Archives of histology and cytology最新文献_第10页

Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells. 在HeLa细胞中，GGA2的特异性缺失导致组织蛋白酶D的错误分类。

Archives of histology and cytology Pub Date : 2007-12-01 DOI: 10.1679/aohc.70.303

Tatsuhiro Hida, Hiroko Ikeda, Satoshi Kametaka, Chihiro Akazawa, Shinichi Kohsaka, Shigeyuki Ebisu, Yasuo Uchiyama, Satoshi Waguri

{"title":"Specific depletion of GGA2 causes cathepsin D missorting in HeLa cells.","authors":"Tatsuhiro Hida, Hiroko Ikeda, Satoshi Kametaka, Chihiro Akazawa, Shinichi Kohsaka, Shigeyuki Ebisu, Yasuo Uchiyama, Satoshi Waguri","doi":"10.1679/aohc.70.303","DOIUrl":"https://doi.org/10.1679/aohc.70.303","url":null,"abstract":"Three mammalian GGAs (Golgi-localized, gamma-ear-containing, ARF-binding proteins), GGA1, 2, and 3 have been implicated in the sorting of mannose 6-phosphate receptor (MPR). To investigate the distinct roles of GGA2 in lysosomal enzyme transport, we established two stable cell lines that had a reduced expression of GGA2 by RNA interference. The expression levels of GGA2 were approximately 5% of the control levels, whereas those of non-targeted GGA1 and GGA3 were not apparently reduced. The depletion of GGA2 did not cause changes in the overall distribution of GGA1, GGA3, cation-dependent MPR, or cation-independent MPR. However, the cell lines showed increased secretion of a lysosomal enzyme, cathepsin D. In addition, a moderate expression of the dominant negative VHS-GAT domain of GGA2 had no effect on the trans-Golgi network (TGN) signal of three GGAs, nor was the GGA2 signal affected by the expression of VHS-GAT domain of GGA1 or 3. These results suggest that GGA2 is recruited to the TGN independently of the other GGAs and is required for the efficient sorting of lysosomal enzymes.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27399823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The differential distribution of type IV collagen alpha chains in the subepithelial basement membrane of the human alimentary canal. IV型胶原α链在人消化道上皮下基底膜中的差异分布。

Archives of histology and cytology Pub Date : 2007-12-01 DOI: 10.1679/aohc.70.313

Hiroyuki Sato, Ichiro Naito, Ryusuke Momota, Yoshio Naomoto, Tomoki Yamatsuji, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka

{"title":"The differential distribution of type IV collagen alpha chains in the subepithelial basement membrane of the human alimentary canal.","authors":"Hiroyuki Sato, Ichiro Naito, Ryusuke Momota, Yoshio Naomoto, Tomoki Yamatsuji, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka","doi":"10.1679/aohc.70.313","DOIUrl":"https://doi.org/10.1679/aohc.70.313","url":null,"abstract":"We studied distribution patterns of type IV collagen alpha chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using alpha(IV) chain-specific monoclonal antibodies. The alpha1(IV), alpha2(IV), alpha5(IV), and alpha6(IV) chains were found in the SBM throughout the tract, indicating the localization of [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) heterotrimeric molecules. The [alpha1(IV)](2)alpha2(IV) molecule was continuously stained, while the [alpha5(IV)](2)alpha6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of alpha3(IV) and alpha4(IV) chains which combined to form the alpha3(IV)alpha4(IV)alpha5(IV) heterotrimeric molecule with alpha5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an alpha3(IV) chain and alpha4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the alpha3(IV)alpha4(IV)alpha5(IV) molecule appears to function as a selective permeability barrier and /or as a protection against chemical stress from the luminal digestive enzymes.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.313","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27399824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Anterograde labeling of the corticospinal tract in jimpy mutant mice by Di I injection into the motor cortex. 运动皮质注射Diⅰ对跳跃突变小鼠皮质脊髓束的顺行标记。

Archives of histology and cytology Pub Date : 2007-12-01 DOI: 10.1679/aohc.70.297

Riichi Shibata-Iwasaki, Hideyuki Dekimoto, Yu Katsuyama, Satoshi Kikkawa, Toshio Terashima

引用次数: 4

The distribution of proliferating cell nuclear antigen-immunoreactive cells in the pineal organ of the rainbow trout Oncorhynchus mykiss. 虹鳟松果体器官中增殖细胞核抗原免疫反应细胞的分布。

Archives of histology and cytology Pub Date : 2007-11-01 DOI: 10.1679/aohc.70.225

Yuri Omura

{"title":"The distribution of proliferating cell nuclear antigen-immunoreactive cells in the pineal organ of the rainbow trout Oncorhynchus mykiss.","authors":"Yuri Omura","doi":"10.1679/aohc.70.225","DOIUrl":"https://doi.org/10.1679/aohc.70.225","url":null,"abstract":"Cell proliferation in the pineal organ of the immature rainbow trout Oncorhynchus mykiss was investigated by immunocytochemical demonstration of the proliferating cell nuclear antigen (PCNA) together with photoreceptor-specific opsin. Numerous PCNA-immunoreactive cells were found throughout the pineal end-vesicle and stalk. Two types of PCNA-immuno-reactive cells were distinguished: intensely stained, large ovoid and round cells, and mildly stained, slender fusiform cells. The ovoid type of the former cell was found often in the apical region and the round type in the basal region of the epithelium, while the latter fusiform cells were scattered through the apical and middle regions. Occasionally, close approaches were found between the opsin-immunoreactive photoreceptor outer segments and the PCNA-immunoreactive cells, which expressed mildly stained, nuclear and cytoplasmic signals. In addition, overlaps of the opsin-immunoreactive outer segments with the BrdU-labelled cells were occasionally found within the pineal epithelium. These findings suggest that the proliferation and neurogenesis of the pineal photoreceptor cells might persist also in the adult rainbow trout, thus maintaining highly sensitive, photo-signal transduction mechanisms for melatonin synthesis.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27284032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The distribution of type IV collagen alpha chains in the mouse ovary and its correlation with follicular development. IV型胶原α链在小鼠卵巢中的分布及其与卵泡发育的关系。

Archives of histology and cytology Pub Date : 2007-11-01 DOI: 10.1679/aohc.70.243

Kazuyo Nakano, Ichiro Naito, Ryusuke Momota, Yoshikazu Sado, Haruko Hasegawa, Yoshifumi Ninomiya, Aiji Ohtsuka

{"title":"The distribution of type IV collagen alpha chains in the mouse ovary and its correlation with follicular development.","authors":"Kazuyo Nakano, Ichiro Naito, Ryusuke Momota, Yoshikazu Sado, Haruko Hasegawa, Yoshifumi Ninomiya, Aiji Ohtsuka","doi":"10.1679/aohc.70.243","DOIUrl":"https://doi.org/10.1679/aohc.70.243","url":null,"abstract":"The present study aims to identify alpha chains of type IV collagen in the basement membrane of the mouse ovarian follicle and examine their changes during follicular development using immunofluorescence microscopy with specific monoclonal antibodies. The basement membrane of the serous mesothelium enveloping the ovary contained all alpha chains of type IV collagen, alpha1(IV) through alpha6(IV) chains. Primordial follicles showed a distinct immunoreactivity against all six alpha chains in their basement membranes. Immunolabeling for alpha3(IV) and alpha4(IV) chains was almost eliminated in the primary follicles. In basement membranes of secondary and Graafian follicles, the immunofluorescent reaction of alpha3(IV) and alpha4(IV) chains disappeared in Graafian follicles, a partial reduction in fluorescent immunostaining intensity to alpha5(IV) and alpha6(IV) chains was observed; only alpha1(IV) and alpha2(IV) chains were not degraded throughout follicular development. On atretic follicles, in addition to alpha1(IV) and alpha2(IV) chains, alpha3(IV), alpha4(IV), alpha5(IV) and alpha6(IV) chains frequently persisted. A basement membrane-like matrix within the follicular granulosa cell layer, such as the focimatrix (focal intraepithelial matrix) and/or Call-Exner body, was also recognized in mouse secondary and Graafian follicles and contained alpha1(IV), alpha2(IV), alpha5(IV) and alpha6(IV) chains but not alpha3(IV) and alpha4(IV) chains. We expect that the decrease in alpha(IV) chains prompts follicular development and is a prerequisite condition for follicular maturation.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27284035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Distinct morphology of serotonin-containing enterochromaffin (EC) cells in the rat distal colon. 大鼠结肠远端含5 -羟色胺肠色素(EC)细胞的不同形态。

Archives of histology and cytology Pub Date : 2007-11-01 DOI: 10.1679/aohc.70.235

Hirofumi Kuramoto, Makoto Kadowaki, Hiroshi Sakamoto, Kanako Yuasa, Arei Todo, Ryo Shirai

{"title":"Distinct morphology of serotonin-containing enterochromaffin (EC) cells in the rat distal colon.","authors":"Hirofumi Kuramoto, Makoto Kadowaki, Hiroshi Sakamoto, Kanako Yuasa, Arei Todo, Ryo Shirai","doi":"10.1679/aohc.70.235","DOIUrl":"https://doi.org/10.1679/aohc.70.235","url":null,"abstract":"The present study was performed to examine the distribution and distinct morphology of the serotonin-containing enterochromaffin (EC) cells in the rat distal colon by immunohistochemical and electron microscopic methods. Serotonin-immunohistochemistry revealed that most of the serotonin-immunoreactive EC cells possessed extended cytoplasmic processes. In particular, the immunoreactive EC cells with long processes located along the body of the crypt were characterized by their bipolar processes comprising one with the terminal swellings extending vertically down to the basal crypt and the other running up along the luminal side - in many cases, with the apical ends reaching the glandular lumen. Moreover, a few EC cells had long processes which resembled neuronal processes with varicosities. Electron microscopic observations revealed rod-like, tortuous, oval, or round small pleomorphic granules in the long processbearing EC cells. The cell bodies and processes directly faced the crypt epithelial cells - including the enterocytes and goblet cells on one side and the basement membrane on the opposite side. The accumulation of the granules sometimes appeared within the cytoplasm on the side of the epithelial cells. These findings suggest that serotonin is released from the long processes of the EC cells and directly acts in a paracrine fashion on the crypt epithelial cells to secrete electrolytes and fluids into the colonic lumen. The long cytoplasmic processes of the EC cells may be a major contributor to the serotonininduced secretory events in the rat distal colon.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27284033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Immunohistochemical identification of cells expressing steroidogenic enzymes cytochrome P450scc and P450 aromatase in taste buds of rat circumvallate papillae. 大鼠周围乳头味蕾中表达甾体生成酶细胞色素P450scc和P450芳香化酶细胞的免疫组织化学鉴定。

Archives of histology and cytology Pub Date : 2007-11-01 DOI: 10.1679/aohc.70.215

Kuniaki Toyoshima, Yuji Seta, Takashi Toyono, Shinji Kataoka

{"title":"Immunohistochemical identification of cells expressing steroidogenic enzymes cytochrome P450scc and P450 aromatase in taste buds of rat circumvallate papillae.","authors":"Kuniaki Toyoshima, Yuji Seta, Takashi Toyono, Shinji Kataoka","doi":"10.1679/aohc.70.215","DOIUrl":"https://doi.org/10.1679/aohc.70.215","url":null,"abstract":"The present study demonstrated for the first time the localizations and patterns of expression of key enzymes for steroidogenesis, cytochrome P450 side-chain-cleavage (P450scc), and P450 aromatase in the taste buds of rat circumvallate papillae, using immunoblot analyses and immunohistochemistry. Immunoblot analyses showed that proteins with a molecular weight close to that of rat adrenal cytochrome P450scc and a molecular weight close to that of rat ovary cytochrome P450 aromatase were present in the rat circumvallate papillae. In immunohistochemistry, antibodies against cytochrome P450scc and P450 aromatase yielded the labelings of a subset of taste bud cells. In the double immunolabeling of P450scc and alpha-gustducin or phospholipase C beta2(PLCbeta2), which were considered as markers of a majority of type II cells, P450scc was co-expressed in a subset of alpha-gustducin or PLCbeta2, but did not co-express neural adhesion molecule (NCAM), a marker of major type III cells. Further double immunolabeled studies showed that P450 aromatase was co-expressed in a subset of alpha-gustducin or PLCbeta2, but did not co-express PGP9.5, a marker of a majority of type III cells. The selective localization of cytochrome P450scc and P450 aromatase strongly suggests that estrogen biosynthesis from cholesterol might occur in a subset of type II cells of the rat taste buds. Although the full significance of estrogen in the taste bud function is not yet understand, estrogen appears to be an important regulator of taste transduction, as is the case with ATP (Finger et al., 2005), which further supports the centrality of taste cells in the life of taste buds.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27284132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The distributions of type IV collagen alpha chains in basement membranes of human epidermis and skin appendages. IV型胶原α链在人表皮和皮肤附属物基底膜中的分布。

Archives of histology and cytology Pub Date : 2007-11-01 DOI: 10.1679/aohc.70.255

Haruko Hasegawa, Ichiro Naito, Kazuyo Nakano, Ryusuke Momota, Keiichiro Nishida, Takehito Taguchi, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka

{"title":"The distributions of type IV collagen alpha chains in basement membranes of human epidermis and skin appendages.","authors":"Haruko Hasegawa, Ichiro Naito, Kazuyo Nakano, Ryusuke Momota, Keiichiro Nishida, Takehito Taguchi, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka","doi":"10.1679/aohc.70.255","DOIUrl":"https://doi.org/10.1679/aohc.70.255","url":null,"abstract":"Distributions of type IV collagen alpha chains in the basement membrane (BM) of human skin and its appendages were analyzed by immunofluorescent microscopy using chain-specific monoclonal antibodies. The basement membrane beneath the epidermis contained [alpha1(IV)](2)alpha2(IV) and [alpha5(IV)](2)alpha6(IV) but no alpha3(IV)alpha4(IV)alpha5(IV); this held true for at the eccrine sweat glands and glandular ducts, sebaceous glands, hair follicles, and arrector muscles of hair. The secretary portion of the eccrine sweat glands was rich in [alpha1(IV)](2) alpha2(IV) and had less [alpha5(IV)](2)alpha6(IV), while [alpha5(IV)](2) alpha6(IV) was abundant in the ductal portion. In the subepidermal zone, alpha5(IV)/alpha6(IV) chain negative spots (1.9-15.0 microm) were frequently observed. Triple staining samples (Mel.2, alpha2(IV) and alpha5(IV) chains) showed that about 50% of epidermal melanocytes colocalized with such spots. Results suggest that these alpha5(IV)/alpha6(IV) chain negative spots of the subepidermal basement membrane have a particular relationship with melanocytes and are sites for certain interactions between the two.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27284036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Hypothermic, but not normothermic, ischemia causes a drastic increase in cyclooxygenase-2 immunoreactive granule cells in rat dentate gyrus after 4 hours of ischemic reperfusion. 在低温而非常温下，缺血再灌注4小时后，大鼠齿状回环氧化酶-2免疫反应性颗粒细胞急剧增加。

Archives of histology and cytology Pub Date : 2007-10-01 DOI: 10.1679/aohc.70.197

Anzu Yamashita, Teruhito Kunimatsu, Toshiharu Yamamoto, Kazu-Ichi Yoshida

{"title":"Hypothermic, but not normothermic, ischemia causes a drastic increase in cyclooxygenase-2 immunoreactive granule cells in rat dentate gyrus after 4 hours of ischemic reperfusion.","authors":"Anzu Yamashita, Teruhito Kunimatsu, Toshiharu Yamamoto, Kazu-Ichi Yoshida","doi":"10.1679/aohc.70.197","DOIUrl":"https://doi.org/10.1679/aohc.70.197","url":null,"abstract":"We investigated cyclooxygenase-2 (Cox-2) immunoreactive cells in the hippocampus and dentate gyrus of Sprague-Dawley rats at 4 h after the induction of normothermic and hypothermic ischemia and reperfusion. Under the normothermic condition, Cox-2 immunoreactive cells showed more intense staining and clearer proximal dendrite configurations as compared with the control animals, whereas the numbers of immunoreactive cells in the hippocampus and dentate gyrus were not remarkably increased. In contrast to the normothermic condition, long-term (pre- and intra-ischemic) and short-term (exclusively intra-ischemic) hypothermic conditions caused a drastic increase in immunoreactive cells in the dentate gyrus. Nearly all granule cells were immuno-positive for Cox-2, whereas the CA3 and hilus sectors showed no remarkable increase in immunoreactive cell numbers. In sham-operated animals exposed to long-term hypothermia - but not ischemia, Cox-2 staining profiles were similar to those in the control animals. These results suggest that, for a drastic increase in Cox-2 immunoreactive granule cells to occur within a short time period (4 h), at a minimum, both hypothermia and ischemia, are required. Considering the neuroprotective roles of the hypothermia, a rapid increase in Cox-2 in the dentate gyrus might be associated with this temperature-sensitive phenomenon.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27117299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Interstitial cells of Cajal in the gastrointestinal musculature of W mutant mice. W突变小鼠胃肠道肌肉组织Cajal间质细胞。

Archives of histology and cytology Pub Date : 2007-10-01 DOI: 10.1679/aohc.70.163

Satoshi Iino, Satomi Horiguchi, Kazuhide Horiguchi, Yoshiaki Nojyo

{"title":"Interstitial cells of Cajal in the gastrointestinal musculature of W mutant mice.","authors":"Satoshi Iino, Satomi Horiguchi, Kazuhide Horiguchi, Yoshiaki Nojyo","doi":"10.1679/aohc.70.163","DOIUrl":"https://doi.org/10.1679/aohc.70.163","url":null,"abstract":"Interstitial cells of Cajal (ICC) are important regulatory cells generating electrical rhythmicity and transducing neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and can be examined morphologically using the c-Kit antibody. The c-kit gene is allelic with the murine white-spotting locus W, and the c-kit mutation (W mutation) affects various aspects of hematopoietic cells, germ cells, melanocytes, mast cells, and ICC. Heterozygous W/W( v) mutant mice lack a specific type of ICC and have been used to reveal its function. To search for a new model that lacks a specific type of ICC, we examined homozygous W( v)/W( v) black-eyed-white mice that are viable with anemia. Results showed the principal patterns of ICC deficiency were the same between the W/W( v) and W( v)/W( v) mutants. In the stomach of both mice, intramuscular ICC (ICC-IM) were missing and myenteric ICC (ICC-MY) were reduced in number. In the small intestine, the number of ICC-MY was severely reduced in spite of a normal distribution of deep muscular plexus ICC (ICC-DMP). The cecum also exhibited fewer reduced. ICC-IM in the colon were almost entirely missing, whereas ICC-MY were reduced only in the distal colon. In the small intestine and colon, the number of remaining ICC-MY in W( v)/W( v) mice was greater than that in W/W( v) mice. The enteric nervous system of the two mutant mice showed normal characteristics. From these findings, we conclude that W( v)/W( v) mice represent a new genotype that lacks a part of the ICC in its gastrointestinal musculature.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/aohc.70.163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27117385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35