Archives of histology and cytology最新文献

Immunohistochemical characterization of interstitial cells of Cajal (ICC) in the mouse intestine using whole mount preparations – with particular reference to their density, morphological features, and network patterns 小鼠肠道Cajal (ICC)间质细胞的免疫组织化学表征-特别是它们的密度、形态特征和网络模式

Archives of histology and cytology Pub Date : 2020-05-01 DOI: 10.1679/aohc.80.1

Shoko Kawashima, Sachiko Miyamoto-Kikuta, T. Ezaki, Takahiro Okamoto

引用次数: 0

Phosphorylation and dephosphorylation of aquaporin-2 at serine 269 and its subcellular distribution during vasopressin-induced exocytosis and subsequent endocytosis in the rat kidney 水通道蛋白-2在丝氨酸269的磷酸化和去磷酸化及其在血管加压素诱导的大鼠肾脏胞吐和随后的内吞过程中的亚细胞分布

Archives of histology and cytology Pub Date : 2017-10-26 DOI: 10.1679/AOHC.77.25

Kinue Shimizu, Megumi Sano, Aoi Kita, N. Sawai, A. Iizuka-Kogo, H. Kogo, T. Aoki, K. Takata, T. Matsuzaki

{"title":"Phosphorylation and dephosphorylation of aquaporin-2 at serine 269 and its subcellular distribution during vasopressin-induced exocytosis and subsequent endocytosis in the rat kidney","authors":"Kinue Shimizu, Megumi Sano, Aoi Kita, N. Sawai, A. Iizuka-Kogo, H. Kogo, T. Aoki, K. Takata, T. Matsuzaki","doi":"10.1679/AOHC.77.25","DOIUrl":"https://doi.org/10.1679/AOHC.77.25","url":null,"abstract":"Aquaporin-2 (AQP2) is a water channel protein that is trafficked between intracellular vesicles and the plasma membrane of kidney collecting duct cells upon vasopressin stimulation. Vasopressin changes the phosphorylation states of the AQP2 C-terminal serines (Sers), Ser256, Ser261, Ser264, and Ser269, in rats and mice, which is thought to play a role in controlling trafficking. Here, we focused on Ser269. We developed a specific antibody to Ser269-phosphorylated AQP2. Using immunofluorescence microscopy, we examined its localization in the rat kidney following injection of vasopressin and a vasopressin type 2 receptor-specific antagonist (OPC-31260). Ser269-phosphorylated AQP2 was almost undetectable in the water-loaded rat kidney, but was detected intracellularly soon after vasopressin injection, and then highly accumulated on the apical membrane of connecting tubule and collecting duct principal cells. In addition to the apical membrane, Ser269phosphorylated AQP2 was also detected on the basolateral membrane of connecting tubule cells and inner medullary collecting duct principal cells. OPC-31260 injection following vasopressin stimulation caused internalization of AQP2, a pool of which was phosphorylated at Ser269. These results suggest that 1) AQP2 is phosphorylated at Ser269 intracellularly upon vasopressin stimulation and is rapidly trafficked to the plasma membrane, and 2) AQP2 can be internalized from the plasma membrane even if it remains phosphorylated at Ser269.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45234387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Induction of Autophagy in the Hippocampus after Hypoxic Ischemic Injury to Neonatal Rats 新生大鼠缺氧缺血性损伤后海马自噬的诱导

Archives of histology and cytology Pub Date : 2017-03-03 DOI: 10.1679/AOHC.77.13

M. Koike, Ai Kawahara, M. Shibata, Y. Uchiyama

{"title":"Induction of Autophagy in the Hippocampus after Hypoxic Ischemic Injury to Neonatal Rats","authors":"M. Koike, Ai Kawahara, M. Shibata, Y. Uchiyama","doi":"10.1679/AOHC.77.13","DOIUrl":"https://doi.org/10.1679/AOHC.77.13","url":null,"abstract":"Summary. Neonatal hypoxic/ischemic (H/I) brain injury causes neurological impairment, including cognitive and motor dysfunction as well as seizures. Patterns of H/I injury-induced neuron death using rodent models are considered to be similar to the cases in human H/I encephalopathy. The participation of autophagy in neuron death has been a common feature in neonatal rodent models of H/I brain injury and human H/I encephalopathy when examined by immunochemical approaches for MAP1-LC3. This tendency has also been confirmed in neuronal tissue-specific Atg7 conditional knockout mice. However, while the current rat H/I model that is used for analyzing autophagy results in global damage to the ipsilateral hemisphere, it does not entirely reflect the neuropathological changes that appear in the neonatal mouse H/I model, in which the hippocampus is selectively damaged. The present study established a neonatal rat model of H/I injury with a milder ischemic insult, in which autophagy was involved in the hippocampal CA1 region after H/I injury when examined by electron microscopy, and by immunohistochemical and biochemical analyses of LC3.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.77.13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43845886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vascular architecture of the carotid labyrinth in larval and adult Xenopus laevis – Histomorphology and scanning electron microscopy of vascular corrosion casts 非洲爪蟾幼虫和成虫颈动脉迷宫的血管结构。血管腐蚀模型的组织形态学和扫描电镜

Archives of histology and cytology Pub Date : 2017-03-03 DOI: 10.1679/AOHC.77.1

C. Deutschmann, B. Minnich, A. Lametschwandtner

{"title":"Vascular architecture of the carotid labyrinth in larval and adult Xenopus laevis – Histomorphology and scanning electron microscopy of vascular corrosion casts","authors":"C. Deutschmann, B. Minnich, A. Lametschwandtner","doi":"10.1679/AOHC.77.1","DOIUrl":"https://doi.org/10.1679/AOHC.77.1","url":null,"abstract":"In Xenopus laevis Daudin the anlage of the carotid labyrinth, a baroand chemoreceptor, appears in early premetamorphosis (NF stage 53) as a swelling of the third aortic arch at the point where the common carotid artery gives off the external carotid artery. A conspicuous accumulation of melanocytes preceeds the formation of the labyrinth maze which starts to grow at the end of metamorphic climax (NF stage 64) and continues so through juvenile age. In adult animals the carotid labyrinth displays a spherical to ovoid shape, has an average length of 1.4 mm, a width of about 0.8 mm and a height of about 0.4 mm. The maze vessels form by sprouting angiogenesis from the common carotid artery and the internal carotid artery. Scanning electron microscopy of vascular corrosion casts reveals that size, overall shape, surface morphology and vascular patterns of the carotid labyrinth vary greatly between individuals, whereby intussusceptive microvascular growth (= nonsprouting angiogenesis) and its facets model and contribute to the maturation of the labyrinth’s final vascular patterns.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43686896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of ATP-sensitive K + channel subunits in rat pituitary gland 大鼠垂体atp敏感K +通道亚基的定位

Archives of histology and cytology Pub Date : 2016-06-22 DOI: 10.1679/AOHC.76.53

M. Zhou, R. Suzuki, A. Ishizawa, O. Tanaka, Y. Yasuoka, Yoshinori Kanatsu, K. Kawahara, H. Abe

{"title":"Localization of ATP-sensitive K + channel subunits in rat pituitary gland","authors":"M. Zhou, R. Suzuki, A. Ishizawa, O. Tanaka, Y. Yasuoka, Yoshinori Kanatsu, K. Kawahara, H. Abe","doi":"10.1679/AOHC.76.53","DOIUrl":"https://doi.org/10.1679/AOHC.76.53","url":null,"abstract":"ATP-sensitive K (KATP) channel subunits Kir6.1, Kir6.2, SUR1, SUR2A, and SUR2B in the rat pituitary gland were first investigated by RT-PCR assay and immunohistochemical staining. The results of RTPCR analysis showed that the rat pituitary gland expressed the five KATP channel subunits mentioned above. Immunohistochemical staining showed that these KATP channel subunits were widely localized in the anterior lobe, intermediate lobe, and posterior lobe at different intensities. Immunofluorescence double and triple staining showed that these KATP channel subunits colocalized with cells containing adrenocorticotropic hormone (ACTH) in the anterior lobe of the pituitary gland. Interestingly, neither Kir6.1 nor Kir6.2 colocalized with cells containing prolactin (PRL), follicular stimulating hormone (FSH), and growth hormone (GH). These results suggest that ACTH cells contain four types of KATP channels: Kir6.1/SUR2A, Kir6.2/SUR2A, Kir6.1/ SUR2B, and Kir6.2/SUR2B. KATP channels may play some important roles in ACTH cells in the pituitary gland. The different compositions of KATP channel subunits in corticotrophs but not in the PRL, FSH, and GH cells might be due to the different metabolic situations of these cells.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.76.53","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Microvascularization of the Pancreas in Larval and Adult Xenopus laevis – Histomorphology and Scanning Electron Microscopy of Vascular Corrosion Casts 非洲爪蟾幼体和成年体胰腺微血管的形成——血管腐蚀模型的组织形态学和扫描电镜

Archives of histology and cytology Pub Date : 2016-04-04 DOI: 10.1679/AOHC.76.35

J. Höpner, A. Lametschwandtner

{"title":"Microvascularization of the Pancreas in Larval and Adult Xenopus laevis – Histomorphology and Scanning Electron Microscopy of Vascular Corrosion Casts","authors":"J. Höpner, A. Lametschwandtner","doi":"10.1679/AOHC.76.35","DOIUrl":"https://doi.org/10.1679/AOHC.76.35","url":null,"abstract":"The microvascularization of the pancreas of larval and adult South African Clawed Toads Xenopus laevis, was studied by scanning electron microscopy of vascular corrosion casts and light microscopy of paraplast embedded Goldner-stained serial tissue sections. We showed that branches of left and right gastric artery, hepatic artery and anterior intestinal artery, namely anterior pancreatic, anterior middle pancreatic, posterior middle pancreatic and caudal pancreatic arteries supply and pancreatic veins drain the adult pancreas into hepatic portal vein, anterior and middle gastric vein, gastroduodenal vein, and anterior duodenal vein. In premetamorphosis the pancreas showed a dense but immature vascular bed with signs of ongoing sprouting and non-sprouting angiogenesis (=intussusceptive microvascular growth; IMG). During metamorphic climax the pancreas shrinked dramatically paralleled by vascular regression. The larval pancreas had an underdeveloped ductal system which in the course of pancreas remodeling during metamorphic climax developed into a complex ductal system. In adult Xenopus laevis the pancreas showed intralobular islets of Langerhans only and an insuloacinar portal vessel system as described sofar in reptiles, birds and mammals. Islets in Xenopus located superficially and within deeper regions and emitted both insulo-acinar portal vessels and insulo-venous efferent vessels. Intrainsular microvascular patterns found suggest that in Xenopus islets blood flows first to β-cells and subsequently to the other endocrine cells present in the endocrine pancreas.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.76.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Localization of diacylglycerol kinase ζ in rat pancreatic islet cells under normal and streptozotocin-induced stress conditions 正常和链脲佐菌素诱导应激条件下大鼠胰岛细胞中二酰基甘油激酶ζ的定位

Archives of histology and cytology Pub Date : 2016-03-03 DOI: 10.1679/AOHC.76.23

Y. Hozumi, T. Nakano, Toshiaki Tanaka, K. Goto

{"title":"Localization of diacylglycerol kinase ζ in rat pancreatic islet cells under normal and streptozotocin-induced stress conditions","authors":"Y. Hozumi, T. Nakano, Toshiaki Tanaka, K. Goto","doi":"10.1679/AOHC.76.23","DOIUrl":"https://doi.org/10.1679/AOHC.76.23","url":null,"abstract":"Summary. The pancreas comprises exocrine and endocrine portions, the latter of which is a glucose-responsive tissue that secretes hormones in response to serum glucose levels. One pathway implicated in the regulatory mechanism of this gland is the phosphoinositide (PI) cycle, which generates second messengers. Diacylglycerol (DG), the major second messenger in the PI signaling cascade, is catalyzed by the diacylglycerol kinase (DGK) family. We previously described characteristic expression and localization patterns of DGKs in various organs under pathophysiological conditions. Nevertheless, little is known about the characteristics and morphological aspects of this enzyme family in the pancreas. This study was conducted to investigate the pancreas, specifically the expression and localization of the DGK family. RT-PCR analysis reveals that DGKζ is the major isozyme in the pancreas. Additionally, we show that DGKζ is expressed in pancreatic islet cells, but not in the exocrine cells. It localizes predominantly to the nuclei of α-, β-, and δ-cells. We found further that DGKζ translocates from the nucleus to the cytoplasm in β-cells in response to a β-cell-selective toxin streptozotocin (STZ) and that it disappears over time. These findings will substantiate and extend our understanding of the functional roles of DGKζ in pancreatic islet cells.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.76.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pivotal role of duct epithelia in salivary gland GVHD 唾液腺GVHD中导管上皮的关键作用

Archives of histology and cytology Pub Date : 2016-03-03 DOI: 10.1679/AOHC.76.9

Masaru Sasaki, Y. Sawanobori, Toshiya Tanaka, Hiashi Ueta, Y. Kitazawa, Sachiko Kikuta, T. Issekutz, K. Matsuno

引用次数: 1

A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney 在大鼠肾脏中，丙氨酰氨基肽酶/氨基肽酶N/CD13可有效水解三肽酶活性的特异性三肽基底物

Archives of histology and cytology Pub Date : 2016-03-03 DOI: 10.1679/AOHC.76.1

M. Shibata, M. Koike, S. Kusumi, Noboru Sato, Y. Uchiyama

{"title":"A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney","authors":"M. Shibata, M. Koike, S. Kusumi, Noboru Sato, Y. Uchiyama","doi":"10.1679/AOHC.76.1","DOIUrl":"https://doi.org/10.1679/AOHC.76.1","url":null,"abstract":"amino terminal amino acid sequence of the peptidase was x-Ala-Pro-x-Leu-Pro-Gly-Ser-Thr-Ser-Ala-Thr-x-x-Ser, where x indicates undetectable amino acid residues, and the antiserum against the peptidase was immunopositive for the brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi. These results indicate that the unknown peptidase that hydrolyzed AAF-MCA is the soluble form of aminopeptidase N/CD13, and caution is required when using AAF-MCA as a substrate for tripeptidyl peptidase assays. Summary. L-Alanyl-L-alanyl-L-phenylalanine 4-methyl-coumaryl-7-amide (AAF-MCA) is one of the classic substrates for use with tripeptidyl peptidases (TPP-I and TPP-II). We have previously clarified the tissue distribution of TPP-I in detail and noted that the protein expression of TPP-I is often incompatible with its enzyme activity. Herein, we describe the unknown peptidase, which could effectively hydrolyze AAF-MCA, in the rat kidney. The peptidase was purified after four chromatography steps, and its enzyme characteristics were elucidated. The peptidase activity was inhibited by amastatin, bestatin, and o-phenanthroline and was also inhibited by zinc and copper ions. The substrate specificity for several monoamino acidic-MCAs revealed that the peptidase had an affinity for alanyl-MCA. The","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.76.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Olig genes are upregulated in oligodendrocyte precursor cells in the injured central nervous system 在中枢神经系统损伤的少突胶质细胞前体细胞中寡基因上调

Archives of histology and cytology Pub Date : 2013-01-01 DOI: 10.1679/AOHC.74.1

K. Iseki, S. Hagino, Tetsuji Mori, Yuxiang Zhang, N. Sakai, S. Yokoya, Y. Hozumi, K. Goto, A. Wanaka, C. Tase

{"title":"Olig genes are upregulated in oligodendrocyte precursor cells in the injured central nervous system","authors":"K. Iseki, S. Hagino, Tetsuji Mori, Yuxiang Zhang, N. Sakai, S. Yokoya, Y. Hozumi, K. Goto, A. Wanaka, C. Tase","doi":"10.1679/AOHC.74.1","DOIUrl":"https://doi.org/10.1679/AOHC.74.1","url":null,"abstract":"The Olig gene family encodes basic helixloop-helix (bHLH) transcription factors and plays a critical role in oligodendrogenesis during development. We examined whether the Olig genes are involved in pathological conditions such as the injured brain or spinal cord by in situ hybridization. Olig1 mRNA was detected in cells surrounding necrotic tissue. The signal intensity of Olig1 mRNA gradually increased 2 to 7 days and decreased by 14 days after injury. Olig2 mRNA was expressed in a similar pattern and was colocalized with Olig1 mRNA. Similar results were obtained for the expression of Olig1 and Olig2 mRNAs in spinal cord injury. When we compared the mRNA expression pattern of Olig1 with that of NG2 proteoglycan and platelet-derived growth factor receptor α (PDGFRα), markers for oligodendrocyte precursor cells (OPCs), by double labeling in situ hybridization, Olig1 mRNA was co-localized with mRNAs for NG2 proteoglycan and PDGFRα 7 days after brain injury, suggesting that Olig mRNAs were expressed in OPCs. These findings suggest that the Olig transcription factors regulate differentiation of oligodendrocytes in the injured CNS as well as in the developing CNS.","PeriodicalId":8307,"journal":{"name":"Archives of histology and cytology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1679/AOHC.74.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0