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[Results of endocrinologic studies in culture media after coculture of bovine oocytes with granulosa cells]. [牛卵母细胞与颗粒细胞共培养后培养基中的内分泌学研究结果]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
M Götze, G G Gerassimova, H Alm, S N Makarova, F Schneider, W B Dmitriev, P Kauffold, A K Golubjev
{"title":"[Results of endocrinologic studies in culture media after coculture of bovine oocytes with granulosa cells].","authors":"M Götze,&nbsp;G G Gerassimova,&nbsp;H Alm,&nbsp;S N Makarova,&nbsp;F Schneider,&nbsp;W B Dmitriev,&nbsp;P Kauffold,&nbsp;A K Golubjev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The steroid hormones progesterone (P.), and testosterone (T.) were radio-immunologically determined in 108 medium samples, following co-culturing of bovine oocytes with granulosa cells. P. and T. values recorded from a control group were lower with significance than those recorded from co-culturing groups, that is 72 +/- 21 ng/ml and 264 +/- 84 pg/ml as compared to 208 +/- 138 ng/ml and 2,168 +/- 1,595 pg/ml in the oocyte plus fresh granulosa cell co-culturing group as well as 364 +/- 215 ng/ml and 825 +/- 233 pg/ml in the oocyte plus pre-incubated granulosa cell co-culturing group. These rises were accompanied by decline in maturation rate, increase in oocyte degeneration, and rises in the rates of fertilisation and segmentation.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"941-6"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13257202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Birth weight, parturition and meat production in the dairy cow population]. [奶牛种群的出生体重、分娩和肉类产量]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
H D Matthes, L Panicke
{"title":"[Birth weight, parturition and meat production in the dairy cow population].","authors":"H D Matthes,&nbsp;L Panicke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Birth weight was found to be closely related to calving behaviour. With all variations in Friesian dairy cattle, rising beef yield proved to be achievable even along with medium birth weight, so that calving complications and resulting decline in reproduction can be avoided.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"947-53"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13257203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[PMSG dosage precision in young and old sows in the course of biotechnical ovulation synchronization. 3. Fertility and litter effects]. 生物技术促排卵过程中幼、老母猪PMSG用量精度的研究。3.生育力和产仔效应]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
B Rubo, J Bergfeld
{"title":"[PMSG dosage precision in young and old sows in the course of biotechnical ovulation synchronization. 3. Fertility and litter effects].","authors":"B Rubo,&nbsp;J Bergfeld","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One single PMSG batch, Pregmagon-Dessau, was compared with other PMSG doses on 2,126 adult sows and 1,700 gilts from five farms. Additional comparisons were made with other Pregmagon or Prolosan batches on 1,178 adult sows and 1,026 gilts. All experiments were made in the context of a programme for ovulation synchronisation and term-oriented insemination. Doses of 700, 800, 900, and 1,000 IU were tested on adult sows and 600, 800, and 1,00 on gilts, with the mean dose of 800IU being found to be sufficient or superior to other doses for fertility results. Doses for adult sows could be adjusted and related to present and previous litter numbers, with the dosage being between 700IU (treatment for third litter) and 700 to 800IU (treatment following average to high previous litter. The preferential dose for gilts was found to be 800IU. Higher PMSG doses (up to 1,000IU) should not be used in synchronisation of ovulation and term-oriented insemination unless extraordinary conditions were safely established.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"803-12"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13258008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Model of bacteriologic effectiveness testing of disinfectants for veterinary medicine]. 兽药消毒剂细菌学有效性试验模型
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
U Kleiner, P Trenner
{"title":"[Model of bacteriologic effectiveness testing of disinfectants for veterinary medicine].","authors":"U Kleiner,&nbsp;P Trenner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Proper evaluation of effectiveness of chemical disinfectants depends on availability of a standardised test methodology which can provide reproducible values. The binding rules and regulations for testing and acceptance under the heading of \"Methods for Testing Chemical Disinfectants for Veterinary Medicine\" (team of authors, 1987) have resulted from years of experimental research in the field of disinfectant development. They include a model for bacteriological laboratory testing which is described in this paper, with complementary reference being made to experimental planning and appraisal of disinfection success. An account is given of preparatory testing, using agar diffusion and serial dilution tests, and of main testing, using suspension and microorganism carrier tests. The RF% microorganism reduction rate is introduced for appraisal of disinfection success. With differentiated initial germ counts, the comparability of experimental results provided by RF% has proved to be superior to that obtainable from conventional calculation.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 1","pages":"103-16"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13342718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The effect of steroid hormone doses after ovariectomy on the peripheral hormone processes and uterine development in gilts. 2. Effects on gilts during early pregnancy]. 卵巢切除术后类固醇激素剂量对后备母猪外周激素过程和子宫发育的影响。2. 妊娠早期对后备母猪的影响。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
B Lampe, F Schneider, K P Brüssow, G Blödow, K Wollenhaupt, K Spitschak, U Hühn
{"title":"[The effect of steroid hormone doses after ovariectomy on the peripheral hormone processes and uterine development in gilts. 2. Effects on gilts during early pregnancy].","authors":"B Lampe,&nbsp;F Schneider,&nbsp;K P Brüssow,&nbsp;G Blödow,&nbsp;K Wollenhaupt,&nbsp;K Spitschak,&nbsp;U Hühn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Experimental studies were conducted into ovariectomized pregnant gilts to establish effects of exogenic hormone administration, with endogenic ovarian steroids excluded, upon uterus and fetus development as well as on hormone levels in blood plasma, endometrium, and allantoic fluid. Hormone concentrations in blood plasma were found to depend clearly on hormone doses applied after ovariectomy to preserve pregnancy. 2 to 3 weeks of smooth gravidity, following ovariectomy, were ensured on the 6th or 14th day after KB1 by daily application of very low doses of progesterone only (80 mg) or in combination with estrogens, the ratio being 480:1.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 3","pages":"347-56"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13392425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study into wild Egyptian plants of potential medicinal activity. Ninth communication: hypoglycaemic activity of some selected plants in normal fasting and alloxanised rats. 埃及潜在药用野生植物的研究。第九通讯:一些选定植物在正常禁食和四氧化大鼠中的降糖活性。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
M M Shabana, Y W Mirhom, A A Genenah, E A Aboutabl, H A Amer
{"title":"Study into wild Egyptian plants of potential medicinal activity. Ninth communication: hypoglycaemic activity of some selected plants in normal fasting and alloxanised rats.","authors":"M M Shabana,&nbsp;Y W Mirhom,&nbsp;A A Genenah,&nbsp;E A Aboutabl,&nbsp;H A Amer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>31 desert plants belonging to 17 families were collected from different Egyptian localities. 21 plants extracts were orally given to normal rats, and 15 were tested on fasted and to alloxanised rats. The results were compared with a standard oral hypoglycaemic drug (Daonil, Hoechst) used as a positive control. The following findings were obtained: 8 plants exhibited persistent hypoglycaemic effects, Lycium shawii, Salvia (S.) aegyptiaca, Pergularia tomentosa, Convolvulus (C.) althaeoides, Haloxylon salicornicum, Ephedra alata, Scrophularia deserti, and Crotalaria aegyptiaca. Transient hypoglycaemic effects appeared only 1 hour after administration in response to 4 plants, Silena succulenta, Lygos raetam, C. lanatus, and Pulicaria incisa. In the cases of Ochradenus baccatus and Zygophyllum album, slow hypoglycaemic activity was produced and appeared 3 hours after administration. 5 plants showed hypoglycaemic effects viz, Thymus capitatus, Launaea nudicaulis, Conyza dioscorides, Nitraria retusa, and Limonium tubiflorum. Among the 15 plant extracts tested on alloxanised diabetic rats only 4 showed hypoglycaemic effects more potent than those of the administered dose of Daonil. These were Matthiola livida, S. aegyptiaca, Astragalus species, and Arthrocnemum glaucum. The hypoglycaemic effect of S. aegyptiaca in fasting rats has been confirmed also in alloxanised diabetic animals. This emphasises the importance of conducting both experiments in order to obtain a reliable conclusion.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 3","pages":"389-94"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13392429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthetic peptides against foot-and-mouth disease--immunization with VP1-peptides of type O1-Kaufbeuren. 合成抗口蹄疫肽——用vp1 - 1- kaufbeuren型肽免疫。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
H Liebermann, U Holl, I Reimann, A Nöckler, D Schäfer, G Thalmann, R Dölling
{"title":"Synthetic peptides against foot-and-mouth disease--immunization with VP1-peptides of type O1-Kaufbeuren.","authors":"H Liebermann,&nbsp;U Holl,&nbsp;I Reimann,&nbsp;A Nöckler,&nbsp;D Schäfer,&nbsp;G Thalmann,&nbsp;R Dölling","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Coupled synthetic peptides, representing the sequences of amino acids 130-160, 141-160 and 145-160 of foot-and-mouth disease virus O1K protein VP1, induced virus-binding and virus-neutralizing antibody response in guinea pigs, rabbits, and pigs. We also detected antibody response in guinea pigs after immunization with uncoupled peptides and in cattle with 21 aa-peptide-Keyhole-limpet hemocyanin (-KLH). The best results were obtained from 21 aa-peptide-KLH and 31 aa-peptide with or without KLH or thyroglobulin as carrier. Our preliminary results show the induction of virus-neutralizing antibodies to be obviously influenced by length of the peptide as well as by the kind of carrier and coupling.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"883-90"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13125487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Histologic and biochemical studies of the uteri of ovariectomized gilts during the first two months of pregnancy after different hormone substitutions. 2. Effects of reducing the progesterone dosage on the survival rate of embryos and the structure of the placenta and the influence of oral administration of norgestrel]. 去卵巢后备母猪妊娠头两个月不同激素替代后子宫的组织学和生化研究。2. 减少黄体酮用量对胚胎存活率和胎盘结构的影响及口服诺孕酮的影响[j]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
U Schnurrbusch, G Otto, T Hong, E Ullrich, K Elze
{"title":"[Histologic and biochemical studies of the uteri of ovariectomized gilts during the first two months of pregnancy after different hormone substitutions. 2. Effects of reducing the progesterone dosage on the survival rate of embryos and the structure of the placenta and the influence of oral administration of norgestrel].","authors":"U Schnurrbusch,&nbsp;G Otto,&nbsp;T Hong,&nbsp;E Ullrich,&nbsp;K Elze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies were conducted into ovariectomised gilts in early gravidity, with the view to finding out if reduction of daily progesterone doses had an impact upon embryo survival rate and on the structure of the placenta and if experimentally induced progesterone deficit could be offset by oral administration of norgestrel, a synthetic progestagen. Embryo survival were found to drop from 80 to 26.1 percent in response to reduced progesterone dosage from 120 mg to 40 mg, average numbers of living embryos per animal being 9.6 or 3.0. Oral administration of 12 mg of norgestrel, in addition to injection of 40 mg of progesterone, enhanced the survival rate to 64.6 percent, the average number of living embryos coming to 8.9. Reduction of progesterone doses compared to animals with sufficient progesterone supply. Depressed the beginning decrease of the thickness endometrium and surface epithelium oedematisation of the endometrial stroma was mitigated, and subepithelial hyperaemia disappeared altogether. The secretory activity of uterine glands declined, and so did the endometrial activities of acid and alkaline phosphatases. Administration of norgestrel proved helpful in substantive removal of manifestations observed in the progesterone deficit group.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 5","pages":"685-701"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13252190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of RD114-related endogenous cat retroviral elements ECE2. 与rd114相关的内源性猫逆转录病毒元件ECE2的鉴定
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
S Prösch
{"title":"Characterization of RD114-related endogenous cat retroviral elements ECE2.","authors":"S Prösch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>RD114 virus is an endogenous xenotropic Type C retrovirus of domestic cat. Previously, it had been shown that genomic DNA of cat contained approximately 20 copies of RD114-related sequences. Only one encoded for the replication-competent RD114 virus. The endogenous sequences exhibited substantial sequence conservation within the gag and pol genes and LTRs, but were characterized by deletions and substitutions within the env region. The endogenous cat retroviral element ECE2 was isolated by screening a genomic DNA library of cat liver DNA with an env-specific cDNA of RD114 virus. It contained an env region which differed from all RD114-related sequences so far isolated and was homologous to the corresponding region of replication-competent RD114 with regard to their restriction map and partial sequence analysis (p20). Otherwise, ECE2 had a deletion of approximately 1 kbp in the putative pol gene and, therefore, did not represent the locus of inducible RD114 virus.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"935-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13257201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence of caprine herpesvirus (BHV-6) infection in goat population of the GDR. 德意志民主共和国山羊群体中山羊疱疹病毒(BHV-6)感染的发生。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1990-01-01
A Muluneh, H Liebermann
{"title":"Occurrence of caprine herpesvirus (BHV-6) infection in goat population of the GDR.","authors":"A Muluneh,&nbsp;H Liebermann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To clarify the occurrence of the caprine herpesvirus (BHV-6) infection in the goat population in the GDR, 175 sera, collected from the agricultural research station of Karl-Marx-Universität at Probstheida were tested for the presence of neutralizing antibodies against BHV-6. BHV-6 antibodies were present in 12% of the sera examined. The titer was low. Cross-neutralization of BHV-6 could not be determined with any other bovine herpesvirus. The goat sera tested were free of neutralizing antibodies against BHV-1 and BHV-3. The indirect immunofluorescence antibody test revealed cross-reactivity only between BHV-6 and BHV-1.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 2","pages":"254-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13320774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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