Bangladesh journal of microbiology最新文献_第7页

Potential Drug Target Identification of Legionella pneumophila by Subtractive Genome Analysis: An In Silico Approach 应用消减基因组分析法鉴定嗜肺军团菌的潜在药物靶点

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42638

Sadikur Rahman Shuvo, S. Shakil, F. Ahmed

{"title":"Potential Drug Target Identification of Legionella pneumophila by Subtractive Genome Analysis: An In Silico Approach","authors":"Sadikur Rahman Shuvo, S. Shakil, F. Ahmed","doi":"10.3329/BJM.V35I2.42638","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42638","url":null,"abstract":"Though Legionella pneumophila is an opportunistic pathogen, recent reports about multi drug resistance in L. pneumophila is alarming. Annotated whole genome provides a pool of information which is applied for therapeutic drug targets identification in pathogenic bacteria. Subtractive genomic analysis is a pragmatic approach to screen the essential proteins present in pathogen but absent in host. Phylogenetically closely related L. pneumophila str. Philadelphia and L. pneumophila str. ATCC43209 protein profiles were analyzed to identify putative drug targets. Paralogous duplicate profiles were primarily discarded using CD-hit suit. Six hundred and ninety one L. pneumophila str. Philadelphia and 690 L. pneumophila str. ATCC43209 human homologous proteins were excluded using blstP. Among the human non-homologous proteins, the essential proteins for bacteria were separated using DEG tool. For both strains, one hundred and nineteen essential proteins were marked which participate in various metabolic pathways. Among them 11 unique proteins were found. Beside it, 15 and 16 exposed surface proteins were present in strain Philadelphia and ATCC43209 respectively. These unique and cell surface proteins can be utilized for effective drug and vaccine targets. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 102-107","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJM.V35I2.42638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Isolation and Identification of Pesticides Degrading Bacteria from Farmland Soil 农田土壤中农药降解菌的分离与鉴定

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42635

Atikur Rahman, A. Arefin, Otun Saha, M. Rahaman

引用次数: 6

Influence of multi-species biofilm formed in vitro from different environmental samples on the drug-resistance traits of resident bacteria 不同环境样品体外形成的多菌种生物膜对驻留菌耐药特性的影响

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42639

S. K. Munshi, Tamanna Haque, R. Noor

引用次数: 6

Microbiological safety of commonly available eye ointments and their in-vitro anti-bacterial potency 常用眼膏的微生物安全性及其体外抗菌效力

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42641

M. Acharjee, Rakhi Baishnaib, R. Noor

{"title":"Microbiological safety of commonly available eye ointments and their in-vitro anti-bacterial potency","authors":"M. Acharjee, Rakhi Baishnaib, R. Noor","doi":"10.3329/BJM.V35I2.42641","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42641","url":null,"abstract":"Current study attempted to check out the microbiological quality of some common sterile ointment commonly implemented for the treatment of eye infections in Bangladesh. Seventeen (17) different eye ointment (T-Mycin, Aprocin, Bactin, Optimox and Cloram, Hypomer gel, Sonexa, Polytracin, Cero, Aristobet, Lotepred, Herpigel, Gentob, Xoviral, Zirgan, Xovir, Tomycin, Tobirax, AFm-plus and Parafresh) were microbiologically examined through common and traditional cultural methods. Total viable bacterial and fungal contamination was found up to 106 and 105 cfu/ml respectively. Among the 17 samples T-Mycin, Aprocin, Sonexa, Polytracinand Tomycinwere free from fungal contamination. All the samples significantly exceeded United States Pharmacopeia (USP) or British Pharmacopeia (BP) limit (<102 cfu/ml) in case of Total viable bacteria and fungus contamination. While the coliforms (Escherichia coli and Klebsheilla spp.) were absent in all samples, the prevalence of Staphylococcus spp. was 100% in all samples up to 103 while the Bacillus spp. was found up to 102 cfu/ml. Pseudomonas spp. was cultivated in TMycin, Aprocin Hypomer gel, Sonexa, Polytracin, Cero, Aristobet and Lotepred up to 103cfu/ml. All the 5 drugs showed their antibacterial potency with satisfactory range of zone diameter againstE. coli, Pseudomonas spp., Staphylococcus spp., Klebsheilla spp. and Bacillus spp. In case of all 17 ointments the average zone diameter range was noticed within 13mm-22mm. The highest zone diameter (22mm) was recorded against E. coli produced by Aprocin and minimum zone diameter (13mm) was recorded against Bacillus spp. produced by Bactin, Optimox and Cloram.Gentamicin and Streptomycin were used as positive control against the tested bacteria. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 122-126","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":"45 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41302056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of microbiological quality and safety of fermented and non-fermented Khmer Rice Noodles in Cambodia 柬埔寨发酵和非发酵高棉米粉的微生物质量和安全性评价

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42634

Ek Sopheap, Y. Inatsu, S. Zaman, B. Buntong

{"title":"Assessment of microbiological quality and safety of fermented and non-fermented Khmer Rice Noodles in Cambodia","authors":"Ek Sopheap, Y. Inatsu, S. Zaman, B. Buntong","doi":"10.3329/BJM.V35I2.42634","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42634","url":null,"abstract":"Fermented and non-fermented Khmer rice noodles is produced basically from indica rice, a popular food item and most important nutrition sources along with an integral part of rural household’s food security of Cambodian population. This study was done to evaluate the microbiological quality of fermented and non-fermented Khmer rice noodle samples sold throughout the country. In total, 75 Khmer rice noodle samples, (23 fermented and 52 nonfermented) were collected from five local varieties (Nom-Banhchok, Nom-Banhhoy, Koyteavkat, Lotchhar and Koyteav) and three locations (Kandal province, Siem Reap province and Phnom Penh city) of Cambodia. The study results showed that irrespective of sample location, all the fermented and non-fermented rice noodle samples analyzed were either contaminated with faecal origin bacteria (Enterococcus spp., and coliform), or faecal indicator bacteria including Escherichia coli and pathogenic bacteria including, Staphylococcus spp., and Bacillus spp. Of the fermented rice noodles about 74% and 98% of non-fermented rice noodles was found contaminated with all the above mentioned bacteria. In addition, 30% samples were found contaminated with Klebsiella pneumonia spp. Furthermore, among the Staphylococcus aureus isolates, three isolates TM-K5-3, OM-L4 and DM-KK3-2 was seen producing protein A (aggregated polysaccharide specific immune latex strongly), positive clumping factor production and harbored type six coagulase gene and among the B. cereus strains, 12 B. cereus, isolates was seen produced enterotoxin, which may cause severe diarrhea and one B. cereus (DM-NB1) isolate was found harbored CRS gene, which may produce emetic toxin, unsafe for human consumption. Therefore, strong regulatory monitoring should be established in order to improve the quality of food to ensure public health. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 82-88","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJM.V35I2.42634","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43494334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Washing on Reducing Bacterial Loads in Common Vegetables Sold in Dhaka City 洗涤对降低达卡市出售的普通蔬菜细菌负荷的影响

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42637

A. Das, Z. Sultana, Ahamedul Kabir, S. Kabir

{"title":"Effect of Washing on Reducing Bacterial Loads in Common Vegetables Sold in Dhaka City","authors":"A. Das, Z. Sultana, Ahamedul Kabir, S. Kabir","doi":"10.3329/BJM.V35I2.42637","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42637","url":null,"abstract":"Vegetables and fruits can be contaminated with microorganisms from different sources during production and processing. Quality of vegetables usually relies on the quality of processing water and sanitizers used to reduce microbial load and increase shelf-life of vegetables. The present study demonstrates the role of washing on reducing bacterial loads in common vegetables sold in Dhaka city. A total of seventy two samples comprising of eighteen types of fresh vegetables were collected. Total heterotrophic bacteria, coliforms and Enterobacteriaceae were determined in 72 samples including potato, tomato, carrot, cucumber, green chilli, bean, eggplant, cauliflower, turnip, radish, capsicum, pumpkin, cabbage, gourd, papaya, bitter gourd, snake gourd and ridge gourd before and after washing with tap and filtered water, halotab water, bleaching powder (0.2%) solution and bleaching powder (0.2%) with citric acid (0.1%) for 10 minutes. Tap water, filtered water, bleaching powder (0.2%), halotab solution and bleaching powder (0.2%) + citric acid (0.1%) solutions were found to be effective and significantly reduced the bacterial loads in vegetables compared to the unwashed vegetables (P<0.01). Effect of washing vegetables using tap and filtered water were found to reduce load of total viable bacteria to 0.85-2.05 log10cfu/g and 2.38-3.36 log10cfu/g, respectively compared to those of unwashed samples. The efficiency of washing for reducing the bacterial load depends on the quality of washing water used and the types of disinfectant solutions used. An appropriate washing technique can significantly reduce the bio burden in contaminated vegetables and may reduce the risk of foodborne infections associated with the consumption of vegetables. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 96-101","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJM.V35I2.42637","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45141364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Microbiological Quality of Commercial Fruit Juices-Current perspectives 商品果汁的微生物质量现状

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42643

T. Ahmed, K. Das, Md. Aftab Uddin

{"title":"The Microbiological Quality of Commercial Fruit Juices-Current perspectives","authors":"T. Ahmed, K. Das, Md. Aftab Uddin","doi":"10.3329/BJM.V35I2.42643","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42643","url":null,"abstract":"The purpose of this review article is to introduce the importance of fruit juices for human health living in the country and in a broad, and to develop awareness among the people about the diseases caused by pathogens associated with fruit juice. Health benefits of juices have been included in this article and how the same juice can cause problems among people of different ages have been discussed. Contamination sources and the ways to prevent them is very important issue in protecting public health Some future recommendations for fruit juices have also been added in this article. Different diseases caused by various microbial agents and the associated symptoms after consuming contaminated fruit juices worldwide are discussed in this review. This review was aimed at the possible sources of microbial contamination, disease caused by them and determining some ways to avoid such phenomena. From the information provided here, it was noticeable that commercial fruit juice can also harbor pathogenic microorganisms which can cause serious disease outbreaks. The contamination can also be initiated during in house consumption if lack of awareness prevails among the consumers. Manufacturing process should be much more strict in this regard to assure the public health safety. Commercially available fruit juices are consumed worldwide among different ages of people and if not processed properly, this healthy drink may be hazardous for human health. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 128-133","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJM.V35I2.42643","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43847370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Seasonal variation of bacterial load in river and lake waters of Dhaka city, Bangladesh 孟加拉国达卡市河流和湖水细菌负荷的季节变化

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42640

Suprio Sadat Sikdar, M. Abony, T. Zerin, A. Banik, Suvamoy Datta

{"title":"Seasonal variation of bacterial load in river and lake waters of Dhaka city, Bangladesh","authors":"Suprio Sadat Sikdar, M. Abony, T. Zerin, A. Banik, Suvamoy Datta","doi":"10.3329/BJM.V35I2.42640","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42640","url":null,"abstract":"Surface water is often contaminated due to human excreta and urban and industrial pollution. The increased population growth and industrialization are likely to exacerbate the situation. Microbiological analysis of waters from rivers (Turag and Buriganga) and lakes (Banani and Dhanmondi) around Dhaka city were conducted for Spring, Monsoon and Fall seasons of the year, 2016 and 2017. Total Viable Count (TVC), Total Coliform Count (TCC), Total Fecal Coliform Count (TFCC) and Total Salmonella Shigella Count (TSSC) were conducted to obtain bacterial load in both enriched and without enrichment water samples. All the Isolates were identified by microscopy, cultural characteristics and biochemical methods. Most of the water reservoirs have a decline in the bacterial load in monsoon where the microbial load of pathogens is highest during spring. Without enrichment, E. coli, Enterobacter sp., Klebsiella sp. and S. aureus were abundant, where others like Shigella sp., Proteus sp., Serratia sp., Pseudomonas sp., Acinetobacter sp., and Alcaligenes faecalis mostly exhibited growth following enrichment. Results indicate that pathogenic environmental isolates can cause serious health issue if water is left untreated or poorly treated from reservoirs within and around Dhaka city. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 116-121","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJM.V35I2.42640","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43191159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Drug Resistance Associated with Blood Borne Bacteria in Dhaka Metropolis 达卡大都市血源性细菌的耐药性

Bangladesh journal of microbiology Pub Date : 2019-08-18 DOI: 10.3329/BJM.V35I2.42642

T. Ahmed, R. Dey, R. Noor

{"title":"Drug Resistance Associated with Blood Borne Bacteria in Dhaka Metropolis","authors":"T. Ahmed, R. Dey, R. Noor","doi":"10.3329/BJM.V35I2.42642","DOIUrl":"https://doi.org/10.3329/BJM.V35I2.42642","url":null,"abstract":"Blood borne infections are easy to contract during hospital procedures and can easily be transferred to other personnel in a healthcare setting. Bacteremia has become a common incidence and treatment is by administration of antibiotics. But this is not a simple task any more, since the pathogenic bacteria causing bacteremia are becoming resistant to a wide range of antibiotics. Blood samples were collected from outdoor patients seeking laboratory tests in Dhaka city. Blood samples were inoculated onto blood agar medium and after incubation for 37oC for 24 hours, bacterial isolates were identified and subjected to antibiotic susceptibility test by following the Kirby-Bauer method. The current present study deals with 100 patients of bacteremia from whom 150 isolates of 15 different bacterial genera have been collected among which the most prominent bacteria were Staphylococcus spp. (51 isolates), Pseudomonas spp. (19 isolates) and Escherichia spp. (19 isolates). After the antibiotic susceptibility test it was found that all isolates were resistant to a number of commonly used antibiotics. Twenty eight different antibiotics were used for this study. All isolates showed resistance to CAR, ATM, TOB, CXM, FD, CL, CAZ, AMC. NET and CN showed the most effective results (can effectively clear 9 and 10 types of isolates respectively found in this study). New discovery of drugs to fight these resistant pathogens is needed. In the meantime, safe administration of drugs, handling patients with appropriate protective personal clothing and apparatus, proper waste disposal managements in the hospital should be maintained strictly. \u0000Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 134-140","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48964641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity of Salmonella enterica Strains Isolated from Sewage Samples of Different Hospitals in Bangladesh 孟加拉不同医院污水样本中分离的肠沙门氏菌菌株的遗传多样性

Bangladesh journal of microbiology Pub Date : 2019-01-15 DOI: 10.3329/bjm.v35i1.39804

T. Arafat, Rasel Mahmud, Tauhidul Islam Tanim, M. Chowdhury, M. Rahaman, S. R. Rahman, Md. Majibur Rahman

{"title":"Genetic Diversity of Salmonella enterica Strains Isolated from Sewage Samples of Different Hospitals in Bangladesh","authors":"T. Arafat, Rasel Mahmud, Tauhidul Islam Tanim, M. Chowdhury, M. Rahaman, S. R. Rahman, Md. Majibur Rahman","doi":"10.3329/bjm.v35i1.39804","DOIUrl":"https://doi.org/10.3329/bjm.v35i1.39804","url":null,"abstract":"The degree of salmonellosis is associated with the genetic diversity of the Salmonella enterica. Here, the genetic diversity of Salmonella enterica isolated from hospital sewage samples of Bangladesh were analyzed to elucidate the prevalence of S. enterica by random amplification of polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA). Twenty six isolates were identified as S. enterica by cultural and biochemical methods as well as 16srDNA sequencing. These isolates showed two types of ARDRA and eight distinct RAPD patterns. All of these isolates possessed invA gene. However, agfA and fliC genes were found in 21 isolates and 16 isolates respectively. All of the isolates were resistant to rifamycin but most of them were sensitive to ceftriaxone and streptomycin. The study indicated that the genetic diversity is very high among the S. enterica isolates of Bangladesh. Therefore, such diversity may contribute to the future outbreak of salmonellosis in Bangladesh. \u0000Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 57-60","PeriodicalId":84251,"journal":{"name":"Bangladesh journal of microbiology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjm.v35i1.39804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44534401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4