Bangladesh journal of microbiology最新文献

{"title":"Textile Dye Decolorization Potential of Bacillus pumilus and Staphylococcus saprophyticus Isolated From Textile Industry Effluents","authors":"Nayeema Talukder Ema, Zannatul Mayua, Imtiaj Uddin Bhuyian, Anowara Begum, Humaira Akther, Sangita Ahmed","doi":"10.3329/bjm.v40i2.73318","DOIUrl":"https://doi.org/10.3329/bjm.v40i2.73318","url":null,"abstract":"Textile dyeing industries are usually chastised for being big polluters due to the poisonous nature of most dyes, which endangers all kinds of life, including people. In this study, dye degrading bacteria were isolated from water and soil samples contaminated with textile dye taken from Batik palli in Narayanganj and the ability of five isolated bacteria including Staphylococcus saprophyticus, Bacillus pumilus, Micrococcus endophyticus, Pseudomonas mendocina, and Acinetobacter baumannii, to decolorize crystal violet (CV) dye was investigated. Among these, Bacillus pumilus decolorized 58% of CV at 250 ppm, while Staphylococcus saprophyticus decolorized 48% of CV, after 3 days of incubation at 37°C. We examined multiple temperature and pH conditions to determine the best parameters for CV dye decolorization. Bacillus pumilus boosted decolorization rates by 65.39% at 37°C and at pH 7.0, while Staphylococcus saprophyticus increased decolorization rates by 58.73%, at 37°C and at pH 5.0. Furthermore, extending the incubation period to 6 days enhanced decolorization rate in both isolates, with Bacillus pumilus increased from 58% to 65% and Staphylococcus saprophyticus increased from 48% to 58%. Nevertheless, the inclusion of co-substrates such glucose and yeast extract further boosting decolorization rate for both isolates, approximately tripling it. As a result, this study discovered indigenous bacteria capable of decolorizing CV dye, implying that they could be employed in the treatment of textile wastewater effluents.\u0000Bangladesh J Microbiol, Volume 40, Number 2, December 2023, pp 50-54","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"120 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141360802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Thermoacidophiles from Yellowstone Hot Spring","authors":"Tajul Islam, R. Ceballos, Lise Øvreås","doi":"10.3329/bjm.v40i2.73798","DOIUrl":"https://doi.org/10.3329/bjm.v40i2.73798","url":null,"abstract":"Aerobic methanotrophic bacteria maintain an unrivalled capability of utilizing methane as their sole carbon and energy source and have been retrieved from various environments. Phylogenetically, true aerobic thermoacidophilic methane oxidizers capable of growing below pH 3 have hitherto been associated only with the phylum Verrucomicrobia. In this report, the initial detection of a moderately thermoacidophilic Methylococcus-like Type Ib methanotroph of the class Gammaproteobacteria from an acidic thermal spring (50oC and pH 2.8) in the Yellowstone National Park, USA is presented. The isolate, termed YT-MC, was identified in a methane enrichment (55°C), which may represent a novel strain in the family Methylococcaceae Type Ib. The existence of this bacterium in the enrichments was demonstrated by the detection of pmoA gene, Southern blotting technique, phase-contrast, and electron microscopy. The coccus-typed cells showed tubular membranes instead of intracytoplasmic membrane systems (ICM). The soluble methane monooxygenase (sMMO) was not detected by PCR, indicating that the biotransformation of methane to methanol is oxidized by the particulate methane monooxygenase (pMMO). Moreover, YT-MC performs in a formerly undiscovered active biological methane sink in geothermal acidic environments, magnifying our knowledge of its ecological role in methane cycling, diversity, and coexistence of aerobic methanotrophy. Furthermore, the present study also reports the isolation and identification of an alphaproteobacterial heterotroph (strain YT-AC) and a verrucomicrobial methanotroph (strain YT-VM) from the same environment. \u0000Bangladesh J Microbiol, Volume 40, Number 2, December 2023, pp 86-94","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"118 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141360998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of Plant Growth Promoters (PGP) Isolated from Agricultural Fields of Bangladesh","authors":"Halima Habib, Shammi Akhter Trishna, A. Begum, H. Akhter","doi":"10.3329/bjm.v40i2.73794","DOIUrl":"https://doi.org/10.3329/bjm.v40i2.73794","url":null,"abstract":"With an emphasis on leguminous plants like Glycine max (soybean), Clitoria ternatea, and non-leguminous plants like Phaseolus lunatus, this study investigates the diversity of Plant Growth-Promoting Rhizobacteria (PGPR) isolated from the root nodules and rhizospheres of agricultural soil. Seventeen of the 42 isolates were chosen for further analysis. Isolates were screened through phenotypic characterization, presumptive identification, and evaluation of their plant growth-promoting (PGP) qualities. Amplification of the nitrogen[1]fixing nifH gene, the nodulation-inducing nodC gene, and the 16S rRNA were among the molecular investigations carried out. Three examined PGP features were present in 35% of the isolates, according to the results. From pH levels of 4.5 to 9, temperatures of 37oC to 40oC and salt concentrations of 1% or less, the isolates showed versatility in a variety of environmental circumstances. Resistant to high temperatures (40°C), slow-growing rhizobia (22%) were shown to be sensitive to high pH (9). Tilt and Tafgor pesticides had a more severe influence on PGP traits and development than Shadhin G and Semcup. The design and efficacy of biofertilizers, agro-economic progress, and sustainable agriculture are among the topics on which this qualitative study sheds important light. \u0000Bangladesh J Microbiol, Volume 40, Number 2,  December 2023, pp 66-74","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"113 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141362972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Potential Azo dye Degrading/Tolerating Bacteria from Polluted Environment","authors":"Monira Mehzabin, S. Ahsan","doi":"10.3329/bjm.v40i2.73534","DOIUrl":"https://doi.org/10.3329/bjm.v40i2.73534","url":null,"abstract":"Various industries including the food and textile industries result in the release of azo dyes into the environment. These dyes are known to exert toxic effects on humans, animal and plants. Biological methods of azo dye bioremediation is a solution for the detoxification of azo dye in the environment. The present study was undertaken to isolate bacteria with potential for azo dye bioremediation. From five polluted environment samples, eight bacteria were isolated in azo dye supplemented Nutrient agar. Six isolates (75%, n=8) decolorized azo dye completely following 24 hours of incubation. The control tube with no bacterial inoculation remained blue, indicating that only microbial biotransformation processes were taking place. In each case, a blue-colored ring remained at the top, indicating the anoxic nature of the dye decolorization process. All isolates grew in presence of 400 ppm, 60% tolerated 600 ppm and 20% tolerated 800 ppm, whereas 1000ppm was inhibitory for growth of all isolates. Dead autoclaved cells of three representative isolates were tested for biosorption potential. Only one isolate turned the methylene blue supplemented nutrient broth colorless. This explains that this isolate was not metabolizing methylene blue rather it bound the dye to the cell structure. Two isolates did not show biosorption abilities indicating that their mechanism of bioremediation was enzymatic reduction. 16s rDNA sequencing identified two of the isolates as Lysinibacillus capsici and Stenotrophomonas muris.\u0000Bangladesh J Microbiol, Volume 40, Number 2, December 2023, pp 56-59","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" 589","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141364159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and Membrane Stabilization Activity of Plant Extracts Against Pathogenic Bacteria and Fungi","authors":"Nadia Islam Tumpa, Md Shafiqur Rahman, M. A. Manchur","doi":"10.3329/bjm.v40i2.73797","DOIUrl":"https://doi.org/10.3329/bjm.v40i2.73797","url":null,"abstract":"The use and search for antimicrobial drugs derived from plants have accelerated in recent years. The present study was aimed to determine the antimicrobial, antibiofilm and membrane stabilization activities of plant extracts. Crude extracts of four plants namely, Mikania scandens (L.), Mimosa pudica (L.), Murraya paniculata (L.), and Syzygium aromaticum (L.) were tested against eleven human pathogens including four biofilm producing bacterial strains Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus, and one fungal strain Candida albicans. The antimicrobial activities as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts were evaluated using disc diffusion and macro-broth dilution methods respectively. Among the plant extracts, ethanol extract of S. aromaticum exhibited the largest zone of inhibition 35 mm in diameter against Bacillus subtilis at 500 µg/disc concentrations. The lowest MIC (1000 µg/ml) and MBC (2500 µg/ml) were determined against P. aeruginosa with the same extract of S. aromaticum. In case of time kill assay, S. aromaticum extract showed the lowest optical density (OD600) 0.2 against E coli in 3h at MBC concentration. Moreover, the same extract of S. aromaticum displayed the strong antibiofilm activity, inhibiting 100% biofilm formation of E. coli at 2×MIC concentration. Furthermore, in vitro experiment was performed to evaluate membrane stabilization activity of plant extracts and S. aromaticum showed 100% activity in stabilizing cell membranes, preventing hemolysis of red blood cells. Therefore, this study provides valuable insight for designing antimicrobial products to efficiently eliminating human infections, and the plant extracts could be the potential antimicrobial agent.\u0000Bangladesh J Microbiol, Volume 40, Number 2, December 2023, pp 76-85","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"104 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141361301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico Evolutionary Insights into Prokaryotic and Eukaryotic Malate Dehydrogenases (MDH) Support the Archaebacterial Origin of Eukaryotes","authors":"Israt Dilruba Mishu, Nowshin Kabir, Zimam Mahmud","doi":"10.3329/bjm.v40i1.71706","DOIUrl":"https://doi.org/10.3329/bjm.v40i1.71706","url":null,"abstract":"Malate Dehydrogenase (MDH) stands as a pivotal enzyme crucial for cellular energy metabolism, orchestrating the conversion of malate to oxaloacetate in both prokaryotic and eukaryotic organisms. This study delves into the evolutionary trajectories of MDH1 (cytoplasmic) and MDH2 (mitochondrial), offering substantial evidence supporting the archaebacterial origin of eukaryotes. The dataset spans nine groups, encompassing human MDH1 and MDH2, mammalian MDH1 and MDH2, amphibian MDH2, arthropod MDH2, amoeba MDH1, archaea, and bacteria. Protein BLAST analysis revealed significant sequence homology in mammalian MDH1, particularly among primates, underlining a close evolutionary connection. Conversely, lower eukaryotes, including amoeba, arthropods, and amphibians, exhibited marked divergence from human MDH1 and MDH2. Phylogenetic analysis unveils distinct clusters for MDH1 and MDH2, accentuating significant genetic diversity between mitochondrial and cytoplasmic MDH enzymes. Prokaryotic MDH sequences cluster with human mitochondrial MDH2, while MDH1 forms a separate cluster. Staphylococcus MDH aligns with archaeal MDH, emphasizing the diversity within bacterial MDH evolution. Protein variability analysis indicates noteworthy divergence of human MDH1 from prokaryotic MDH, while MDH2 displays comparatively lower divergence. Pairwise evolutionary divergence analysis sheds light on complex relationships among MDH protein sequences. Human MDH1 shows close evolutionary ties to mammalian MDH1, whereas MDH2 exhibits a unique pattern, aligning closely with mammalian MDH2, arthropods, and amphibians. Furthermore, MDH2 demonstrates closer proximity to bacterial MDH, supporting a bacterial origin of mitochondrial MDH. In contrast, MDH1 displays less divergence to archaeal MDH than its bacterial counterpart, endorsing an archaeal origin for cytoplasmic MDH. In conclusion, this study provides compelling support for the archaebacterial origin of eukaryotes, suggesting a bacterial endosymbiont within an archaeal host that evolved into mitochondria. It contributes valuable insights into MDH evolution, unraveling the intricate relationships and unique adaptations shaping the evolutionary history of eukaryotic cells. \u0000Bangladesh J Microbiol, Volume 40, Number 1, June 2023, pp 15-24","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Assessment and Detection of Legionella species in the Water System of A Luxury Hotel in Dhaka city, Bangladesh","authors":"Nazmun Naher, Md. Latiful Bari, Sangita Ahmed","doi":"10.3329/bjm.v40i1.71711","DOIUrl":"https://doi.org/10.3329/bjm.v40i1.71711","url":null,"abstract":"Legionella cause Legionnaires’ disease and Pontiac fever, and, more rarely, extrapulmonary infections, collectively referred to as legionellosis. Legionella pneumophila causes serious pneumonic infections, and water and Air Conditioning systems are a common source. We performed this study focusing on identifying and assessing the potential sources of Legionella spp. in a luxury hotel water system in Dhaka, Bangladesh. As hotels often have complex water distribution systems that can harbor and facilitate the growth of these bacteria, understanding the risks and implementing effective control measures is crucial for public health. This study determines the presence of Legionella spp. and potential factors contributing to their proliferation within the hotel water systems. In total 42 water samples and six swabs from 7 different points were collected multiple times between March 2018 and April 2019 and were analyzed. Legionella spp. was detected in all samples (100%) tested. The highest colony counts were observed in water samples from the hot water tank and cooling system. The findings highlight the importance of regular monitoring, maintenance, disinfection, and flushing protocols to mitigate the risk of Legionella spp., contamination, and subsequent health issues among hotel guests and staff. \u0000Bangladesh J Microbiol, Volume 40, Number 1, June 2023, pp 33-39","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"11 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug Resistance Pattern of Pseudomonas aeruginosa Isolated from Patients with Nosocomial Infection","authors":"Poulomi Saha, Moumita Chakrabarty, Rubaiya Binte Kabir, C. R. Ahsan, M. Yasmin","doi":"10.3329/bjm.v40i1.71687","DOIUrl":"https://doi.org/10.3329/bjm.v40i1.71687","url":null,"abstract":"Multidrug resistant (MDR) Pseudomonas aeruginosa is a threat to the patients having nosocomial infections as this pathogen increases the inpatients’ morbidity and mortality by slowing down the whole treatment process. The aim of this study was to evaluate multidrug resistant phenotypes among nosocomial strains of P. aeruginosa for analyzing their antibiotic susceptibility pattern. A total of 108 P. aeruginosa clinical isolates were recovered from various samples (pus, wound swab, urine, sputum, blood and tracheal aspirate) of patients with nosocomial infections. Antibiogram was performed by disc diffusion according to Kirby-Bauer method to study the antibiotic sensitivity pattern of the pathogen against 14 regularly used antibiotics (amikacin, aztreonam, ceftazidime, ceftriaxone, co-trimoxazole, ciprofloxacin, gentamicin, levofloxacin, meropenem, netilmicin, doxycycline, amoxicillin/clavulanic acid, piperacillin/tazobactam, and tigecycline) in Bangladesh. The overall frequency of drug resistance was found to be very high (53.7% - 98.1%) to all of the anti-pseudomonal drugs tested. Resistance of P. aeruginosa strains against piperacillin-tazobactam was significantly less (53.7%) as compared to other thirteen antibiotics. However, isolates showed highest resistance (98.1%) to aztreonam. Next in order of resistance were doxycycline (95.4%), ceftriaxone (94.4%), amoxiclav (93.5%), and the others. The present study suggests that regularly used medications can no longer be utilized as first line therapies for suspected pseudomonad infections. This study claims for urgent epidemiological monitoring of the MDR P. aeruginosa strains in all hospitals of Bangladesh to prevent rapid dissemination of this opportunistic pathogen. \u0000Bangladesh J Microbiol, Volume 40, Number 1, June 2023, pp 7-13","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"1999 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140246338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Identification of Chromium Resistant Bacteria from Poultry Excreta","authors":"Md. Abu Sayem Khan, Md Muzahidul Islam Badhon, Supantha Rivu, S. R. Rahman","doi":"10.3329/bjm.v40i1.71593","DOIUrl":"https://doi.org/10.3329/bjm.v40i1.71593","url":null,"abstract":"The occurrence of heavy metal-resistant bacteria in the environment acts as an indicator of heavy metal pollution. The impact of heavy metal contamination in co-selection and proliferation of antimicrobial[1]resistant bacteria is well-documented in existing literature. The present study aims to determine the occurrence of chromium-resistant bacteria (CRB) in poultry feces and to characterize their antimicrobial resistance and biofilm formation ability. 28 CRBs were isolated by inoculating samples on chromium-amended media. 6 of 28 isolates were resistant to a maximum of 1000 ppm chromium. Isolated CRBs also showed varying degrees of resistance to other heavy metals, including Cd, Ni, and Hg. Biofilm formation was observed in 67.8% isolates, of which 60.7% and 7.1% were weak and moderate biofilm former, respectively. All the isolates exhibited sensitivity to Gentamicin, Chloramphenicol, Imipenem, and Amikacin. Only one isolate was multi-drug resistant. In addition, all the isolates possessed chromate reductase gene that confirmed their chromium-reducing activity. Two isolates were identified as Bacillus altituidinis and Brevibcillus parabrevis on the basis of 16s rRNA analysis. Further estimation of chromium-reduction capacity and whole genome analysis will reveal their bioremediation potential. \u0000Bangladesh J Microbiol, Volume 40, Number 1, June 2023, pp 1-6","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"11 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between antibiotic resistance and biofilm formation in Klebsiella spp.","authors":"Dipan Barai, Mohibur Rahman Rafe, Najmun Nahar, S. Begum, Humaira Akther, Anowara Begum","doi":"10.3329/bjm.v40i1.71709","DOIUrl":"https://doi.org/10.3329/bjm.v40i1.71709","url":null,"abstract":"Antibiotic resistance among various microbial species is becoming a global threat to human health. MDR, or multidrug resistance, is an organism’s ability to tolerate the effects of many antimicrobial treatments. Klebsiella spp. is the major bacteria that causes a variety of illnesses, including urinary tract infections. The primary goal of this study is to look into the relationship between antibiotic resistance and Klebsiella spp. biofilm production. This study included 26 clinical isolates of Klebsiella spp. (n=26) collected from a tertiary hospital in Bangladesh. Isolates were obtained from blood, wound swabs, urine, and other sources. Following isolation and identification, ampicillin resistance was found in all MDR Klebsiella spp. isolates, followed by Cefotaxime (84.61%), Polymyxin B (84.61%), Amoxicillin (80.76%), Trimethoprim (69.23%), Doripenem (57.69%), Ciprofloxacin (57.69%), Imipenem (50.0%), Meropenem (38.46%), and Oxytetracycline (34.61%). Out of 26 clinical isolates, about 26.92% (n=7) were positive for the bla-NDM-1 gene. However, 15.8% of Klebsiella spp. (n = 4) isolates tested positive for the blaOXA-1 gene. There were no bla-KPC gene positives. Plasmid profile analysis revealed that 24 of 26 Klebsiella spp. isolates included numerous plasmids ranging in size from less than 2kb to more than 10kb. Biofilm formation found that 31% of samples were extremely positive for biofilm formation, 69% were medium biofilm formation, and (n=4) isolates were positive for biofilm resistance gene out of a total of 26 isolates. This study provides an early report on the widespread presence of carbapenem-resistant Klebsiella spp., demonstrating the need for intensive surveillance systems and research initiatives in Bangladesh to reflect the influence of multidrug resistance features in clinical isolates and their risks. \u0000Bangladesh J Microbiol, Volume 40, Number 1, June 2023, pp 25-32","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"27 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}