Bangladesh journal of microbiology最新文献

{"title":"Prevalence of Disease and Antibiotic Sensitivity Profile of Hospital Associated Pathogens: A study on a local diagnostic Centre in Dhaka city","authors":"Sharmina Ahmed, R. Islam, S. Ahsan","doi":"10.3329/bjm.v36i2.45535","DOIUrl":"https://doi.org/10.3329/bjm.v36i2.45535","url":null,"abstract":"Dhaka is the capital city of Bangladesh. It is one of the biggest and densely populated cities in the world with a population of 20.2 million people in the Greater Dhaka Area1.While urban life offers many amenities, urban areas can also ponder health hazards to its inhabitants. Urban populations in both lowand highincome countries disclose some of the world’s most noticeable health inequalities.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"99-104"},"PeriodicalIF":0.0,"publicationDate":"2020-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44336615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and partial characterization of organophosphate pesticide degrading bacteria from soil sample of Noakhali, Bangladesh","authors":"Mahin Reza, J. Fiza, F. Hossen, F. Ahmed","doi":"10.3329/bjm.v36i1.44263","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44263","url":null,"abstract":"As the demand for agricultural crops increases, so unsurprisingly does the need for pesticides. Bangladesh is no exception in its excessive and recurrent use of pesticides including malathion1. Malathion, S-(1,2-dicarbethoxyethyl)-O, O-dimethyl dithiophosphate, also known as carbophos, maldison and mercaptothion is a nonsystemic, wide-spectrum organophosphate insecticide used extensively to control a wide range of chewing and sucking insects in paddy fields of Bangladesh2. It is classified as a toxicity class III pesticide with a maximum permitted amount of 8 parts per million (ppm) of malathion to be present as a residue in specific crops used as foods by the U.S. Environmental Protection Agency, EPA3.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjm.v36i1.44263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48010261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Technologies for Food Safety: High Pressure Processing (HPP) and Cold Plasma Technology (CPT) for Decontamination of Foods","authors":"F. Feroz, Showshan Nafisa, R. Noor","doi":"10.3329/bjm.v36i1.44281","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44281","url":null,"abstract":"The food and beverage manufacturing industry represents one of the largest manufacturing sectors worldwide.In a consumerdriven market, food processors are constantly challenged to develop food products with consumer-desired characteristics at affordable costs without compromising food safety. Consumers’ health and wellness-oriented lifestyles lead to a preference for mildly processed, fresher-tasting foods with minimal or no preservatives.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjm.v36i1.44281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45530366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of New Delhi Metallo b Lactamase gene in Uropathogenic E. coli","authors":"S. Ahsan, Anindita Bhowmik, Sharmistha Goswami, N. Uddin","doi":"10.3329/bjm.v36i1.44280","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44280","url":null,"abstract":"The rapid dissemination of antibiotic resistant E. coli is now a worldwide problem. In this study, a total of twenty E. coli obtained from stool were selected to determine resistance to beta lactam antibiotics. Beta–Lactamase are enzymes produced by bacteria that provide multi resistance to beta lactam antibiotics such as penicillin, cephalosporin, cephamycin and carbapenems. Of these isolates (n = 20), 35% were found resistant to Amoxicillin Clavulanate, 5% to Imipenem, 50% to Ceftriaxone and 75% to Ampicillin. PCR amplification confirmed the presence of the New Delhi beta-lactamase gene (blaNDM) in one isolate (5%, n=20). Plasmids of variable sizes were found in all the isolates. Beta lactam antibiotics are now commonly used for the treatment of disease. Resistance of 50% of the isolates to Ceftriaxone is alarming as this indicates that an alternative drug may soon need to replace this antibiotic for successful treatment. The finding of this study is also of public health concern as plasmids were found in most isolates and these mobile genetic elements can be transferred among clinical bacteria, thereby disseminating antibiotic resistance further limiting treatment options. \u0000Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 29-33","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41397742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-reacting surface proteins between different Escherichia coli O157:H7 strains and their immune responses in animal models","authors":"A. Nabi, F. Chowdhury, Z. Jahan, M. Sarkar, F. Rabbi, C. R. Ahsan","doi":"10.3329/bjm.v36i1.44262","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44262","url":null,"abstract":"Enterohemorrhagic Escherichia. coli O157:H7 is one of the significant causes of food and waterborne illness in the developed nations across the globe. Transmission of the disease mainly occurs via the consumption of contaminated food, water and person to persontransmission1. Clinical spectrum of the disease includes watery diarrhea, bloody diarrhea, hemorrhagic colitis and life-threatening hemolytic uremic syndrome (HUS), which may develop in the people of all age groups.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46181663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Methicillin and Vancomycin resistant Staphylococcus aureus on the touch screen of automated teller machines in Dhaka city.","authors":"M. Acharjee, T. Akter, N. Tabassum, M. Rahaman, R. Noor","doi":"10.3329/bjm.v36i1.44279","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44279","url":null,"abstract":"In today’s world, the emerging infectious diseases are the major threat to human health and cause serious fatal outcome in both local and global perspective1,2. The proliferation of pathogenic microorganisms including bacteria, virus, fungi and parasites into the human body through contaminated hand, food, water and dust particles may cause diseases like diarrhea and dysentery 3-5. The use of electronic device such as taps, ATM machine, computer, telephones etc. are increasingly being used to reduce the work load and time 6,7. In public health point of view the surface of these devices may act as one of the major and common routes of pathogenic microbes by which they can easily spread8,9. Several studies have already been conducted throughout the world on the presence of pathogenic contamination on the touch screen of electronic devices like ATM booth, public telephone booths, keypad of mobile phones and computers 3,9. As described in early studies the mobile phones, computer keyboards and automated teller machines (ATM) are now becoming the source of infection because of the vast dermal contact with the key panel10-13. Previous study suggested that most noteworthy pathogens such as Staphylococcus epidermidis, Staphylococcus aureus (MRSA), Staphylococcus alpha haemolyticus, Enterococcus faecalis, Bacillus subtilis, coliforms, Pseudomonas aeruginosa, Escherichia coli and Acinetobacter calcoaceticus have been found from the surface of different electronic devices 14. The probability of dissemination of such microbial contaminates is very high in densely populated country like Bangladesh where the major community doesn’t maintain the personal hygiene and sanitation procedure8. Therefore, increased use of Automated Electronic devices by the people who are not aware of the sanitation and hygiene may pose potential threat to public health. 15-19. Considering all the facts, this study attempted to find out the frequency of multidrug resistant strains (MDR) as well as methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Staphylococcus aureus (VRSA) on the touch screen of automated teller machines (ATMs) of different banks.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"23-27"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjm.v36i1.44279","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44914702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Rhizobium spp. isolated from soil samples of Bangladesh","authors":"Tauhidul Islam Tanim, M. Chowdhury, L. Bari, M. Rahaman, S. R. Rahman, Majibur Rahman","doi":"10.3329/bjm.v36i1.44261","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44261","url":null,"abstract":"Rhizobia are Gram-negative soil-inhabiting bacteria commonly found in nodules of leguminous plants. These bacteria exclusively fix atmospheric nitrogen into ammonia, which is convertible to urea to serve natural fertilizer to the plants. However, rhizobial microbiota of Bangladesh have been studied poorly. Here, twenty isolates were identified as Rhizobium through observation of cultural characteristics, morphological characteristics and different biochemical tests. Isolates differed from one another in terms of their stress response characteristics like salt-stress tolerance,temperature tolerance, antibiotic susceptibility, and pH. Most of the isolates were resistant to ampicillin and erythromycin and streptomycin; and were sensitive to kanamycin and ciprofloxacin. Isolates were clustered into three genotypic groups according to the banding patterns of Amplified Ribosomal DNA Restriction Analysis (ARDRA). All the strains in ARDRA group 1 were Rhizobium azibense, and the others were Rhizobium bangladeshense and Rhizobium qilianshanense. Future studies would validate their capacity of nitrogen fixation and the scope of improvement of these strains to increase their efficiency of nitrogen fixation. \u0000Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 07-10","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjm.v36i1.44261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47455479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary characterization and inhibitory activity of bacteriocin like substances from Lactobacillus casei against multi-drug resistant bacteria","authors":"F. Hasan, Mahin Reza, H. Masud, K. Uddin, M. Uddin","doi":"10.3329/bjm.v36i1.44259","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44259","url":null,"abstract":"Antimicrobial resistance is a mounting threat to the control of infectious diseases worldwide. Due to the increasing rate of drug resistance, there is continuous requirement for new and safe antimicrobial agents both for therapeutic purposes and food industries. The potential of bacteriocin has attracted much attention in recent years. We characterized bacteriocin like substances produced by Lactobacillus casei. This bacterium produces bacteriocin like substances that have the ability to inhibit multi-drug resistant pathogenic bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, and Proteus vulgaris. To investigate the antimicrobial property of the bacteriocin like substances produced by L.casei, cell free supernatant (CFS) was prepared and antagonistic activity of cell free supernatant was determined by agar well diffusion method. Production of bacteriocin like substances was greatly affected by nutrient media, pH, temperature and incubation period. Highest inhibitory activity was observed when L.casei was grown in MRS broth (De Man, Rogosa and Sharpe broth) (pH 4.0) for 72 hoursat 37°C. The active components of CFS responsible for antagonistic activity are proteinaceous in nature since they are susceptible to heat treatment. Partial purification of this CFS was done by organic solvent extraction method. Among the eight isolates tested, only S. auereus, Proteus vulgaris and K. pneumoniae showed more sensitivity to the partially purified crude bacteriocin like substances (CBLS) than CFS.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49293057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Microbiological Quality of Fresh Juices Vended in Dhaka City","authors":"Kayseri Afroz, Tahmina Shammi, S. Kabir","doi":"10.3329/bjm.v36i1.44283","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44283","url":null,"abstract":"Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. \u0000Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42426873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Status of Street Foods in Different University Premises of Dhaka City, Bangladesh","authors":"Nafisa Tanjia, N. Akhter, Ajoy K. Roy, Mir Shefaly Akhter, Muniruddin Ahmed, S. Islam","doi":"10.3329/bjm.v36i1.44282","DOIUrl":"https://doi.org/10.3329/bjm.v36i1.44282","url":null,"abstract":"we identified pathogenic organisms in street foods sold around the University premises 1. Street foods are usually vended in stalls, roadside stands, different types of push-carts, hawker with loads. A large number of people prefer these foods because of the accessibility, reasonable cost, good taste and to some extent for its nutritional value 2, 3. However, these foods can be contaminated from the raw materials to the storage and distribution4. Different studies highlighted the potential role of food borne illnesses due to consumption of street foods 5-9. Also the organisms present in different types of street food samples were identified as Multidrug resistant 10. Food spoilage is another reason of contamination because of its long exposure on roadsides while vending 2, 3.","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"36 1","pages":"45-47"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43808071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}