Genetic Diversity of Salmonella enterica Strains Isolated from Sewage Samples of Different Hospitals in Bangladesh

Bangladesh journal of microbiology Pub Date : 2019-01-15 DOI:10.3329/bjm.v35i1.39804

T. Arafat, Rasel Mahmud, Tauhidul Islam Tanim, M. Chowdhury, M. Rahaman, S. R. Rahman, Md. Majibur Rahman

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引用次数: 4

Abstract

The degree of salmonellosis is associated with the genetic diversity of the Salmonella enterica. Here, the genetic diversity of Salmonella enterica isolated from hospital sewage samples of Bangladesh were analyzed to elucidate the prevalence of S. enterica by random amplification of polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA). Twenty six isolates were identified as S. enterica by cultural and biochemical methods as well as 16srDNA sequencing. These isolates showed two types of ARDRA and eight distinct RAPD patterns. All of these isolates possessed invA gene. However, agfA and fliC genes were found in 21 isolates and 16 isolates respectively. All of the isolates were resistant to rifamycin but most of them were sensitive to ceftriaxone and streptomycin. The study indicated that the genetic diversity is very high among the S. enterica isolates of Bangladesh. Therefore, such diversity may contribute to the future outbreak of salmonellosis in Bangladesh. Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 57-60

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孟加拉不同医院污水样本中分离的肠沙门氏菌菌株的遗传多样性

沙门氏菌病的程度与肠沙门氏菌的遗传多样性有关。本文采用随机扩增多态性DNA (RAPD)和扩增核糖体DNA限制性内切分析(ARDRA)的方法，对孟加拉国医院污水样本中分离的肠沙门氏菌进行遗传多样性分析，以阐明肠沙门氏菌的流行情况。经培养、生化及16srDNA测序鉴定，26株分离株为肠球菌。这些分离株表现出2种ARDRA类型和8种不同的RAPD模式。所有分离株均具有invA基因。而在21株和16株中分别发现agfA和fliC基因。所有菌株均对利福霉素耐药，但大部分菌株对头孢曲松和链霉素敏感。研究表明，孟加拉国肠球菌分离株具有很高的遗传多样性。因此，这种多样性可能会导致孟加拉国未来暴发沙门氏菌病。孟加拉国微生物学杂志，第35卷第1期2018年6月，第57-60页

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Bangladesh journal of microbiology

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