Arthropod-Plant Interactions最新文献

{"title":"Attraction and oviposition responses of Phthorimaea absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) females to different tomato varieties","authors":"Ekrem Ögür","doi":"10.1007/s11829-025-10217-2","DOIUrl":"10.1007/s11829-025-10217-2","url":null,"abstract":"<div><p>The tomato leafminer, <i>Phthorimaea absoluta</i> (Meyrick, 1917) (Lepidoptera: Gelechiidae), is currently the major pest threatening cultivated tomato production worldwide, causing severe yield losses. This study focused on investigating the effects of the five tomato varieties (Taykan, Torry, Verty, Seyran, and Mamston) most preferred by producers in Antalya province in Türkiye on the attraction and oviposition responses of <i>P</i>. <i>absoluta</i> females. Y-tube olfactometer bioassays were conducted to determine the attraction of tomato varieties to mated <i>P</i>. <i>absoluta</i> females. Oviposition preference bioassays were carried out in cages to establish the effect of tomato varieties on the pest’s oviposition response. Volatile organic compounds of each variety were also identified by GC-MS. Mated <i>P. absoluta</i> females were most attracted to the Taykan variety and least attracted to the Mamston variety in the Y-tube olfactometer. In the oviposition choice and no-choice experiments conducted in cages, mated females laid the highest average number of eggs on Taykan (92.70 ± 4.02 and 84.40 ± 3.14, respectively) and the lowest on Mamston (41.30 ± 3.95 and 39.80 ± 3.08, respectively), with a statistically significant difference observed. In GC-MS, the most abundant compounds identified in all varieties were terpenes, followed by aldehydes. Principal component analysis on volatile organic compounds from tomato varieties revealed a 71.25% variance between varieties by two components. The presence of VOCs, namely caryophyllene, humulene, 2-carene, beta-phellandrene, and o-cymene could serve as attractant to mated <i>P</i>. <i>absoluta</i> females, whereas the presence of (E)-2-hexenal, hexadecane, nerone, and mequinol could have a repellent effect. Consequently, this study showed that tomato varieties significantly influenced female tomato leafminer attraction and oviposition responses. Accordingly, the findings presented herein may prove beneficial in the development of tomato varieties resistant or tolerant to <i>P</i>. <i>absoluta</i>.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145929775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological plant traits across the ontogeny of Phaseolus lunatus L. influence the insect community structure","authors":"Horacio Salomon Ballina−Gómez,&nbsp;Ana Paola Martínez−Falcón,&nbsp;Esaú Ruiz−Sánchez,&nbsp;Rubén Humberto Andueza−Noh,&nbsp;Alejandra González−Moreno,&nbsp;Jaime Martínez−Castillo,&nbsp;Roberto Rafael Ruiz−Santiago","doi":"10.1007/s11829-025-10216-3","DOIUrl":"10.1007/s11829-025-10216-3","url":null,"abstract":"<div><p>Studying interactions between plants and insects is crucial for understanding plant productivity in both natural and managed ecosystems. This background underscores the need for sustainable food production strategies addressing human health, environmental safety, and pesticide resistance. Moreover, predicting and evaluating changes in plant traits and ontogenetic plant traits is essential, as these factors influence the ecological outcomes of plant − insect interactions. This study aimed to analyze the relationship between morphological traits and insect communities—specifically herbivores, predators, and beneficial insects—across the ontogeny of lima bean (<i>Phaseolus lunatus</i> L.) in 25 landraces from the Yucatán Peninsula. Field data on phenotypic plant traits and insect family composition were collected for each landrace during two growth stages: vegetative (15 to 30 days after emergence) and reproductive (45 to 60 days after emergence). The variables most strongly related included color traits such as green hypocotyls and red or reddish-grey seeds, insect families, and insect guilds. Specifically, the color traits of cotyledons, hypocotyls, and seeds, along with specific leaf area, showed a notable association with the Chrysomelidae family. Our findings clarify the relationship between morphological plant traits and insect families, highlighting dynamics across two stages of lima bean ontogeny and enhancing our understanding of ecological relationships among diverse insect groups and their identities.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145930043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of resistance traits in bitter gourd genotypes against melon fruit fly (Bactrocera cucurbitae Coquillett): morphological and biochemical insights","authors":"Koushik Sen,&nbsp;Snigdha Samanta,&nbsp;Tanmoy Satpati,&nbsp;Partha Protim Dhar,&nbsp;Arunava Samanta","doi":"10.1007/s11829-025-10215-4","DOIUrl":"10.1007/s11829-025-10215-4","url":null,"abstract":"<div><p>The melon fruit fly, <i>Bactrocera cucurbitae</i> (Coquillett) is an important notorious pest of many cucurbitaceous vegetables including bitter gourd (<i>Momordica charantia</i>). It often causes substantial yield losses in the Gangetic Alluvial Zone of West Bengal, India. We expected that the genetic variability between bitter gourd genotypes is responsible for the varying degree of resistance to fruit fly infestation through distinct morpho-chemical traits. Field screenings (preliminary and final) of twenty bitter gourd genotypes revealed significant differences in fruit infestation and maggot density per fruit and identified five resistant (US-6214, Asmita, Meghnad-2, Vivek and Chandramukhi), nine moderately resistant (Noble Katahi, Palee, Contai Bolder, Mohar, PAN-1983, Chottu, Deb-520, Malaypuri and NS-454) and six susceptible genotypes (Karishma, Abhishek, Akash, Krishna Kantedar, Prachi and Pusa Do Mausami). Resistant genotypes exhibited antixenotic traits such as long tubercles, higher tubercle density, thicker pericarp and tougher fruits, and higher concentrations of protein, total sugar, ascorbic acid, tannin, flavonols, total phenols, peroxidase and polyphenol oxidase. These traits were negatively associated with fruit infestation and maggot density, while higher fruit moisture and chlorophyll contents in the susceptible genotypes were positively correlated. Multiple regression models revealed pericarp thickness, fruit toughness, tannin and flavonols accounted for maximum variation in fruit fly infestation among all morpho-chemical factors. Principal component analysis of morpho-chemical traits using varimax rotation with Kaiser normalization extracted four principal components (PCs), explaining 92.50% of the cumulative variance in fruit fly infestation. PC1, PC2, PC3 and PC4 contributed 62.45%, 15.83%, 9.28% and 4.94% of the total variation, respectively. These findings underscore the importance of leveraging genetic diversity to develop resistant bitter gourd cultivars. Such cultivars can reduce the cost of cultivation and enhance farmers’ income while serving as a critical component of integrated pest management strategies.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational reprogramming of Zea mays-fed larval midgut of Spodoptera litura indicates non-adaptive response","authors":"Archana Singh,&nbsp;Sumit Kumar,&nbsp;Kalpesh Nath Yajnik,&nbsp;Sujata Singh,&nbsp;Indrakant Kumar Singh","doi":"10.1007/s11829-025-10208-3","DOIUrl":"10.1007/s11829-025-10208-3","url":null,"abstract":"<div><p><i>Spodoptera litura</i>, commonly known as the tobacco cutworm or cotton leafworm, is a significant agricultural pest belonging to the family Noctuidae. Being polyphagous, it can feed on a wide variety of host plants and the larvae are particularly voracious feeders, causing substantial damage to crops. However, <i>Zea mays</i> (African tall variety) harbour a robust defense system and releases defensive proteins and allelochemicals that retard growth and development of <i>S. litura</i>. To identify and isolate the proteins responsive to maize allelochemicals, a comparative proteomic analysis of the mid-gut of maize-fed <i>S. litura</i> larvae was conducted. This analysis revealed the upregulation of 203 and downregulation of 376 differentially abundant proteins (DAPs). The DAPs were categorized into various functional groups, such as carbohydrate and lipid metabolism, defense, growth and development, transport, signaling, molecular processes, and digestion. The downregulation of proteins associated with digestion, detoxification, immunity and growth or development, suggests a non-adaptive mechanism against maize allelochemicals. This information can be crucial for understanding the impact of maize metabolites on insect gut and potentially exploiting it for pest management.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The tilted-head feeding strategy: ultramorphological adaptations of the mature larvae of Dolerus tritici (Hymenoptera: Tenthredinidae) to feeding on their host plants","authors":"Tian-Yu Yu,&nbsp;Ping Guan,&nbsp;Hao Zhang,&nbsp;Lu Jiang","doi":"10.1007/s11829-025-10214-5","DOIUrl":"10.1007/s11829-025-10214-5","url":null,"abstract":"<div><p>Larvae of the sawfly genus <i>Dolerus</i> are herbivorous, feeding on different species of graminoids. The larvae are notable for their asymmetrical labrum, an atypical trait among Hymenoptera, yet the associated feeding mechanism remains unclear. The wheat-preferring <i>Dolerus tritici</i> Chu provides key insights into this behavior. In this study, we investigated the feeding behavior and mouthpart ultrastructure of <i>D. tritici</i> larvae through field observations and scanning electron microscopy. For the first time, we identified the ultrastructural basis underlying their feeding mechanism. The key evidence came from distinct wear patterns, which indicated that three pairs of slanted cutting points were involved in leaf incision, while the maxillary and labial palps are responsible for positioning the leaf during this process. Ultramorphological evidence, including a notch in the left side of the labrum, mesally protruding incisors and molars on the left mandible, and mechanoreceptors on the penultimate maxillary palps, suggests a coordinated specialization of the larval feeding apparatus for feeding on plants with parallel-veined leaves. We propose that this tilted-head strategy represents an adaptation to feeding on straight, parallel-veined leaves.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bee pollination increases fruit weight and antioxidants in pumpkins","authors":"Isabelle Cristina Santos Magalhães,&nbsp;Gerlayne Teixeira de Souza,&nbsp;Marcelo da Rocha Souza,&nbsp;Liedson Tavares Carneiro,&nbsp;Girlaine Estéfane Cansanção de Almeida,&nbsp;Daniele Silva Ribeiro,&nbsp;Cibele Cardoso Castro","doi":"10.1007/s11829-025-10211-8","DOIUrl":"10.1007/s11829-025-10211-8","url":null,"abstract":"<div><p>Due to the global decline of pollinators, global food security is at risk. Crops essentially dependent on pollinators such as pumpkin (<i>Cucurbita moschata</i> Duchesne, Cucurbitaceae) are the most affected. Although the importance of pollinators for crop productivity is known, there are many gaps in knowledge regarding the influence of pollination on crop quality, especially on the chemical composition of fruits and seeds. Here, we evaluated the relationship between floral display, frequency of visits and crop production, using three pumpkin plantations in the semi-arid region of NE Brazil as a model. In all sites we recorded floral display, floral visitors, and compared fruit set, fruit quality and seed number between natural (NP) and cross (CP) pollination experiments. Bees were the main pollinators, and <i>Apis mellifera</i> and <i>Trigona spinipes</i> were the most frequent. Floral display was positively correlated with the total number of visits, which had a positive relation with fruit weight, specifically because of <i>T. spinipes</i> visits. Visitation by <i>A. mellifera</i> had a negative relationship with pericarp length. Fruit set was about 9% higher in NP than in CP, whose fruits had lower antioxidant activity than the former. Floral display was positively related to the frequency of visits of pumpkin produced in the semi-arid region of northeastern Brazil. Bees were the main pollinators, especially <i>T. spinipes</i> and <i>A. mellifera</i>, which positively influenced fruit weight. Thus, pollinators favored fruit set, weight, and quality, in addition to promoting antioxidant potential.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145730284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of water stress and common bean genotypes on biological aspects of Chrysodeixis includens and Trichogramma pretiosum","authors":"Eduardo Neves Costa,&nbsp;Marcos Gino Fernandes,&nbsp;Lucas Coutinho Reis,&nbsp;Andressa Caroline Foresti,&nbsp;Elias Soares Gomes,&nbsp;Silvana de Paula Quintão Scalon","doi":"10.1007/s11829-025-10213-6","DOIUrl":"10.1007/s11829-025-10213-6","url":null,"abstract":"<div><p>Water scarcity is expected to intensify, posing major challenges for crop production. Insect pests such as <i>Chrysodeixis includens</i> further reduce yields, especially in large monocultures. Water stress and plant resistance operate as bottom-up forces affecting herbivores and their natural enemies, whereas parasitoids impose top-down control. This study evaluated the effects of two common bean genotypes (a landrace variety and a commercial cultivar) on the growth and survival of <i>C. includens</i> under well-watered and water-stressed conditions. We also assessed whether the performance of the egg parasitoid <i>Trichogramma pretiosum</i> was influenced when developing in eggs from moths previously reared on the different genotypes and water regimes. Levels of hydrogen peroxide, malondialdehyde, and antioxidant enzymes were quantified. Water stress did not affect any biological parameter of the pest, although preliminary assays indicated reduced larval weight. The Manteigado genotype lowered larval survival by 27% and showed greater tolerance to <i>C. includens</i> defoliation compared with BRS Esplendor; however, neither genotype exhibited tolerance to water stress. None of the biological parameters of <i>T. pretiosum</i> were affected by bean genotype or water availability. Potential defense mechanisms were detected in Manteigado plants, including a 13–21% increase in superoxide dismutase activity, which detoxifies superoxide radicals, and a 21% reduction in total protein content, which may limit herbivore development. Manteigado therefore displays promising resistance traits for managing <i>C. includens</i> and may serve as a donor in breeding programs. Moreover, both genotypes appear compatible with <i>T. pretiosum</i> use even under water deficit, supporting sustainable pest management in bean crops.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"20 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145698541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical basis of host plant resistance in rapeseed and mustard against mustard aphid, Lipaphis erysimi (Kaltenbach)","authors":"Ajai Rangesan,&nbsp;Ngangom Uma Devi,&nbsp;Mougdha Dutta,&nbsp;Kennedy Ningthoujam","doi":"10.1007/s11829-025-10209-2","DOIUrl":"10.1007/s11829-025-10209-2","url":null,"abstract":"<div><p>Rapeseed-mustard (<i>Brassica</i> spp.) is a vital oilseed crop in India, frequently affected by the mustard aphid, <i>Lipaphis erysimi</i> (Kaltenbach), a major sap-sucking pest that causes substantial yield losses. Considering the drawbacks of chemical control, host plant resistance offers a sustainable alternative. The present study was conducted under protected conditions to assess the physical basis of resistance in ten rapeseed-mustard genotypes against <i>L. erysimi</i>. Significant genotypic variations were observed in aphid population, percentage of infested siliques, and aphid multiplication rate across crop stages. PM 25, PM 26, and DRMR 150-35 consistently recorded lower aphid infestations and were categorized as resistant, whereas IC 491023 and YST 151 were highly susceptible. Among the physical traits, surface wax content showed a strong negative correlation with aphid infestation and aphid multiplication, suggesting its critical role in resistance. PM 25 exhibited the highest wax content (7.30%), contributing to its superior resistance. The findings indicate that physical traits like surface wax act as a first line of defense by hindering aphid colonization and feeding. This study highlights the potential of integrating such traits into breeding programs, with PM 25 emerging as a promising genotype for developing aphid-resistant varieties.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"19 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145675157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of insect herbivory on phytoestrogen concentrations in three Trifolium species","authors":"Palash Mandal,&nbsp;Anna K. Wallingford,&nbsp;Marta R. M. Lima,&nbsp;Nicholas D. Warren,&nbsp;André F. Brito,&nbsp;Richard G. Smith","doi":"10.1007/s11829-025-10206-5","DOIUrl":"10.1007/s11829-025-10206-5","url":null,"abstract":"<div><p>Phytoestrogens are plant-produced secondary compounds believed to play a protective role against environmental stress. When consumed by animals, phytoestrogens can influence animal health and reproductive function; hence, there is interest in identifying factors that trigger their production in agricultural crops. We conducted two experiments to determine if leaf tissue herbivory by corn earworm (<i>Helicoverpa zea</i> Boddie) induces phytoestrogen accumulation in three clover species commonly utilized as livestock forage. In experiment I, insects were fed on red clover (<i>Trifolium pratense</i> L.) and kura clover (<i>T. ambiguum</i> L.) for 7 days. Herbivory increased total phytoestrogen levels by 112% in red clover, while levels in kura clover were extremely low and not influenced by herbivory. In experiment II, herbivory was applied to two red clover cultivars and one white clover cultivar (<i>T. repens</i> L.) for 9 days followed by a 9-day recovery period or for 18 days without recovery. After 9 days of herbivory and a 9-day recovery period, total phytoestrogen levels in red clover increased by 84%. Compared to the control, there were no differences in total phytoestrogen concentration when herbivory was applied to red clover for 18 days; however, additional sampling revealed concentrations were over 112% higher in undamaged compared to insect damaged tissues. Herbivory did not influence total phytoestrogen concentrations in white clover. These findings highlight the role insect herbivory can play in modifying the phytoestrogen status of red clover, and therefore a potentially novel mechanism by which insect herbivory may indirectly influence agricultural livestock health and reproductive function.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"19 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11829-025-10206-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145675193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Friends or betrayers? Beneficial effects of endophytic colonization of Amaranthus dubius on the reproductive traits of Spoladea recurvalis (Lepidoptera: Crambidae)","authors":"Druscilla O. Nyamamba,&nbsp;Josephine Karanja,&nbsp;Komivi S. Akutse","doi":"10.1007/s11829-025-10212-7","DOIUrl":"10.1007/s11829-025-10212-7","url":null,"abstract":"<div><p>Amaranth is a highly nutritious and medicinal crop with great potential for food security. However, its productivity is severely constrained by insect pests, particularly <i>Spoladea recurvalis</i>. Farmers’ reliance on chemical insecticides for its control poses high risks to human and environmental health. This study therefore explored the potential of fungal endophytes as a sustainable alternative approach in managing <i>S. recurvalis</i>. We screened 15 fungal isolates belonging to six different genera (<i>Trichoderma</i>, <i>Hypocrea</i>, <i>Beauveria</i>, <i>Fusarium</i>, <i>Bionectria</i> and <i>Metarhizium</i>) to assess their potential to endophytically colonize <i>Amaranthus dubius</i> through seed inoculation and determined the systemic induced resistance potential of the endophytes in controlling <i>S. recurvalis</i>. <i>Hypocrea lixii</i> F3ST1 and <i>Trichoderma asperellum</i> M2RT4 successfully colonized all the host seedling parts. <i>Hypocrea lixii</i> F3ST1 and <i>T. asperellum</i> M2RT4 were further investigated for their endophytic persistence, effect on plant growth, pathogenicity to <i>S. recurvalis</i>, and longevity of F1 progenies. Both endophytes remained persistent for the 4-week assessment period with varied colonization rates. The two endophytes significantly improved leaf number, leaf width, leaf length, plant height, and shoot dry weight. Additionally, <i>H. lixii</i> F3ST1 and <i>T. asperellum</i> M2RT4 significantly affected the life-history parameters of <i>S. recurvalis</i>, resulting in reductions in egg masses (5% and 49.8%, respectively; <i>p</i> &lt; 0.001), egg hatchability (7% and 48%, respectively; <i>p</i> &lt; 0.001), increased larval mortality (&gt; 35%), decreased number of pupae (4.9% and 58.4%, respectively; <i>p</i> &lt; 0.001), reduced adult emergence (4.9% and 57.9%, respectively; <i>p</i> &lt; 0.001), and lower survival of F1 progeny. Our findings suggest that <i>H. lixii</i> F3ST1 and <i>T. asperellum</i> M2RT4 may contribute to enhancing amaranth growth promotion and reducing <i>S. recurvalis</i> infestation under controlled conditions. However, further field evaluations are required to validate these effects under practical farming systems.</p></div>","PeriodicalId":8409,"journal":{"name":"Arthropod-Plant Interactions","volume":"19 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145675192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}