Differential expression and localization of connexins 26 and 43 in the rat gingival epithelium.

Q4 Medicine

Archives of histology and cytology Pub Date : 2008-11-01 DOI:10.1679/aohc.71.147

Takashi Muramatsu, Tomoko Uekusa, Takayasu Masaoka, Masato Saitoh, Sadamitsu Hashimoto, Yoshihiro Abiko, Han-Sung Jung, Masaki Shimono

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引用次数: 7

Abstract

We investigated the expression and localization of connexins (CX) 26 and 43 in the rat gingival epithelium. RT-PCR analysis revealed CX26 gene expression in both the upper and lower layers of the gingival epithelium and in the total epithelial layer, whereas CX43 gene expression was limited to the lower layer and the total epithelial layer. Immunoreactivity for CX43 was observed in the membranes of adjacent cells from the basal layer to the middle of the prickle cell layer, while immunoreactivity for CX26 was observed in the granular cell layer and lower part of the squamous cell layer. Merged images revealed the co-localization of CX26 and CX43 in the middle of the prickle cell layer. By immuno-electron microscopy, gap junctions appeared curved, hemi-circular, or annular within the cytoplasm, and gold particles indicating the presence of CX43 were localized at the outer edges of these cytoplasmic formations. These results suggest that CX43 is associated with the regulation of cell proliferation and that increased CX26 expression is associated with differentiation of keratinocytes. Thus, degradation of CX43 is considered to play an essential role in differentiation of the rat gingival epithelium.

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连接蛋白26和43在大鼠牙龈上皮中的差异表达和定位。

我们研究了连接蛋白(cx26和cx43)在大鼠牙龈上皮中的表达和定位。RT-PCR分析显示，CX26基因在龈上皮上下两层及总上皮中均有表达，而CX43基因仅在龈上皮上下两层及总上皮中表达。CX43在基底层至棘细胞层中层相邻细胞的细胞膜上观察到免疫反应性，而CX26在颗粒细胞层和鳞状细胞层下层观察到免疫反应性。合并图像显示CX26和CX43在棘细胞层中间共定位。通过免疫电镜观察，细胞质内的间隙连接呈弧形、半圆形或环状，并且表明存在CX43的金颗粒定位在这些细胞质结构的外缘。这些结果表明，CX43与细胞增殖的调节有关，而CX26表达的增加与角质形成细胞的分化有关。因此，CX43的降解被认为在大鼠牙龈上皮的分化中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of histology and cytology 生物-细胞生物学

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期刊介绍： The Archives of Histology and Cytology provides prompt publication in English of original works on the histology and histochemistry of man and animals. The articles published are in principle restricted to studies on vertebrates, but investigations using invertebrates may be accepted when the intention and results present issues of common interest to vertebrate researchers. Pathological studies may also be accepted, if the observations and interpretations are deemed to contribute toward increasing knowledge of the normal features of the cells or tissues concerned. This journal will also publish reviews offering evaluations and critical interpretations of recent studies and theories.