Annals of Laboratory Medicine最新文献_第6页

Customized Quality Assessment of Healthcare Data. 医疗数据的定制质量评估。

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-17 DOI: 10.3343/alm.2024.0084

Jieun Shin, Jong-Yeup Kim

引用次数: 0

Association Between Clonal Hematopoiesis of Indeterminate Potential and Brain β-Amyloid Deposition in Korean Patients With Cognitive Impairment. 韩国认知障碍患者的不确定潜能克隆性造血与脑β-淀粉样蛋白沉积之间的关系

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-05-28 DOI: 10.3343/alm.2024.0086

Jiwon Yun, Young Chul Youn, Hye Ryoun Kim

引用次数: 0

Effects of Various Concentrations of Pronase on Flow Cytometric Crossmatching Patients Treated With Rituximab and Donor HLA-Specific Antibodies. 不同浓度的 Pronase 对使用利妥昔单抗和供体 HLA 特异性抗体治疗的流式细胞术交叉配血患者的影响

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-12 DOI: 10.3343/alm.2024.0132

Tae-Shin Kim, Inseong Oh, Yu Jung Choi, Minjeong Nam, Hajeong Lee, Eun Young Song

{"title":"Effects of Various Concentrations of Pronase on Flow Cytometric Crossmatching Patients Treated With Rituximab and Donor HLA-Specific Antibodies.","authors":"Tae-Shin Kim, Inseong Oh, Yu Jung Choi, Minjeong Nam, Hajeong Lee, Eun Young Song","doi":"10.3343/alm.2024.0132","DOIUrl":"10.3343/alm.2024.0132","url":null,"abstract":"Background: Pronase pretreatment can reduce rituximab (RTX) interference by degrading CD20 in B-cell flow cytometry crossmatch (FCXM) testing. However, it may also reduce the assay sensitivity by degrading HLA molecules. We investigated the effects of various pronase concentrations on RTX interference and the analytical sensitivity of B-cell FCXM testing.Methods: Using 59 patient serum samples and 38 donor lymphocyte samples, we designed 97 recipient-donor pairs and divided them into three groups according to RTX use and the presence of weak-to-moderate donor HLA-specific antibody (DSA) reactions: RTX+/DSA-, RTX+/DSA+, and RTX-/DSA+. FCXM was performed after pretreating lymphocytes with six different pronase concentrations (0, 0.5, 1, 2, 3, and 4 mg/mL).Results: With B-FCXM testing, false-positive results due to RTX in the RTX+/DSA- group markedly decreased with increasing pronase concentrations. The median channel shift values in the RTX+/DSA+ and RTX-/DSA+ groups did not significantly decrease when the pronase concentration was increased from 1 mg/mL to 2 or 3 mg/mL. All eight RTX+/DSA+ cases that were positive at 1 mg/mL pronase but negative at 2 or 3 mg/mL had mean fluorescence intensity (MFI) DSA values of less than 3,000 except for DQ5 (MFI: 5,226). With T-cell FCXM, false-positive results were observed in 2.9% of 315 FCXM tests with pronase pretreatment.Conclusions: Higher concentrations (2 or 3 mg/mL) of pronase effectively eliminated RTX interference but still carried a risk for false negativity for weak DSA reactions in B-cell FCXM. Higher pronase concentrations can be used as an auxiliary method to detect moderate-to-strong DSA reactions in RTX-treated patients.","PeriodicalId":8421,"journal":{"name":"Annals of Laboratory Medicine","volume":" ","pages":"545-552"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a False-positive Multiplex Ligationdependent Probe Amplification Result in BRCA1 Using a Copy Number Variation Algorithm Under Development for a Commercial Next-Generation Sequencing-based Homologous Recombination Deficiency Assay. 利用正在开发的基于商用下一代测序的同源重组缺陷测定的拷贝数变异算法识别 BRCA1 的多重连接依赖性探针扩增假阳性结果。

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-05-31 DOI: 10.3343/alm.2024.0051

Paola Concolino, Elisa De Paolis, Martina Rinelli, Giulia Maneri, Francesca Brisighelli, Rita Trozzi, Simona Duranti, Luciano Giacò, Maria Piane, Alessia Preziosi, Arianna Panfili, Giovanni Scambia, Camilla Nero, Maria De Bonis, Angelo Minucci

引用次数: 0

An Unexpected Encounter During a Screening Colonoscopy in a Medical Tourist: A Diagnosis of Hymenolepis nana Infection. 一名医疗游客在接受结肠镜检查时的意外遭遇：诊断为 "海门螺杆菌（Hymenolepis nana）感染"。

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-22 DOI: 10.3343/alm.2024.0167

Bosung Park, Hye Won Park, Hyun Jung Park, Eun Jeong Won, Heungsup Sung, Mi-Na Kim

引用次数: 0

Effect of Blood Collection Tubes on Vitamin D Immunoassay Results. 采血管对维生素 D 免疫测定结果的影响

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-23 DOI: 10.3343/alm.2024.0234

Hyojin Chae, Sangyoon Lee, Ae-Ran Choi, Sung-Eun Cho, Eun-Jee Oh

引用次数: 0

Comment on "Ionized Magnesium Correlates With Total Blood Magnesium in Pediatric Patients Following Kidney Transplant". 关于 "肾移植后小儿患者电离镁与血镁总量的相关性 "的评论

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI: 10.3343/alm.2024.0153

Naveen Bangia, Dennis Begos, Bogdan Milojkovic

引用次数: 0

Why Terminology Standards Matter for Data-driven Artificial Intelligence in Healthcare. 为什么术语标准对医疗保健领域的数据驱动型人工智能至关重要？

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-03 DOI: 10.3343/alm.2024.0105

Hyeoun-Ae Park

引用次数: 0

Evaluation of Droplet Digital PCR for the Detection of BRAF V600E in Fine-Needle Aspiration Specimens of Thyroid Nodules. 评估用于检测甲状腺结节细针抽吸标本中 BRAF V600E 的液滴数字 PCR。

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-06-14 DOI: 10.3343/alm.2023.0405

Young Kyu Min, Jae Kyung Kim, Kyung Sun Park, Jong-Won Kim

{"title":"Evaluation of Droplet Digital PCR for the Detection of BRAF V600E in Fine-Needle Aspiration Specimens of Thyroid Nodules.","authors":"Young Kyu Min, Jae Kyung Kim, Kyung Sun Park, Jong-Won Kim","doi":"10.3343/alm.2023.0405","DOIUrl":"10.3343/alm.2023.0405","url":null,"abstract":"Background: Droplet digital (dd)PCR is a new-generation PCR technique with high precision and sensitivity; however, the positive and negative droplets are not always effectively separated because of the \"rain\" phenomenon. We aimed to develop a practical optimization and evaluation process for the ddPCR assay and to apply it to the detection of BRAF V600E in fine-needle aspiration (FNA) specimens of thyroid nodules, as an example.Methods: We optimized seven ddPCR parameters that can affect \"rain.\" Analytical and clinical performance were analyzed based on histological diagnosis after thyroidectomy using a consecutive prospective series of 242 FNA specimens.Results: The annealing time and temperature, number of PCR cycles, and primer and probe concentrations were found to be more important considerations for assay optimization than the denaturation time and ramp rate. The limit of blank and 95% limit of detection were 0% and 0.027%, respectively. The sensitivity of ddPCR for histological papillary thyroid carcinoma (PTC) was 82.4% (95% confidence interval [CI], 73.6%-89.2%). The pooled sensitivity of BRAF V600E in FNA specimens for histological PTC was 78.6% (95% CI, 75.9%-81.2%, I2=60.6%).Conclusions: We present a practical approach for optimizing ddPCR parameters that affect the separation of positive and negative droplets to reduce rain. Our approach to optimizing ddPCR parameters can be expanded to general ddPCR assays for specific mutations in clinical laboratories. The highly sensitive ddPCR can compensate for uncertainty in cytological diagnosis by detecting low levels of BRAF V600E.","PeriodicalId":8421,"journal":{"name":"Annals of Laboratory Medicine","volume":" ","pages":"553-561"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory Preparation for Digital Medicine in Healthcare 4.0: An Investigation Into the Awareness and Applications of Big Data and Artificial Intelligence. 医疗保健 4.0 中数字医学的实验室准备工作：对大数据和人工智能的认识与应用的调查。

IF 4 2区医学

Annals of Laboratory Medicine Pub Date : 2024-11-01 Epub Date: 2024-07-02 DOI: 10.3343/alm.2024.0111

Shinae Yu, Byung Ryul Jeon, Changseung Liu, Dokyun Kim, Hae-Il Park, Hyung Doo Park, Jeong Hwan Shin, Jun Hyung Lee, Qute Choi, Sollip Kim, Yeo Min Yun, Eun-Jung Cho

{"title":"Laboratory Preparation for Digital Medicine in Healthcare 4.0: An Investigation Into the Awareness and Applications of Big Data and Artificial Intelligence.","authors":"Shinae Yu, Byung Ryul Jeon, Changseung Liu, Dokyun Kim, Hae-Il Park, Hyung Doo Park, Jeong Hwan Shin, Jun Hyung Lee, Qute Choi, Sollip Kim, Yeo Min Yun, Eun-Jung Cho","doi":"10.3343/alm.2024.0111","DOIUrl":"10.3343/alm.2024.0111","url":null,"abstract":"Background: Healthcare 4.0. refers to the integration of advanced technologies, such as artificial intelligence (AI) and big data analysis, into the healthcare sector. Recognizing the impact of Healthcare 4.0 technologies in laboratory medicine (LM), we seek to assess the overall awareness and implementation of Healthcare 4.0 among members of the Korean Society for Laboratory Medicine (KSLM).Methods: A web-based survey was conducted using an anonymous questionnaire. The survey comprised 36 questions covering demographic information (seven questions), big data (10 questions), and AI (19 questions).Results: In total, 182 (17.9%) of 1,017 KSLM members participated in the survey. Thirty-two percent of respondents considered AI to be the most important technology in LM in the era of Healthcare 4.0, closely followed by 31% who favored big data. Approximately 80% of respondents were familiar with big data but had not conducted research using it, and 71% were willing to participate in future big data research conducted by the KSLM. Respondents viewed AI as the most valuable tool in molecular genetics within various divisions. More than half of the respondents were open to the notion of using AI as assistance rather than a complete replacement for their roles.Conclusions: This survey highlighted KSLM members' awareness of the potential applications and implications of big data and AI. We emphasize the complexity of AI integration in healthcare, citing technical and ethical challenges leading to diverse opinions on its impact on employment and training. This highlights the need for a holistic approach to adopting new technologies.","PeriodicalId":8421,"journal":{"name":"Annals of Laboratory Medicine","volume":" ","pages":"562-571"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141475829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0