Applied Microbiology and Biotechnology最新文献

{"title":"Dynamic changes in oral microbiome composition during early childhood caries management","authors":"Yinbo Wang,&nbsp;Guiding Li,&nbsp;Yaopeng Liu,&nbsp;Juan Liu,&nbsp;Gang Li,&nbsp;Yanhong Li,&nbsp;Ni Zhou","doi":"10.1007/s00253-026-13928-5","DOIUrl":"10.1007/s00253-026-13928-5","url":null,"abstract":"<p>Early childhood caries (ECC) is a prevalent dental disease with significant public health burdens. Oral microbiota plays a key role in caries development. Although several studies have compared the oral microbiota before and after caries treatment, few have investigated the dynamic changes in oral microbial communities beyond 3 months. This 6-month prospective study monitored dynamic changes in the dental plaque microbiome, salivary pH, and oral hygiene status among children with ECC undergoing caries management. The outcomes were assessed at baseline (T0), immediately post-treatment (T1), and at 1-month, 3-month, and 6-month follow-ups (T2–T4). Children’s oral hygiene status improved significantly after ECC management (<i>p</i> &lt; 0.001). Their salivary pH levels increased at T1 but returned to the initial level during follow-up visits. Higher Chao1 scores were observed at T2, T3, and T4 when compared to T0 and T1 (<i>p</i> &lt; 0.001). The principal coordinate analysis revealed distinct clustering patterns between baseline and post-treatment visits. Significant differences were observed in the relative abundance of <i>Corynebacterium</i> (<i>p</i> &lt; 0.01), <i>Corynebacterium matruchotii</i> (<i>p</i> &lt; 0.01), and <i>Selenomonas noxia</i> (<i>p</i> &lt; 0.05) during the study period. The relative abundance of <i>Corynebacterium</i> and <i>Corynebacterium matruchotii</i> was significantly higher at T3 and T4 when compared to T1 (<i>p</i> &lt; 0.01). Additionally, <i>Saccharibacteria</i> was positively correlated with children’s dmfs scores and negatively correlated with children’s salivary pH values. The above findings possibly indicated that comprehensive ECC management might be associated with dynamic changes in the oral microbiome. Further well-designed randomized controlled trials are warranted to determine the impact of dental treatment on oral microbiota dynamics.</p><p>• <i>Dynamic changes observed in the oral microbiome following dental treatment</i></p><p>• <i>Abundance of Saccharibacteria associated with children’s caries status and salivary pH values</i></p><p>• <i>Children’s oral health–related behaviors improved after comprehensive dental management</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13928-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione peroxidase gene regulates heat stress response via trehalose synthesis in Pleurotus ostreatus","authors":"Guang Zhang,&nbsp;Jiajia Wang,&nbsp;Li Shang,&nbsp;Mengmeng Ding,&nbsp;Zhongwei Wu,&nbsp;Qian An,&nbsp;Yanjie Wang,&nbsp;Chaohui Zhang,&nbsp;Bin Chen","doi":"10.1007/s00253-026-13920-z","DOIUrl":"10.1007/s00253-026-13920-z","url":null,"abstract":"<p>Heat stress (HS) is a critical bottleneck restricting the commercial cultivation of <i>Pleurotus ostreatus</i>, severely compromising its yield and quality. Glutathione peroxidase (GPX) is a conserved antioxidant enzyme essential for stress adaptation, yet its regulatory mechanism in the fungal heat stress response (HSR) remains unclear. This study characterized a GPX homolog <i>PoGPX</i> in <i>P. ostreatus</i> and elucidated its functions in fungal growth, abiotic stress resistance, and HSR. <i>PoGPX</i> was downregulated during fruiting body development and upregulated under multiple abiotic stresses (especially HS). Silencing <i>PoGPX</i> promoted mycelial growth, biomass accumulation, and hyphal hyperbranching, accompanied by elevated intracellular reactive oxygen species (ROS) levels and compensatory changes in antioxidant enzyme activities. Notably, silencing <i>PoGPX</i> increased cytosolic trehalose content by 0.45–0.62-fold compared with the wild type (WT) strain, and this elevation was reversed by treatment with the ROS scavenger <i>N</i>-acetylcysteine, implying <i>PoGPX</i> modulates trehalose biosynthesis through ROS signaling. Functionally, silencing <i>PoGPX</i> alleviated HS-induced oxidative damage (reduced malondialdehyde content) and suppressed the upregulation of heat shock protein (HSP) genes expression (<i>HSP60</i>, <i>HSP90</i>, <i>HSP104</i>). Critically, inhibiting trehalose biosynthesis restored the HSR phenotypes in <i>PoGPX</i>-silenced strains, indicating trehalose as the key downstream mediator of <i>PoGPX</i>-regulated HSR. These findings uncover that <i>PoGPX</i> regulates HSR in <i>P. ostreatus</i> through trehalose biosynthesis induced by intracellular ROS levels. This study provides novel insights into fungal HSR mechanisms and identifies <i>PoGPX</i> as a promising target for genetic engineering to improve the thermotolerance of <i>P. ostreatus</i> in commercial cultivation.</p><p><i>• PoGPX acts as a key negative regulator on mycelial growth and hyphal branching in P. ostreatus.</i></p><p><i>• The PoGPX silencing alleviates the heat stress response of P. ostreatus.</i></p><p><i>• PoGPX regulates the heat stress response in P. ostreatus by modulating cytosolic trehalose biosynthesis.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13920-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analysis of RNA-binding proteins across some drug resistant and sensitive Staphylococcus aureus","authors":"Magda M. Awad,&nbsp;Yehia A. Osman,&nbsp;Mohamed Abdelmoteleb","doi":"10.1007/s00253-026-13906-x","DOIUrl":"10.1007/s00253-026-13906-x","url":null,"abstract":"<div><p>The adaptability and persistence of <i>Staphylococcus aureus</i> in diverse host environments may be influenced by its genetic repertoire, including RNA-binding proteins (RBPs). These proteins play critical roles in regulating post-transcriptional processes; however, their significance in bacteria, particularly in pathogenic strains, remains poorly understood. This gap necessitates a comprehensive exploration of RBPs in bacterial pathogens such as <i>S. aureus</i> better to understand their potential contributions to virulence and adaptability. In this study, a bioinformatics-based analysis of the genomes and proteomes of 51 pathogenic <i>S. aureus</i> strains was conducted, focusing on their RNA-binding proteome (RBPome). The pathogenicity of all analyzed strains was confirmed, and both known and putative virulence genes were identified. In addition, strains were categorized as drug-resistant or drug-sensitive based on genomic data, providing insights into their genetic variation. Phylogenetic analysis based on single-nucleotide polymorphism (SNP) data revealed distinct groupings of major clonal complexes, highlighting evolutionary relationships among the strains. Eight conserved RNA-binding proteins were identified, and their RNA-binding domains and molecular dynamics were predicted. Notable differences in molecular phylogeny, RNA-binding domains, and dynamics were observed in cold shock proteins A and B and S1 RNA-binding domain proteins between resistant and sensitive strains. These findings suggest that RNA-binding proteins may play important roles in the pathogenicity and adaptability of <i>S. aureus</i> and could be potential molecular targets for future therapeutic strategies.</p></div>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13906-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipopeptides efficiently disperse spore crystal aggregates in Bacillus thuringiensis israelensis","authors":"Maissa Chakroun,&nbsp;Sameh Sellami,&nbsp;Dalel Ben Farhat-Touzri,&nbsp;Nay El Khoury,&nbsp;Hadi Loutfi,&nbsp;Mireille Kallassy Awad,&nbsp;Souad Rouis","doi":"10.1007/s00253-026-13901-2","DOIUrl":"10.1007/s00253-026-13901-2","url":null,"abstract":"<div><p>The separation of <i>Bacillus thuringiensis</i> (<i>Bt</i>) spores and crystals is often compromised by dense aggregates, particularly in <i>Bt </i>subsp. <i>israelensis</i>. This study evaluates (sonication), chemical (SDS), and biological (lipopeptide) treatments to improve aggregate dissociation in BUPM98, using AR23 as a reference and Lip (<i>Bt </i>subsp. <i>kurstaki</i>) as a naturally dissociated control. Initial hexane-based purification highlighted the impact of aggregation, with significantly lower recovery rates for <i>israelensis</i> compared to <i>kurstaki</i>. Electron microscopic analysis revealed tightly integrated spore crystal complexes in <i>Bt israelensis</i> and independent bipyramidal crystals in Lip. While physical sonication and chemical SDS treatments improved dissociation, biological treatment with lipopeptides yielded the most significant results. This approach drastically increased viable spore counts by up to 8000-fold and enhanced crystal recovery to 60%, offering a superior and more efficient biosurfactant-based alternative to traditional methods. PCR analysis identified the kurstakin synthetase gene exclusively in the naturally dissociated <i>kurstaki</i> strain, suggesting a native role for these biosurfactants in preventing aggregation. To our knowledge, this is the first demonstration that lipopeptides can effectively dissociate <i>Bacillus thuringiensis</i> spore crystal aggregates, offering an efficient biosurfactant-based alternative to physical or chemical methods.</p></div>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13901-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of soybean meal enzymatic treatment on chicken caecal microbiota and intestinal epithelial cells","authors":"Lauriane Plouhinec,&nbsp;Marc Maresca,&nbsp;Julie Saget,&nbsp;Virginie Neugnot,&nbsp;Estelle Devillard,&nbsp;Jean-Guy Berrin,&nbsp;Mickael Lafond","doi":"10.1007/s00253-026-13925-8","DOIUrl":"10.1007/s00253-026-13925-8","url":null,"abstract":"<p>As demand for sustainable and welfare-conscious food production increases, reducing the environmental and health impacts of animal feed has become a key challenge. Soybean meal (SBM) is widely used in poultry diets for its high protein content, but its richness in non-starch polysaccharides (NSPs) can hamper its digestibility. To address this issue, a common strategy involves the utilization of enzymatic cocktails rich in carbohydrate-active enzymes (CAZymes). While these feed additives are specifically designed to degrade NSPs and enhance SBM protein digestibility, only scarce information is available on their impact on intestinal health. In this study, using intestinal epithelial cells lines, we show that pre-treatment of SBM by the enzymatic cocktail Rovabio™ Advance, supplemented or not with pectin-active fungal secretomes, did not alter epithelial integrity nor induce inflammatory responses. Additionally, in vitro chicken caecal fermentations revealed that SBM was associated with a higher relative abundance of <i>Enterobacteriaceae</i>, while wheat fermentation was associated with <i>Lactobacillaceae</i> and <i>Enterococcaceae</i> enrichment. Enzymatic supplementation with Rovabio™ Advance further enhanced wheat fermentation and bacterial community shifts, while its effect on SBM fermentation remained limited. In contrast, SBM pre-treatment with pectin-active fungal secretomes led to significantly increased short-chain fatty acid (SCFA) production and enriched SCFA-associated genera. These findings indicate that while industrial enzyme cocktails are well suited to cereal-based diets, targeted pectin-degrading enzymes could be seen as a promising strategy to enhance the prebiotic potential of SBM. This study highlights the importance of tailoring enzymatic solutions to feed carbohydrate composition, to improve gut microbiota function and promote intestinal health in poultry.</p><p>• <i>SBM does not induce intestinal cell inflammation or epithelium disruption in vitro.</i></p><p>• <i>Both SBM and wheat increase SCFA production by the chicken caecal microbiota.</i></p><p>• <i>Pre-treatment with fungal pectin-active secretomes enhance SBM’s prebiotic potential.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13925-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics reveal molecular changes during suspension adaptation of HEK293 cells","authors":"Benyao Zhang,&nbsp;Shishi Li,&nbsp;Jingjing Liu,&nbsp;Wenhao Su,&nbsp;Xiaohuan Zhang,&nbsp;Xiuxiu Ren,&nbsp;Tingting Zhao,&nbsp;Qiufang Huang,&nbsp;Zihao Ge,&nbsp;Jiangbo Wei","doi":"10.1007/s00253-026-13912-z","DOIUrl":"10.1007/s00253-026-13912-z","url":null,"abstract":"<p>Human embryonic kidney 293 (HEK293) cells have been successfully adapted from adherent to suspension culture and widely applied in both scientific research and the pharmaceutical industry. Although some studies investigated the variances between established adherent and suspension HEK293 cells of different strains, specific alterations in the cells during this consecutive process of suspension adaptation and possible factors driving this process have not been well described. Here, we adapted adherent HEK293 to suspension with desirable cell growth and high productivity for recombinant adenoviral vectors, and cells at several stages throughout the process were characterized. Slower cell growth, lower glucose uptake, increased lactate production, and weaker cell-surface adhesion were observed in suspension cells compared to their adherent counterparts. We further performed transcriptomics, proteomics, and metabolomics analysis to identify key cellular switches. A total of 2476 differentially expressed genes were found, including 1218 upregulated and 1258 downregulated genes in suspension cells. A similar and correlated pattern was observed in the proteomic study, and 702 differentially expressed metabolites were identified by untargeted metabolomics. In light of enrichment analysis, we summarized that HEK293 adherent cells survived and adapted to suspension culture via structural remodeling, metabolic shift and stress resistance. Our results provide a molecular enlightenment for suspension adaptation and potential directions for rational modification of HEK293 cell lines for future use.</p><p>• <i>Suspension adaptation reduced adhesion and reshaped the HEK293 cytoskeleton.</i></p><p>• <i>Multi-omics revealed metabolic rewiring and enhanced stress resistance.</i></p><p>• <i>An optimized suspension line outperformed an internal HEK293 suspension reference.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13912-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous production of a plant biostimulant in Streptomyces albidoflavus","authors":"Renata Sigrist,&nbsp;Te Chen,&nbsp;Marta Reventós Montané,&nbsp;Peter Gockel,&nbsp;Yijun Qiao,&nbsp;Mathias Jönsson,&nbsp;Zhijie Yang,&nbsp;Tilmann Weber,&nbsp;Ling Ding,&nbsp;Emre Özdemir,&nbsp;Lei Yang","doi":"10.1007/s00253-026-13915-w","DOIUrl":"10.1007/s00253-026-13915-w","url":null,"abstract":"<p>Climate change–associated abiotic stresses threaten agricultural productivity, creating a need for sustainable strategies that improve plant resilience. Pteridic acids F and H (PTA-F and PTA-H), originally isolated from <i>Streptomyces iranensis</i> HM 35, are plant growth–promoting polyketides with reported activity under drought and salinity stress. However, reported production was extremely low (~ 0.08 and 0.02 mg/L), limiting further development and application. Here, we established a heterologous production platform for PTA biosynthesis by cloning the 68-kb type I polyketide synthase biosynthetic gene cluster using Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination (CAPTURE), followed by CRISPR-Cas9-mediated genomic integration and promoter engineering in <i>Streptomyces</i> hosts. Initial heterologous expression resulted in detectable elaiophylin production but not PTA, whereas BGC engineering with the strong constitutive <i>kasO</i>p* promoter enabled PTA production (although below the limit of quantification). Genome-scale metabolic model–guided media optimization further improved production and fed-batch fermentation yielded 1.7 mg/L PTA in J1074-PTA-<i>kasO</i>p* and 2.8 mg/L PTA in NBC1270-PTA-<i>kasO</i>p*. These titers represent a more tha n 20-fold increase compared with the native producer under comparable conditions. This work provides the first functional heterologous platform for PTA biosynthesis and demonstrates how synthetic biology and genome-scale metabolic modeling can be combined to improve production of complex plant-beneficial polyketides.</p><p>• <i>Direct BGC cloning and engineering enabled production of PTA in heterologous host.</i></p><p>• <i>Genome-scale metabolic models (GEMs) guided media optimization for PTA production.</i></p><p>• <i>Fed-batch fermentation achieved</i> &gt; <i>20-fold PTA titer improvement over native strain.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13915-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona","authors":"Xinying Li,&nbsp;Jingnan Wang,&nbsp;Chang Su,&nbsp;Chao Guo,&nbsp;Zhouqin Xu,&nbsp;Kehui Wang,&nbsp;Jian Pang,&nbsp;Bo Lv,&nbsp;Chao Wang,&nbsp;Chun Li","doi":"10.1007/s00253-026-13913-y","DOIUrl":"10.1007/s00253-026-13913-y","url":null,"abstract":"","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13913-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148270000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen-based soft-sensing of glucose demand in Chinese hamster ovary fed-batch cultures across scales","authors":"Valentin Jossen,&nbsp;Adrian Joseph,&nbsp;Gonzalo Mesado,&nbsp;Siewhoon Lim,&nbsp;Gilles Broccard","doi":"10.1007/s00253-026-13882-2","DOIUrl":"10.1007/s00253-026-13882-2","url":null,"abstract":"<p>Glucose is a critical nutrient in mammalian cell culture processes, influencing cellular metabolism, productivity, and product quality. In industrial Chinese hamster ovary (CHO) fed-batch processes, glucose concentrations are commonly controlled using manual offline measurements and operator-based feed adjustments. Although online Process Analytical Technology (PAT) solutions and oxygen uptake rate (OUR)-based feeding approaches have previously been investigated, their implementation in existing Good Manufacturing Practice (GMP) manufacturing environments remains challenging due to instrumentation, validation, and process integration requirements. In this study, the relationship between cumulative oxygen consumption and glucose utilization was investigated across 58 CHOK1SV GS-KO® fed-batch cultures performed in stirred glass and single-use bioreactors ranging from 10 L to 2 kL scale. Building on previously reported oxygen-based glucose estimation concepts, a lactate-adjusted framework was developed to account for both lactate production and re-consumption during the fed-batch process. Oxygen transfer rates were calculated from standard online process parameters and integrated over time to determine cumulative oxygen consumption, which was subsequently correlated with cumulative glucose utilization. The results demonstrated robust linear correlations between cumulative oxygen consumption and lactate-adjusted cumulative glucose utilization across different projects, cell lines, and process scales (<i>R</i>2 ≥ 0.984). Incorporation of lactate metabolism improved the robustness of the oxygen-glucose relationship, particularly during metabolic transitions associated with lactate re-consumption. The predictive capability of the framework was further evaluated in three 200 L fed-batch processes by prospectively estimating glucose consumption over sequential 24 h intervals using cumulative oxygen consumption and routine offline metabolite measurements. Overall, the study demonstrates that cumulative oxygen consumption provides a robust and scalable basis for oxygen-based soft-sensing of glucose demand in industrial CHO fed-batch cultures. The presented framework enables predictive glucose demand estimation using standard bioreactor process data without requiring additional glucose-specific PAT hardware and may support future implementation of automated feeding concepts in mammalian cell culture processes.</p><p>• <i>Cumulative oxygen consumption correlates linearly with glucose utilization.</i></p><p>• <i>Lactate-adjusted O₂–glucose correlations are consistent across bioreactor scales.</i></p><p>• <i>Glucose control can be automated using existing bioreactor process data.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13882-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production","authors":"Filip Opaterny,&nbsp;Rachel Javorova,&nbsp;Michal Soral,&nbsp;Lubomira Feckova,&nbsp;Dominika Csolleiova,&nbsp;Renata Novakova,&nbsp;Kamila Cholvadtova,&nbsp;Iveta Uhliarikova,&nbsp;Maria Kopacova,&nbsp;Vladimir Patoprsty,&nbsp;Bronislava Rezuchova,&nbsp;Beatrica Sevcikova,&nbsp;Jan Kormanec","doi":"10.1007/s00253-026-13908-9","DOIUrl":"10.1007/s00253-026-13908-9","url":null,"abstract":"<p>The genome of <i>Streptomyces lavendulae</i> subsp. <i>lavendulae</i> CCM 3239 contains 36 biosynthetic gene clusters (BGCs) that are cryptic or silent, except for the previously characterized aromatic polyketide auricin. In this work, we present a simple strategy to activate BGCs in this strain by replacing the native promoter with the strong <i>kasOp*</i> promoter via homologous recombination. This approach was validated by strong indigoidine production following insertion of <i>kasOp*</i> upstream of the silent <i>bpsA</i> gene. Using this strategy, we activated a silent BGC encoding a type I polyketide synthase (PKS). Interestingly, insertion of <i>kasOp*</i> at different positions within this BGC—upstream of the <i>SLAV_02840</i> and <i>SLAV_02776</i> genes—led to the production of distinct compounds, both exhibiting polyene spectral features and antifungal activity. Both compounds were isolated, and structural analysis revealed that they were similar to strevertenes; consequently, they were named strevertene A2 and X. A database search indicated that their structures corresponded to the known compounds RKGS-A2215A and AB023a. In silico analysis of the biosynthetic enzymes encoded by this strevertene (<i>stv</i>) BGC enabled us to propose a biosynthetic model consistent with the observed induction in the mutant strains <i>S. lavendulae kasOp::2776</i> and <i>S. lavendulae kasOp::2840</i>. The analysis also suggested that strevertene X is an intermediate in the formation of the final polyene, strevertene A2. Notably, strevertene X exhibited approximately twofold higher antifungal activity than strevertene A2.</p><p>• <i>Strategy for activating BGCs by introducing a strong kasOp* promoter.</i></p><p>• <i>Efficient activation of the silent bpsA gene for indigoidine.</i></p><p>• <i>Activation of silent BGC for type I PKS for polyenes strevertene A2 and X.</i></p>","PeriodicalId":8342,"journal":{"name":"Applied Microbiology and Biotechnology","volume":"110 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00253-026-13908-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148257109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}