Archives of histology and cytology最新文献

{"title":"Coding channels for taste perception: information transmission from taste cells to gustatory nerve fibers.","authors":"Ryusuke Yoshida,&nbsp;Keiko Yasumatsu,&nbsp;Noriatsu Shigemura,&nbsp;Yuzo Ninomiya","doi":"10.1679/aohc.69.233","DOIUrl":"https://doi.org/10.1679/aohc.69.233","url":null,"abstract":"<p><p>Taste signals are first detected by the taste receptor cells, which are located in taste buds existing in the tongue, soft palate, larynx and epiglottis. Taste receptor cells contact with the chemical compounds in oral cavity through the apical processes which protrude into the taste pore. Interaction between chemical compounds and the taste receptor produces activation of taste receptor cells directly or indirectly. Then the signals are transmitted to gustatory nerve fibers and higher order neurons. A recent study demonstrated many similarities between response properties of taste receptor cells with action potentials and those of the gustatory nerve fibers innervating them, suggesting information derived from receptor cells generating action potentials may form a major component of taste information that is transmitted to gustatory nerve fibers. These findings may also indicate that there is no major modification of taste information sampled by taste receptor cells in synaptic transmission from taste cells to nerve fibers although there is indirect evidence. In the peripheral taste system, gustatory nerve fibers may selectively contact with taste receptor cells that have similar response properties and convey constant taste information to the higher order neurons.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 4","pages":"233-42"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26597859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of ATP-gated P2X3 receptors in rat gustatory papillae and taste buds.","authors":"Shinji Kataoka,&nbsp;Takashi Toyono,&nbsp;Yuji Seta,&nbsp;Kuniaki Toyoshima","doi":"10.1679/aohc.69.281","DOIUrl":"https://doi.org/10.1679/aohc.69.281","url":null,"abstract":"<p><p>It has recently become evident that ATP and other extracellular nucleotides could play an important role in signal transductions. ATP mediates excitatory signaling by means of P2X receptors. P2X3, one of its subtypes, a membrane ligand-gated ion channel, is strongly expressed in peripheral sensory neurons. The aim of the present study was to examine the distribution of nerve fibers expressing P2X3 receptors in taste buds in the gustatory papillae and soft palate of rats by immunohistochemistry. We found that the fluorescence ATP marker quinacrine stained subsets of taste bud cells. Numerous nerve fibers innervating taste buds were intensely immunostained with the P2X3 receptor antibody. These nerve fibers ascended among intragemmal cells and terminated just below the taste pores. In order to examine whether P2X3 receptors are involved in signal modulation within taste buds, we used fluorescent double stainings to analyze the distribution of P2X3 receptors and their relationship to alpha-gustducin immunopositive taste receptor cells. Many varicose nerve fibers expressing P2X3 receptor-immunoreactivities were entangled with alpha-gustducin-immunopositive taste receptor cells and ended closely below the taste pores. In fungiform papillae, nerve fibers expressing both P2X3 receptors and PGP 9.5 were observed. In contrast, only PGP 9.5 immunoreactive nerve fibers were recognized in filiform papillae. These results suggest that P2X3 receptors might be involved in taste transmission pathways within taste buds. ATP may act as a neurotransmitter, co-transmitter, or neuromodulator at P2X3 receptors to generate activating gustatory nerve fibers.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 4","pages":"281-8"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26597863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro adipocytic conversion in Meckel's chondrocytes in response to a fatty acid-containing medium.","authors":"Kiyoto Ishizeki,&nbsp;Tadayoshi Kagiya,&nbsp;Naoki Fujiwara,&nbsp;Hidemitsu Harada","doi":"10.1679/aohc.69.163","DOIUrl":"https://doi.org/10.1679/aohc.69.163","url":null,"abstract":"<p><p>Chick serum (CKS) contains factors that stimulate adipocytes in Meckel's chondrocytes in vitro. In the present study, we analyzed levels of fatty acids in CKS, and further examined whether these had the potential to convert chondrocytes to adipocytes. Phenotypic changes were evaluated by light and electron microscopies, bromodeoxyuridine (BrdU) incorporation, triglyceride assays, and immunocytochemistry. We showed that CKS contained high levels of fatty acids, and a mixed medium containing 5 particular fatty acids inhibited DNA synthesis and the proliferation of chondrocytes as it facilitated their differentiation into adipocytes. The adipocytes produced were sudan-positive multilocular cells that morphologically and histochemically resembled adipocytes induced by the CKS-containing medium. Almost all lipid droplet-containing cells were positive for leptin and alpha-glycerophosphate dehydrogenase (GPDH), as evaluated by immunoperoxidase staining, and their triglyceride concentrations markedly increased during 4 to 6 days of culture. These results suggested that specific fatty acids in CKS are involved in the adipocytic conversion of Meckel's chondrocytes.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 3","pages":"163-71"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26356315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sprouting of sensory neurons in dorsal root ganglia after transection of peripheral nerves.","authors":"Hideki Tsuyoshi,&nbsp;Keiji Zenzai,&nbsp;Haruo Okado,&nbsp;Naoto Endo,&nbsp;Minoru Shibata,&nbsp;Shigeki Hirano","doi":"10.1679/aohc.69.173","DOIUrl":"https://doi.org/10.1679/aohc.69.173","url":null,"abstract":"<p><p>Morphological reaction of sensory neurons of dorsal root ganglia after peripheral nerve transection was investigated by a nerve tracing method using E. coli lacZ (beta-galactosidase) gene recombinant adenovirus. The sciatic nerve of the rat was transected and inoculated with the gene recombinant adenovirus from the cutting end of nerve fibers. The fixation was accomplished from one to six weeks after inoculation. A whole mount specimen was observed after the reaction in a X-galactocidase substrate. Newly formed sprouting processes of dorsal root ganglion (DRG) cells appeared, all of them sprouting from the primary segment of DRG cells. Developed branches were morphologically categorized in to two types: one was the \"linear type\" which showed diverged branches running straightly along the major axis of the DRG; the other was the \"winding type\" which exhibited a random running pattern to the original axons and wound and extended in all directions in dorsal root ganglia with many branches. Many of this type encircled other cell bodies and formed a ring-like structure. There was no difference in the size of cell bodies in either type or between the ring-like structure forming the cells and those cells encircled by them.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 3","pages":"173-9"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26356316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of periodic acid-Schiff fluorescence emission for immunohistochemistry of living mouse renal glomeruli by an \"in vivo cryotechnique\".","authors":"Zilong Li,&nbsp;Nobuhiko Ohno,&nbsp;Nobuo Terada,&nbsp;Daoyuan Zhou,&nbsp;Ashio Yoshimura,&nbsp;Shinichi Ohno","doi":"10.1679/aohc.69.147","DOIUrl":"https://doi.org/10.1679/aohc.69.147","url":null,"abstract":"<p><p>To identify the distribution of endogenous serum proteins in living mouse renal glomeruli under various hemodynamic conditions, we used the periodic acid-Schiff (PAS) and its fluorescence emission as a marker for the glomerular basement membrane (GBM). The immunostaining for collagen type IV was hardly observed without microwave treatment in specimens prepared by an \"in vivo cryotechnique\". However, PAS staining and its fluorescence emission could be clearly visualized at the GBM with the \"in vivo cryotechnique\". Under normotensive conditions, immunoreaction products of albumin and immunoglobulin G heavy and light chains (IgG(H+L)) were localized within glomerular capillary loops (GCL) but not colocalized with the PAS fluorescence emission of the GBM. Under heart-arrest conditions and with quick-freezing of resected tissues, albumin, IgG (H+L), immunoglobulin kappa light chain, and IgG1 heavy chain (IgG1) were immunolocalized within the GCL and mesangial areas, but only albumin and the kappa light chain were additionally immunolocalized in Bowman's space, indicating their passage through the GBM. Under acute hypertensive conditions, both albumin and the kappa light chain, but not IgG1, were clearly immunolocalized along the GBM and in the Bowman's space, indicating their increased passage through the GBM. The overlapping areas of PAS fluorescence emission and the albumin or kappa light chain appeared to be larger with quick-freezing and under the heart arrest or acute hypertensive conditions than under normal circulation, whereas those of PAS emission and IgG1 did not differ among these conditions. The serum proteins passing through the GBM were clearly visualized with the \"in vivo cryotechnique\", immunofluorescence staining, and PAS fluorescence emission.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 3","pages":"147-61"},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26356314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytochemical characterization of glycoconjugates in the apocrine glands of the equine scrotal skin.","authors":"Tadashi Yasui,&nbsp;Azuma Tsukise,&nbsp;Takahiro Miura,&nbsp;Kousuke Fukui,&nbsp;Wilfried Meyer","doi":"10.1679/aohc.69.109","DOIUrl":"https://doi.org/10.1679/aohc.69.109","url":null,"abstract":"<p><p>Cytochemistry of glycoconjugates in the apocrine glands in the scrotal skin of the horse was studied using cytochemical methods for electron microscopy, particularly lectin cytochemistry. The secretory cells possessed a variable number of secretory vesicles, a well-developed Golgi apparatus, and abundant cisternae of the rough endoplasmic reticulum. Additionally, the basolateral plasma membrane formed numerous interdigitating folds. Glycoconjugates with vicinal diol groupings were present predominantly in the secretory vesicles, the Golgi apparatus, the surface coat of the plasma membrane, and the majority of the intracellular membranes. With lectin cytochemistry, the secretory vesicles of the glandular cells exhibited glycoproteins with different terminal sugars (alpha-D-mannose, beta-D-galactose beta-N-acetyl-D-glucosamine, and sialic acid). Several sugars were distinctly prominent in the surface coat of the plasma membrane of the secretory cells. The cytochemical properties of the complex glycoconjugates found are discussed in relation to the specific functions of the glandular secretions. These glands may have an important role in not only thermoregulation but protection of the scrotal skin, a specific body region.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 2","pages":"109-17"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26125887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of N-cadherin and E-cadherin in normal human tissues.","authors":"Benio Tsuchiya,&nbsp;Yuichi Sato,&nbsp;Toru Kameya,&nbsp;Isao Okayasu,&nbsp;Kiyoshi Mukai","doi":"10.1679/aohc.69.135","DOIUrl":"https://doi.org/10.1679/aohc.69.135","url":null,"abstract":"<p><p>E-cadherin, which expressed in various epithelial tissues, is important for the maintenance of normal epithelial phenotypes. However, the distribution of N-cadherin in normal human tissues has not been defined systemically. In the present study, we employed a sensitive, reliable immunohistochemical detection system for N-cadherin on formalin-fixed, paraffin-embedded tissue sections, and succeeded in demonstrating N- and E-cadherin protein expressions and their distribution in normal human tissues. E-cadherin immunoreactivity was detected in all the epithelial tissues examined, except for the adrenal cortical cells and granulosa cells. N-cadherin was selectively expressed on epithelial cells of the thymus, pituitary, pancreas, liver, adrenal, endometrium of the uterus, ovary, and stomach as well as in neuronal tissues. Double immunostaining revealed that N-cadherin expression was closely associated with the hormone-producing ability of cells in the pancreas and pituitary. Thus, this study indicated the possibility that N-cadherin is selectively expressed in relation to hormonal regulation in some organs and plays different functions in different situations. The method presented here should prove useful for the further investigation of the N-cadherin expression and function in several disease conditions on formalin-fixed, paraffin-embedded archival tissues.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 2","pages":"135-45"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26125890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Requirement of a bone morphogenetic protein for the maintenance and stimulation of osteoblast differentiation.","authors":"Snjezana Martinovic,&nbsp;Fran Borovecki,&nbsp;Vera Miljavac,&nbsp;Veronika Kisic,&nbsp;Drazen Maticic,&nbsp;Igor Francetic,&nbsp;Slobodan Vukicevic","doi":"10.1679/aohc.69.23","DOIUrl":"https://doi.org/10.1679/aohc.69.23","url":null,"abstract":"<p><p>The requirement of a bone morphogenetic protein for the maintenance and stimulation of an osteoblast phenotype was examined using mouse MC3T3-E1 cell cultures. Cells expressed BMP-4 mRNA, which correlated with the stimulation of the osteoblast phenotype. The addition of a BMP-4 specific antibody reduced bone nodules, suggesting that BMP-4 is required for the osteogenic activity of osteoblasts in an autocrine manner. Exogenously added BMP-7 gradually decreased the expression of BMP-4 with a concurrent stimulation of the osteoblast phenotype. Exogenous BMP-7 can therefore substitute for endogenously produced BMP-4 acting as a paracrine factor on osteoblasts. The addition of 17beta estradiol decreased BMP-4 expression but initiated synthesis of BMP-6 mRNA, an endocrine signal for osteoblasts, which also substituted for the lack of endogenous BMP-4, as evidenced by normal bone nodule formation. The addition of dexamethasone and parathyroid hormone did not affect the BMP-4 expression but induced transcripts for BMP-2 and BMP-3, respectively, suggesting that their effects on bone can be in part achieved via the BMP signaling. These experiments support the requirement of a BMP for osteoblast differentiation and function, demonstrating for the first time that a BMP can functionally substitute for another BMP in an autocrine/paracrine manner or mediate a response to an endocrine action on osteoblasts.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 1","pages":"23-36"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25969901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to aluminium changes the NADPH-diaphorase/NPY pattern in the rat cerebral cortex.","authors":"L F Rodella,&nbsp;F Ricci,&nbsp;E Borsani,&nbsp;R Rezzani,&nbsp;A Stacchiotti,&nbsp;C Mariani,&nbsp;R Bianchi","doi":"10.1679/aohc.69.13","DOIUrl":"https://doi.org/10.1679/aohc.69.13","url":null,"abstract":"<p><p>Aluminium (Al) impairs the glutamate-nitric oxide-cGMP pathway and reduces the number of nitroxidergic neurons in the rat somatosensory cortex. To understand better the effect of the time of exposure, we monitored the effect of aluminium administration on nitroxidergic neurons, identified by NADPH-diaphorase (NADPH-d) or by nitric oxide synthase (NOS) staining, after 0.5, 1, 2, 3, 6 and 12 months of aluminium administration. Since neuropeptide Y (NPY) is known to be colocalised with nitric oxide synthase in cortical neurons, the aim of this work was to study the effects of Al administration on the cortical expression of NADPH-d, nNOS, and NPY. NADPH-d or NOS positive neurons were found scattered in the cortex where they constituted about 1% of all neurons. Double staining using NADPH-d and NPY showed that almost all nitroxidergic neurons were co-localised with NPY neurons (NADPH-d/NPY double stained neurons) whereas some neurons were stained only with NPY (NPY single stained neurons) ; these were more numerous than NADPH-d/NPY double stained neurons. Al significantly reduced NADPH-d and nNOS positive neurons in the cerebral cortex time dependently, with the greatest effect appearing after 3 months. Also measured was the integrated optical density (IOD) of nNOS positive neurons showing a significant decrease of NOS immunostaining even in the remaining NOS positive neurons. The double staining experiment exhibited a decrease in NADPH-d/NPY double stained neurons with an apparent increase in NPY single stained neurons; these then decreased after 6-12 months. On the whole, the results confirm that Al impairs nitroxidergic pathways time dependently; moreover, the transient increase in NPY single stained neurons from 1 to 3 months suggests that there is an intraneuronal down-regulation of NOS, without affecting neuronal viability. In addition, the decrease in the NPY system found at 6 and 12 months may indicate that Al affected nitroxidergic and NPY systems at different times.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 1","pages":"13-21"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25969900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence of antibody production in the rat cervical lymph nodes after antigen administration into the cerebrospinal fluid.","authors":"Beatriz A Walter,&nbsp;Vladimir A Valera,&nbsp;Sugata Takahashi,&nbsp;Kenjiro Matsuno,&nbsp;Tatsuo Ushiki","doi":"10.1679/aohc.69.37","DOIUrl":"https://doi.org/10.1679/aohc.69.37","url":null,"abstract":"<p><p>We previously showed histologically that, in the rat, the cerebrospinal fluid drains from the subarachnoid space along the olfactory nerves to the nasal lymphatics and empties into the superficial and deep cervical lymph nodes. The present study was performed to investigate whether these lymph nodes play a role in the immune response of the central nervous system. For this purpose, keyhole limpet hemocyanin conjugated with fluorescein isothiocyanate (KLH-FITC) was administered into the subarachnoid space of the rat brain, and the time-kinetics and location of FITC and anti-FITC antibody forming cells in the cervical lymph nodes were studied histologically and immunohistochemically. FITC fluorescence was detected in superficial and deep cervical lymph nodes as well as the subarachnoid space and the nasal mucosa 2 h after FITC-KLH injection into the subarachnoid space. The specific antibody-forming cells first appeared in both the superficial and deep cervical lymph nodes on the 4th day after antigen administration although the reaction was more intense in the deep than in the superficial cervical lymph nodes. These cells were located in the medullary cords of the cervical lymph nodes. The number of antibody forming cells increased thereafter, reached a peak around the day 6, and then declined on day 10. These findings indicate that antigens introduced in the cerebrospinal fluid are drained into the cervical lymph nodes through the nasal lymphatics and initiate the antigen-specific immune response there. Thus, the cervical lymph nodes probably act as a monitoring site for cerebrospinal fluid and play a major role in the central nervous system immune response.</p>","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":"69 1","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.69.37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25969902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}