Applied Spectroscopy最新文献

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Multichannel Dilution Analysis Using a Single Peristaltic Pump Tube. 用单蠕动泵管进行多通道稀释分析。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-08-01 Epub Date: 2025-02-05 DOI: 10.1177/00037028251315753
Robbie M Huff, Willis B Jones, Bradley T Jones
{"title":"Multichannel Dilution Analysis Using a Single Peristaltic Pump Tube.","authors":"Robbie M Huff, Willis B Jones, Bradley T Jones","doi":"10.1177/00037028251315753","DOIUrl":"10.1177/00037028251315753","url":null,"abstract":"<p><p>Multi-channel dilution analysis (MCDA) is a novel, matrix-matched calibration method that automatically dilutes a standard solution using a tubing manifold to split a solution stream traveling between an autosampler and an analytical instrument. The manifold consists of separate tubing channels of various lengths, resulting in a stairstep of signal levels as different portions of the solution reach the instrument to be measured at different points in time. Multi-channel dilution analysis was initially developed on an inductively coupled plasma optical emission spectrometer (ICP-OES) equipped with a peristaltic pump with five channels, with three used to drive solution through individual tubes in the manifold and a fourth reserved for waste. The setup limited MCDA measurements from being realized on instruments that have fewer pump channels without employing an external pump. The use of a single peristaltic pump line to drive solution through the entire tubing manifold is proposed as a feasible way to overcome this hardware limitation and is applied to both ICP-OES and ICP mass spectrometry (ICP-MS). The method is validated through the analysis of certified reference materials, with recoveries for a suite of analytes ranging from 83% to 117%, with relative standard deviations on the order of 1%. The matrix matching capabilities of the method are demonstrated through the analysis of spiked matrices containing high concentrations of ethanol and calcium, and a comparison to traditional calibration techniques. In addition, the improvements in calibration statistics for ICP-MS measurements using MCDA are discussed.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"1256-1265"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding and Employing (Non-)Linearities in Attenuated Total Reflection Spectroscopy. 衰减全反射光谱学中(非线性)线性的理解和应用。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-08-01 Epub Date: 2025-02-13 DOI: 10.1177/00037028251317540
Thomas G Mayerhöfer, Jürgen Popp
{"title":"Understanding and Employing (Non-)Linearities in Attenuated Total Reflection Spectroscopy.","authors":"Thomas G Mayerhöfer, Jürgen Popp","doi":"10.1177/00037028251317540","DOIUrl":"10.1177/00037028251317540","url":null,"abstract":"<p><p>When Beer's law is interpreted through electromagnetic theory, it becomes clear that assuming a linear relationship between molar concentration and the imaginary part of the dielectric function is more accurate than using the absorption index function. A similar relationship holds true for attenuated total reflection (ATR) absorbance. When the negative logarithm of the reflectance is expanded into a series and truncated after the linear term, the approximation proves more accurate when based on the imaginary part of the dielectric function. Moreover, ATR correction schemes that utilize the low absorption approximation or the Bertie-Eysel formalism with this imaginary part, rather than the absorption index, tend to converge more quickly and provide more accurate results, particularly for stronger oscillators across an extended range of oscillator strength. Therefore, correction schemes for ATR spectra should prioritize the imaginary part of the dielectric function rather than the absorption index function when analyzing scalar and isotropic media.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"1290-1296"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-Precision and High-Resolution X-ray Fluorescence Analysis System for Chlorine Elements Based on Double-Curved Crystal Technology. 基于双曲线晶体技术的氯元素高精度高分辨率x射线荧光分析系统。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-08-01 Epub Date: 2025-03-26 DOI: 10.1177/00037028251324536
Chenyang Lu, Chong Huang, Xuefeng Wang, Qiang Li, Min Wu, Kun Wang, Zhaogui Liu
{"title":"High-Precision and High-Resolution X-ray Fluorescence Analysis System for Chlorine Elements Based on Double-Curved Crystal Technology.","authors":"Chenyang Lu, Chong Huang, Xuefeng Wang, Qiang Li, Min Wu, Kun Wang, Zhaogui Liu","doi":"10.1177/00037028251324536","DOIUrl":"10.1177/00037028251324536","url":null,"abstract":"<p><p>In X-ray fluorescence spectroscopy, specific elements, particularly neighboring elements, interfere with each other. A wavelength-dispersive optical system based on double-curved crystal (DCC) with multiple faces was designed. A Johann-type graphite curved crystal was used to focus the monochromatic excitation light, and a logarithmic spiral graphite curved crystal was used to collect the target fluorescence (Cl, 2.62  keV). The experimental results show that the wavelength dispersion device based on the DCC technology has a Cl detection limit of 0.18 part per million (ppm) when used for oil products. In the detection of a 1  ppm standard sample, the detection range of the device was 0.38  ppm, demonstrating good stability. Based on the fluorescence spectroscopy and detection results, the DCC technology could completely eliminate the influence of a high S concentration on Cl detection.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"1198-1205"},"PeriodicalIF":2.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hydrophobic Hydration and Light Transport in α-Synuclein Protein Solutions in the Near-Infrared. 近红外下α-突触核蛋白溶液的疏水水化和光传输。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-30 DOI: 10.1177/00037028251367004
Marco A Saraiva
{"title":"Hydrophobic Hydration and Light Transport in α-Synuclein Protein Solutions in the Near-Infrared.","authors":"Marco A Saraiva","doi":"10.1177/00037028251367004","DOIUrl":"10.1177/00037028251367004","url":null,"abstract":"<p><p>Currently, there is increasing interest in identifying the mechanistic characteristics of the α-synuclein amyloid protein aggregation during its early stages. The initiation of amyloid protein incubation was investigated by applying the concepts of hydrophobic hydration in the early-formed protein aggregates and the light transport in the protein samples by using near-infrared light. These are unexplored concepts in amyloid protein aggregation research. Early-formed protein aggregates develop solvent-exposed hydrophobic residue segments, and intramolecular and intermolecular interactions can be identified by hydrophobic hydration, while consecutive intramolecular interactions can cancel this effect. In the light transport within protein samples, at low protein concentrations, the early-formed protein aggregates achieve stability, whereas at higher concentrations, such as those found in neuronal synapses (∼50  µM), the early-formed aggregates continue to develop.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"37028251367004"},"PeriodicalIF":2.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Noncontact Fiber Optic Probe for Clinical Applications of Raman Spectroscopy. EXPRESS:用于拉曼光谱临床应用的非接触式光纤探针。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-30 DOI: 10.1177/00037028251367062
Sean Fitzgerald, Eric Marple, Jay Werkhaven, Anita Mahadevan-Jansen
{"title":"Noncontact Fiber Optic Probe for Clinical Applications of Raman Spectroscopy.","authors":"Sean Fitzgerald, Eric Marple, Jay Werkhaven, Anita Mahadevan-Jansen","doi":"10.1177/00037028251367062","DOIUrl":"10.1177/00037028251367062","url":null,"abstract":"<p><p>Clinical applications of Raman spectroscopy (RS) typically rely on fiber optic probes that directly interface with the tissue site. These devices are designed with small diameters, enabling them to navigate narrow body cavities and seamlessly integrate into routine medical instruments. However, the performance of conventional RS fiber probes suffers during noncontact operation due to decreased collection efficiency and a larger laser spot size that restricts spatial precision. To address these limitations, a novel RS probe design is presented here that can efficiently collect both fingerprint (FP) and high-wavenumber (HW) regions of the Raman spectrum at an offset from the target tissue using a miniature lens at the probe tip. The development process began with stochastic light propagation simulations that served as a foundation for the device's expected performance improvements compared to a standard RS probe design, which were then experimentally verified. Lenses were fabricated from various materials, including fused silica, quartz, sapphire, and calcium fluoride, to assess the impact of aberrant lens emissions on the analysis of tissue Raman features within the FP and HW spectral regions. Signal quality metrics are reported from in vivo tissue using each type of lens, demonstrating that crystalline lenses best preserve the weak Raman signal generated by tissues during dual-region RS analysis. Still, the ideal lens type will ultimately depend on material characteristics and which spectral region is required for tissue interrogation. This device demonstrated a 90% increase in signal intensity and a four-fold improvement in spatial selectivity compared to a conventional RS probe during noncontact operation. Finally, one embodiment of the noncontact probe is described to showcase a clinically compatible prototype, which incorporates a widefield camera module for positioning guidance during in vivo use.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"37028251367062"},"PeriodicalIF":2.2,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of β-Hydroxybutyric Acid Based on a Fluorescent Probe of Lanthanide Complexes. EXPRESS:基于镧系配合物荧光探针检测β-羟基丁酸。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-25 DOI: 10.1177/00037028251365665
XingYi Guo, Chun Zhu, Guoqing Chen, Lei Li, Chaoqun Ma, Taiqun Yang, Anqi Hu, WenHui Yang, YaLi Qian
{"title":"Detection of β-Hydroxybutyric Acid Based on a Fluorescent Probe of Lanthanide Complexes.","authors":"XingYi Guo, Chun Zhu, Guoqing Chen, Lei Li, Chaoqun Ma, Taiqun Yang, Anqi Hu, WenHui Yang, YaLi Qian","doi":"10.1177/00037028251365665","DOIUrl":"10.1177/00037028251365665","url":null,"abstract":"<p><p>In this paper, a lanthanide complex-based fluorescent sensor Tb(4-MBA) was developed for the selective recognition of diabetic ketoacidosis (DKA) and the diabetes biomarker β-hydroxybutyric (β-Hb). β-Hb significantly enhanced the fluorescence emission of the Tb(4-MBA) complex at 539  nm. Based on the analysis of the surface electrostatic potential distribution and time-resolved spectra, we speculate that in the reaction system of β-Hb with Tb(4-MBA), β-Hb and Tb(4-MBA) may form a complex through hydrogen bonding interactions, which brings β-Hb closer to Tb<sup>3+</sup> and thus reduces the non-radiative energy loss of the solvent molecules to Tb<sup>3+</sup> and significantly enhances the Tb(4-MBA) fluorescence intensity. The linear range of Tb(4-MBA) for β-Hb was 2-55 μM, and the limit of detection (LOD) was 50.6 nM. This sensor has high sensitivity and selectivity and shows great potential in the field of screening and diagnosis of diabetes mellitus and DKA.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"37028251365665"},"PeriodicalIF":2.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144706103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization and Identification of Diverse Fruit Rot Fungal Species Using Microscopic and Spectroscopic Approaches. 用显微和光谱方法鉴定不同果腐真菌种类。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-02 DOI: 10.1177/00037028251350655
Faizaa A Saif, Salama A Yaseen, Shrikant B Mane, Prabhakar B Undre, Nashi K Alqahtani
{"title":"Characterization and Identification of Diverse Fruit Rot Fungal Species Using Microscopic and Spectroscopic Approaches.","authors":"Faizaa A Saif, Salama A Yaseen, Shrikant B Mane, Prabhakar B Undre, Nashi K Alqahtani","doi":"10.1177/00037028251350655","DOIUrl":"https://doi.org/10.1177/00037028251350655","url":null,"abstract":"<p><p>Given fungi's critical role in public health and their impact during pandemics such as COVID-19, precise identification and classification are essential. Additionally, fungi hold significant value in medical and economic applications. For this work, fungi were isolated from various fruit. The fungi were initially identified based on their morphological characteristics using microscopic techniques. To achieve a comprehensive characterization, the eight fungal species were analyzed using rapid and cost-effective spectroscopic techniques, including attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR), Raman spectroscopy (RS), and ultraviolet-visible spectroscopy (UV-Vis). Fungal samples were used in the powder form, generating distinct spectral fingerprints in the biochemical region specific to components such as proteins, lipids, polysaccharides, carbohydrates, and nucleic acids. Results demonstrated the efficacy of these spectroscopic approaches for rapid and accurate identification, enabling discrimination between fungal species and reliable classification at the genus level. The results showed the species were identified as <i>Aspergillus parasiticus, Phytophthora spp., Chaetomium globosum, Penicillium digitatum, Penicillium sp., Penicillium italicum, Rhizoctonia solani</i>, and <i>Myrothecium roridum</i>. This highlights the potential of these techniques as efficient tools for fungi identification.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"37028251350655"},"PeriodicalIF":2.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Attenuated Total and Internal Reflection Correction Based on Fresnel's Equations: Beyond the Low Absorption Assumption. 基于菲涅耳方程的衰减全反射和内反射校正:超越低吸收假设。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-02 DOI: 10.1177/00037028251352565
Thomas G Mayerhöfer, Jürgen Popp
{"title":"Attenuated Total and Internal Reflection Correction Based on Fresnel's Equations: Beyond the Low Absorption Assumption.","authors":"Thomas G Mayerhöfer, Jürgen Popp","doi":"10.1177/00037028251352565","DOIUrl":"https://doi.org/10.1177/00037028251352565","url":null,"abstract":"<p><p>Some attenuated total reflection (ATR) correction formalisms share the drawback that they can only be applied to materials with relatively low oscillator strength, as they rely on one or another form of the low absorption assumption. In this contribution, we present an iterative formalism that does not suffer from this limitation and can be applied not only to attenuated total reflection spectra but also to spectra acquired under internal reflection conditions at subcritical incidence angles. Its accuracy is primarily limited near the critical angle and Brewster's angle. The formalism is based on the perpendicular component of the wavevector and Fresnel's equations, and it is fully compatible with wave optics. Its application is straightforward, and a corresponding program is available via the Supplemental Material.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"37028251352565"},"PeriodicalIF":2.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using Polarization to Increase Contrast of Water OH Raman Scattering Relative to Fluorescence of Dissolved Organic Matter. 利用极化提高水OH拉曼散射相对于溶解有机物荧光的对比度。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-01 Epub Date: 2024-12-16 DOI: 10.1177/00037028241303944
Callum E Twining, Paige K Williams, Whitney E Schuler, Caitlyn M English, Tammi L Richardson, Michael L Myrick
{"title":"Using Polarization to Increase Contrast of Water OH Raman Scattering Relative to Fluorescence of Dissolved Organic Matter.","authors":"Callum E Twining, Paige K Williams, Whitney E Schuler, Caitlyn M English, Tammi L Richardson, Michael L Myrick","doi":"10.1177/00037028241303944","DOIUrl":"10.1177/00037028241303944","url":null,"abstract":"<p><p>Environmental fluorescence measurements sometimes use water Raman scattering as an internal standard to compensate for path length, lensing effects, and turbidity. Fluorescent dissolved organic matter (FDOM) in water may interfere strongly with the measurement of this reference. However, fluorescence in fluid solution is largely unpolarized, while the OH stretching Raman band of water is always strongly polarized. Using an environmental sample from Lake Wateree in South Carolina, USA, we demonstrate that judicious use of this polarization allows for a significant level of improvement in the contrast or visibility of the water Raman band relative to FDOM.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"1164-1167"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Focusing Effects on Laser-Induced Plasma Parameters: Applications to a Graphite Target Under Martian Atmospheric Conditions. 激光诱导等离子体参数的聚焦效应:在火星大气条件下石墨靶的应用。
IF 2.2 3区 化学
Applied Spectroscopy Pub Date : 2025-07-01 Epub Date: 2025-01-02 DOI: 10.1177/00037028241307675
Kouider Benbaier, Ahmed Abdelmalek, Zeyneb Bedrane, Noureddine Melikechi
{"title":"Focusing Effects on Laser-Induced Plasma Parameters: Applications to a Graphite Target Under Martian Atmospheric Conditions.","authors":"Kouider Benbaier, Ahmed Abdelmalek, Zeyneb Bedrane, Noureddine Melikechi","doi":"10.1177/00037028241307675","DOIUrl":"10.1177/00037028241307675","url":null,"abstract":"<p><p>Under various atmospheric conditions, laser-induced breakdown spectroscopy (LIBS) is a powerful technique for elemental analysis, including in Earth- and Mars-like environments. However, understanding the plasma behavior and its dependence on ambient pressure and laser parameters remains a challenge. In this study, a numerical model based on a three-temperature Eulerian radiation framework under non-local thermodynamic equilibrium conditions is employed to investigate the interaction of a nanosecond laser pulse with a graphite target under helium (He) and carbon dioxide (CO<sub>2</sub> atmospheres. The aim is to provide insights into the effects of focusing conditions and ambient pressure (3 to 9 mbar and 1000 mbar) on plasma parameters relevant to both Earth- and Mars-like settings. Our results show that increased ambient pressure significantly enhances electron and ion densities, while the focusing conditions influence the temperature and fluid velocity of plasma species, as well as the spatial distribution and intensity of the plasma, ultimately affecting its diagnostic potential. These findings are critical for optimizing LIBS applications in planetary exploration and contribute to improving quantitative analyses under varying atmospheric compositions.</p>","PeriodicalId":8253,"journal":{"name":"Applied Spectroscopy","volume":" ","pages":"1102-1119"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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