Archives of histology and cytology最新文献_第8页

Type IV collagen alpha chains of the basement membrane in the rat bronchioalveolar transitional segment. 大鼠细支气管肺泡过渡段基底膜的IV型胶原α链。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.185

Noriko Hinenoya, Ichiro Naito, Ryusuke Momota, Yoshikazu Sado, Kanae Kumagishi, Yoshifumi Ninomiya, Aiji Ohtsuka

{"title":"Type IV collagen alpha chains of the basement membrane in the rat bronchioalveolar transitional segment.","authors":"Noriko Hinenoya, Ichiro Naito, Ryusuke Momota, Yoshikazu Sado, Kanae Kumagishi, Yoshifumi Ninomiya, Aiji Ohtsuka","doi":"10.1679/aohc.71.185","DOIUrl":"https://doi.org/10.1679/aohc.71.185","url":null,"abstract":"In the present study, we have analyzed the alpha(IV) chain distribution in the subepithelial basement membrane (BM) of the rat pulmonary airway from the bronchi to alveoli. We have furthermore analyzed the alpha(IV) chain distribution in the subepithelial BM of the bronchioalveolar duct junction (BADJ) using alpha(IV) chain specific monoclonal antibodies. Our results show that the BM of the bronchial and bronchiolar epithelium contains [alpha1(IV)]2alpha2(IV) and [alpha5(IV)]2alpha6(IV) molecules and confirmed that the alveolar BM consists of [alpha1(IV)]2alpha2(IV) and alpha3(IV) alpha4(IV)alpha5(IV) molecules. There are also small regions in BADJ consisting of only [alpha1(IV)]2alpha2(IV) molecules without alpha3(IV)alpha4(IV)alpha5(IV) and [alpha5(IV)]2alpha6(IV) molecules. Moreover, the bronchioalveolar stem cells (BASCs)-primordial cells for bronchiolar Clara cells and alveolar type II (AT2) cells - lie adjacent to such small regions. These findings suggest that [alpha1(IV)]2 alpha2(IV) may be important for the BASCs to self-renew or to self-maintain themselves and that microenvironments produced by alpha(IV) chains may be important for cell differentiation.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Transvascular accumulation of Sialyl Lewis X conjugated liposome in inflamed joints of collagen antibody-induced arthritic (CAIA) mice. Sialyl Lewis X偶联脂质体在胶原抗体诱导关节炎(CAIA)小鼠炎症关节中的经血管积累。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.195

Jun Minaguchi, Toshitaka Oohashi, Kiichi Inagawa, Aiji Ohtsuka, Yoshifumi Ninomiya

{"title":"Transvascular accumulation of Sialyl Lewis X conjugated liposome in inflamed joints of collagen antibody-induced arthritic (CAIA) mice.","authors":"Jun Minaguchi, Toshitaka Oohashi, Kiichi Inagawa, Aiji Ohtsuka, Yoshifumi Ninomiya","doi":"10.1679/aohc.71.195","DOIUrl":"https://doi.org/10.1679/aohc.71.195","url":null,"abstract":"The aim of the current study was to investigate the specific accumulation of the Sialyl Lewis X (SLX) liposome in inflammation in the collagen-antibody induced arthritic (CAIA) model mice. The SLX-liposome encapsulating fluorescent substance (Cy5.5 or Cy3) was prepared for this study. The SLX-liposome was administered intravenously via the mouse caudal vein. After 1 to 24 h, the accumulation of SLX-liposome was observed using in vivo fluorescent imaging equipment (eXplore Optix), or the knee joints were removed for histological analysis. The in vivo fluorescent imaging showed that the signal was confined to the inflammatory site in the CAIA mice in an inflammatory dependent manner. The signal intensity was stronger at 24 h than at 1 h after injection. In the histological sections, the fluorescent signals were detected in the periarticular soft-tissue, especially in the hyperplastic synovium, including a pannus invasion with inflammatory cells in the CAIA. Intense signals were observed in vessel-like structures 1 h after injection; these were co-labeled with the vascular endothelial cell marker (CD31) and E-selectin, a ligand of the SLX-liposome expressed on activated endothelial cells. The diffused signals from the vessels increased time-dependently at 6 to 24 h after injection. This is the first report to examine the exact localization of the SLXliposome by encapsulated fluorescence in hyperplastic synovial tissue of CAIA mice. These results suggest the feasibility and potential use of SLX-liposome as a vehicle for the active targeting of drug delivery to inflammatory tissue.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"195-203"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Surface morphology of the central macrophages of erythroblastic islets in the spleen of aged and pregnant mice: an immunohistochemical light microscopic study. 老年和妊娠小鼠脾红细胞胰岛中央巨噬细胞表面形态:免疫组织化学光镜研究。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.155

Yuji Sonoda, Kazunobu Sasaki

{"title":"Surface morphology of the central macrophages of erythroblastic islets in the spleen of aged and pregnant mice: an immunohistochemical light microscopic study.","authors":"Yuji Sonoda, Kazunobu Sasaki","doi":"10.1679/aohc.71.155","DOIUrl":"https://doi.org/10.1679/aohc.71.155","url":null,"abstract":"This study used 100-mum thick paraffin sections stained by the ER-HR3 antibody to examine the three-dimensional surface morphology of the central macrophages of erythroblastic islets in the splenic red pulp of aged and pregnant mice. The ER-HR3-positive cells were the macrophages located at the center of the erythroblastic islets, and the number per unit of splenic area was almost constant until 30 days of age, thereafter showing a marked decrease. In pregnant females, the ER-HR3-positive macrophage number significantly increased and became approximately eight times higher than the control value. In aged virgin females, the islet macrophages were generally ovoid in cell profile, and shallow cup-shaped dents were formed on their cell surface. However, in pregnant females, the macrophages became larger in size, and cell socket structures, formed by long finger-like cytoplasmic processes, became prominent on their cell surface. The 3-D images, obtained from 100-mum thick paraffin sections, provided the clear morphological evidence of the activity of the islet macrophages in spleen erythropoiesis.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium. 连接蛋白26和43在大鼠牙龈上皮中的差异表达和定位。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.147

Takashi Muramatsu, Tomoko Uekusa, Takayasu Masaoka, Masato Saitoh, Sadamitsu Hashimoto, Yoshihiro Abiko, Han-Sung Jung, Masaki Shimono

{"title":"Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium.","authors":"Takashi Muramatsu, Tomoko Uekusa, Takayasu Masaoka, Masato Saitoh, Sadamitsu Hashimoto, Yoshihiro Abiko, Han-Sung Jung, Masaki Shimono","doi":"10.1679/aohc.71.147","DOIUrl":"https://doi.org/10.1679/aohc.71.147","url":null,"abstract":"We investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immuno-electron microscopy, gap junctions appeared curved, hemi-circular, or annular within the cytoplasm, and gold particles indicating the presence of CX43 were localized at the outer edges of these cytoplasmic formations. These results suggest that CX43 is associated with the regulation of cell proliferation and that increased CX26 expression is associated with differentiation of keratinocytes. Thus, degradation of CX43 is considered to play an essential role in differentiation of the rat gingival epithelium.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"147-54"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The inhibition of apoptosis by glycyrrhizin in hepatic injury induced by injection of lipopolysaccharide / D-galactosamine in mice. 甘草酸对脂多糖/ d -半乳糖胺致小鼠肝损伤细胞凋亡的抑制作用。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.163

Tadayuki Ikeda, Kazuki Abe, Noriyuki Kuroda, Yujiro Kida, Hideo Inoue, Kenjiro Wake, Mitsuhiko Morito, Tetsuji Sato

{"title":"The inhibition of apoptosis by glycyrrhizin in hepatic injury induced by injection of lipopolysaccharide / D-galactosamine in mice.","authors":"Tadayuki Ikeda, Kazuki Abe, Noriyuki Kuroda, Yujiro Kida, Hideo Inoue, Kenjiro Wake, Mitsuhiko Morito, Tetsuji Sato","doi":"10.1679/aohc.71.163","DOIUrl":"https://doi.org/10.1679/aohc.71.163","url":null,"abstract":"The inhibition of apoptosis by glycyrrhizin (GL) in hepatic injury induced by injection of lipopolysaccharide (LPS)/D-galactosamine (D-GalN) was examined in the present study. Morphological and biochemical analyses of LPS/D-GalN-induced mouse liver injury revealed that apoptosis occurred exclusively in injured hepatocytes of the centrilobular area. The degree of hepatic injury was associated with a substantial number of hepatocytes undergoing apoptosis. Transaminase levels were significantly increased at 6 to 8 h after the injection of LPS/D-GalN compared with controls. GL inhibited the elevation of serum transaminase levels when it was given to mice at 30 min before the administration of LPS/D-GalN. Morphological analyses using the TUNEL-method showed GL significantly reduced the number of TUNEL-labeled cells in acute hepatitis induced with LPS/D-GalN-treatment. Cells from the pericentral hepatic injury region were dissected out using a microdissection-method, and the DNA-ladder was clearly documented. Furthermore, results obtained through the TUNEL-method were confirmed with an oligonucleosome-bound DNA ELISA. From the current results, it seems reasonable to conclude that the protective role of GL in LPS/D-GalN-induced liver injury is performed through the inhibition of hepatic apoptosis.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"163-78"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Immunohistochemical localization of protease-activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity. 蛋白酶激活受体在大鼠脑和睾丸小动脉中的免疫组织化学定位:它们对小动脉大小和器官特异性的依赖。

Archives of histology and cytology Pub Date : 2008-11-01 DOI: 10.1679/aohc.71.179

Toshinari Misaki, Yoh-Ichi Satoh, Tomoyuki Saino, Takashi Kuroda, Kazuki Masu, D A Russa, Akira Ogawa

{"title":"Immunohistochemical localization of protease-activated receptors in cerebral and testicular arterioles of rats: their dependence on arteriole size and organ-specificity.","authors":"Toshinari Misaki, Yoh-Ichi Satoh, Tomoyuki Saino, Takashi Kuroda, Kazuki Masu, D A Russa, Akira Ogawa","doi":"10.1679/aohc.71.179","DOIUrl":"https://doi.org/10.1679/aohc.71.179","url":null,"abstract":"Protease-activated receptors (PARs) expressed in the endothelia and smooth muscles of vessels may play important roles in blood vessel function. Using intracellular calcium ion concentration ([Ca2+]i) imaging, we recently observed that small - but not large - arterioles of the brain responded to proteases, while testicular arterioles showed no response. The purpose of the present study was to examine the heterogeneity of the localization of PARs in arterioles using immunohistochemistry. Consistent with the [Ca2+]i imaging results, neither the thrombin receptor nor PAR2 were evident in large arterioles of the brain. However, the small arterioles of the brain, vascular smooth muscles, and endothelia showed a distinct immunoreactivity against the thrombin receptor and PAR2. The immunoreactivity of PARs in testicular arterioles was faint. In conclusion, size-dependent and/or organ-specific responses of arterioles to proteases are due to the heterogeneous localization of PARs.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 3","pages":"179-84"},"PeriodicalIF":0.0,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.71.179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27965963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Structure and function of rat lymph nodes. 大鼠淋巴结的结构和功能。

Archives of histology and cytology Pub Date : 2008-09-01 DOI: 10.1679/AOHC.71.69

O. Ohtani, Y. Ohtani

{"title":"Structure and function of rat lymph nodes.","authors":"O. Ohtani, Y. Ohtani","doi":"10.1679/AOHC.71.69","DOIUrl":"https://doi.org/10.1679/AOHC.71.69","url":null,"abstract":"The lymph node comprises a critical crossroad for encounters between antigen presenting cells, antigens from lymph, and lymphocytes recruited into lymph nodes from the blood. The node consists of spaces lined with lymphatic endothelial cells and parenchyma. The former spaces can be divided into the subcapsular sinuses, lymphatic labyrinths in the deep cortex, intermediate sinuses, and medullary sinuses. The sponge-like framework of the node parenchyma is composed of collagen fibers invested with reticular cells. The parenchyma can be divided into the cortex, deep cortex, and medullary cord. Lymphocytes migrate from the node parenchyma into the lymphatic labyrinths in the deep cortex. Close to the labyrinths are high endothelial venules (HEVs), through which circulating lymphocytes enter the node parenchyma. HEVs strongly express Aquaporin-1, suggesting that HEVs are involved in the net absorption of water, but not protein, from lymph coming through afferent lymphatics. Many LYVE-1 positive sinus reticular cells (i.e., lymphatic endothelial cells) with attached macrophages form a network within the lumen of the medullary sinuses. Fluids and migrating cells arriving at the node preferentially flow through the subcapsular sinuses, intermediate sinuses, and medullary sinuses in this order. Fluids and migrating cells may also enter the cortex through gaps in the floor of the subcapsular sinuses.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 2 1","pages":"69-76"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.71.69","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 77

An electron microscopic study on nerve endings on adrenomedullary adrenaline cells in golden hamsters: position, size and changes due to pinealectomy. 电镜观察金仓鼠肾上腺髓内肾上腺素细胞神经末梢的位置、大小及松果体切除后的变化。

Archives of histology and cytology Pub Date : 2008-09-01 DOI: 10.1679/AOHC.71.115

T. Yamauchi, T. Kachi

{"title":"An electron microscopic study on nerve endings on adrenomedullary adrenaline cells in golden hamsters: position, size and changes due to pinealectomy.","authors":"T. Yamauchi, T. Kachi","doi":"10.1679/AOHC.71.115","DOIUrl":"https://doi.org/10.1679/AOHC.71.115","url":null,"abstract":"Effects of sham-pinealectomy and pinealectomy on preganglionic nerve endings on adrenomedullary adrenaline cells were investigated electron microscopically. Adult male golden hamsters from the normal, sham-pinealectomy and pinealectomy groups maintained under 24 h light-dark cycle and constant temperature were used at 28 days after surgery. From conventional electron microscopic specimens, montage photographs made of the adrenaline cell region at a magnification of x 11,000 were used for qualitative and quantitative electron microscopic analyses in 14 animals in each experimental group. The preganglionic nerve endings were localized mainly in the following three sites: the basal lamina part, the follicular lumen-junctional intercellular part, and the adrenaline cell-invaginated part. In the latter two parts, nerve endings and fibers had no envelope frequently, and in the former two parts, nerve endings sometimes showed the invagination complex. The frequency of nerve endings was highest in the follicular lumen-intercellular part, next highest in the basal lamina part and lowest in the A cell-invaginated part. The frequency of nerve endings in the basal lamina part was lower in the pinealectomy group than in the sham-pinealectomy group (P < 0.021), and those in the other two parts showed opposite changes, more evidently in the A cell-invaginated part. Nerve ending profiles in the adrenaline cell-invaginated part--which displayed a more rounded shape--increased in size in the pinealectomy group (longer diameter: P < 0.04; shorter diameter: P < 0.05). In conclusion, preganglionic nerve endings in the adrenal medulla of the golden hamster show differential morphological changes following PX depending on the intracellular part of A cells.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 2 1","pages":"115-22"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.71.115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of expression patterns of cathepsin K and MMP-9 in odontoclasts and osteoclasts in physiological root resorption in the rat molar. 组织蛋白酶K和MMP-9在大鼠磨牙生理性牙根吸收破骨细胞和破牙细胞中的表达规律比较。

Archives of histology and cytology Pub Date : 2008-09-01 DOI: 10.1679/AOHC.71.89

M. Tsuchiya, Y. Akiba, I. Takahashi, Y. Sasano, J. Kashiwazaki, Shinobu Tsuchiya, Makoto Watanabe

{"title":"Comparison of expression patterns of cathepsin K and MMP-9 in odontoclasts and osteoclasts in physiological root resorption in the rat molar.","authors":"M. Tsuchiya, Y. Akiba, I. Takahashi, Y. Sasano, J. Kashiwazaki, Shinobu Tsuchiya, Makoto Watanabe","doi":"10.1679/AOHC.71.89","DOIUrl":"https://doi.org/10.1679/AOHC.71.89","url":null,"abstract":"Root resorption lacunae are principally formed by odontoclasts. While these cells develop from the same origin as osteoclasts, odontoclasts normally have fewer nuclei and a less clear zone compared with osteoclasts. We therefore, hypothesized that odontoclasts possess less differentiation in matrix resorption characteristics than osteoclasts. To test our hypothesis, we compared the TRAP-positive area and the expression patterns of two important proteolytic enzymes, cathepsin K and matrix metalloproteinase-9 (MMP-9), between odontoclasts and osteoclasts. We focused on physiological root resorption in the rat molar, which is a useful experimental model for estimating odontoclasts and osteoclasts. Observations showed the number of nuclei and the TRAP-positive area of odontoclasts to be significantly less compared with osteoclasts. Using in situ hybridization and double labeling fluorescence in situ hybridization showed the majority of odontoclasts to express both cathepsin K and MMP-9, especially 4 and 5 weeks of age, when physiological root resorption occurs actively. Moreover, putative precursor cells of odontoclasts, which typically appeared in the middle of the periodontal ligament at 3 weeks of age, expressed both enzymes. In contrast, the majority of matured osteoclasts expressed only cathepsin K but not MMP-9. We suggest that odontoclasts are comparable to osteoclasts with less differentiation with regard to the expression of proteolytic enzymes.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 2 1","pages":"89-100"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.71.89","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Chelatable Fe (II) is generated in the rat kidneys exposed to ischemia and reperfusion, and a divalent metal chelator, 2, 2'-dipyridyl, attenuates the acute ischemia/reperfusion-injury of the kidneys: a histochemical study by the perfusion-Perls and -Turnbull methods. 可螯合铁(II)在暴露于缺血和再灌注的大鼠肾脏中产生，二价金属螯合剂2,2 '-双吡啶可减轻肾脏的急性缺血/再灌注损伤:灌注- perls和-Turnbull方法的组织化学研究。

Archives of histology and cytology Pub Date : 2008-09-01 DOI: 10.1679/AOHC.71.101

Hiroyasu Iwatsuki, R. Meguro, Yoshiya Asano, S. Odagiri, Chengtai Li, K. Shoumura

{"title":"Chelatable Fe (II) is generated in the rat kidneys exposed to ischemia and reperfusion, and a divalent metal chelator, 2, 2'-dipyridyl, attenuates the acute ischemia/reperfusion-injury of the kidneys: a histochemical study by the perfusion-Perls and -Turnbull methods.","authors":"Hiroyasu Iwatsuki, R. Meguro, Yoshiya Asano, S. Odagiri, Chengtai Li, K. Shoumura","doi":"10.1679/AOHC.71.101","DOIUrl":"https://doi.org/10.1679/AOHC.71.101","url":null,"abstract":"The perfusion-Perls and -Turnbull methods supplemented by diaminobenzidine intensification demonstrated the generation and localization of chelatable Fe (II) which can catalyze the generation of cytotoxic hydroxyl radicals (OH.) during the Fenton reaction in rat kidneys exposed to 40 min ischemia or 40 min-ischemia followed by 60 min-reperfusion. The kidneys exposed to 40 min-ischemia showed Fe (II)-deposits largely localized in the deeper half of the cortex, where the deposits densely filled the tubular cell nuclei, with a small amount of them in the cytoplasm of the proximal convoluted tubules (PCT). Intraluminally protruded or exfoliated tubular cell nuclei were also filled with the deposits. The kidneys subjected to 40 min-ischemia/ 60 min-reperfusion showed a more extensive distribution of Fe (II)-deposits, including most depths of the cortex. Furthermore, there were numerous exfoliated, Fe (II)-positive nuclei surrounded by a small amount of cytoplasm in the lumen of the PCT. These cells appeared to undergo apoptotic cell death since the lumen of strongly dilated, down-stream, proximal straight tubules were obstructed with numerous apoptotic cells in the kidneys exposed to 40 min-ischemia and 24 h-reperfusion. Pretreatment with a divalent metal chelator, 2, 2'-dipyridyl, effectively inhibited Fe (II)-staining, decreased the number of exfoliated cells in the kidneys with 40 min-ischemia/ 60 m-reperfusion, and decreased the number of apoptotic cells in the kidneys with 40 min-ischemia/24 h-reperfusion. The generation of highly reactive OH. during the Fe2+-catalyzed Fenton reaction was suggested to play a crucial role in ischemia/reperfusion-induced kidney injury.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"71 2 1","pages":"101-14"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.71.101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67483802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8