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Hemato-clinical changes in phenylhydrazine-induced acute hemolytic anaemia in calves. 苯肼引起的犊牛急性溶血性贫血的血液临床变化。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
M L Sharma, J L Soni, K Datta
{"title":"Hemato-clinical changes in phenylhydrazine-induced acute hemolytic anaemia in calves.","authors":"M L Sharma,&nbsp;J L Soni,&nbsp;K Datta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute anaemia was induced in calves aged 1 week, using the oxidant chemical phenylhydrazine-hydrochloride (PHH). The hematoclinical responses to graded doses were evaluated. Mild anaemia was observed with the 60 mg/kg body weight (B.W.) dose. The 100 mg/kg B.W. dose was too toxic. The sublethal 80 mg/kg B.W. dose produced the desired type of hemolytic anaemia, the peak of which being observed on d 4 post-injection (p.i.). Hemolytic anaemia was evidenced by hemoglobinaemia and hemoglobinuria. The acute toxicity symptoms lasted for about 30 min. Thereafter, the animals appeared to overcome the stress. In general, increases in pulse and respiration coincided with progress of the hemolytic syndrome. Peak response occurred 48 to 60 h p.i.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"39-43"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Selected factors influencing the in vitro maturation of oocytes of cattle]. [影响牛卵母细胞体外成熟的选定因素]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
M Böttcher, J Saedler, P Irmer, D Göbel, W Lange
{"title":"[Selected factors influencing the in vitro maturation of oocytes of cattle].","authors":"M Böttcher,&nbsp;J Saedler,&nbsp;P Irmer,&nbsp;D Göbel,&nbsp;W Lange","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies were conducted into hormonal additives to medium and culturing time and their effects on in vitro maturation of bovine oocytes. The best maturation results were ensured in vitro by substitution of TCM-199 with FSH, HCG, and 17 beta-oestradiol: The stage of fertilisation capability (telophase I or metaphase II) was reached by 86% of all oocytes (115 of 134). The rate of maturation was worse with significance in FSH-free medium (62% or 101 of 163), and the amount of degenerated oocytes was twice as high (18%). Maturation in hormone-substituted medium for 28 to 30 hours is recommended under the condition that immature oocytes were cultured from juvenile antral follicles (2--5 mm in diameter).</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"81-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of butorphanol on luteinizing hormone and follicle stimulating hormone levels in ovariectomized rats--comparative study with morphine sulphate. 丁托啡诺对去卵巢大鼠促黄体生成素和促卵泡激素水平的影响——与硫酸吗啡的比较研究。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
M Fayez, H H Ahmed, F el Nabarawy, I M Shokery, A M el-Bauomy
{"title":"Effects of butorphanol on luteinizing hormone and follicle stimulating hormone levels in ovariectomized rats--comparative study with morphine sulphate.","authors":"M Fayez,&nbsp;H H Ahmed,&nbsp;F el Nabarawy,&nbsp;I M Shokery,&nbsp;A M el-Bauomy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies were conducted into the effects on pituitary gonadotrophic hormones in ovariectomized rats of butorphanol, a synthetic morphine derivative which was claimed to be a potent analgesic with few side-effects, in comparison to effects of the naturally occurring alkaloid morphine. For this purpose, 3 groups of ovariectomized rats were used. Rats of the 1st group were injected butorphanol at 2 dose levels (1 or 2 mg/kg body weight [b.w.]. Those of the 2nd group were injected morphine sulphate (10 or 20 mg/kg b.w.). The 3rd group was injected saline and served as control. Blood samples were collected by orbital sinus punctures, just before treatment and 1 hour post injection. Luteinizing hormone (LH) and follicle-stimulation hormone levels were determined in the sera of rats by radio-immuno-assay. The results revealed that morphine, at the 2 dose levels used, produced more than 90% decrease in serum LH concentration, whereas butorphanol produced more than 70% decrease in serum LH levels. Both morphine and butorphanol, at the 2 doses used, produced more than 76% decrease in serum follicle stimulating hormone concentration. It is concluded that butorphanol, the morphinic derivative, has a depressive effect on the synthesis and/or release of gonadotrophic hormones. This inhibitory effect, however, was nearly as potent as that produced by morphine sulphate.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"101-3"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12953175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathomorphological changes in calf erythrocytes during phenylhydrazine-induced acute hemolytic anaemia. 苯肼引起的急性溶血性贫血期间小牛红细胞的病理形态学改变。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
M L Sharma, J L Soni, K Datta
{"title":"Pathomorphological changes in calf erythrocytes during phenylhydrazine-induced acute hemolytic anaemia.","authors":"M L Sharma,&nbsp;J L Soni,&nbsp;K Datta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In acute hemolytic anaemia of calves induced with phenylhydrazine-hydrochloride, accentuated degenerative changes in circulating erythrocytes occurred on the 5th d post-injection. Marked anisocytosis and poikilocytosis were observed. Crenated cells, acanthocytes (star-shaped cells), dacrocytes (tear drop cells), bite cells, and schistocytes predominated. Heinz bodies were the cytoplasmic hallmark of hemolytic anaemia. The regenerative nature of anaemia was evidenced by early appearance of reticulocytes in peripheral blood.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"45-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Estrus and ovulation synchronization in Merino meat sheep. 1. Effect of Gonavet "Berlin Chemie" after estrus synchronization with prostaglandin F2 alpha in Merino meat sheep]. 美利奴肉羊的发情和排卵同步。1. “柏林化学”对美利奴肉羊发情同步后前列腺素F2 α的影响[j]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
R Wolf, W Rommel, A Richter, H Bier, P Tschauschev
{"title":"[Estrus and ovulation synchronization in Merino meat sheep. 1. Effect of Gonavet \"Berlin Chemie\" after estrus synchronization with prostaglandin F2 alpha in Merino meat sheep].","authors":"R Wolf,&nbsp;W Rommel,&nbsp;A Richter,&nbsp;H Bier,&nbsp;P Tschauschev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oestrus synchronisation by means of PGF2 alpha analogues was followed by injection of Gonavet \"Berlin-Chemie\" which triggered off an LH peak, 2 to 3 hours from injection. Injection of Gonavet \"Berlin-Chemie\", 44 hours after PGF2 alpha application, caused synchronisation of all LH peaks. The interval between injection of Gonavet \"Berlin-Chemie\" and onset of ovulation amounted to 22 hours. The length of ovulation was not accurately determinable. Ovulation was successfully induced to all sheep by application of Gonavet \"Berlin-Chemie\", 44 or 48 hours after PGF2 alpha injection. Ovulation rates were 1.75 or 1.54. Luteolytic action on sheep of Cloprostenol \"Jenapharm\", a PGF2 alpha analogue, proved to be just as good as that of Oestrophan (SPOFA).</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The influence of follicular factors on the in vitro maturation of bovine oocytes]. [卵泡因子对牛卵母细胞体外成熟的影响]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
M Böttcher, G Koppitz, K Gernhard, W Lange
{"title":"[The influence of follicular factors on the in vitro maturation of bovine oocytes].","authors":"M Böttcher,&nbsp;G Koppitz,&nbsp;K Gernhard,&nbsp;W Lange","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immature oocytes from antral follicles of cattle were tested for the effect of follicular factors on maturation. In vitro maturation was accomplished by use of follicular fluid from small (2--5 mm) and large (above 15 mm) follicles and by addition to the medium of a granulose factor (GF) which had been isolated from the surface of granulosa cells. The parent material, with 84% (72/86) of oocytes at the germinal vesicle stage (GV-S) at the beginning of culturing, could be rated immature. 46% of all oocytes (41/89) had reached telophase I or metaphase II (full maturation) after 24 hours of maturation in hormone-free control medium (TCM 199 + 10% of foetal calf serum). 36% of oocytes (53/84), on the other hand, stayed between GV breakdown (GVBD) and anaphase I (incipient maturation). Full maturation was reached by as little as 14%. GF and follicular fluid from small antral follicles were found to inhibit GVBD in the oocytes. 59% (36/61) or 48% (61/127) of oocytes were blocked at GV stage. Positive determination of maturation inhibiting action of the above follicular components may provide a chance for their target-oriented use in control of the maturation process. The pool of immature oocytes of the ovaries, under such circumstances, might be more systematically utilised for in vitro manipulations.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"87-92"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The occurrence and the importance of enterotoxins from strains of Escherichia coli from bovine mastitis]. [牛乳腺炎大肠杆菌菌株肠毒素的发生及其重要性]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
O Alkaff, H Liebermann, A Bergmann
{"title":"[The occurrence and the importance of enterotoxins from strains of Escherichia coli from bovine mastitis].","authors":"O Alkaff,&nbsp;H Liebermann,&nbsp;A Bergmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Baby mouse and Y1 cell culturing tests were conducted into 117 Escherichia (E.) coli strains from mastitis of cattle to clear up enterotoxin formation. The rate of the latter was found to be relatively low, with enterotoxins recorded from only 7 strains (6%). No correlations were established between enterotoxin formation, on the one hand, and antibiotic resistance as well as biochemical activities of E. coli isolates, on the other. The question still remains open, if enterotoxin-forming strains derive their udder pathogenicity from endotoxins or with support by enterotoxins.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"149-54"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12952837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Orienting toxicologic studies in Wistar rats for the evaluation of knotgrass--(Polygonum convolvulus)--seed]. [在Wistar大鼠的毒理学研究中评价红叶草-(Polygonum convolvulus)-种子]
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
G Wilsdorf, R Heckel, J Juhrke
{"title":"[Orienting toxicologic studies in Wistar rats for the evaluation of knotgrass--(Polygonum convolvulus)--seed].","authors":"G Wilsdorf,&nbsp;R Heckel,&nbsp;J Juhrke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Black bindweed seed (Polygonum convolvulus) from the 1987 harvest was tested for possible toxicity in a 38-day range-finding test with Wistar rats which received feed rations on the basis of VDT-1 standard feedstuff and components of 5 or 20%. Histological liver findings as well as parameters recorded from laboratory diagnosis (plasma activities of ALAT, ASAT, and gamma GT as well as total protein and albumin levels in plasma) were indicative of incipient impairment of liver metabolism. More detailed and comprehensive chemico-analytical investigations are recommended, with due consideration of the unity between high-value primary components, on the one hand, and possibly toxic secondary constituent substances, on the other.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"123-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12952835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The acrosin system of bull, boar and ram sperm]. [公牛、公猪和公羊精子的顶体系统]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
H Nehring
{"title":"[The acrosin system of bull, boar and ram sperm].","authors":"H Nehring","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Described in this paper is a technique by which to separate the components of the sperma acrosin system. Included in the method are extraction of all components by means of acetic acid, separation of acrosin inhibitors on Sephadex G 100 as well as biochemical determination of proacrosin and acrosin. While species-related peculiarities were of minor importance, alterations were found to occur to the acrosin system in response to deep-freeze preservation of bull, boar, and ram sperma. Those alterations grew manifest primarily through decline in total acrosin activity and shifting of the proacrosin-acrosin ratio in the direction of proacrosin activation. Detachability of membrane-bound acrosin inhibitors was increased with significance, following in-vitro capacitation of bull sperma under heparin action.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Concentrations of prolactin, LH, estradiol-17 beta and progesterone in swine after biotechnical stimulation with PMSG, HCG and suidor]. [PMSG、HCG和suidor生物刺激后猪体内催乳素、LH、雌二醇-17 β和孕酮的浓度]。
Archiv fur experimentelle Veterinarmedizin Pub Date : 1991-01-01
E Kanitz, F Schneider, K P Brüssow, H E Schmidt, M Kitzig, G Blödow
{"title":"[Concentrations of prolactin, LH, estradiol-17 beta and progesterone in swine after biotechnical stimulation with PMSG, HCG and suidor].","authors":"E Kanitz,&nbsp;F Schneider,&nbsp;K P Brüssow,&nbsp;H E Schmidt,&nbsp;M Kitzig,&nbsp;G Blödow","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Concentrations of estradiol-17 beta, progesterone, luteinising hormone (LH), and prolactin were recorded from 9 gilts, following cycle blocking by means of Suisynchron(R)-Prämix and application of PMSG, HCG, and Suidor(R). A radio-immuno-assay which for its quality criteria enabled safe determination of the hormone in peripheral blood had been worked out specifically for prolactin appraisal. HCG application led to blockage of pre-ovulatory LH release in 7 of 9 animals. Possible causes are discussed. Prolactin concentrations during oestrus were differentiated and, for example, were characterised by strong oscillatory variations. With the experimental arrangement used, the boar pheromone Suidor(R) was not found to have any impact upon hormone profiles.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"45 1","pages":"55-66"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12951316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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