between populations according to Nei and Li, then used to construct a radial neighbour-joining dendrogram and examine intra- and inter-population variance coefficients, by analysis of molecular variation (AMOVA). This study shows that while a number of factors can complicate the use and interpretation of RAPD fragments as genetic markers, RAPD analysis can be a valuable technique for studies of intra-specific genetic variation in C. septempunctata.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 3","pages":"557-61"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22342079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fusarium nygamai.A causal agent of root rot of Vicia faba L. in the Sudan.","authors":"A M Kurmut, H I Nirenberg, H Bochow, C Büttner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Wilted and rotted plants of Vicia faba were received from different localities in the Sudan. Among several Fusarium spp., Fusarium nygamai was recovered. Conspicuous symptoms were among others black root rot, associated with rot and death of the lateral root system. Severely infected plants showed black neck canker at soil level. These symptoms were usually accompanied by loss of the leaves' turgor, these then turned brown and died. Death of intact leaves also occurred. Most of the strains proved to be pathogenic to Vicia faba. Disease intensity varied between 28-100%. This is the first report of Fusarium nygamai as a pathogen of Vicia faba.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"269-74"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22345656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of some weeds as hosts of Capsicum viruses in the rift valley parts of Ethiopia.","authors":"Tameru Alemu, J Hamacher, H W Dehne","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hot pepper (Capsicum annuum. L.) is a traditionally and economically important spice crop in Ethiopia. Chronic infection with viruses is reported as major constraint, that often force farmers to ban its production. However, epidemiological studies on these viruses are lacking in the country. Knowledge of the weed flora as virus reservoir is essential to reduce the spread of plant viruses. The potential role of some frequently and abundantly occurring weed species as host plants for Capsicum viruses was therefore investigated. Surveys were conducted during the 2000/2001 cropping- and off-season. Samples of various weed species revealing virus symptoms were collected from different districts. Collection was done from inside and at the border of pepper cropped and fallow fields. It was also performed along road sides and irrigation channels. The identity of the collected isolates was established based on serology, electron microscopy and host range studies. Moreover, seeds were collected from natural populations of the most prevalent weeds, namely Datura stramonium and Nicandra physalodes. They were grown under glass house conditions and tested for their ability to host viruses. To verify their role further, healthy seedlings from these weeds were artificially inoculated with all viruses isolated from weeds and Capsicum leaf samples. Natural infection of different Potyviruses with an average incidence of 33% in the weed samples were the most dominant. In addition, the occurrence of Cucumber mosaic virus, Tomato mosaic virus and Potato virus X in these weeds was demonstrated. However, none of the isolated viruses was found to be seed borne in the tested weed species and pepper crop (var.Mrkofanna) under glass house conditions. Recently, there was an explosion in population size of some solanaceous weeds, particularly Datura and Nicandra species in the area. The presence of infected weeds throughout the year means, that they are an important reservoir and source for secondary spread. It is suggested therefore, that a rigorous weed control is an important step to reduce the incidence of viruses infecting Capsicum in the area.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"283-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22345659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the IPM Wheat Model in the Rhineland 2001.","authors":"M Heger, E C Oerke, J A Verreet, H W Dehne","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of field trials at nine locations with two wheat cultivars in each case demonstrated the practicability of the action threshold-based fungicide strategy of the IPM Wheat Model under the intensive wheat production conditions in the Rhineland. Action thresholds used and dosage of fungicides applied are outlined. Despite using reduced rates of fungicides the epidemic development of relevant pathogens--mainly Septoria tritici and Puccinia recondita--was controlled in early stages of disease development. In plots treated according to the IPM Wheat Model disease incidence and disease severity were reduced effectively compared to untreated plots with low and high disease incidence, respectively. The effective control of pathogens using pathogen-specific action thresholds resulted in yield levels very similar to those of disease-free plots simultaneously minimising the total amount of fungicides applied. Disease control according to the IPM Wheat Model raised yields by up to 25% with monetary benefits averaging more than [symbol: see text] 100 ha-1 in 2001.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"189-95"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22345691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Verticillium wilt of cauliflower in Belgium.","authors":"J Debode, K Spiessens, L De Rooster, M Höfte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Since several years commercial cauliflower in Belgium is severely affected by a vascular wilt disease. Plants wilt and a vascular discoloration of the cauliflower stems can be observed. The pathogen causes significant yield and quality losses. The first objective of this study was to isolate and characterise the causal agent of this disease. Verticillium species could be isolated out of the vascular tissue of symptomatic cauliflower plants. Morphological and physiological characteristics indicated that the isolates from cauliflower could be identified as the new and brassica-related species V. longisporum. Secondly, the susceptibility of several cauliflower cultivars to V. longisporum was evaluated by way of field experiments in naturally infested soil. At harvest, vascular discoloration was evaluated by a visual score. These experiments pointed to considerable differences between cultivars in their susceptibility to V. longisporum. The final goal of this study was to find a way to control the wilt disease on cauliflower. A field experiment showed that vascular discoloration of cauliflower was significantly reduced when soil was incorporated with broccoli residues, two Brassica green manures (B. juncea or B. nigra) or two commercial biological products based on Talaromyces flavus or Trichoderma sp..</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"241-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22345697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The SA-dependent defense pathway is active against different pathogens in tomato and tobacco.","authors":"A E Achuo, K Audenaert, H Meziane, M Höfte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The importance of salicylic acid (SA) in plant defense against pathogen attack has been elaborately documented. Benzothiadiazole (BTH, BION), a chemical analogue of SA, also induces resistance through the SA-dependent pathway. We investigated the role of SA in both basal defense and induced resistance of tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum) against Oidium neolycopersici (a biotrophic pathogen) and Botrytis cinerea (a necrotrophic pathogen). A comparison of NahG transgenic tomato and tobacco (unable to accumulate SA) to their respective wild types revealed that in both crops, SA was not involved in basal defense against O. neolycopersici. SA also played no role in the basal defense of tobacco against B. cinerea but NahG tomato plants were significantly more sensitive to B. cinerea than wild type plants. Activation of the SA-dependent defense pathway via BTH resulted in induced resistance against B. cinerea in tomato but not in tobacco. In contrast, BTH induced resistance against O. neolycopersci in tobacco but not in tomato. Microscopic analysis revealed that BTH treatment could prevent penetration of the odium germ tube through the epidermal cell wall of tobacco leaves whereas penetration was successful on tomato leaves, irrespective of BTH treatment. We conclude that even in two related plant species such as tomato and tobacco, the SA-dependent defense pathway does not trigger the same defense responses. It also means that the outcome of a BTH treatment cannot be predicted and has to be tested for each plant-pathogen combination.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"149-57"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22346435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of strobilurins on physiology and yield formation of wheat.","authors":"C Beck, E C Oerke, H W Dehne","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Strobilurin fungicides have a broad spectrum activity against all major foliar pathogens of wheat. In addition to this extraordinary fungicidal activity side-effects have been reported which result in higher yields of cereals, e.g. the reduction of respiration, delayed leaf senescence, activation of nitrogen metabolism as well as increased tolerance against abiotic stress factors. In the vegetation period 2000/2001 field trials were carried out at three sites in North Rhine-Westphalia to study the effects of three strobilurin fungicides on the yield formation of six winter wheat varieties. The strobilurins were applied two times as the commercial products Stratego (trifloxystrobin + propiconazole), Amistar/Pronto Plus (azoxystrobin/spiroxamine + tebuconazole) and Juwel Top (kresoxim-methyl + epoxiconazole + fenpropimorph. Fungicide-treated plants were kept disease-free by an initial azole-application in GS 31 in order to exclude disease effects on physiological parameters relevant to yield formation. Photosynthetic electron transport of strobilurin-treated wheat, was improved as early as at GS 65 compared to azole-treated plants. Differences often increased with growth stage and were closely related to a delay in leaf senescence. A higher photosynthetic activity of strobilurin-treated plants was confirmed by gas exchange and chlorophyll fluorescence measurements under field conditions. The yield benefit of wheat from strobilurin treatments varied from 2% to 9% depending on an improved photosynthetic capacity due to a higher and/or prolonged activity. Neither yield potential nor disease susceptibility of the cultivar had an effect on the height of the extra yield which, in contrast was modified by location and wheat genotype.</p>","PeriodicalId":85134,"journal":{"name":"Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)","volume":"67 2","pages":"181-7"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22346439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}