Asian Biomedicine最新文献

{"title":"Molecular genetic aberrations in the pathogenesis of multiple myeloma.","authors":"Ivyna Pau Ni Bong,&nbsp;Ezalia Esa","doi":"10.2478/abm-2023-0056","DOIUrl":"https://doi.org/10.2478/abm-2023-0056","url":null,"abstract":"<p><p>Multiple myeloma (MM) is the second most common form of blood cancer characterized by clonal expansion of malignant plasma cells within the bone marrow. MM is a complex, progressive, and highly heterogeneous malignancy, which occurs via a multistep transformation process involving primary and secondary oncogenic events. Recent advances in molecular techniques have further expanded our understanding of the mutational landscape, clonal composition, and dynamic evolution patterns of MM. The first part of this review describes the key oncogenic events involved in the initiation and progression of MM, together with their prognostic impact. The latter part highlights the most prominent findings concerning genomic aberrations promoted by gene expression profiling (GEP) and next-generation sequencing (NGS) in MM. This review provides a concise understanding of the molecular pathogenesis of the MM genome and the importance of adopting emerging molecular technology in future clinical management of MM.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"152-162"},"PeriodicalIF":0.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complementary medicine for fatigue: the importance of basic sciences and clinical studies.","authors":"","doi":"10.2478/abm-2023-0055","DOIUrl":"10.2478/abm-2023-0055","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"150-151"},"PeriodicalIF":0.4,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening anti-fatigue components of American ginseng saponin by analyzing spectrum-effect relationship coupled with UPLC-Q-TOF-MS.","authors":"Meiyu Lin,&nbsp;Shaiping Hu,&nbsp;Qi Zeng,&nbsp;Bixia Xiao,&nbsp;Yao He","doi":"10.2478/abm-2023-0057","DOIUrl":"https://doi.org/10.2478/abm-2023-0057","url":null,"abstract":"<p><strong>Background: </strong>American ginseng has an obvious anti-fatigue effect, but the effective material basis is still unclear. The spectrum-effect relationship is a scientific method that studies the correlations between chemical spectra and pharmacological effect.</p><p><strong>Objective: </strong>To reveal the real bioactive compounds in American ginseng saponin (AGS) based on a study of the underlying correlations between these compounds' occurrence in rat serum after their intake of AGS and the anti-fatigue effect of AGS.</p><p><strong>Methods: </strong>We utilized ultra-performance liquid chromatography (UPLC) with quadrupole and time-of-flight mass spectrometry (Q-TOF-MS) to analyze the extract of AGS and its constituents in serum after oral administration in rats. The anti-fatigue effect of AGS in rats was measured using the time weight-bearing swimming technique, the content of blood urea nitrogen, hepatic glycogen, and blood lactic acid. The relationship between the peak area values in fingerprints from rat serum and pharmacodynamic parameters of AGS was established using correlation analysis with partial least square regression (PLSR) method and gray correlation method.</p><p><strong>Results: </strong>We detected and identified 22 compounds from extract, and 8 prototype components from serum. Through PLSR and gray correlation method, it was found that the ginsenosides Re, Rb1, and Rb2 were significantly positively related to the pharmacodynamic data.</p><p><strong>Conclusions: </strong>Based on the spectrum-effect relationship, PLSR and gray correlation method can be used to screen for the anti-fatigue components available in AGS. Such an approach is of practical significance as it provides an effective means for exploring the material basis for the efficacy of American ginseng, particularly as an anti-fatigue agent.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"163-172"},"PeriodicalIF":0.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of coenzyme Q10 in a propofol infusion syndrome model of rabbits.","authors":"Banu Kilicaslan,&nbsp;Seda B Akinci,&nbsp;Fatma Saricaoglu,&nbsp;Savas O Yılbas,&nbsp;Burcu A Ozkaya","doi":"10.2478/abm-2023-0058","DOIUrl":"https://doi.org/10.2478/abm-2023-0058","url":null,"abstract":"<p><strong>Background: </strong>Coenzyme Q (CoQ) might be the main site of interaction with propofol on the mitochondrial respiratory chain in the propofol infusion syndrome (PRIS) because of the structural similarity between coenzyme Q10 (CoQ10) and propofol.</p><p><strong>Aim: </strong>To investigate the effects of CoQ10 on survival and organ injury in a PRIS model in rabbits.</p><p><strong>Methods: </strong>Sixteen male New Zealand white rabbits were divided into 4 groups: (1) propofol infusion group, (2) propofol infusion and CoQ10, 100 mg/kg was administered intravenously, (3) sevoflurane inhalation was administered, and (4) sevoflurane inhalation and CoQ10, 100 mg/kg intravenously, was administered. Arterial blood gas and biochemical analyses were repeated every 2 h and every 12 h, respectively. Animals that were alive on the 24th hour after anesthesia induction were euthanized. The organ damages were investigated under light and transmission electron microscopy (TEM).</p><p><strong>Results: </strong>The propofol infusion group had the highest troponin T levels when compared with the other three groups at the 12th hour. The propofol + CoQ10 group had lower troponin T levels when compared with the propofol and sevoflurane groups (<i>P</i> < 0.05). Administration of CoQ10 decreased total liver injury scores and total organ injury scores both in the propofol and sevoflurane groups. The propofol and sevoflurane organ toxicities were attenuated with CoQ10 in liver, gallbladder, urinary bladder, and spleen.</p><p><strong>Conclusion: </strong>The addition of CoQ10 to propofol and sevoflurane anesthesia prevented the propofol-associated increase in troponin T levels at the 12th hour of infusion and decreased anesthetic-induced total liver and organ injury scores.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"173-184"},"PeriodicalIF":0.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico prediction of deleterious non-synonymous SNPs in <i>STAT3</i>.","authors":"Athira Ajith,&nbsp;Usha Subbiah","doi":"10.2478/abm-2023-0059","DOIUrl":"https://doi.org/10.2478/abm-2023-0059","url":null,"abstract":"<p><strong>Background: </strong><i>STAT3</i>, a pleiotropic transcription factor, plays a critical role in the pathogenesis of autoimmunity, cancer, and many aspects of the immune system, as well as having a link with inflammatory bowel disease. Changes caused by non-synonymous single nucleotide polymorphisms (nsSNPs) have the potential to damage the protein's structure and function.</p><p><strong>Objective: </strong>We identified disease susceptible single nucleotide polymorphisms (SNPs) in <i>STAT3</i> and predicted structural changes associated with mutants that disrupt normal protein-protein interactions using different computational algorithms.</p><p><strong>Methods: </strong>Several <i>in silico</i> tools, such as SIFT, PolyPhen v2, PROVEAN, PhD-SNP, and SNPs&GO, were used to determine nsSNPs of the <i>STAT3</i>. Further, the potentially deleterious SNPs were evaluated using I-Mutant, ConSurf, and other computational tools like DynaMut for structural prediction.</p><p><strong>Result: </strong>417 nsSNPs of <i>STAT3</i> were identified, 6 of which are considered deleterious by <i>in silico</i> SNP prediction algorithms. Amino acid changes in V507F, R335W, E415K, K591M, F561Y, and Q32K were identified as the most deleterious nsSNPs based on the conservation profile, structural conformation, relative solvent accessibility, secondary structure prediction, and protein-protein interaction tools.</p><p><strong>Conclusion: </strong>The in silico prediction analysis could be beneficial as a diagnostic tool for both genetic counseling and mutation confirmation. The 6 deleterious nsSNPs of <i>STAT3</i> may serve as potential targets for different proteomic studies, large population-based studies, diagnoses, and therapeutic interventions.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"185-199"},"PeriodicalIF":0.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of transversus abdominis plane block application on postoperative analgesia quality and patient satisfaction after varicocele surgery: a randomized clinical trial.","authors":"Ömer Faruk Boran,&nbsp;Aykut Urfalioglu,&nbsp;Mahmut Arslan,&nbsp;Fatih Mehmet Yazar,&nbsp;Bora Bilal,&nbsp;Yavuz Orak,&nbsp;Bülent Katı,&nbsp;Ayşe Azak Bozan","doi":"10.2478/abm-2023-0053","DOIUrl":"https://doi.org/10.2478/abm-2023-0053","url":null,"abstract":"<p><strong>Background: </strong>Postoperative pain management is an important aspect of anesthesia care and multimodal analgesic techniques are generally recommended.</p><p><strong>Objective: </strong>To compare the effect of spinal anesthesia + transversus abdominis plane (TAP) block application on postoperative analgesia quality and patient satisfaction with spinal anesthesia + intrathecal morphine (ITM) application.</p><p><strong>Methods: </strong>A total of 70 patients were randomly separated into 2 groups as spinal anesthesia + TAP block (TAP block group, n = 34) and spinal anesthesia + ITM group (ITM group, n = 36). The groups were compared in respect of age, body mass index values, and visual analog scale (VAS) values at 0 h, 2 h, 6 h, 12 h, and 18 h, and patient satisfaction was scored by Quality Improvement in Postoperative Pain Management at 24 h.</p><p><strong>Results: </strong>The mean age of the patients was 32.52 ± 6.50 years in the TAP block group and 30.11 ± 5.62 years in the ITM group, with no statistically significant difference determined. There was no statistically significant difference in terms of VAS values at 0 h, 2 h, 6 h, 12 h, and 18 h. When the factors affecting postoperative patient satisfaction were evaluated, feeling fatigue after the surgery (r = -0.811, <i>P</i> = 0.001) and postoperative complications such as nausea, vomiting, and itching (r = -0.831, <i>P</i> = 0.001) were found to have a negative effect on patient satisfaction.</p><p><strong>Conclusion: </strong>Due to low complication rates, TAP block is an effective application for postoperative analgesia management in varicocele operations that increases patient satisfaction postoperatively.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"136-143"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-quality contact tracing, case investigation, and isolation of suspected and confirmed cases: a key strategy to control the spread of infection.","authors":"","doi":"10.2478/abm-2023-0049","DOIUrl":"10.2478/abm-2023-0049","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"93-94"},"PeriodicalIF":0.4,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A.","authors":"Xiaoyong Huang,&nbsp;Yuna Jia,&nbsp;Haiyan Shi,&nbsp;Haiyan Fan,&nbsp;Lingbo Sun,&nbsp;Huahua Zhang,&nbsp;Yanfeng Wang,&nbsp;Jie Chen,&nbsp;Jiaqi Han,&nbsp;Mingming Wang,&nbsp;Juan Du,&nbsp;Jing Zhang","doi":"10.2478/abm-2023-0052","DOIUrl":"https://doi.org/10.2478/abm-2023-0052","url":null,"abstract":"<p><strong>Background: </strong>The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the 3'-untranslated region (UTR) of the target gene, microRNAs (miRNAs) exert significant control over the expression of genes.</p><p><strong>Objectives: </strong>To investigate the influence of miR-30c-2-3p and DNA topoisomerase II alpha (TOP2A) on RCC growth and the mechanisms underlying the regulation of its expression.</p><p><strong>Methods: </strong>The expression of miRNA-30c-2-3p and <i>TOP2A</i> in RCC cells was examined using quantitative real-time polymerase chain reaction (qRT-PCR). MiR-30c-2-3p mimics, its inhibitors, and controls, as well as TOP2A short hairpin RNA (shRNA) and controls, were used to transfect the human RCC cell lines 786-O, Caki-1, and ACHN. Additionally, the roles of miRNA-30c-2-3p and TOP2A in the growth of RCC were evaluated using the cell counting kit (CCK)-8 test, colony formation assay, apoptosis analysis, and Western blotting. Meanwhile, binding of miRNA-30c-2-3p and TOP2A was verified using dual-luciferase reporter assays and Western blotting.</p><p><strong>Results: </strong>miR-30c-2-p is underexpressed in RCC cells. Overexpression of miR-30c-2-p promotes apoptosis and inhibits proliferation of ACHN, Caki-1, and 786-O cells. miR-30c-2-3p targets TOP2A, which is elevated in RCC tissues and cells, whereas TOP2A silencing inhibits the proliferation ability of RCC cells. The miRNA-30c-2-3p inhibitor compromises TOP2A shRNA-induced apoptosis of RCC. RCC cells cotransfected with miRNA-30c-2-3p inhibitors and TOP2A shRNAs have a higher proliferation rate than those transfected with only TOP2A shRNAs.</p><p><strong>Conclusions: </strong>Collectively, our results verify that miRNA-30c-2-3p has a tumor suppressor property. miRNA-30c-2-3p inhibits the proliferation of RCC through regulation of TOP2A. The data provide a viable therapeutic target for RCC.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"124-135"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between inflammatory biomarkers and macular pigment optical density in hypertensive patients: a cross-sectional study.","authors":"Chunchuree Kongmeesuk Kaneko,&nbsp;Katsunori Kaneko,&nbsp;Vitoon Jularattanaporn,&nbsp;Thamthiwat Nararatwanchai","doi":"10.2478/abm-2023-0054","DOIUrl":"10.2478/abm-2023-0054","url":null,"abstract":"<p><strong>Background: </strong>Inflammation may be associated with macular pigment optical density (MPOD) degradation.</p><p><strong>Objectives: </strong>The relationship between inflammation and MPOD is evaluated using inflammatory biomarkers, including high sensitivity C-reactive protein (hsCRP), lipid level and ratio, waist circumference (WC), and body mass index (BMI).</p><p><strong>Method: </strong>In this cross-sectional design, 62 hypertensive patients were recruited between January 6 and January 8, 2022, at a primary care unit. The MPOD was measured using the Macular pigment screener II. Blood tests for hsCRP, lipid profile, WC measurement, BMI calculation, and completing a questionnaire were conducted, and statistical analysis was done by using Microsoft Excel 2019 and Stata version 16.1. Spearman's rank correlation test was used to evaluate correlations. Multivariate analysis for adjusting confounders was done by logistic regression.</p><p><strong>Result: </strong>There was a significant negative correlation between hsCRP >3 and MPOD (<i>r</i> = -0.26, <i>P</i> = 0.04).</p><p><strong>Conclusion: </strong>Inflammation was linked to MPOD. Anti-inflammatory agents may be beneficial in preventing MPOD degradation.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"144-149"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection rate among close contacts of patients with coronavirus disease in Japan: a descriptive study and literature review.","authors":"Osamu Yamamura,&nbsp;Hidenori Onishi,&nbsp;Ippei Sakamaki,&nbsp;Ryousuke Fujita,&nbsp;Hirofumi Miyashita,&nbsp;Hiromichi Iwasaki","doi":"10.2478/abm-2023-0051","DOIUrl":"10.2478/abm-2023-0051","url":null,"abstract":"<p><strong>Background: </strong>In Japan, on April 20, 2020, the definition of a close contact regarding coronavirus disease (COVID-19) was changed from a long-term contact time to a specified contact time of 15 min and from a contact distance of 2 m to 1 m.</p><p><strong>Objectives: </strong>We aimed to determine the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection rate among close contacts of patients with COVID-19 and determine the impact of the infection on transmission among close contacts.</p><p><strong>Methods: </strong>The numbers of SARS-CoV-2 tests, SARS-CoV-2-positive cases, and close contacts of patients with COVID-19 were assessed between March 2020 and February 2021 in Fukui Prefecture, Japan. The study period was subdivided into 3 periods. The second and third period contained data with the changed definition of close contact.</p><p><strong>Results: </strong>Overall, 32,238 SARS-CoV-2 tests were performed. There were 545 patients with COVID-19 and 1487 close contacts, of whom 267 tested positive. The highest infection rate occurred in period 3. Distance, protective measures, and contact time with COVID-19 patients influenced the increased infection rate. The infection rate showed a rising trend from 11.1% in period 1 to 19.2% and 20.0% in periods 2 and 3, respectively (Cochran-Armitage test; <i>P</i> < 0.004). Multivariate analysis revealed that female sex was an independent risk factor for infection of close contacts (odds ratio: 2.23; 95% confidence interval: 1.700-2.930).</p><p><strong>Conclusions: </strong>Female sex is a risk factor for transmission by close contacts. The rate of infection among close contacts may be associated with contact time, contact distance, and protective measures.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"115-123"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}